MD16G1015400.v1.1

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001443
Physical Location & Seq
Forward (+)
1190620 .. 1191948
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1015400.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGACCTTGTCATGGAGAAGATTGAGAGCCGGCCGCCGTGCTGCCAAGGAAGGTGAGACTGGCGACATGGAGCTGACCATACCAAACCACTTCCGGTGCCCAATCTCACTCGACTTGATGAAGGATCCGGTCACGCTGTCCACCGGGATCACGTACGACCGCCACAGCATAGAGACGTGGATCGAAGCAGAAAACTTCACATGCCCTATTACCAACCAAGTCCTCACAAGCTTTGATCTCATTCCCAACCACACCATCCGCAAGATGATACAAGATTGGTGCGTCGAGAAAAAGTCTTTTGGAATCGAGCGCATTCCCACCCCTCGCATTCCGATCAGTTCGGTTCAGGTTGCCGAGATTTTATCAAAGATCTCGACATCGAGTCATCATCAGAACAAGGATGATTGCCAAGTTTTGGTGACGAAGATCAAGGCGTTGGCGAAAGAAAGCGAGCGTAACAAGCGTTGCATTGTGGCTACTGGCACGGGGAGCGTCTTAGCGGGTGCGTTTAGTGCACTTTCAAATTCTCAACACCATCAAAACGTTGCCGTTTTGGAGGAGATTTTGTCTGCTTTGACTTTGGTTTTCCCGCTTGACGGGGAGGCCAGTTTGTACCTCGGATCGCCTGCTTCGTTGCGTTGCATGGTGTGGTTTTTGGAGAGTGGAGATTTGTCGCAGAGGCGAAACGCAGCGTTAGTGCTCAAAGAGACCGCTGTCTCATCCGATCATGATCATCAGAAGACCGATGCTTTGGCAGAGATTGAAGGAGCTCTCGAAGCGCTAGTGAAGCTGATCAAAGAACCAGTTTGCGCTACTTCTACAAAAGCGTCCTTGGTCATCATTTATCAAATGGTTAACTCATCATCGTCTCTTTCGAAAGAGAAAATTAAAGAGAGATTTGTGGAGATGGAATTGGTGTCGCTGCTTTCGGACTTTCTTGTAGATGCCGAAAGAAGTATTTGCGAGAGGGCATTGGGAGTTCTTGACAGACTTTGTGGAAGCAAGCAAGGAAGGGAAAAGGCCTACCTTCATGCCTTGACCATGCCGGTTGTGGTGAAAAAGATACTTAGGGTTTCAGATCTGGCCACAGAGTTTTCTGTGTCTATTCTTTGGAAGCTTTGCAAGAACGAGACCAGGGAAGATGGAGGTGTGGTCGTGGAGTCCCTTCAAGTGGGTGCGTTTCAGAAGCTCTTGCTGCTTTTGCAAGTTGGGTGTGCGGAGAGGACCAAAGAAAAGGCCACGGACTTGTTGAAGGCGTTGAACATTCATAGGGAGAGGTTGGAGTGCATTGATTCTATGGATTTTAAGCAGCTCAAGAGGTCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

443

Amino Acids

49.25

Weight (kDa)

7.55

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 27 - 98 6e-22 U-box domain
ARM_PUB PF25598 116 - 437 4.1e-69 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 415
AciI CCGC 7 cut(s) 34, 160, 259, 500, 590, 711, 1217
AclI AACGTT 1 cut(s) 543
AclWI GGATC 5 cut(s) 119, 132, 155, 188, 628
AcoI YGGCCR 2 cut(s) 31, 1083
AcsI RAATTY 1 cut(s) 523
AfaI GTAC 2 cut(s) 155, 614
AfeI AGCGCT 1 cut(s) 780
AfiI CCNNNNNNNGG 4 cut(s) 12, 415, 596, 1171
AgsI TTSAA 5 cut(s) 522, 764, 1169, 1252, 1261
AhdI GACNNNNNGTC 1 cut(s) 381
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 1 cut(s) 1133
AluBI AGCT 7 cut(s) 73, 231, 770, 790, 1117, 1189, 1312
AluI AGCT 7 cut(s) 73, 231, 770, 790, 1117, 1189, 1312
Alw21I GWGCWC 3 cut(s) 517, 702, 772
Alw26I GTCTC 6 cut(s) 50, 167, 701, 721, 873, 1124
Alw44I GTGCAC 1 cut(s) 513
AlwI GGATC 5 cut(s) 119, 132, 155, 188, 628
Aor51HI AGCGCT 1 cut(s) 780
AoxI GGCC 5 cut(s) 31, 603, 1020, 1083, 1236
ApaLI GTGCAC 1 cut(s) 513
ApeKI GCWGC 5 cut(s) 41, 689, 922, 1195, 1309
ApoI RAATTY 1 cut(s) 523
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AspLEI GCGC 3 cut(s) 312, 781, 812
AspS9I GGNCC 1 cut(s) 1224
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 65, 430, 1066
AsuII TTCGAA 1 cut(s) 875
AvaII GGWCC 1 cut(s) 1224
BaeGI GKGCMC 2 cut(s) 101, 517
BalI TGGCCA 1 cut(s) 1085
BamHI GGATCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 772
BbsI GAAGAC 1 cut(s) 746
Bbv12I GWGCWC 3 cut(s) 517, 702, 772
BbvI GCAGC 5 cut(s) 28, 701, 909, 1182, 1321
BccI CCATC 4 cut(s) 263, 543, 901, 1136
BceAI ACGGC 2 cut(s) 21, 533
BciT130I CCWGG 1 cut(s) 1135
BclI TGATCA 2 cut(s) 730, 792
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 6 cut(s) 50, 167, 701, 721, 873, 1124
BfaI CTAG 1 cut(s) 782
BfoI RGCGCY 1 cut(s) 782
BglII AGATCT 2 cut(s) 369, 1078
BisI GCNGC 6 cut(s) 34, 42, 690, 923, 1196, 1310
BlsI GCNGC 6 cut(s) 35, 43, 691, 924, 1197, 1311
Bme1390I CCNGG 2 cut(s) 145, 1135
Bme18I GGWCC 1 cut(s) 1224
BmeRI GACNNNNNGTC 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 1224
BmiI GGNNCC 2 cut(s) 98, 126
BmrFI CCNGG 2 cut(s) 145, 1135
BmsI GCATC 2 cut(s) 736, 934
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 746
Bpu14I TTCGAA 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 1298
BpuMI CCSGG 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 729
BsaI GGTCTC 2 cut(s) 701, 1124
BsaJI CCNNGG 5 cut(s) 45, 616, 831, 1134, 1239
BsaWI WCCGGW 2 cut(s) 93, 127
BsaXI ACNNNNNCTCC 4 cut(s) 551, 581, 1137, 1167
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 415, 596, 1171
Bse118I RCCGGY 2 cut(s) 29, 1045
Bse1I ACTGG 4 cut(s) 64, 484, 606, 803
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 1 cut(s) 1135
BseDI CCNNGG 5 cut(s) 45, 616, 831, 1134, 1239
BseGI GGATG 3 cut(s) 255, 406, 719
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 4 cut(s) 12, 415, 596, 1171
BseNI ACTGG 4 cut(s) 64, 484, 606, 803
BseRI GAGGAG 1 cut(s) 572
BseSI GKGCMC 2 cut(s) 101, 517
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 5 cut(s) 28, 701, 909, 1182, 1321
Bsh1285I CGRYCG 2 cut(s) 34, 160
BshFI GGCC 5 cut(s) 33, 605, 1022, 1085, 1238
BshNI GGYRCC 1 cut(s) 96
BsiEI CGRYCG 2 cut(s) 34, 160
BsiHKAI GWGCWC 3 cut(s) 517, 702, 772
BsiSI CCGG 5 cut(s) 30, 94, 128, 144, 1046
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 4 cut(s) 12, 415, 596, 1171
BsmAI GTCTC 6 cut(s) 50, 167, 701, 721, 873, 1124
BsmBI CGTCTC 2 cut(s) 167, 873
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 2 cut(s) 312, 327
BsnI GGCC 5 cut(s) 33, 605, 1022, 1085, 1238
Bso31I GGTCTC 2 cut(s) 701, 1124
Bsp119I TTCGAA 1 cut(s) 875
Bsp1286I GDGCHC 4 cut(s) 101, 517, 702, 772
BspACI CCGC 7 cut(s) 34, 160, 259, 500, 590, 711, 1217
BspANI GGCC 5 cut(s) 33, 605, 1022, 1085, 1238
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 2 cut(s) 98, 126
BspPI GGATC 5 cut(s) 119, 132, 155, 188, 628
BspT104I TTCGAA 1 cut(s) 875
BspT107I GGYRCC 1 cut(s) 96
BspTNI GGTCTC 2 cut(s) 701, 1124
BsrFI RCCGGY 2 cut(s) 29, 1045
BsrI ACTGG 4 cut(s) 64, 484, 606, 803
BssAI RCCGGY 2 cut(s) 29, 1045
BssECI CCNNGG 5 cut(s) 45, 616, 831, 1134, 1239
BssT1I CCWWGG 2 cut(s) 45, 831
Bst2UI CCWGG 1 cut(s) 1135
BstBAI YACGTR 1 cut(s) 153
BstBI TTCGAA 1 cut(s) 875
BstC8I GCNNGC 4 cut(s) 31, 452, 627, 1004
BstDEI CTNAG 2 cut(s) 496, 1067
BstDSI CCRYGG 1 cut(s) 1239
BstF5I GGATG 3 cut(s) 255, 406, 719
BstH2I RGCGCY 1 cut(s) 782
BstHHI GCGC 3 cut(s) 312, 781, 812
BstMAI GTCTC 6 cut(s) 50, 167, 701, 721, 873, 1124
BstMCI CGRYCG 2 cut(s) 34, 160
BstMWI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 776, 787, 1195, 1201
BstNI CCWGG 1 cut(s) 1135
BstNSI RCATGY 1 cut(s) 204
BstPAI GACNNNNGTC 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 143, 1133
BstSLI GKGCMC 2 cut(s) 101, 517
BstV1I GCAGC 5 cut(s) 28, 701, 909, 1182, 1321
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 3 cut(s) 124, 369, 1078
BstYI RGATCY 3 cut(s) 124, 369, 1078
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 5 cut(s) 33, 605, 1022, 1085, 1238
BtgI CCRYGG 1 cut(s) 1239
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 255, 406, 719
Cac8I GCNNGC 4 cut(s) 31, 452, 627, 1004
CciI TCATGA 1 cut(s) 727
CfoI GCGC 3 cut(s) 312, 781, 812
Cfr10I RCCGGY 2 cut(s) 29, 1045
Cfr13I GGNCC 1 cut(s) 1224
CseI GACGC 3 cut(s) 271, 481, 816
Csp6I GTAC 2 cut(s) 154, 613
CviAII CATG 7 cut(s) 12, 67, 201, 643, 728, 1031, 1042
CviQI GTAC 2 cut(s) 154, 613
DdeI CTNAG 2 cut(s) 496, 1067
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 2 cut(s) 31, 1083
EagI CGGCCG 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 770
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 45, 831
Eco147I AGGCCT 1 cut(s) 1022
Eco24I GRGCYC 1 cut(s) 772
Eco31I GGTCTC 2 cut(s) 701, 1124
Eco47I GGWCC 1 cut(s) 1224
Eco47III AGCGCT 1 cut(s) 780
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 1133
EcoT14I CCWWGG 2 cut(s) 45, 831
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 45, 831
Esp3I CGTCTC 2 cut(s) 167, 873
FaeI CATG 7 cut(s) 15, 70, 204, 646, 731, 1034, 1045
FaqI GGGAC 1 cut(s) 1147
FatI CATG 7 cut(s) 11, 66, 200, 642, 727, 1030, 1041
FauI CCCGC 2 cut(s) 493, 597
FbaI TGATCA 2 cut(s) 730, 792
Fnu4HI GCNGC 6 cut(s) 34, 42, 690, 923, 1196, 1310
FokI GGATG 3 cut(s) 242, 413, 706
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 6 cut(s) 34, 42, 690, 923, 1196, 1310
FspBI CTAG 1 cut(s) 782
GlaI GCGC 3 cut(s) 311, 780, 811
GluI GCNGC 6 cut(s) 34, 42, 690, 923, 1196, 1310
HaeII RGCGCY 1 cut(s) 782
HaeIII GGCC 5 cut(s) 33, 605, 1022, 1085, 1238
HapII CCGG 5 cut(s) 30, 94, 128, 144, 1046
HgaI GACGC 3 cut(s) 271, 481, 816
HhaI GCGC 3 cut(s) 312, 781, 812
Hin1II CATG 7 cut(s) 15, 70, 204, 646, 731, 1034, 1045
Hin6I GCGC 3 cut(s) 310, 779, 810
HinP1I GCGC 3 cut(s) 310, 779, 810
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 2 cut(s) 229, 1115
HinfI GANTC 4 cut(s) 303, 382, 1160, 1292
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 5 cut(s) 30, 94, 128, 144, 1046
HphI GGTGA 3 cut(s) 65, 430, 1066
Hpy166II GTNNAC 3 cut(s) 141, 515, 856
Hpy188I TCNGA 8 cut(s) 333, 393, 620, 724, 738, 931, 1078, 1185
Hpy188III TCNNGA 6 cut(s) 286, 373, 728, 773, 983, 1315
Hpy8I GTNNAC 3 cut(s) 141, 515, 856
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 7 cut(s) 44, 115, 758, 1005, 1037, 1175, 1246
HpyCH4IV ACGT 3 cut(s) 152, 176, 543
HpyCH4V TGCA 6 cut(s) 468, 515, 642, 1122, 1204, 1287
HpyF10VI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 776, 787, 1195, 1201
HpyF3I CTNAG 2 cut(s) 496, 1067
HpySE526I ACGT 3 cut(s) 152, 176, 543
Hsp92II CATG 7 cut(s) 15, 70, 204, 646, 731, 1034, 1045
HspAI GCGC 3 cut(s) 310, 779, 810
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 2 cut(s) 730, 792
KspAI GTTAAC 1 cut(s) 856
LmnI GCTCC 3 cut(s) 70, 489, 767
Lsp1109I GCAGC 5 cut(s) 28, 701, 909, 1182, 1321
LweI GCATC 2 cut(s) 736, 934
MaeI CTAG 1 cut(s) 782
MaeII ACGT 3 cut(s) 152, 176, 543
MaeIII GTNAC 3 cut(s) 130, 418, 455
MboII GAAGA 4 cut(s) 30, 436, 751, 1151
MflI RGATCY 3 cut(s) 124, 369, 1078
MhlI GDGCHC 4 cut(s) 101, 517, 702, 772
MlsI TGGCCA 1 cut(s) 1085
MluCI AATT 3 cut(s) 523, 885, 911
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 2 cut(s) 391, 1169
MmeI TCCRAC 1 cut(s) 1260
Mox20I TGGCCA 1 cut(s) 1085
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 1085
MseI TTAA 3 cut(s) 855, 888, 1305
Msp20I TGGCCA 1 cut(s) 1085
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 5 cut(s) 30, 94, 128, 144, 1046
MspR9I CCNGG 2 cut(s) 145, 1135
Mva1269I GAATGC 2 cut(s) 312, 327
MvaI CCWGG 1 cut(s) 1135
MwoI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 776, 787, 1195, 1201
NaeI GCCGGC 1 cut(s) 31
NciI CCSGG 1 cut(s) 145
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 7 cut(s) 15, 70, 204, 646, 731, 1034, 1045
NlaIV GGNNCC 2 cut(s) 98, 126
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 2 cut(s) 130, 418
NspI RCATGY 1 cut(s) 204
NspV TTCGAA 1 cut(s) 875
PagI TCATGA 1 cut(s) 727
PceI AGGCCT 1 cut(s) 1022
PcsI WCGNNNNNNNCGW 2 cut(s) 629, 872
PctI GAATGC 2 cut(s) 312, 327
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 303, 1292
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 6 cut(s) 35, 43, 691, 924, 1197, 1311
PleI GAGTC 2 cut(s) 390, 1168
PpsI GAGTC 2 cut(s) 390, 1168
Ppu21I YACGTR 1 cut(s) 153
PshAI GACNNNNGTC 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 772
Psp1406I AACGTT 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 1133
PspGI CCWGG 1 cut(s) 1133
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 98, 126
PspPI GGNCC 1 cut(s) 1224
PsuI RGATCY 3 cut(s) 124, 369, 1078
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 155, 614
RsaNI GTAC 2 cut(s) 154, 613
SacI GAGCTC 1 cut(s) 772
SaqAI TTAA 3 cut(s) 855, 888, 1305
SatI GCNGC 6 cut(s) 34, 42, 690, 923, 1196, 1310
Sau96I GGNCC 1 cut(s) 1224
SchI GAGTC 2 cut(s) 391, 1169
ScrFI CCNGG 2 cut(s) 145, 1135
SduI GDGCHC 4 cut(s) 101, 517, 702, 772
SfaNI GCATC 2 cut(s) 736, 934
SfuI TTCGAA 1 cut(s) 875
SinI GGWCC 1 cut(s) 1224
SmlI CTYRAG 1 cut(s) 1313
SmoI CTYRAG 1 cut(s) 1313
Sse9I AATT 3 cut(s) 523, 885, 911
SseBI AGGCCT 1 cut(s) 1022
SsiI CCGC 7 cut(s) 34, 160, 259, 500, 590, 711, 1217
SspMI CTAG 1 cut(s) 782
SstI GAGCTC 1 cut(s) 772
StuI AGGCCT 1 cut(s) 1022
StyD4I CCNGG 2 cut(s) 143, 1133
StyI CCWWGG 2 cut(s) 45, 831
TaiI ACGT 3 cut(s) 155, 179, 546
TaqI TCGA 8 cut(s) 111, 183, 285, 306, 374, 380, 774, 875
TaqII GACCGA 1 cut(s) 758
TasI AATT 3 cut(s) 523, 885, 911
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 303, 1292
Tru1I TTAA 3 cut(s) 855, 888, 1305
Tru9I TTAA 3 cut(s) 855, 888, 1305
TseFI GTSAC 2 cut(s) 130, 418
TseI GCWGC 5 cut(s) 41, 689, 922, 1195, 1309
Tsp45I GTSAC 2 cut(s) 130, 418
TspDTI ATGAA 3 cut(s) 134, 1019, 1256
TspGWI ACGGA 1 cut(s) 1256
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 415
VneI GTGCAC 1 cut(s) 513
VpaK11BI GGWCC 1 cut(s) 1224
XapI RAATTY 1 cut(s) 523
XceI RCATGY 1 cut(s) 204
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XspI CTAG 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.