RchiOBHm_Chr3g0492391

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001443
Physical Location & Seq
Reverse (-)
39142969 .. 39144552
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45528

Sequence Viewer

Length: 1350 bp
ATGATTTTGTCGTGGAGGAGAAGAAGGGCGAGCAAGTATGCCAACAGGAAGGAGTTTACCGGCGAGACTCTAGACATGGGGATGGAGGAGATCGTGATTCCATCCCACTTCCGGTGCCCTATTACTCTGGACCTCATGAAAGATCCGGTCACTTTGTCGACTGGGATGACCTACGACAGGTCCAGCATCGACGCCTGGATCGAGAAAGGGAACAAGACCTGCCCGGTAACCAATCAAGTTCTGGCAACGTTCGACCAAATCCCCAACCACGCGCTACGTAAGATGATCCAAGACTGGTGCGTCGAGAACCGCTCTCACGGCGTGGAACGCATCCCCACGCCTCGGATTCCGGTGTCTGCGTACGAGGTTACTAGGGTTTATCAGAGAATCATGGCCGCGACTCAACGCGGCGATTACAACAAGTGTTTAGAGTTGGTGGCCAAGGTCAGAGCTTGGGGAAAAGACAGCGAGCGTAACAAGAAGTGTATGATGGAGAACAGAATGGGCTGCTGCTTGTCGGCCACTTTCGAGTTCTTCGCCGGAAATGAGGAAACAACGTTGTTGGAGGAGATCTTGGCCGTGCTGCCATGGATGTTTCCCATTGGTGAAGAAGGCCAATCAAAGCTTGGATCTGCAAAATCGTTGAGATGTTTGGTGTGGTTTTTGGAGGGAAAAGATCTCTCTTCAAGGCAAAACGCAGTTTCGGTACTGAGAAAGTTGGTTTCTTTGGATCAAAAACATGTGGATGCGATTATGGAGATTGAAGGAGCCATTGAAGCATTGGTGAAGATAATTAGGGAGGGCCCTAGTGTCTGTCCTACAGCAACTAAAGCTTCTCTATCCTGTGTCTTCTACATGATTTCAGGAGCAAGTAATGAAATCAAATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGTTGCTAGAGATGCTTGTTGATGCAGAGAAGAGCCTATGTGAGAGGGCTTTGGGGGTTTTGGAAGGCCTTTGTGATTGCAAGCAAGGGAGAGAAAAGGCCTACGAGAATGCTCTAACCATGCCTCTTTTGGTTAAGAAGATGCTAAGGGTATCAGTCTTGGCGAATGAGTTTTCGGTTTCTATAATTTGGAAGCTCTGCAGGAACCAAAATGGTGATGGTGATGGTGATGACGAGGAAGAAATTAAGATTTTGATGGAGGCACTTCAAGTGGGCGCATTTCAGAAGTTCTTGGTTCTGTTGCAGGTGGGGTGTGGTGATGGAATCAAGGACAAGGTTACTGAGTTGTTGAAGTTGTTCAATCTTCACATCAGTAAAGTAGACTGTGTTGATTCATCCATGGATTTTAAGTATCTGAAGAAAGGCCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

449

Amino Acids

50.58

Weight (kDa)

6.94

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 33 - 104 4.7e-22 U-box domain
ARM_PUB PF25598 122 - 443 5.6e-73 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1210
AasI GACNNNNNNGTC 1 cut(s) 299
Acc36I ACCTGC 2 cut(s) 227, 1210
AccB1I GGYRCC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 158, 1296
AccII CGCG 3 cut(s) 272, 398, 408
AciI CCGC 3 cut(s) 310, 396, 408
AclI AACGTT 2 cut(s) 248, 557
AclWI GGATC 5 cut(s) 137, 206, 280, 637, 738
AcoI YGGCCR 4 cut(s) 393, 438, 519, 576
AcyI GRCGYC 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 2 cut(s) 362, 708
AfiI CCNNNNNNNGG 3 cut(s) 111, 177, 342
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 6 cut(s) 687, 764, 776, 1184, 1267, 1276
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 4 cut(s) 452, 625, 833, 1111
AluI AGCT 4 cut(s) 452, 625, 833, 1111
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 5 cut(s) 137, 206, 280, 637, 738
AoxI GGCC 9 cut(s) 393, 438, 519, 576, 613, 802, 982, 1014, 1339
ApaI GGGCCC 1 cut(s) 806
ApeKI GCWGC 3 cut(s) 507, 510, 583
Asp700I GAANNNNTTC 1 cut(s) 1271
AspLEI GCGC 2 cut(s) 274, 1193
AspS9I GGNCC 4 cut(s) 130, 180, 802, 803
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 6 cut(s) 617, 796, 1142, 1148, 1154, 1244
AvaII GGWCC 2 cut(s) 130, 180
BaeGI GKGCMC 2 cut(s) 119, 806
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 806
BbsI GAAGAC 1 cut(s) 841
BbvI GCAGC 3 cut(s) 494, 497, 570
BccI CCATC 7 cut(s) 76, 109, 484, 1127, 1133, 1165, 1229
BceAI ACGGC 2 cut(s) 334, 563
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 4 cut(s) 71, 372, 807, 923
BfmI CTRYAG 2 cut(s) 819, 1114
BfuAI ACCTGC 2 cut(s) 227, 1210
BglII AGATCT 2 cut(s) 570, 676
BisI GCNGC 5 cut(s) 396, 409, 508, 511, 584
BlsI GCNGC 5 cut(s) 397, 410, 509, 512, 585
Bme1390I CCNGG 2 cut(s) 196, 224
Bme18I GGWCC 2 cut(s) 130, 180
BmgT120I GGNCC 4 cut(s) 130, 180, 802, 803
BmiI GGNNCC 4 cut(s) 116, 769, 804, 1121
BmrFI CCNGG 2 cut(s) 196, 224
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 841
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu10I CCTNAGC 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 341, 441, 587, 1314
BsaWI WCCGGW 3 cut(s) 111, 145, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 177, 342
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 166, 299
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 4 cut(s) 341, 441, 587, 1314
BseGI GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BseLI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BseMII CTCAG 2 cut(s) 701, 1248
BseNI ACTGG 2 cut(s) 166, 299
BseRI GAGGAG 3 cut(s) 31, 101, 581
BseSI GKGCMC 2 cut(s) 119, 806
BseXI GCAGC 3 cut(s) 494, 497, 570
Bsh1236I CGCG 3 cut(s) 272, 398, 408
BshFI GGCC 9 cut(s) 395, 440, 521, 578, 615, 804, 984, 1016, 1341
BshNI GGYRCC 1 cut(s) 114
BsiSI CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
BsiWI CGTACG 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BsmAI GTCTC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 9 cut(s) 395, 440, 521, 578, 615, 804, 984, 1016, 1341
Bsp120I GGGCCC 1 cut(s) 802
Bsp1286I GDGCHC 2 cut(s) 119, 806
Bsp143I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
Bsp19I CCATGG 2 cut(s) 587, 1314
BspACI CCGC 3 cut(s) 310, 396, 408
BspANI GGCC 9 cut(s) 395, 440, 521, 578, 615, 804, 984, 1016, 1341
BspCNI CTCAG 2 cut(s) 702, 1249
BspFNI CGCG 3 cut(s) 272, 398, 408
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 116, 769, 804, 1121
BspMAI CTGCAG 1 cut(s) 1118
BspMI ACCTGC 2 cut(s) 227, 1210
BspPI GGATC 5 cut(s) 137, 206, 280, 637, 738
BspQI GCTCTTC 1 cut(s) 941
BspT107I GGYRCC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 166, 299
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 4 cut(s) 341, 441, 587, 1314
BssMI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 441, 587, 1314
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 1301
Bst6I CTCTTC 2 cut(s) 688, 941
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 31, 470, 998
BstDEI CTNAG 3 cut(s) 710, 1061, 1257
BstDSI CCRYGG 2 cut(s) 587, 1314
BstEII GGTNACC 1 cut(s) 226
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BstFNI CGCG 3 cut(s) 272, 398, 408
BstHHI GCGC 2 cut(s) 274, 1193
BstKTI GATC 8 cut(s) 93, 145, 201, 288, 573, 632, 679, 733
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
BstMWI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 743
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 194, 222
BstSFI CTRYAG 2 cut(s) 819, 1114
BstSLI GKGCMC 2 cut(s) 119, 806
BstSNI TACGTA 1 cut(s) 278
BstUI CGCG 3 cut(s) 272, 398, 408
BstV1I GCAGC 3 cut(s) 494, 497, 570
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 4 cut(s) 142, 570, 629, 676
BstYI RGATCY 4 cut(s) 142, 570, 629, 676
BsuRI GGCC 9 cut(s) 395, 440, 521, 578, 615, 804, 984, 1016, 1341
BtgI CCRYGG 2 cut(s) 587, 1314
BtsCI GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BveI ACCTGC 2 cut(s) 227, 1210
Cac8I GCNNGC 3 cut(s) 31, 470, 998
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 274, 1193
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 4 cut(s) 130, 180, 802, 803
CseI GACGC 2 cut(s) 200, 289
Csp6I GTAC 2 cut(s) 361, 707
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 8 cut(s) 76, 136, 391, 588, 740, 856, 1036, 1315
CviQI GTAC 2 cut(s) 361, 707
DdeI CTNAG 3 cut(s) 710, 1061, 1257
DpnI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 678, 732
DpnII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
DraIII CACNNNGTG 1 cut(s) 322
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 4 cut(s) 393, 438, 519, 576
Eam1104I CTCTTC 2 cut(s) 688, 941
EarI CTCTTC 2 cut(s) 688, 941
Eco105I TACGTA 1 cut(s) 278
Eco130I CCWWGG 3 cut(s) 441, 587, 1314
Eco147I AGGCCT 2 cut(s) 984, 1016
Eco24I GRGCYC 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 130, 180
Eco91I GGTNACC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 802, 803
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 3 cut(s) 441, 587, 1314
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 3 cut(s) 441, 587, 1314
FaeI CATG 8 cut(s) 79, 139, 394, 591, 743, 859, 1039, 1318
FatI CATG 8 cut(s) 75, 135, 390, 587, 739, 855, 1035, 1314
FblI GTMKAC 2 cut(s) 158, 1296
Fnu4HI GCNGC 5 cut(s) 396, 409, 508, 511, 584
FokI GGATG 7 cut(s) 88, 94, 178, 317, 604, 758, 1297
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 5 cut(s) 396, 409, 508, 511, 584
FspBI CTAG 4 cut(s) 71, 372, 807, 923
GlaI GCGC 2 cut(s) 273, 1192
GluI GCNGC 5 cut(s) 396, 409, 508, 511, 584
HaeIII GGCC 9 cut(s) 395, 440, 521, 578, 615, 804, 984, 1016, 1341
HapII CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
HgaI GACGC 2 cut(s) 200, 289
HhaI GCGC 2 cut(s) 274, 1193
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 8 cut(s) 79, 139, 394, 591, 743, 859, 1039, 1318
Hin6I GCGC 2 cut(s) 272, 1191
HinP1I GCGC 2 cut(s) 272, 1191
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 2 cut(s) 623, 831
HinfI GANTC 7 cut(s) 67, 97, 346, 387, 400, 1239, 1307
HpaII CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
HphI GGTGA 6 cut(s) 617, 796, 1142, 1148, 1154, 1244
Hpy166II GTNNAC 3 cut(s) 57, 159, 1297
Hpy188I TCNGA 5 cut(s) 345, 384, 449, 1200, 1332
Hpy188III TCNNGA 8 cut(s) 71, 94, 128, 136, 202, 304, 864, 888
Hpy8I GTNNAC 3 cut(s) 57, 159, 1297
Hpy99I CGWCG 2 cut(s) 194, 305
HpyAV CCTTC 5 cut(s) 18, 43, 605, 758, 974
HpyCH4III ACNGT 1 cut(s) 1301
HpyCH4IV ACGT 3 cut(s) 248, 277, 557
HpyCH4V TGCA 5 cut(s) 635, 941, 996, 1116, 1219
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
HpyF3I CTNAG 3 cut(s) 710, 1061, 1257
HpySE526I ACGT 3 cut(s) 248, 277, 557
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 8 cut(s) 79, 139, 394, 591, 743, 859, 1039, 1318
HspAI GCGC 2 cut(s) 272, 1191
Kzo9I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
LguI GCTCTTC 1 cut(s) 941
LmnI GCTCC 2 cut(s) 767, 866
Lsp1109I GCAGC 3 cut(s) 494, 497, 570
LweI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
MaeI CTAG 4 cut(s) 71, 372, 807, 923
MaeII ACGT 3 cut(s) 248, 277, 557
MaeIII GTNAC 5 cut(s) 148, 226, 367, 473, 1252
MalI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 678, 732
MbiI CCGCTC 1 cut(s) 312
MboI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
MflI RGATCY 4 cut(s) 142, 570, 629, 676
MhlI GDGCHC 2 cut(s) 119, 806
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 5 cut(s) 792, 899, 1101, 1158, 1343
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 2 cut(s) 61, 394
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 440
MseI TTAA 3 cut(s) 1050, 1161, 1323
MslI CAYNNNNRTG 2 cut(s) 80, 744
Msp20I TGGCCA 1 cut(s) 440
MspI CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
MspR9I CCNGG 2 cut(s) 196, 224
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 3 cut(s) 272, 398, 408
MwoI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 2 cut(s) 587, 1314
NdeII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
NlaIII CATG 8 cut(s) 79, 139, 394, 591, 743, 859, 1039, 1318
NlaIV GGNNCC 4 cut(s) 116, 769, 804, 1121
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 743
PagI TCATGA 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 1210
PceI AGGCCT 2 cut(s) 984, 1016
PciI ACATGT 1 cut(s) 739
PciSI GCTCTTC 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 1030
PdmI GAANNNNTTC 1 cut(s) 1271
PfeI GAWTC 5 cut(s) 97, 346, 387, 1239, 1307
Pfl23II CGTACG 1 cut(s) 360
PkrI GCNGC 5 cut(s) 397, 410, 509, 512, 585
PleI GAGTC 2 cut(s) 61, 394
PpsI GAGTC 2 cut(s) 61, 394
Ppu21I YACGTR 1 cut(s) 278
PscI ACATGT 1 cut(s) 739
Psp1406I AACGTT 2 cut(s) 248, 557
Psp6I CCWGG 1 cut(s) 194
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 194
PspLI CGTACG 1 cut(s) 360
PspN4I GGNNCC 4 cut(s) 116, 769, 804, 1121
PspOMI GGGCCC 1 cut(s) 802
PspPI GGNCC 4 cut(s) 130, 180, 802, 803
PstI CTGCAG 1 cut(s) 1118
PsuI RGATCY 4 cut(s) 142, 570, 629, 676
RsaI GTAC 2 cut(s) 362, 708
RsaNI GTAC 2 cut(s) 361, 707
RseI CAYNNNNRTG 2 cut(s) 80, 744
SalI GTCGAC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 941
SaqAI TTAA 3 cut(s) 1050, 1161, 1323
SatI GCNGC 5 cut(s) 396, 409, 508, 511, 584
Sau3AI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 730
Sau96I GGNCC 4 cut(s) 130, 180, 802, 803
SchI GAGTC 2 cut(s) 61, 394
ScrFI CCNGG 2 cut(s) 196, 224
SduI GDGCHC 2 cut(s) 119, 806
SfaNI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
SfcI CTRYAG 2 cut(s) 819, 1114
SinI GGWCC 2 cut(s) 130, 180
SmiMI CAYNNNNRTG 2 cut(s) 80, 744
SnaBI TACGTA 1 cut(s) 278
Sse9I AATT 5 cut(s) 792, 899, 1101, 1158, 1343
SseBI AGGCCT 2 cut(s) 984, 1016
SsiI CCGC 3 cut(s) 310, 396, 408
SspMI CTAG 4 cut(s) 71, 372, 807, 923
StuI AGGCCT 2 cut(s) 984, 1016
StyD4I CCNGG 2 cut(s) 194, 222
StyI CCWWGG 3 cut(s) 441, 587, 1314
TaaI ACNGT 1 cut(s) 1301
TaiI ACGT 3 cut(s) 251, 280, 560
TaqI TCGA 6 cut(s) 158, 189, 201, 252, 303, 528
TasI AATT 5 cut(s) 792, 899, 1101, 1158, 1343
TauI GCSGC 2 cut(s) 398, 411
TfiI GAWTC 5 cut(s) 97, 346, 387, 1239, 1307
Tru1I TTAA 3 cut(s) 1050, 1161, 1323
Tru9I TTAA 3 cut(s) 1050, 1161, 1323
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 507, 510, 583
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 152, 891, 1299
VpaK11BI GGWCC 2 cut(s) 130, 180
XagI CCTNNNNNAGG 1 cut(s) 175
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 5 cut(s) 238, 623, 778, 1042, 1130
XmiI GTMKAC 2 cut(s) 158, 1296
XmnI GAANNNNTTC 1 cut(s) 1271
XspI CTAG 4 cut(s) 71, 372, 807, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.