MD09G1266400.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001443
Physical Location & Seq
Forward (+)
34090350 .. 34090811
462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1266400.v1.1.491

Sequence Viewer

Length: 420 bp
ATGATTTCATCATTGTCAACTGCAGAAACCAGTGATAAATACATATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGCTACTAGAAACTCTTGTTGACGCAAAGAAAAACGTTTCGGAGACATCTTTGGGGGTTTTGGATGGTCTCTGTGATTGCAAACAAGGGAGAGAAAAGGCCCTCGAGAATGCTTTCACAATGCCTCTTTTGGTGAAGAAGATTTTGAGGATTTCGGAGTTGGGGACGGAGTTTTCAGTCTTTCTGATTGAGACGCTTCAAGTGGGCGTATTTCAGAAGCTCTTGGTGCTCTTGCAAGTCGGGGTTTATGATGGAATGAAGGGCAAGGTTATTGATCTGCTGAAATTGTTCAATCTGCACATTAGTAAATTGGACTGTGTGGATTCATCCATGAATTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

140

Amino Acids

15.37

Weight (kDa)

5.41

Isoelectric Point (pI)

28.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 7 - 136 3.4e-27 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 111
AcsI RAATTY 1 cut(s) 409
AgsI TTSAA 3 cut(s) 275, 367, 415
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 113, 149, 260
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 1 cut(s) 174
ApoI RAATTY 1 cut(s) 409
Asp700I GAANNNNTTC 2 cut(s) 188, 362
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 306
BccI CCATC 2 cut(s) 134, 320
BcoDI GTCTC 3 cut(s) 113, 149, 260
BfaI CTAG 1 cut(s) 83
BfmI CTRYAG 1 cut(s) 21
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 30
BseGI GGATG 2 cut(s) 145, 401
BseNI ACTGG 1 cut(s) 30
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 253
BsmAI GTCTC 3 cut(s) 113, 149, 260
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 253
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 1 cut(s) 349
BspANI GGCC 1 cut(s) 176
BspMAI CTGCAG 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 30
BssMI GATC 1 cut(s) 349
Bst4CI ACNGT 1 cut(s) 392
BstF5I GGATG 2 cut(s) 145, 401
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 3 cut(s) 113, 149, 260
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 21
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 2 cut(s) 145, 401
BtsIMutI CAGTG 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 2 cut(s) 107, 277
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 1 cut(s) 406
CviJI RGCY 2 cut(s) 176, 295
CviKI_1 RGCY 2 cut(s) 176, 295
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 1 cut(s) 409
FaiI YATR 3 cut(s) 44, 324, 407
FaqI GGGAC 1 cut(s) 253
FatI CATG 1 cut(s) 405
FokI GGATG 2 cut(s) 152, 388
FspBI CTAG 1 cut(s) 83
HaeIII GGCC 1 cut(s) 176
HgaI GACGC 2 cut(s) 107, 277
Hin1II CATG 1 cut(s) 409
HincII GTYRAC 2 cut(s) 18, 97
HindII GTYRAC 2 cut(s) 18, 97
HinfI GANTC 1 cut(s) 398
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 18, 97
Hpy188I TCNGA 4 cut(s) 118, 232, 261, 291
Hpy188III TCNNGA 2 cut(s) 48, 181
Hpy8I GTNNAC 2 cut(s) 18, 97
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 4 cut(s) 23, 156, 310, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 301
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 1 cut(s) 409
Kzo9I GATC 1 cut(s) 349
LpnPI CCDG 1 cut(s) 43
MaeI CTAG 1 cut(s) 83
MaeII ACGT 1 cut(s) 111
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 2 cut(s) 223, 226
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 4 cut(s) 59, 359, 383, 409
MnlI CCTC 3 cut(s) 188, 210, 216
MroXI GAANNNNTTC 2 cut(s) 188, 362
Mva1269I GAATGC 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 301
NdeII GATC 1 cut(s) 349
NlaIII CATG 1 cut(s) 409
PaeR7I CTCGAG 1 cut(s) 179
PctI GAATGC 1 cut(s) 190
PdmI GAANNNNTTC 2 cut(s) 188, 362
PfeI GAWTC 1 cut(s) 398
Psp1406I AACGTT 1 cut(s) 111
PspPI GGNCC 1 cut(s) 175
PstI CTGCAG 1 cut(s) 25
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 175
SduI GDGCHC 1 cut(s) 306
SetI ASST 3 cut(s) 114, 297, 345
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 179
SlaI CTCGAG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 4 cut(s) 59, 359, 383, 409
SspMI CTAG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 180
TasI AATT 4 cut(s) 59, 359, 383, 409
TfiI GAWTC 1 cut(s) 398
TscAI CASTG 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 347, 390
TspGWI ACGGA 1 cut(s) 257
TspRI CASTG 1 cut(s) 37
XapI RAATTY 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 2 cut(s) 188, 362
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.