Rmu_sc0000124.1_g000056

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000124.1
OG0001443
Physical Location & Seq
Reverse (-)
239360 .. 240700
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000124.1_g000056.1.cds

Sequence Viewer

Length: 1341 bp
atgactttgtcatggagaaggctgaggcccggccgtcactccgcgaaacattcacggcatggcgaggccggcggcgacattgagctgacgataccgagccaattcctgtgcccgatctcgttggacttgatgaaagatcccgtcacgttgtctaccgggatcacatacgatcggcagagcattgagaaatggatcgaagccggaaacgtgacttgccctactaccaaccaggtactcacaactttggagccgattccgaatcataccatgaggaaaatgatacaagactggtgcgtggagaagaagtccttcggcgtggagcgtatccccacgccacggattccggtgagttctgtggaagtcgccgagattctctcgaggattaggtcgtcggcggaaaagaaggacgaagccggctgcagagagctggtgcagaaggtcaaggcgttgactaaagaaagcgagcgcaacaagcggtgcattgtcgctaacggagcggcgagttctttagcggcggcgtttggtgctttttcaacggcgggttcttttcatgaaaacgtcggcgttttggaggagatattgtctgctctgacgttgattttcccgcttcgttcggaggccaacgtatatcttggatccacagattcgttgcattgcatggtgaggtttttggacggtggagatttgtcacagagaaggaacgcagtcttggttctaaaagaggctctttcagctgataagcaaaagactgatgctttggaagagattgaaggagcattggaagccctagtgaagctgatcaaagagccaatttgccctacttctactaaagcttcgttggtcatcatttaccacatgcttaattccacgtcttctaggtcaagagagagcgtcaaaggaaaatttgtgcaaatgggattggtgtccatgctatcggaattagttgtggatgccgagaggagcatttgcgaaaaggcgttgggggttctcgatgcgatttgtggaagcaagcaagggagggaagagacccgcgacaatgccctgatcatgccggtcgtagtcaagaagatgcttagggtttcggctttatctaccgagtttgctgtgtccattctttggaagctttgcaagaaggacgagagggaagacgacgagggaggtcttgttctagaggtgctccaagtgggagcttttcagaagctgttgttgctgttgcaagttgggtgtggggagaggactaaagagaaggcgaccgagttgttgaagttgttgaatcttcacagggcgaggttggagtgcattgagtccatggattttaaggagctcaagaggcccttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

446

Amino Acids

49.36

Weight (kDa)

8.18

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 1 cut(s) 152
AccII CGCG 2 cut(s) 44, 1032
AclWI GGATC 5 cut(s) 131, 167, 200, 630, 643
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 902
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 2 cut(s) 336, 1116
AgsI TTSAA 4 cut(s) 534, 770, 1264, 1273
AjiI CACGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
AluBI AGCT 9 cut(s) 85, 427, 734, 796, 833, 1123, 1190, 1201, 1324
AluI AGCT 9 cut(s) 85, 427, 734, 796, 833, 1123, 1190, 1201, 1324
Alw21I GWGCWC 2 cut(s) 1179, 1326
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 5 cut(s) 131, 167, 200, 630, 643
AlwNI CAGNNNCTG 1 cut(s) 1201
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 5 cut(s) 26, 31, 66, 618, 1331
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 739, 771
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 2 cut(s) 27, 1332
AsuC2I CCSGG 2 cut(s) 30, 157
AsuHPI GGTGA 2 cut(s) 358, 673
AvaI CYCGRG 1 cut(s) 376
BaeGI GKGCMC 1 cut(s) 113
BamHI GGATCC 1 cut(s) 635
BanII GRGCYC 1 cut(s) 1326
BbsI GAAGAC 2 cut(s) 864, 1152
Bbv12I GWGCWC 2 cut(s) 1179, 1326
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 404
BceAI ACGGC 3 cut(s) 18, 71, 552
BciT130I CCWGG 1 cut(s) 230
BciVI GTATCC 1 cut(s) 335
BclI TGATCA 2 cut(s) 798, 1044
BcnI CCSGG 2 cut(s) 30, 157
BcoDI GTCTC 1 cut(s) 1019
BfaI CTAG 3 cut(s) 788, 876, 1169
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 335
BisI GCNGC 5 cut(s) 73, 418, 498, 513, 516
BlsI GCNGC 5 cut(s) 74, 419, 499, 514, 517
Bme1390I CCNGG 3 cut(s) 30, 157, 230
BmeT110I CYCGRG 1 cut(s) 376
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 2 cut(s) 27, 1332
BmiI GGNNCC 2 cut(s) 249, 637
BmrFI CCNGG 3 cut(s) 30, 157, 230
BmsI GCATC 4 cut(s) 742, 940, 982, 1059
BpiI GAAGAC 2 cut(s) 864, 1152
BplI GAGNNNNNCTC 2 cut(s) 359, 391
Bpu10I CCTNAGC 2 cut(s) 23, 1073
BpuEI CTTGAG 1 cut(s) 1310
BpuMI CCSGG 2 cut(s) 30, 157
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 2 cut(s) 335, 1308
BsaWI WCCGGW 1 cut(s) 343
BsaXI ACNNNNNCTCC 6 cut(s) 290, 320, 566, 596, 1149, 1179
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 1116
Bse118I RCCGGY 3 cut(s) 68, 413, 1051
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 652
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 335, 1308
BseGI GGATG 1 cut(s) 955
BseLI CCNNNNNNNGG 2 cut(s) 336, 1116
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 587, 973
BseSI GKGCMC 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 2 cut(s) 44, 1032
Bsh1285I CGRYCG 4 cut(s) 34, 172, 1056, 1254
BshFI GGCC 5 cut(s) 28, 33, 68, 620, 1333
BsiEI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BsiHKAI GWGCWC 2 cut(s) 1179, 1326
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 7 cut(s) 30, 69, 156, 201, 344, 414, 1052
BslI CCNNNNNNNGG 2 cut(s) 336, 1116
BsmAI GTCTC 1 cut(s) 1019
BsnI GGCC 5 cut(s) 28, 33, 68, 620, 1333
Bso31I GGTCTC 1 cut(s) 1019
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 3 cut(s) 113, 1179, 1326
Bsp143I GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
Bsp19I CCATGG 1 cut(s) 1308
BspANI GGCC 5 cut(s) 28, 33, 68, 620, 1333
BspCNI CTCAG 1 cut(s) 15
BspFNI CGCG 2 cut(s) 44, 1032
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 249, 637
BspMAI CTGCAG 1 cut(s) 422
BspPI GGATC 5 cut(s) 131, 167, 200, 630, 643
BspTNI GGTCTC 1 cut(s) 1019
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 652
BsrFI RCCGGY 3 cut(s) 68, 413, 1051
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 3 cut(s) 68, 413, 1051
BssECI CCNNGG 2 cut(s) 335, 1308
BssMI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
BssT1I CCWWGG 1 cut(s) 1308
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 677
Bst6I CTCTTC 2 cut(s) 756, 1017
BstC8I GCNNGC 4 cut(s) 70, 415, 464, 1010
BstDEI CTNAG 2 cut(s) 23, 1073
BstDSI CCRYGG 2 cut(s) 335, 1308
BstF5I GGATG 1 cut(s) 955
BstFNI CGCG 2 cut(s) 44, 1032
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 8 cut(s) 117, 139, 162, 172, 195, 638, 801, 1047
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
BstMCI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BstMWI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 3 cut(s) 28, 155, 228
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 2 cut(s) 44, 1032
BstV1I GCAGC 1 cut(s) 404
BstV2I GAAGAC 2 cut(s) 864, 1152
BstX2I RGATCY 2 cut(s) 136, 635
BstYI RGATCY 2 cut(s) 136, 635
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 5 cut(s) 28, 33, 68, 620, 1333
BtgI CCRYGG 2 cut(s) 335, 1308
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 1 cut(s) 955
Cac8I GCNNGC 4 cut(s) 70, 415, 464, 1010
CaiI CAGNNNCTG 1 cut(s) 1201
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 468
Cfr10I RCCGGY 3 cut(s) 68, 413, 1051
Cfr13I GGNCC 2 cut(s) 27, 1332
CseI GACGC 1 cut(s) 880
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 233
CviAII CATG 9 cut(s) 12, 59, 268, 551, 658, 856, 928, 1048, 1309
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 2 cut(s) 23, 1073
DpnI GATC 8 cut(s) 116, 138, 161, 171, 194, 637, 800, 1046
DpnII GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 756, 1017
EarI CTCTTC 2 cut(s) 756, 1017
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 1324
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 1308
Eco24I GRGCYC 1 cut(s) 1326
Eco31I GGTCTC 1 cut(s) 1019
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 1324
Eco88I CYCGRG 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 1324
EcoO109I RGGNCCY 1 cut(s) 1332
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 1308
EcoT38I GRGCYC 1 cut(s) 1326
ErhI CCWWGG 1 cut(s) 1308
FaeI CATG 9 cut(s) 15, 62, 271, 554, 661, 859, 931, 1051, 1312
FalI AAGNNNNNCTT 5 cut(s) 293, 325, 711, 743, 1319
FatI CATG 9 cut(s) 11, 58, 267, 550, 657, 855, 927, 1047, 1308
FauI CCCGC 3 cut(s) 532, 612, 1037
FbaI TGATCA 2 cut(s) 798, 1044
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 73, 418, 498, 513, 516
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 1 cut(s) 1326
Fsp4HI GCNGC 5 cut(s) 73, 418, 498, 513, 516
FspBI CTAG 3 cut(s) 788, 876, 1169
GlaI GCGC 1 cut(s) 467
GluI GCNGC 5 cut(s) 73, 418, 498, 513, 516
HaeIII GGCC 5 cut(s) 28, 33, 68, 620, 1333
HapII CCGG 7 cut(s) 30, 69, 156, 201, 344, 414, 1052
HgaI GACGC 1 cut(s) 880
HhaI GCGC 1 cut(s) 468
Hin1II CATG 9 cut(s) 15, 62, 271, 554, 661, 859, 931, 1051, 1312
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 2 cut(s) 831, 1121
HinfI GANTC 7 cut(s) 253, 259, 340, 370, 644, 1273, 1304
HpaII CCGG 7 cut(s) 30, 69, 156, 201, 344, 414, 1052
HphI GGTGA 2 cut(s) 358, 673
Hpy166II GTNNAC 2 cut(s) 153, 450
Hpy188I TCNGA 5 cut(s) 258, 591, 616, 937, 1197
Hpy188III TCNNGA 7 cut(s) 376, 551, 882, 989, 1063, 1169, 1327
Hpy8I GTNNAC 2 cut(s) 153, 450
Hpy99I CGWCG 3 cut(s) 394, 563, 1154
HpyAV CCTTC 8 cut(s) 12, 319, 397, 430, 690, 764, 1126, 1240
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 6 cut(s) 146, 207, 558, 593, 624, 869
HpyCH4V TGCA 9 cut(s) 420, 433, 480, 652, 657, 910, 1128, 1216, 1299
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
HpyF3I CTNAG 2 cut(s) 23, 1073
HpySE526I ACGT 6 cut(s) 146, 207, 558, 593, 624, 869
Hsp92II CATG 9 cut(s) 15, 62, 271, 554, 661, 859, 931, 1051, 1312
HspAI GCGC 1 cut(s) 466
KroI GCCGGC 2 cut(s) 68, 413
KroNI GCCGGC 2 cut(s) 70, 415
Ksp22I TGATCA 2 cut(s) 798, 1044
Kzo9I GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
LmnI GCTCC 8 cut(s) 247, 319, 494, 773, 960, 1182, 1187, 1321
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 4 cut(s) 742, 940, 982, 1059
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 3 cut(s) 788, 876, 1169
MaeII ACGT 6 cut(s) 146, 207, 558, 593, 624, 869
MaeIII GTNAC 4 cut(s) 35, 142, 208, 687
MalI GATC 8 cut(s) 116, 138, 161, 171, 194, 637, 800, 1046
MbiI CCGCTC 1 cut(s) 497
MboI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
MboII GAAGA 7 cut(s) 313, 773, 864, 1034, 1078, 1157, 1268
MflI RGATCY 2 cut(s) 136, 635
MhlI GDGCHC 3 cut(s) 113, 1179, 1326
MluCI AATT 5 cut(s) 101, 810, 862, 902, 938
MlyI GAGTC 1 cut(s) 1313
MmeI TCCRAC 2 cut(s) 102, 1272
MroNI GCCGGC 2 cut(s) 68, 413
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 2 cut(s) 861, 1317
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 7 cut(s) 30, 69, 156, 201, 344, 414, 1052
MspR9I CCNGG 3 cut(s) 30, 157, 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 2 cut(s) 44, 1032
MwoI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
NaeI GCCGGC 2 cut(s) 70, 415
NciI CCSGG 2 cut(s) 30, 157
NcoI CCATGG 1 cut(s) 1308
NdeII GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
NgoMIV GCCGGC 2 cut(s) 68, 413
NlaIII CATG 9 cut(s) 15, 62, 271, 554, 661, 859, 931, 1051, 1312
NlaIV GGNNCC 2 cut(s) 249, 637
NmeAIII GCCGAG 2 cut(s) 391, 979
NmuCI GTSAC 4 cut(s) 35, 142, 208, 687
NspI RCATGY 1 cut(s) 859
PaeR7I CTCGAG 1 cut(s) 376
PagI TCATGA 1 cut(s) 550
PdiI GCCGGC 2 cut(s) 70, 415
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 6 cut(s) 253, 259, 340, 370, 644, 1273
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 1116
PkrI GCNGC 5 cut(s) 74, 419, 499, 514, 517
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 1 cut(s) 1312
PpsI GAGTC 1 cut(s) 1312
Psp124BI GAGCTC 1 cut(s) 1326
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 249, 637
PspPI GGNCC 2 cut(s) 27, 1332
PstI CTGCAG 1 cut(s) 422
PstNI CAGNNNCTG 1 cut(s) 1201
PsuI RGATCY 2 cut(s) 136, 635
PsyI GACNNNGTC 1 cut(s) 7
PvuI CGATCG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SacI GAGCTC 1 cut(s) 1326
SaqAI TTAA 2 cut(s) 861, 1317
SatI GCNGC 5 cut(s) 73, 418, 498, 513, 516
Sau3AI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
Sau96I GGNCC 2 cut(s) 27, 1332
SchI GAGTC 1 cut(s) 1313
ScrFI CCNGG 3 cut(s) 30, 157, 230
SduI GDGCHC 3 cut(s) 113, 1179, 1326
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 4 cut(s) 742, 940, 982, 1059
SfcI CTRYAG 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 376
SmlI CTYRAG 2 cut(s) 376, 1325
SmoI CTYRAG 2 cut(s) 376, 1325
Sse9I AATT 5 cut(s) 101, 810, 862, 902, 938
SspMI CTAG 3 cut(s) 788, 876, 1169
SstI GAGCTC 1 cut(s) 1326
StyD4I CCNGG 3 cut(s) 28, 155, 228
StyI CCWWGG 1 cut(s) 1308
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 6 cut(s) 149, 210, 561, 596, 627, 872
TaqI TCGA 3 cut(s) 195, 377, 990
TaqII GACCGA 1 cut(s) 1268
TasI AATT 5 cut(s) 101, 810, 862, 902, 938
TauI GCSGC 4 cut(s) 75, 500, 515, 518
TfiI GAWTC 6 cut(s) 253, 259, 340, 370, 644, 1273
Tru1I TTAA 2 cut(s) 861, 1317
Tru9I TTAA 2 cut(s) 861, 1317
TseFI GTSAC 4 cut(s) 35, 142, 208, 687
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 4 cut(s) 35, 142, 208, 687
TspDTI ATGAA 3 cut(s) 146, 539, 567
TspGWI ACGGA 2 cut(s) 352, 507
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 1116
XapI RAATTY 1 cut(s) 902
XbaI TCTAGA 1 cut(s) 1168
XceI RCATGY 1 cut(s) 859
XhoI CTCGAG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 3 cut(s) 788, 876, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.