Rw7G000840

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001443
Physical Location & Seq
Forward (+)
666544 .. 667971
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000840.1

Sequence Viewer

Length: 1323 bp
ATGGTTTTGGGTTGGAGAAGAAGGAGCAGCACCGGCCACCGTGCCGCCGCTGCGAAAAAGAAGCCTTCACCTGGCAACCTCGACATGGATCAACTCGTGATCCCAAAGCATTTCCTCTGCCCAATATCGCTCGACTTGATGAAGGATCCGGTCACATTGTCCTCAGGGATAACTTATGATCGGCAGAGCATTGACACTTGGCTCGAAGCCGGCAACTTCAAGTGCCCGGTGACCAATCAGGTGCTGAGGAACTTTGATATGATTCCCAACCACACTCTCCGAACAATGATTCAAGATTGGAGCGCTCAAAACCAACGCTACGGCATTCAGCGTGTCCCAACTCCTAGAATTCCCATCATGGCAGTTGAGGTCTCTGAGATTCTGTTTAGCATCACAGCCTCAGCGAAACGCGTCGATCAAAATGGTTGCTTAGAGTGCGTGCAGATAATCAAGAAATGGGCGGCTGAGGGCGACCGAAACAAGCGGTGCATCGTGGAGAATGGAGCGGCCTCTGTTCTTGCTTCTGCTTTTGATGCTTTTGCAAGTGATGGAATAAAGAAAAATGCTGGAGTGTGTGAAGAAATTTTGTCAGCTCTGAGTTGGATGATGTTTCCATCATTAGATGAAGAAACCCAAAAGTACTTGGGATCTCATGCTTCTTTGGATTGCCTGGTTTGGTTCCTCAAGGTTGATGGAGATCTTTTGGTGAAGCAAAACTCAATTCTAGCATTGAAAGAGCTTCTTTCTAATAGCAATGATCAGAACAAACATGCGGTTGAAGCATTGGCAGAGATTGAAGGAGTCAATAAGATACTTTTCGGGTTCATCAAGGAGAAAAGTTCCATGACGATTACCAAGTCTTCTTTAACAAAGTTGAGATCAGCATTCTTGGAGATGGGTTTGGTTTCCCTATTGTTAGAGACCCTTCTCGACTCGGAAAAAGGCGTAATGGAGAGGGCTTTGTATGTTTTGGATTGTCTTTGTGATTGCAAAGAAGGGAGGGAACAGGCCTATGCTAATGCGCTGACTATACCAGTTTTGGTGAAGAAGATTTTCAGGGTGTCAGAAATGGCGACCGAGTATTCGATCTCTGCTGTTTGGAAGCTGTGTAAGAATGCAACTATGCAAGAAGAGAGAGTACTTGTTGAGGCTCTTCAAGTTGGTGCATTTCAGAAGCTCTTGCTGGTTTTACAGGTCCATTGCGCCGGTGAATATGACACTAAGGACAAACCAACTGAGCTTTTGAAGCTGTTGAATCCTTACAGAGCTGGATTGGAATGCATCGAGTCTGTAGATTTCAAGAGTATTAAAAGGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

440

Amino Acids

49.15

Weight (kDa)

7.46

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 34 - 105 1.4e-21 U-box domain
ARM_PUB PF25598 124 - 435 3.8e-58 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 506
AccII CGCG 1 cut(s) 411
AciI CCGC 6 cut(s) 45, 48, 461, 484, 506, 773
AclWI GGATC 5 cut(s) 94, 96, 140, 153, 655
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 348, 582
AfaI GTAC 2 cut(s) 641, 1140
AfeI AGCGCT 1 cut(s) 304
AfiI CCNNNNNNNGG 2 cut(s) 71, 85
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 9 cut(s) 220, 293, 733, 779, 797, 1157, 1246, 1255, 1300
AjnI CCWGG 2 cut(s) 70, 669
AluBI AGCT 7 cut(s) 593, 739, 1105, 1177, 1240, 1249, 1268
AluI AGCT 7 cut(s) 593, 739, 1105, 1177, 1240, 1249, 1268
Alw26I GTCTC 2 cut(s) 376, 914
AlwI GGATC 5 cut(s) 94, 96, 140, 153, 655
AlwNI CAGNNNCTG 1 cut(s) 244
Aor51HI AGCGCT 1 cut(s) 304
AoxI GGCC 3 cut(s) 34, 507, 1008
ApeKI GCWGC 2 cut(s) 27, 50
ApoI RAATTY 2 cut(s) 348, 582
ArsI GACNNNNNNTTYG 2 cut(s) 1066, 1098
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 3 cut(s) 305, 1024, 1205
AspS9I GGNCC 1 cut(s) 1195
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 5 cut(s) 60, 241, 718, 1054, 1220
AvaII GGWCC 1 cut(s) 1195
AxyI CCTNAGG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 227
BamHI GGATCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 1122, 1154
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 852
BbvCI CCTCAGC 3 cut(s) 245, 400, 465
BbvI GCAGC 2 cut(s) 37, 39
BccI CCATC 5 cut(s) 362, 542, 622, 686, 889
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 2 cut(s) 72, 671
BclI TGATCA 1 cut(s) 757
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 376, 914
BfaI CTAG 2 cut(s) 345, 725
BfmI CTRYAG 1 cut(s) 1290
BfoI RGCGCY 1 cut(s) 306
BglII AGATCT 1 cut(s) 697
BisI GCNGC 6 cut(s) 28, 45, 48, 51, 462, 507
BlsI GCNGC 6 cut(s) 29, 46, 49, 52, 463, 508
BmcAI AGTACT 2 cut(s) 641, 1140
Bme1390I CCNGG 3 cut(s) 72, 227, 671
Bme18I GGWCC 1 cut(s) 1195
BmgT120I GGNCC 1 cut(s) 1195
BmiI GGNNCC 2 cut(s) 147, 680
BmrFI CCNGG 3 cut(s) 72, 227, 671
BmsI GCATC 4 cut(s) 399, 498, 523, 1290
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 3 cut(s) 245, 400, 465
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 696
BsaI GGTCTC 2 cut(s) 376, 914
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 85
Bse118I RCCGGY 3 cut(s) 32, 209, 1205
Bse1I ACTGG 1 cut(s) 1034
Bse21I CCTNAGG 1 cut(s) 163
Bse3DI GCAATG 2 cut(s) 760, 1198
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 2 cut(s) 72, 671
BseGI GGATG 1 cut(s) 609
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 2 cut(s) 71, 85
BseMI GCAATG 2 cut(s) 760, 1198
BseMII CTCAG 7 cut(s) 177, 236, 366, 414, 456, 587, 1227
BseNI ACTGG 1 cut(s) 1034
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 2 cut(s) 37, 39
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 2 cut(s) 475, 1077
BshFI GGCC 3 cut(s) 36, 509, 1010
BsiEI CGRYCG 2 cut(s) 475, 1077
BsiSI CCGG 5 cut(s) 33, 149, 210, 227, 1206
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 71, 85
BsmAI GTCTC 2 cut(s) 376, 914
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 4 cut(s) 324, 884, 1120, 1283
BsnI GGCC 3 cut(s) 36, 509, 1010
Bso31I GGTCTC 2 cut(s) 376, 914
Bsp1286I GDGCHC 1 cut(s) 227
BspACI CCGC 6 cut(s) 45, 48, 461, 484, 506, 773
BspANI GGCC 3 cut(s) 36, 509, 1010
BspCNI CTCAG 7 cut(s) 176, 237, 367, 413, 457, 588, 1228
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 2 cut(s) 147, 680
BspPI GGATC 5 cut(s) 94, 96, 140, 153, 655
BspQI GCTCTTC 1 cut(s) 1158
BspTNI GGTCTC 2 cut(s) 376, 914
BsrBI CCGCTC 1 cut(s) 506
BsrDI GCAATG 2 cut(s) 760, 1198
BsrFI RCCGGY 3 cut(s) 32, 209, 1205
BsrI ACTGG 1 cut(s) 1034
BssAI RCCGGY 3 cut(s) 32, 209, 1205
BssSI CACGAG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 72, 671
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 2 cut(s) 1125, 1158
BstC8I GCNNGC 2 cut(s) 211, 440
BstDEI CTNAG 9 cut(s) 163, 245, 375, 400, 430, 465, 596, 1221, 1236
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 1 cut(s) 609
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 3 cut(s) 305, 1024, 1205
BstMAI GTCTC 2 cut(s) 376, 914
BstMCI CGRYCG 2 cut(s) 475, 1077
BstMWI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1246
BstNI CCWGG 2 cut(s) 72, 671
BstNSI RCATGY 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 3 cut(s) 70, 225, 669
BstSFI CTRYAG 1 cut(s) 1290
BstSLI GKGCMC 1 cut(s) 227
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 2 cut(s) 37, 39
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 3 cut(s) 145, 647, 697
BstYI RGATCY 3 cut(s) 145, 647, 697
Bsu36I CCTNAGG 1 cut(s) 163
BsuRI GGCC 3 cut(s) 36, 509, 1010
BtsCI GGATG 1 cut(s) 609
Cac8I GCNNGC 2 cut(s) 211, 440
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 3 cut(s) 305, 1024, 1205
Cfr10I RCCGGY 3 cut(s) 32, 209, 1205
Cfr13I GGNCC 1 cut(s) 1195
CseI GACGC 1 cut(s) 400
Csp6I GTAC 2 cut(s) 640, 1139
CviAII CATG 5 cut(s) 85, 358, 653, 770, 844
CviQI GTAC 2 cut(s) 640, 1139
DdeI CTNAG 9 cut(s) 163, 245, 375, 400, 430, 465, 596, 1221, 1236
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 1125, 1158
EarI CTCTTC 2 cut(s) 1125, 1158
Eco147I AGGCCT 1 cut(s) 1010
Eco31I GGTCTC 2 cut(s) 376, 914
Eco47I GGWCC 1 cut(s) 1195
Eco47III AGCGCT 1 cut(s) 304
Eco81I CCTNAGG 1 cut(s) 163
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 70, 669
EcoT22I ATGCAT 1 cut(s) 1283
FaeI CATG 5 cut(s) 88, 361, 656, 773, 847
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 320
FatI CATG 5 cut(s) 84, 357, 652, 769, 843
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 6 cut(s) 28, 45, 48, 51, 462, 507
FokI GGATG 1 cut(s) 616
Fsp4HI GCNGC 6 cut(s) 28, 45, 48, 51, 462, 507
FspBI CTAG 2 cut(s) 345, 725
GlaI GCGC 3 cut(s) 304, 1023, 1204
GluI GCNGC 6 cut(s) 28, 45, 48, 51, 462, 507
GsuI CTGGAG 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 3 cut(s) 36, 509, 1010
HapII CCGG 5 cut(s) 33, 149, 210, 227, 1206
HgaI GACGC 1 cut(s) 400
HhaI GCGC 3 cut(s) 305, 1024, 1205
Hin1II CATG 5 cut(s) 88, 361, 656, 773, 847
Hin6I GCGC 3 cut(s) 303, 1022, 1203
HinP1I GCGC 3 cut(s) 303, 1022, 1203
HinfI GANTC 7 cut(s) 262, 289, 379, 801, 932, 1255, 1286
HpaII CCGG 5 cut(s) 33, 149, 210, 227, 1206
HphI GGTGA 5 cut(s) 60, 241, 718, 1054, 1220
Hpy188I TCNGA 8 cut(s) 281, 376, 597, 762, 937, 1066, 1173, 1322
Hpy188III TCNNGA 5 cut(s) 97, 293, 451, 929, 1300
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 6 cut(s) 15, 75, 136, 791, 935, 989
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4V TGCA 8 cut(s) 442, 489, 542, 990, 1118, 1126, 1166, 1281
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1246
HpyF3I CTNAG 9 cut(s) 163, 245, 375, 400, 430, 465, 596, 1221, 1236
Hsp92II CATG 5 cut(s) 88, 361, 656, 773, 847
HspAI GCGC 3 cut(s) 303, 1022, 1203
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 757
LguI GCTCTTC 1 cut(s) 1158
LmnI GCTCC 3 cut(s) 24, 300, 503
Lsp1109I GCAGC 2 cut(s) 37, 39
LweI GCATC 4 cut(s) 399, 498, 523, 1290
MaeI CTAG 2 cut(s) 345, 725
MaeIII GTNAC 2 cut(s) 151, 229
MbiI CCGCTC 1 cut(s) 506
MboII GAAGA 8 cut(s) 30, 590, 638, 852, 1057, 1060, 1142, 1145
MflI RGATCY 3 cut(s) 145, 647, 697
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 3 cut(s) 348, 582, 720
MluI ACGCGT 1 cut(s) 409
MlyI GAGTC 3 cut(s) 810, 926, 1295
MmeI TCCRAC 1 cut(s) 581
Mph1103I ATGCAT 1 cut(s) 1283
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 1052
MseI TTAA 2 cut(s) 866, 1308
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 5 cut(s) 33, 149, 210, 227, 1206
MspR9I CCNGG 3 cut(s) 72, 227, 671
Mva1269I GAATGC 4 cut(s) 324, 884, 1120, 1283
MvaI CCWGG 2 cut(s) 72, 671
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1246
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 227
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 5 cut(s) 88, 361, 656, 773, 847
NlaIV GGNNCC 2 cut(s) 147, 680
NmuCI GTSAC 2 cut(s) 151, 229
NsiI ATGCAT 1 cut(s) 1283
NspI RCATGY 1 cut(s) 773
PceI AGGCCT 1 cut(s) 1010
PciSI GCTCTTC 1 cut(s) 1158
PctI GAATGC 4 cut(s) 324, 884, 1120, 1283
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 1052
PfeI GAWTC 4 cut(s) 262, 289, 379, 1255
PkrI GCNGC 6 cut(s) 29, 46, 49, 52, 463, 508
PleI GAGTC 3 cut(s) 809, 926, 1294
PpsI GAGTC 3 cut(s) 809, 926, 1294
Psp6I CCWGG 2 cut(s) 70, 669
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 2 cut(s) 70, 669
PspN4I GGNNCC 2 cut(s) 147, 680
PspPI GGNCC 1 cut(s) 1195
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 3 cut(s) 145, 647, 697
RsaI GTAC 2 cut(s) 641, 1140
RsaNI GTAC 2 cut(s) 640, 1139
SapI GCTCTTC 1 cut(s) 1158
SaqAI TTAA 2 cut(s) 866, 1308
SatI GCNGC 6 cut(s) 28, 45, 48, 51, 462, 507
Sau96I GGNCC 1 cut(s) 1195
ScaI AGTACT 2 cut(s) 641, 1140
SchI GAGTC 3 cut(s) 810, 926, 1295
ScrFI CCNGG 3 cut(s) 72, 227, 671
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 4 cut(s) 399, 498, 523, 1290
SfcI CTRYAG 1 cut(s) 1290
SgrAI CRCCGGYG 1 cut(s) 1205
SinI GGWCC 1 cut(s) 1195
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 3 cut(s) 348, 582, 720
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 6 cut(s) 45, 48, 461, 484, 506, 773
SspMI CTAG 2 cut(s) 345, 725
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 3 cut(s) 70, 225, 669
TaaI ACNGT 1 cut(s) 41
TaqI TCGA 7 cut(s) 81, 132, 204, 414, 930, 1085, 1284
TaqII GACCGA 2 cut(s) 489, 1091
TasI AATT 3 cut(s) 348, 582, 720
TatI WGTACW 2 cut(s) 639, 1138
TauI GCSGC 4 cut(s) 47, 50, 464, 509
TfiI GAWTC 4 cut(s) 262, 289, 379, 1255
Tru1I TTAA 2 cut(s) 866, 1308
Tru9I TTAA 2 cut(s) 866, 1308
TseFI GTSAC 2 cut(s) 151, 229
TseI GCWGC 2 cut(s) 27, 50
Tsp45I GTSAC 2 cut(s) 151, 229
TspDTI ATGAA 3 cut(s) 155, 639, 814
VpaK11BI GGWCC 1 cut(s) 1195
XapI RAATTY 2 cut(s) 348, 582
XceI RCATGY 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 2 cut(s) 345, 725
ZrmI AGTACT 2 cut(s) 641, 1140
Zsp2I ATGCAT 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.