MD17G1262600.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001443
Physical Location & Seq
Forward (+)
32311974 .. 32313442
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1262600.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGATCTTATCTTGGGGAAGAAGAAGGGCTGGACGTAAAGCCGACAACAAGAAACAATTTTGTAATTTGGACATGGACGTGGAGGAGATCGCGATTCCGACGCATTTCCGGTGCCCGATTTCCCTCGAGTTGATGAAAGATCCGGTGACGCTTTCGACGGGAATGACGTACGACAGGTCGAGCATTGAGATGTGGATCGAGACCGGCAACAGTACGTGTCCGGTGACTAATCAGGAACTAGTGAGTTTTGACCAGATTCCGAACCATGCACTGCGTCGGATGATCCAGGATTGGTGTGTGGAGAACAGGTCTAATGGCATCGAGCGGATTCCAACGCCGCGAATCCCCGTGACTCCCTATGAGGTTTTGAGGATCCGCGAGAGAGTTGCGGGGGCGGCAAGGCGTTTGGATGAGAAGAAGTGTCATGAACTGGTGGGGAAGATTAAAGCGTGGGGCAAGGAAAGCGAGCGGAACAAGAAGTGTATGGCCGAGAACGGAATTGGCAGCTGCTTATCCGCTGTGTTCGAGTCCTTTGCCGACCGTTCAATGGACGAACATGTTGAGATATTGGTGGAAATTTTATCAGTGCTGCCGTGGGTTTTTCCATTGAGTGAAGAAGGCCAAACAAAGCTTGGATCTTCAGGGTGTTTGCGATCTTTTGTGTCGTTTTTGGAGGGGAAAGATCTAACAGCACGGCAAAACGCAGTTTTGATACTTAGAAAGTTGTTTTCTTTGGATCAAAAATATGTGGATGCTTTCGGGGCGATTGAAGGAGGTACTGAAGCAATTGTTGGGATCATTCGACAACCTATTTGTCCTCAAGCGACTAAGGCTGCTCTAGCTTGTGTTCTTTTCATGATTTCATCACCGCCATCCGTGCAAACCAGTGACGAAATCAGATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGCTGCTGGAAATTCTTGTTGAGGCAGAGAAAAGCGTTTCGGAGAGAGCTTTGGGTGTTTTGGAGGTTCTTTGCGACTGCAAACAAGGGAGGGAAAAGGCCCTTGAGAATGCTCTCACAATGCCTCTTTTGGTCAAGAAGATTTTGAGGATTTCAGAGTTGGGGACGGAGTTTTCGGTGTCTATAATCTGGAAGCTGTGCAGGATTGAAACCGAGGAACGCGTTTTGATTGAGGCGCTTCAAGTGGGTGCTTTACAGAAGCTGTTGGTGGTCTTGCAGGTCGGGGTTTGTGATGGGATCAAGGACAAGATTACTGACTTGTTGAAATTGTTTAATCTGCACATGAGTAAATTGGACTGCGTTGATTCCTCCGTGGATTTCAAGTACCTGAAGAGGTCATTTTTGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

447

Amino Acids

50.03

Weight (kDa)

6.58

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 32 - 103 3.1e-21 U-box domain
ARM_PUB PF25598 121 - 439 2.3e-69 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1198
AccB1I GGYRCC 1 cut(s) 111
AccBSI CCGCTC 2 cut(s) 325, 469
AccII CGCG 4 cut(s) 92, 340, 378, 1152
AciI CCGC 8 cut(s) 325, 338, 376, 389, 395, 469, 516, 869
AclWI GGATC 9 cut(s) 134, 203, 277, 367, 380, 643, 744, 803, 1235
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 2 cut(s) 576, 942
AcuI CTGAAG 3 cut(s) 624, 801, 1340
AfaI GTAC 4 cut(s) 170, 214, 778, 1316
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 3 cut(s) 215, 556, 1150
AgsI TTSAA 6 cut(s) 546, 770, 1139, 1172, 1255, 1312
AhlI ACTAGT 1 cut(s) 238
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 6 cut(s) 507, 631, 842, 980, 1126, 1192
AluI AGCT 6 cut(s) 507, 631, 842, 980, 1126, 1192
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 9 cut(s) 134, 203, 277, 367, 380, 643, 744, 803, 1235
AlwNI CAGNNNCTG 1 cut(s) 1192
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 3 cut(s) 486, 619, 1029
ApeKI GCWGC 5 cut(s) 504, 507, 589, 833, 934
ApoI RAATTY 2 cut(s) 576, 942
ArsI GACNNNNNNTTYG 4 cut(s) 795, 827, 1087, 1119
AspLEI GCGC 1 cut(s) 1168
AspS9I GGNCC 1 cut(s) 1030
AsuHPI GGTGA 3 cut(s) 157, 235, 858
AvaI CYCGRG 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 116
BamHI GGATCC 1 cut(s) 372
BanI GGYRCC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 494, 516, 576, 820, 921
BccI CCATC 2 cut(s) 880, 1217
BceAI ACGGC 2 cut(s) 577, 710
BcgI CGANNNNNNTGC 4 cut(s) 368, 402, 515, 549
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 194
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 239, 839
BfoI RGCGCY 1 cut(s) 1169
BfuAI ACCTGC 1 cut(s) 1198
BglII AGATCT 1 cut(s) 682
BisI GCNGC 7 cut(s) 338, 396, 505, 508, 590, 834, 935
BlsI GCNGC 7 cut(s) 339, 397, 506, 509, 591, 835, 936
Bme1390I CCNGG 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 125
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 1030
BmiI GGNNCC 2 cut(s) 113, 374
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 2 cut(s) 327, 742
BpuEI CTTGAG 2 cut(s) 804, 1055
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 593, 1143, 1302
BsaWI WCCGGW 3 cut(s) 108, 142, 220
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 435, 885
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 593, 1143, 1302
BseGI GGATG 4 cut(s) 285, 415, 757, 872
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 1 cut(s) 547
BseNI ACTGG 2 cut(s) 435, 885
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 5 cut(s) 494, 516, 576, 820, 921
BsgI GTGCAG 2 cut(s) 1150, 1253
Bsh1236I CGCG 4 cut(s) 92, 340, 378, 1152
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 3 cut(s) 488, 621, 1031
BshNI GGYRCC 1 cut(s) 111
BsiEI CGRYCG 1 cut(s) 541
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 4 cut(s) 109, 143, 204, 221
BsiWI CGTACG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 1108
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 1108
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 3 cut(s) 488, 621, 1031
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 116
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 8 cut(s) 325, 338, 376, 389, 395, 469, 516, 869
BspANI GGCC 3 cut(s) 488, 621, 1031
BspFNI CGCG 4 cut(s) 92, 340, 378, 1152
BspHI TCATGA 2 cut(s) 424, 855
BspLI GGNNCC 2 cut(s) 113, 374
BspMI ACCTGC 1 cut(s) 1198
BspPI GGATC 9 cut(s) 134, 203, 277, 367, 380, 643, 744, 803, 1235
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 2 cut(s) 325, 469
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 435, 885
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 593, 1143, 1302
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 212, 542
Bst6I CTCTTC 1 cut(s) 1316
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 716, 828
BstDSI CCRYGG 2 cut(s) 593, 1302
BstF5I GGATG 4 cut(s) 285, 415, 757, 872
BstFNI CGCG 4 cut(s) 92, 340, 378, 1152
BstH2I RGCGCY 1 cut(s) 1169
BstHHI GCGC 1 cut(s) 1168
BstMAI GTCTC 1 cut(s) 194
BstMCI CGRYCG 1 cut(s) 541
BstMWI GCNNNNNNNGC 6 cut(s) 395, 462, 761, 830, 839, 877
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 1 cut(s) 285
BstSLI GKGCMC 1 cut(s) 116
BstUI CGCG 4 cut(s) 92, 340, 378, 1152
BstV1I GCAGC 5 cut(s) 494, 516, 576, 820, 921
BstX2I RGATCY 4 cut(s) 139, 372, 635, 682
BstYI RGATCY 4 cut(s) 139, 372, 635, 682
BsuRI GGCC 3 cut(s) 488, 621, 1031
BtgI CCRYGG 2 cut(s) 593, 1302
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 4 cut(s) 285, 415, 757, 872
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 3 cut(s) 269, 591, 892
BtuMI TCGCGA 1 cut(s) 92
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 1 cut(s) 467
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 2 cut(s) 424, 855
CfoI GCGC 1 cut(s) 1168
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 1030
CseI GACGC 3 cut(s) 109, 157, 263
Csp6I GTAC 4 cut(s) 169, 213, 777, 1315
CspCI CAANNNNNGTGG 2 cut(s) 891, 926
CviAII CATG 6 cut(s) 73, 266, 425, 557, 856, 1273
CviQI GTAC 4 cut(s) 169, 213, 777, 1315
DdeI CTNAG 2 cut(s) 716, 828
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 1316
EarI CTCTTC 1 cut(s) 1316
Eco31I GGTCTC 1 cut(s) 194
Eco57I CTGAAG 3 cut(s) 624, 801, 1340
Eco88I CYCGRG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 1030
EcoRII CCWGG 1 cut(s) 285
FaeI CATG 6 cut(s) 76, 269, 428, 560, 859, 1276
FaqI GGGAC 1 cut(s) 1108
FatI CATG 6 cut(s) 72, 265, 424, 556, 855, 1272
FauI CCCGC 1 cut(s) 382
Fnu4HI GCNGC 7 cut(s) 338, 396, 505, 508, 590, 834, 935
FokI GGATG 4 cut(s) 292, 422, 764, 859
Fsp4HI GCNGC 7 cut(s) 338, 396, 505, 508, 590, 834, 935
FspBI CTAG 2 cut(s) 239, 839
GlaI GCGC 1 cut(s) 1167
GluI GCNGC 7 cut(s) 338, 396, 505, 508, 590, 834, 935
HaeII RGCGCY 1 cut(s) 1169
HaeIII GGCC 3 cut(s) 488, 621, 1031
HapII CCGG 4 cut(s) 109, 143, 204, 221
HgaI GACGC 3 cut(s) 109, 157, 263
HhaI GCGC 1 cut(s) 1168
Hin1II CATG 6 cut(s) 76, 269, 428, 560, 859, 1276
Hin6I GCGC 1 cut(s) 1166
HinP1I GCGC 1 cut(s) 1166
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 7 cut(s) 94, 256, 328, 342, 352, 527, 1295
HpaII CCGG 4 cut(s) 109, 143, 204, 221
HphI GGTGA 3 cut(s) 157, 235, 858
Hpy188I TCNGA 6 cut(s) 99, 261, 279, 899, 973, 1087
Hpy188III TCNNGA 8 cut(s) 91, 199, 233, 425, 856, 903, 1066, 1120
Hpy99I CGWCG 3 cut(s) 103, 160, 279
HpyAV CCTTC 3 cut(s) 18, 611, 764
HpyCH4III ACNGT 2 cut(s) 212, 542
HpyCH4IV ACGT 4 cut(s) 34, 78, 167, 215
HpyCH4V TGCA 6 cut(s) 269, 880, 1011, 1131, 1207, 1270
HpyF10VI GCNNNNNNNGC 6 cut(s) 395, 462, 761, 830, 839, 877
HpyF3I CTNAG 2 cut(s) 716, 828
HpySE526I ACGT 4 cut(s) 34, 78, 167, 215
Hsp92II CATG 6 cut(s) 76, 269, 428, 560, 859, 1276
HspAI GCGC 1 cut(s) 1166
Lsp1109I GCAGC 5 cut(s) 494, 516, 576, 820, 921
LweI GCATC 2 cut(s) 327, 742
MaeI CTAG 2 cut(s) 239, 839
MaeII ACGT 4 cut(s) 34, 78, 167, 215
MaeIII GTNAC 4 cut(s) 145, 223, 349, 887
MbiI CCGCTC 2 cut(s) 325, 469
MboII GAAGA 8 cut(s) 30, 33, 427, 451, 626, 630, 1081, 1333
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 4 cut(s) 139, 372, 635, 682
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 9 cut(s) 56, 64, 498, 576, 786, 914, 942, 1256, 1280
MluI ACGCGT 1 cut(s) 1150
MlyI GAGTC 2 cut(s) 346, 536
MmeI TCCRAC 3 cut(s) 122, 257, 356
MseI TTAA 2 cut(s) 444, 1263
MslI CAYNNNNRTG 2 cut(s) 77, 188
MspA1I CMGCKG 2 cut(s) 507, 518
MspI CCGG 4 cut(s) 109, 143, 204, 221
MspR9I CCNGG 1 cut(s) 287
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 4 cut(s) 92, 340, 378, 1152
MwoI GCNNNNNNNGC 6 cut(s) 395, 462, 761, 830, 839, 877
NlaIII CATG 6 cut(s) 76, 269, 428, 560, 859, 1276
NlaIV GGNNCC 2 cut(s) 113, 374
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 4 cut(s) 145, 223, 349, 887
NruI TCGCGA 1 cut(s) 92
NspI RCATGY 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 125
PagI TCATGA 2 cut(s) 424, 855
PciI ACATGT 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 1 cut(s) 1045
PfeI GAWTC 5 cut(s) 94, 256, 328, 342, 1295
Pfl23II CGTACG 1 cut(s) 168
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 7 cut(s) 339, 397, 506, 509, 591, 835, 936
PleI GAGTC 2 cut(s) 346, 535
PpsI GAGTC 2 cut(s) 346, 535
Ppu21I YACGTR 1 cut(s) 216
PscI ACATGT 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspLI CGTACG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 113, 374
PspPI GGNCC 1 cut(s) 1030
PspXI VCTCGAGB 1 cut(s) 125
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 4 cut(s) 139, 372, 635, 682
PvuII CAGCTG 1 cut(s) 507
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 4 cut(s) 170, 214, 778, 1316
RsaNI GTAC 4 cut(s) 169, 213, 777, 1315
RseI CAYNNNNRTG 2 cut(s) 77, 188
SaqAI TTAA 2 cut(s) 444, 1263
SatI GCNGC 7 cut(s) 338, 396, 505, 508, 590, 834, 935
Sau96I GGNCC 1 cut(s) 1030
SchI GAGTC 2 cut(s) 346, 536
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 116
SfaNI GCATC 2 cut(s) 327, 742
Sfr274I CTCGAG 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 77, 188
SmlI CTYRAG 3 cut(s) 125, 819, 1034
SmoI CTYRAG 3 cut(s) 125, 819, 1034
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 9 cut(s) 56, 64, 498, 576, 786, 914, 942, 1256, 1280
SsiI CCGC 8 cut(s) 325, 338, 376, 389, 395, 469, 516, 869
SspMI CTAG 2 cut(s) 239, 839
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 212, 542
TaiI ACGT 4 cut(s) 37, 81, 170, 218
TaqI TCGA 7 cut(s) 126, 155, 179, 198, 321, 525, 802
TasI AATT 9 cut(s) 56, 64, 498, 576, 786, 914, 942, 1256, 1280
TauI GCSGC 2 cut(s) 340, 398
TfiI GAWTC 5 cut(s) 94, 256, 328, 342, 1295
Tru1I TTAA 2 cut(s) 444, 1263
Tru9I TTAA 2 cut(s) 444, 1263
TscAI CASTG 3 cut(s) 276, 591, 892
TseFI GTSAC 4 cut(s) 145, 223, 349, 887
TseI GCWGC 5 cut(s) 504, 507, 589, 833, 934
Tsp45I GTSAC 4 cut(s) 145, 223, 349, 887
TspDTI ATGAA 4 cut(s) 149, 441, 844, 852
TspGWI ACGGA 4 cut(s) 510, 865, 1112, 1291
TspRI CASTG 3 cut(s) 276, 591, 892
XapI RAATTY 2 cut(s) 576, 942
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 2 cut(s) 239, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.