pycom13g01490

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001443
Physical Location & Seq
Forward (+)
963238 .. 964569
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01490.1

Sequence Viewer

Length: 1332 bp
ATGACCTTGTCATGGAGAAGGCTGAGAGCCGGCCGCGGTGCTGCCAAGGAGGGAGAGGCCGGCGACATGGAGCTGACCATACCAAACCATTTCCGGTGCCCAATTTCACTTGAGTTGATGAAGGATCCGGTCACGCTGTCCACCGGGATCACGTACGACCGTCAGAGCATAGAGACGTGGATTGAAGCCGGCAACTTCACGTGCCCTATAACAAACCAAGTCCTCACAAGCTTCGATCCAATTCCCAATCACACCATCCGCAAGATGATACAGGGTTGGTGCGTCGAGAAAAAGTCTTTTGGAATCGAGCGCATTCCCACCCCTCGCATTCCGATCAGTTCGGTTCAGGTGACCGAGATTTTATCAAAGATTACGACATCGAGTCATCATGAGAACAAGGATGATTGCCAAGTTTTGGTGGCGAAGATCAAGGAGTTGGCGAAAGAAAGCGAGCGTAACAAGCGTTGCATTGTGGCTAGTGGCACGGGGAGCGTTCTAGCGGGTGCGTTTAATGCATTTTCAACTTCTCAACACCATCAAAACGTTGCCGTTTTGGAGGAGATTTTGTCTGCTTTGACTTTGGTTTTCCCGCTTGACAAGGAGGCTAACTTGTACCTCGGATCGCCTTCTTCGTTGTGTTGCATGGTGTGGTTTTTGGAGAGTGGAGATTTCTCACAAAGACGAAACGCATCGTTGGTGCTCAAAGAGACCGCTGTCTCATCTGATCATGATCATCAGAAGATCGATGCTTTGGCAGAGATCGAAGGAGCTCTGGAGGTGCTAGTGAAGCTGATCAAAGAACCAGTTTGCCCTACTTCTACAAAAGCGTCCTTGGTCATCATGTATCAAATGGTCAACTCATCATCCTCCTCTCTTTCGAAAGAGAAAATCAAAGAGAGATTTGTAGAGCTGGGATTGGTGTCGGTGCTTTCAGACATTCTTGTAGATGCCGAAAGAAGCATTTGCGAGAAGGCGTTGGGAGTGCTGGACGGACTTTGCGGAAGCAAGCAAGGCAGGGAAAAGGCCTACCTTCATGCCTTGACCATGCCGGTTGTGGTGAAGAAGATACTTAGGGTTTCGGATTTGGCCACGGAGTTTTCCGTGTCTATTCTTTGGAAGCTTTGCAAGAACGAGACTAGGGAAGACGGAGGTGTGCTCGTGGAGGCCCTTCAAGTGGGTGCGTTTCAGAAGCTCTTGCTACTTTTGCAAGTTGGTTGTGCGGAGAGGACCAAAGACAAGGTCACGGACTTGTTAAAGGCGTTGAACATTCATAGGGAAAGGTTGGAGTGTATTGATTCTATGGATTTTAAGCAGCTCAAGAGGCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

444

Amino Acids

49.12

Weight (kDa)

7.11

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 27 - 98 7.3e-22 U-box domain
ARM_PUB PF25598 117 - 438 6e-69 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 415
AccII CGCG 1 cut(s) 36
AciI CCGC 8 cut(s) 34, 36, 259, 500, 590, 711, 999, 1220
AclI AACGTT 1 cut(s) 543
AclWI GGATC 5 cut(s) 119, 132, 155, 230, 628
AcoI YGGCCR 2 cut(s) 31, 1086
AcvI CACGTG 1 cut(s) 201
AfaI GTAC 2 cut(s) 155, 614
AfiI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
AgsI TTSAA 4 cut(s) 185, 522, 1172, 1264
AhdI GACNNNNNGTC 1 cut(s) 381
AjiI CACGTC 1 cut(s) 177
AluBI AGCT 8 cut(s) 73, 231, 770, 790, 910, 1120, 1192, 1315
AluI AGCT 8 cut(s) 73, 231, 770, 790, 910, 1120, 1192, 1315
Alw21I GWGCWC 3 cut(s) 702, 772, 1158
Alw26I GTCTC 4 cut(s) 167, 701, 721, 1127
AlwI GGATC 5 cut(s) 119, 132, 155, 230, 628
AoxI GGCC 6 cut(s) 31, 57, 1023, 1086, 1164, 1322
ApeKI GCWGC 2 cut(s) 41, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 1224, 1256
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 1165, 1227
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 361, 1069
AsuII TTCGAA 1 cut(s) 878
AvaII GGWCC 1 cut(s) 1227
BaeGI GKGCMC 2 cut(s) 101, 206
BalI TGGCCA 1 cut(s) 1088
BamHI GGATCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 772
BauI CACGAG 1 cut(s) 1157
BbrPI CACGTG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 1149
Bbv12I GWGCWC 3 cut(s) 702, 772, 1158
BbvI GCAGC 2 cut(s) 28, 1324
BccI CCATC 2 cut(s) 263, 543
BceAI ACGGC 1 cut(s) 533
BclI TGATCA 3 cut(s) 724, 730, 792
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 4 cut(s) 167, 701, 721, 1127
BfaI CTAG 4 cut(s) 477, 497, 782, 1137
BisI GCNGC 3 cut(s) 34, 42, 1313
BlsI GCNGC 3 cut(s) 35, 43, 1314
Bme1390I CCNGG 1 cut(s) 145
Bme18I GGWCC 1 cut(s) 1227
BmeRI GACNNNNNGTC 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 2 cut(s) 1165, 1227
BmiI GGNNCC 2 cut(s) 98, 126
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 3 cut(s) 698, 736, 937
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 1149
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 1 cut(s) 794
Bpu14I TTCGAA 1 cut(s) 878
BpuEI CTTGAG 2 cut(s) 131, 1301
BpuMI CCSGG 1 cut(s) 145
Bsa29I ATCGAT 1 cut(s) 744
BsaAI YACGTR 2 cut(s) 153, 201
BsaBI GATNNNNATC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 5 cut(s) 34, 45, 616, 831, 1089
BsaWI WCCGGW 2 cut(s) 93, 127
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 415, 1174
Bse118I RCCGGY 4 cut(s) 29, 59, 188, 1048
Bse1I ACTGG 1 cut(s) 803
Bse8I GATNNNNATC 1 cut(s) 729
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 5 cut(s) 34, 45, 616, 831, 1089
BseGI GGATG 3 cut(s) 255, 406, 863
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 803
BseRI GAGGAG 2 cut(s) 572, 859
BseSI GKGCMC 2 cut(s) 101, 206
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 28, 1324
BseYI CCCAGC 1 cut(s) 910
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 2 cut(s) 34, 160
BshFI GGCC 6 cut(s) 33, 59, 1025, 1088, 1166, 1324
BshNI GGYRCC 1 cut(s) 96
BshVI ATCGAT 1 cut(s) 744
BsiEI CGRYCG 2 cut(s) 34, 160
BsiHKAI GWGCWC 3 cut(s) 702, 772, 1158
BsiSI CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
BsmAI GTCTC 4 cut(s) 167, 701, 721, 1127
BsmBI CGTCTC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 312, 327
BsnI GGCC 6 cut(s) 33, 59, 1025, 1088, 1166, 1324
Bso31I GGTCTC 1 cut(s) 701
Bsp119I TTCGAA 1 cut(s) 878
Bsp1286I GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
BspACI CCGC 8 cut(s) 34, 36, 259, 500, 590, 711, 999, 1220
BspANI GGCC 6 cut(s) 33, 59, 1025, 1088, 1166, 1324
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 744
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 2 cut(s) 388, 727
BspLI GGNNCC 2 cut(s) 98, 126
BspPI GGATC 5 cut(s) 119, 132, 155, 230, 628
BspT104I TTCGAA 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 701
BsrFI RCCGGY 4 cut(s) 29, 59, 188, 1048
BsrI ACTGG 1 cut(s) 803
BssAI RCCGGY 4 cut(s) 29, 59, 188, 1048
BssECI CCNNGG 5 cut(s) 34, 45, 616, 831, 1089
BssSI CACGAG 1 cut(s) 1157
BssT1I CCWWGG 2 cut(s) 45, 831
Bst2BI CACGAG 1 cut(s) 1157
Bst4CI ACNGT 1 cut(s) 161
BstBAI YACGTR 2 cut(s) 153, 201
BstBI TTCGAA 1 cut(s) 878
BstC8I GCNNGC 5 cut(s) 31, 61, 190, 452, 1007
BstDEI CTNAG 2 cut(s) 23, 1070
BstDSI CCRYGG 2 cut(s) 34, 1089
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 3 cut(s) 255, 406, 863
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 312
BstMAI GTCTC 4 cut(s) 167, 701, 721, 1127
BstMCI CGRYCG 2 cut(s) 34, 160
BstMWI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 787, 1011, 1204, 1321
BstPAI GACNNNNGTC 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 143
BstSLI GKGCMC 2 cut(s) 101, 206
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 2 cut(s) 28, 1324
BstV2I GAAGAC 1 cut(s) 1149
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 744
BsuRI GGCC 6 cut(s) 33, 59, 1025, 1088, 1166, 1324
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 2 cut(s) 34, 1089
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 255, 406, 863
Cac8I GCNNGC 5 cut(s) 31, 61, 190, 452, 1007
CciI TCATGA 2 cut(s) 388, 727
CfoI GCGC 1 cut(s) 312
Cfr10I RCCGGY 4 cut(s) 29, 59, 188, 1048
Cfr13I GGNCC 2 cut(s) 1165, 1227
Cfr42I CCGCGG 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 744
CseI GACGC 2 cut(s) 271, 816
Csp6I GTAC 2 cut(s) 154, 613
CviAII CATG 8 cut(s) 12, 67, 389, 643, 728, 841, 1034, 1045
CviQI GTAC 2 cut(s) 154, 613
DdeI CTNAG 2 cut(s) 23, 1070
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 2 cut(s) 31, 1086
EagI CGGCCG 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 770
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 45, 831
Eco147I AGGCCT 2 cut(s) 1025, 1324
Eco24I GRGCYC 1 cut(s) 772
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 1227
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 770
Eco72I CACGTG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 770
EcoO109I RGGNCCY 1 cut(s) 1165
EcoO65I GGTNACC 1 cut(s) 349
EcoT14I CCWWGG 2 cut(s) 45, 831
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 45, 831
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 8 cut(s) 15, 70, 392, 646, 731, 844, 1037, 1048
FatI CATG 8 cut(s) 11, 66, 388, 642, 727, 840, 1033, 1044
FauI CCCGC 2 cut(s) 493, 597
FbaI TGATCA 3 cut(s) 724, 730, 792
Fnu4HI GCNGC 3 cut(s) 34, 42, 1313
FokI GGATG 3 cut(s) 242, 413, 850
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 3 cut(s) 34, 42, 1313
FspBI CTAG 4 cut(s) 477, 497, 782, 1137
GlaI GCGC 1 cut(s) 311
GluI GCNGC 3 cut(s) 34, 42, 1313
GsaI CCCAGC 1 cut(s) 914
GsuI CTGGAG 1 cut(s) 794
HaeIII GGCC 6 cut(s) 33, 59, 1025, 1088, 1166, 1324
HapII CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
HgaI GACGC 2 cut(s) 271, 816
HhaI GCGC 1 cut(s) 312
Hin1II CATG 8 cut(s) 15, 70, 392, 646, 731, 844, 1037, 1048
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 2 cut(s) 229, 1118
HinfI GANTC 3 cut(s) 303, 382, 1295
HpaII CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
HphI GGTGA 2 cut(s) 361, 1069
Hpy166II GTNNAC 2 cut(s) 141, 856
Hpy188I TCNGA 8 cut(s) 165, 333, 620, 724, 738, 934, 1081, 1188
Hpy188III TCNNGA 5 cut(s) 286, 389, 728, 773, 1318
Hpy8I GTNNAC 2 cut(s) 141, 856
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 7 cut(s) 12, 115, 636, 758, 964, 1040, 1178
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 4 cut(s) 152, 176, 200, 543
HpyCH4V TGCA 5 cut(s) 468, 515, 642, 1125, 1207
HpyF10VI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 787, 1011, 1204, 1321
HpyF3I CTNAG 2 cut(s) 23, 1070
HpySE526I ACGT 4 cut(s) 152, 176, 200, 543
Hsp92II CATG 8 cut(s) 15, 70, 392, 646, 731, 844, 1037, 1048
HspAI GCGC 1 cut(s) 310
KroI GCCGGC 3 cut(s) 29, 59, 188
KroNI GCCGGC 3 cut(s) 31, 61, 190
Ksp22I TGATCA 3 cut(s) 724, 730, 792
KspI CCGCGG 1 cut(s) 37
LmnI GCTCC 3 cut(s) 70, 489, 767
Lsp1109I GCAGC 2 cut(s) 28, 1324
LweI GCATC 3 cut(s) 698, 736, 937
MaeI CTAG 4 cut(s) 477, 497, 782, 1137
MaeII ACGT 4 cut(s) 152, 176, 200, 543
MaeIII GTNAC 4 cut(s) 130, 349, 455, 1240
MboII GAAGA 6 cut(s) 436, 621, 751, 1072, 1075, 1154
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
MlsI TGGCCA 1 cut(s) 1088
MluCI AATT 2 cut(s) 102, 240
MluNI TGGCCA 1 cut(s) 1088
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 1263
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 1 cut(s) 517
MroNI GCCGGC 3 cut(s) 29, 59, 188
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 3 cut(s) 510, 1253, 1308
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 2 cut(s) 36, 713
MspI CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
MspR9I CCNGG 1 cut(s) 145
Mva1269I GAATGC 2 cut(s) 312, 327
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 7 cut(s) 460, 489, 512, 787, 1011, 1204, 1321
NaeI GCCGGC 3 cut(s) 31, 61, 190
NciI CCSGG 1 cut(s) 145
NgoMIV GCCGGC 3 cut(s) 29, 59, 188
NlaIII CATG 8 cut(s) 15, 70, 392, 646, 731, 844, 1037, 1048
NlaIV GGNNCC 2 cut(s) 98, 126
NmuCI GTSAC 3 cut(s) 130, 349, 1240
NsiI ATGCAT 1 cut(s) 517
NspV TTCGAA 1 cut(s) 878
PagI TCATGA 2 cut(s) 388, 727
PceI AGGCCT 2 cut(s) 1025, 1324
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PctI GAATGC 2 cut(s) 312, 327
PdiI GCCGGC 3 cut(s) 31, 61, 190
PfeI GAWTC 2 cut(s) 303, 1295
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 2 cut(s) 7, 1238
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 3 cut(s) 35, 43, 1314
PleI GAGTC 1 cut(s) 390
PmaCI CACGTG 1 cut(s) 201
PmlI CACGTG 1 cut(s) 201
PpsI GAGTC 1 cut(s) 390
Ppu21I YACGTR 2 cut(s) 153, 201
PshAI GACNNNNGTC 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 772
Psp1406I AACGTT 1 cut(s) 543
PspCI CACGTG 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 910
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 98, 126
PspPI GGNCC 2 cut(s) 1165, 1227
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 2 cut(s) 7, 1238
RsaI GTAC 2 cut(s) 155, 614
RsaNI GTAC 2 cut(s) 154, 613
SacI GAGCTC 1 cut(s) 772
SacII CCGCGG 1 cut(s) 37
SaqAI TTAA 3 cut(s) 510, 1253, 1308
SatI GCNGC 3 cut(s) 34, 42, 1313
Sau96I GGNCC 2 cut(s) 1165, 1227
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 145
SduI GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
SfaNI GCATC 3 cut(s) 698, 736, 937
Sfr303I CCGCGG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 878
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 1 cut(s) 1227
SmlI CTYRAG 2 cut(s) 110, 1316
SmoI CTYRAG 2 cut(s) 110, 1316
Sse9I AATT 2 cut(s) 102, 240
SseBI AGGCCT 2 cut(s) 1025, 1324
SsiI CCGC 8 cut(s) 34, 36, 259, 500, 590, 711, 999, 1220
SspMI CTAG 4 cut(s) 477, 497, 782, 1137
SstI GAGCTC 1 cut(s) 772
StuI AGGCCT 2 cut(s) 1025, 1324
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 2 cut(s) 45, 831
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 4 cut(s) 155, 179, 203, 546
TaqI TCGA 7 cut(s) 234, 285, 306, 380, 744, 762, 878
TaqII GACCGA 1 cut(s) 368
TasI AATT 2 cut(s) 102, 240
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 303, 1295
Tru1I TTAA 3 cut(s) 510, 1253, 1308
Tru9I TTAA 3 cut(s) 510, 1253, 1308
TseFI GTSAC 3 cut(s) 130, 349, 1240
TseI GCWGC 2 cut(s) 41, 1312
Tsp45I GTSAC 3 cut(s) 130, 349, 1240
TspDTI ATGAA 3 cut(s) 134, 1022, 1259
TspGWI ACGGA 5 cut(s) 1005, 1090, 1106, 1161, 1259
Tth111I GACNNNGTC 2 cut(s) 7, 1238
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 1227
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XspI CTAG 4 cut(s) 477, 497, 782, 1137
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.