Prupe.5G237400_v2.0.a1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001443
Physical Location & Seq
Reverse (-)
17954003 .. 17955352
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G237400.1

Sequence Viewer

Length: 1350 bp
ATGGTTTTAGGCTGGACAAGAAGGAGAACAGGCCACGGCACCAAGAAAAAGCAGCCCCGGGACCTAGACATGGAAGCCCTCATCCCAAACCACTTCCTGTGCCCAATATCGCTGGACCTGATGAAGGATCCGGTTACATTGTCATCCGGAATAACCTACGATAGACAGAGTATTGACACGTGGCTCGAAGCCGGGAATTTCACTTGCCCGGTAACAAATCAGGTGCTGAGAAACTTTGATCAGATTCCCAACCATAGTCTCAGAAGAATGATTCAAGAGTGGTGCACTCAAAACAAGCACTACGGCATCGAACGTGTCCCAACACCCAGAATTCCTGTCTTGCCAATGGAGGTCTCTGAGATTCTTTTCAGCATCGGAGCCTCTGCTAGACGTTTGGATCAACATGGCTGCTTAGAATGTGTGCACAAAATCAAGAAGTGGGGAGCTGAGAGCGAACGCAACAAGCGGTGCATCTCGGAGAATGGGACGGCCTCTGTTCTAGCTGGTGCCTTTGATTCTTTTGCAAGTGATTGCATAAAGAGAAATGCCACCGTTTGTGAAGAGATATTGTCTGCGCTGAGTTGGATGCTTCCAGCTTTCGATGAGGAAGCCCACAAATACTTGGGGTCTCAGGCTTCGTTGCACGGCATGGTTTGGTTTCTCAAGATGAGTGATGATCTTTCAGTAAAGCAAAATGCTATTTTAGCATTGAAAGAGCTTCTTGCTTGCCATGACCGGAATAAACATGTTGATGCGTTGGCGGAGATTGGTGGAGTGAATGAGGTACTTTTCGACTTCATCAGAGAGAAAATTTCTCCGACGATTACCAAGGCTTCACTGATGGTGGTCTTCTACTTGGTTTCATCATCATCATCTTCTTCTTCTTCTAAATCTAGTGAGAAGATCAAATCAGCATTTCTGGAGATGGGTTTAGTTTCCATATTGTTGGAAATTCTTGTCGACTCTGAAAGAGGCATATGTGAGAGGGCTTTGGGTGTTTTGGATAGTCTTTGCGATTTCCAAGATGGGAGGGAAAAGGCCTATGCTAATGCTCTCACTATACCTGTCTTGGTCAAGAAGATTTTGAGGGTATCAGAAATGGCGACTGAGTATTCAATTTCTGCTATTTGGAAGCTGTGCAAGTGTGCAAGCAGTCAAGAAGAAAGAGTTCTTGTTGAGGCCCTTCAAGTTGGTACATTTCAGAAGCTCCTGTTAGTTTTACAGGTTCGTTGCGGTGATGACAACACAAAGGAGAAGACAACTGAGCTTTTGAAACTCCTGAATCCTTACAGAGCTGGGTTGGAATGCATTGAGTCTGTAGATTTCAAGAACATTAGAAGGTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

450

Amino Acids

50.38

Weight (kDa)

7.1

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 38, 506
AccI GTMKAC 1 cut(s) 960
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 3 cut(s) 466, 761, 1233
AclWI GGATC 3 cut(s) 122, 135, 405
AcsI RAATTY 4 cut(s) 196, 330, 810, 951
AcvI CACGTG 1 cut(s) 180
AfaI GTAC 2 cut(s) 786, 1195
AfiI CCNNNNNNNGG 2 cut(s) 70, 124
AflIII ACRYGT 3 cut(s) 177, 313, 745
AgsI TTSAA 6 cut(s) 275, 712, 1116, 1187, 1273, 1327
AluBI AGCT 8 cut(s) 446, 503, 596, 718, 1135, 1207, 1267, 1295
AluI AGCT 8 cut(s) 446, 503, 596, 718, 1135, 1207, 1267, 1295
Alw21I GWGCWC 2 cut(s) 287, 426
Alw26I GTCTC 3 cut(s) 263, 358, 633
Alw44I GTGCAC 2 cut(s) 283, 422
AlwI GGATC 3 cut(s) 122, 135, 405
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 4 cut(s) 31, 489, 1038, 1179
ApaLI GTGCAC 2 cut(s) 283, 422
ApeKI GCWGC 2 cut(s) 52, 408
ApoI RAATTY 4 cut(s) 196, 330, 810, 951
Asp700I GAANNNNTTC 1 cut(s) 1167
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 61, 115, 1180
AsuC2I CCSGG 4 cut(s) 58, 59, 193, 209
AsuHPI GGTGA 1 cut(s) 1247
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 2 cut(s) 61, 115
BaeGI GKGCMC 3 cut(s) 104, 287, 426
BamHI GGATCC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 38, 506
BbrPI CACGTG 1 cut(s) 180
BbsI GAAGAC 2 cut(s) 841, 1262
Bbv12I GWGCWC 2 cut(s) 287, 426
BbvI GCAGC 2 cut(s) 64, 395
BccI CCATC 3 cut(s) 835, 919, 1019
BceAI ACGGC 4 cut(s) 52, 319, 504, 661
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 4 cut(s) 58, 59, 193, 209
BcoDI GTCTC 3 cut(s) 263, 358, 633
BfaI CTAG 4 cut(s) 65, 387, 500, 894
BfmI CTRYAG 1 cut(s) 1317
BisI GCNGC 2 cut(s) 53, 409
BlsI GCNGC 2 cut(s) 54, 410
Bme1390I CCNGG 4 cut(s) 58, 59, 193, 209
Bme18I GGWCC 2 cut(s) 61, 115
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 3 cut(s) 61, 115, 1180
BmiI GGNNCC 5 cut(s) 40, 62, 129, 379, 508
BmrFI CCNGG 4 cut(s) 58, 59, 193, 209
BmsI GCATC 5 cut(s) 315, 381, 480, 576, 742
BpiI GAAGAC 2 cut(s) 841, 1262
BpmI CTGGAG 1 cut(s) 941
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 4 cut(s) 58, 59, 193, 209
BsaAI YACGTR 1 cut(s) 180
BsaI GGTCTC 2 cut(s) 358, 633
BsaJI CCNNGG 4 cut(s) 34, 56, 57, 828
BsaWI WCCGGW 3 cut(s) 130, 146, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 124
BseAI TCCGGA 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 34, 56, 57, 828
BseGI GGATG 3 cut(s) 81, 143, 591
BseLI CCNNNNNNNGG 2 cut(s) 70, 124
BseMII CTCAG 8 cut(s) 218, 274, 348, 438, 569, 644, 1098, 1254
BseSI GKGCMC 3 cut(s) 104, 287, 426
BseXI GCAGC 2 cut(s) 64, 395
BseYI CCCAGC 1 cut(s) 1295
BshFI GGCC 4 cut(s) 33, 491, 1040, 1181
BshNI GGYRCC 2 cut(s) 38, 506
BsiHKAI GWGCWC 2 cut(s) 287, 426
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 6 cut(s) 58, 131, 147, 192, 209, 736
BslFI GGGAC 3 cut(s) 74, 302, 499
BslI CCNNNNNNNGG 2 cut(s) 70, 124
BsmAI GTCTC 3 cut(s) 263, 358, 633
BsmFI GGGAC 3 cut(s) 74, 302, 499
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 4 cut(s) 33, 491, 1040, 1181
Bso31I GGTCTC 2 cut(s) 358, 633
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 3 cut(s) 104, 287, 426
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 5 cut(s) 127, 238, 397, 676, 903
BspACI CCGC 3 cut(s) 466, 761, 1233
BspANI GGCC 4 cut(s) 33, 491, 1040, 1181
BspCNI CTCAG 8 cut(s) 219, 273, 349, 439, 570, 643, 1099, 1255
BspEI TCCGGA 1 cut(s) 146
BspLI GGNNCC 5 cut(s) 40, 62, 129, 379, 508
BspPI GGATC 3 cut(s) 122, 135, 405
BspT107I GGYRCC 2 cut(s) 38, 506
BspTNI GGTCTC 2 cut(s) 358, 633
BssECI CCNNGG 4 cut(s) 34, 56, 57, 828
BssMI GATC 5 cut(s) 127, 238, 397, 676, 903
BssT1I CCWWGG 1 cut(s) 828
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 727, 1150
BstDEI CTNAG 9 cut(s) 227, 260, 357, 412, 447, 578, 630, 1107, 1263
BstDSI CCRYGG 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 81, 143, 591
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 5 cut(s) 130, 241, 400, 679, 906
BstMAI GTCTC 3 cut(s) 263, 358, 633
BstMBI GATC 5 cut(s) 127, 238, 397, 676, 903
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 4 cut(s) 56, 57, 191, 207
BstSFI CTRYAG 1 cut(s) 1317
BstSLI GKGCMC 3 cut(s) 104, 287, 426
BstV1I GCAGC 2 cut(s) 64, 395
BstV2I GAAGAC 2 cut(s) 841, 1262
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 946
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 4 cut(s) 33, 491, 1040, 1181
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 3 cut(s) 81, 143, 591
BtsIMutI CAGTG 1 cut(s) 836
Cac8I GCNNGC 2 cut(s) 727, 1150
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 3 cut(s) 61, 115, 1180
Cfr9I CCCGGG 1 cut(s) 57
Csp6I GTAC 2 cut(s) 785, 1194
CviAII CATG 5 cut(s) 70, 404, 649, 731, 746
CviQI GTAC 2 cut(s) 785, 1194
DdeI CTNAG 9 cut(s) 227, 260, 357, 412, 447, 578, 630, 1107, 1263
DpnI GATC 5 cut(s) 129, 240, 399, 678, 905
DpnII GATC 5 cut(s) 127, 238, 397, 676, 903
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 776
Eco130I CCWWGG 1 cut(s) 828
Eco147I AGGCCT 1 cut(s) 1040
Eco31I GGTCTC 2 cut(s) 358, 633
Eco47I GGWCC 2 cut(s) 61, 115
Eco72I CACGTG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 57
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 2 cut(s) 61, 1180
EcoRI GAATTC 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 828
EcoT22I ATGCAT 1 cut(s) 1310
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 5 cut(s) 73, 407, 652, 734, 749
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 3 cut(s) 74, 302, 499
FatI CATG 5 cut(s) 69, 403, 648, 730, 745
FauNDI CATATG 1 cut(s) 977
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 960
Fnu4HI GCNGC 2 cut(s) 53, 409
FokI GGATG 3 cut(s) 68, 130, 598
Fsp4HI GCNGC 2 cut(s) 53, 409
FspBI CTAG 4 cut(s) 65, 387, 500, 894
GlaI GCGC 1 cut(s) 576
GluI GCNGC 2 cut(s) 53, 409
GsaI CCCAGC 1 cut(s) 1299
HaeIII GGCC 4 cut(s) 33, 491, 1040, 1181
HapII CCGG 6 cut(s) 58, 131, 147, 192, 209, 736
HhaI GCGC 1 cut(s) 577
Hin1II CATG 5 cut(s) 73, 407, 652, 734, 749
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 7 cut(s) 244, 271, 361, 515, 962, 1282, 1313
HpaII CCGG 6 cut(s) 58, 131, 147, 192, 209, 736
HphI GGTGA 1 cut(s) 1247
Hpy166II GTNNAC 3 cut(s) 285, 424, 961
Hpy188III TCNNGA 9 cut(s) 147, 275, 433, 664, 920, 1075, 1157, 1279, 1327
Hpy8I GTNNAC 3 cut(s) 285, 424, 961
Hpy99I CGWCG 1 cut(s) 823
HpyAV CCTTC 4 cut(s) 15, 118, 1193, 1332
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 3 cut(s) 179, 313, 391
HpyCH4V TGCA 9 cut(s) 285, 424, 471, 524, 534, 643, 1140, 1148, 1308
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 9 cut(s) 227, 260, 357, 412, 447, 578, 630, 1107, 1263
HpySE526I ACGT 3 cut(s) 179, 313, 391
Hsp92II CATG 5 cut(s) 73, 407, 652, 734, 749
HspAI GCGC 1 cut(s) 575
Kpn2I TCCGGA 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 127, 238, 397, 676, 903
LmnI GCTCC 3 cut(s) 377, 443, 1212
Lsp1109I GCAGC 2 cut(s) 64, 395
LweI GCATC 5 cut(s) 315, 381, 480, 576, 742
MaeI CTAG 4 cut(s) 65, 387, 500, 894
MaeII ACGT 3 cut(s) 179, 313, 391
MaeIII GTNAC 2 cut(s) 133, 211
MalI GATC 5 cut(s) 129, 240, 399, 678, 905
MboI GATC 5 cut(s) 127, 238, 397, 676, 903
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 3 cut(s) 104, 287, 426
MluCI AATT 5 cut(s) 196, 330, 810, 951, 1116
MlyI GAGTC 2 cut(s) 956, 1322
MmeI TCCRAC 4 cut(s) 563, 842, 927, 1281
Mph1103I ATGCAT 1 cut(s) 1310
MroI TCCGGA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 1167
MslI CAYNNNNRTG 1 cut(s) 750
MspI CCGG 6 cut(s) 58, 131, 147, 192, 209, 736
MspR9I CCNGG 4 cut(s) 58, 59, 193, 209
Mva1269I GAATGC 1 cut(s) 1310
MwoI GCNNNNNNNGC 1 cut(s) 704
NciI CCSGG 4 cut(s) 58, 59, 193, 209
NdeI CATATG 1 cut(s) 977
NdeII GATC 5 cut(s) 127, 238, 397, 676, 903
NlaIII CATG 5 cut(s) 73, 407, 652, 734, 749
NlaIV GGNNCC 5 cut(s) 40, 62, 129, 379, 508
NsiI ATGCAT 1 cut(s) 1310
NspI RCATGY 1 cut(s) 749
PceI AGGCCT 1 cut(s) 1040
PciI ACATGT 1 cut(s) 745
PctI GAATGC 1 cut(s) 1310
PdmI GAANNNNTTC 1 cut(s) 1167
PfeI GAWTC 5 cut(s) 244, 271, 361, 515, 1282
PkrI GCNGC 2 cut(s) 54, 410
PleI GAGTC 2 cut(s) 956, 1321
PmaCI CACGTG 1 cut(s) 180
PmlI CACGTG 1 cut(s) 180
PpsI GAGTC 2 cut(s) 956, 1321
Ppu21I YACGTR 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 61
PscI ACATGT 1 cut(s) 745
Psp5II RGGWCCY 1 cut(s) 61
PspCI CACGTG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 1295
PspN4I GGNNCC 5 cut(s) 40, 62, 129, 379, 508
PspPI GGNCC 3 cut(s) 61, 115, 1180
PspPPI RGGWCCY 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 2 cut(s) 786, 1195
RsaNI GTAC 2 cut(s) 785, 1194
RseI CAYNNNNRTG 1 cut(s) 750
SalI GTCGAC 1 cut(s) 959
SatI GCNGC 2 cut(s) 53, 409
Sau3AI GATC 5 cut(s) 127, 238, 397, 676, 903
Sau96I GGNCC 3 cut(s) 61, 115, 1180
SchI GAGTC 2 cut(s) 956, 1322
ScrFI CCNGG 4 cut(s) 58, 59, 193, 209
SduI GDGCHC 3 cut(s) 104, 287, 426
SfaNI GCATC 5 cut(s) 315, 381, 480, 576, 742
SfcI CTRYAG 1 cut(s) 1317
SinI GGWCC 2 cut(s) 61, 115
SmaI CCCGGG 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 5 cut(s) 196, 330, 810, 951, 1116
SseBI AGGCCT 1 cut(s) 1040
SsiI CCGC 3 cut(s) 466, 761, 1233
SspMI CTAG 4 cut(s) 65, 387, 500, 894
StuI AGGCCT 1 cut(s) 1040
StyD4I CCNGG 4 cut(s) 56, 57, 191, 207
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 3 cut(s) 182, 316, 394
TaqI TCGA 5 cut(s) 186, 309, 600, 792, 960
TasI AATT 5 cut(s) 196, 330, 810, 951, 1116
TfiI GAWTC 5 cut(s) 244, 271, 361, 515, 1282
TscAI CASTG 1 cut(s) 843
TseI GCWGC 2 cut(s) 52, 408
TspDTI ATGAA 3 cut(s) 137, 787, 852
TspMI CCCGGG 1 cut(s) 57
TspRI CASTG 1 cut(s) 843
VneI GTGCAC 2 cut(s) 283, 422
VpaK11BI GGWCC 2 cut(s) 61, 115
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 4 cut(s) 196, 330, 810, 951
XceI RCATGY 1 cut(s) 749
XmaI CCCGGG 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 960
XmnI GAANNNNTTC 1 cut(s) 1167
XspI CTAG 4 cut(s) 65, 387, 500, 894
Zsp2I ATGCAT 1 cut(s) 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.