Rh4CG420500

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001443
Physical Location & Seq
Forward (+)
66445472 .. 66447185
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG420500.1

Sequence Viewer

Length: 1341 bp
ATGACTTTGTCATGGAGAAGGCTGAGGCCCGGCCGTCACGCCGCGAAACATTCACGGCATGGCGAGGCCGGCGGCGACATTGAGCTGACGATACCGAGCCACTTCCGGTGCCCGATCTCGTTGGACTTGATGAAAGATCCCGTCACGTTGTCTACCGGGATCACATACGATCGGCAGAGCATTGAGAAATGGATCGAAGCCGGAAACGTGACTTGCCCTACTACCAACCAGGTACTCACAACTTTGGAGCCGATGCCGAATCACACCATCAGGAAAATGATACAAGACTGGTGCGTGGAAAAGAAGTCCTTCGGCGTGGAGCGTATCCCCACGCCACGGATTCCGGTGAGTTCTGTCGAAGTCGCCGAGATTCTTTCGAGGATTACGTCGTCGGCGGAAAAGAAGGACGAAGCCGGCTGCAGAGAGCTGGTGCAGAAGGTCAAGGCGTTGACTAAAGAAAGCGAGCGCAACAAGCGGTGCATTGTCGCTAACGGAGCGGCGAGTTCTTTAGCGGCGGCGTTTGGTGCTTTTTCAACGGCGGGTTCTTTTCATGAAAACGTTGGCGTTTTGGAGGAGATATTGTCTGCTCTGACGTTGATTTTCCCGCTTCATTCGGAGGCCAACGTATATCTAGGATCCACAGATTCGTTGCATTGCATGGTGAGGTTTTTGGACGGTGGAGATTTGTCACAGAGAAGGAACGCAGTCTTGGTTCTAAAAGAGGCTCTTTCAGCTGATAAGCAAAAGACTGATGCTTTGGAAGAGATTGAAGGAGCATTGGAAGCCCTAGTGAAGCTGATCAAAGAGCCAATTTGCCCTACTTCTACTAAAGCTTCGTTGGTCATCATTTACCACATGCTTAATTCCACGTCTTCTAGCTCAAGAGAGAGCGTCAAAGGAAAATTTGTGCAAATGGGATTGGTGTCCATGCTATCGGAATTAGTTGTGGATGCCGAGAGGAGCATTTGCGAAAAGGCGTTGGGGGTTCTCGATGCGATTTGTGGAAGCAAGCAAGGGAGGGAAGAGGCCCGCGACAATGCCCTGATCATGCCGGTCGTAGTCAAGAAGATGCTTAGGGTTTCGGATTTAGCTACCGAGTTTGCTGTGTCCATTCTTTGGAAGCTTTGCAAGAAGGACGAGAGGGAAGACGACGAGGGAGGTCTTGTTCTAGAGGTGCTCCAAGTGGGAGCTTTTCAGAAGCTGTTGTTGCTGTTGCAAGTTGGGTGTGGGGAGAGGACTAAAGAGAAGGCGACCGAGTTGTTGAAGTTGTTGAATCTTCACAGGGCGAGGTTGGAGTGCATTGAGTCCATGGATTTTAAGGAGCTCAAGAGGCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

446

Amino Acids

49.25

Weight (kDa)

7.12

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 31 - 102 2.1e-21 U-box domain
ARM_PUB PF25598 120 - 441 4.2e-73 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 1 cut(s) 152
AccII CGCG 2 cut(s) 44, 1032
AclI AACGTT 1 cut(s) 558
AclWI GGATC 5 cut(s) 131, 167, 200, 630, 643
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 902
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 2 cut(s) 336, 1116
AgsI TTSAA 4 cut(s) 534, 770, 1264, 1273
AjiI CACGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
Alw21I GWGCWC 2 cut(s) 1179, 1326
AlwI GGATC 5 cut(s) 131, 167, 200, 630, 643
AlwNI CAGNNNCTG 1 cut(s) 1201
AoxI GGCC 6 cut(s) 26, 31, 66, 618, 1026, 1331
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 739, 771
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 3 cut(s) 27, 1027, 1332
AsuC2I CCSGG 2 cut(s) 30, 157
AsuHPI GGTGA 2 cut(s) 358, 673
BaeGI GKGCMC 1 cut(s) 113
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 1326
BbsI GAAGAC 2 cut(s) 864, 1152
Bbv12I GWGCWC 2 cut(s) 1179, 1326
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 404
BccI CCATC 1 cut(s) 275
BceAI ACGGC 3 cut(s) 18, 71, 552
BciT130I CCWGG 1 cut(s) 230
BciVI GTATCC 1 cut(s) 335
BclI TGATCA 2 cut(s) 798, 1044
BcnI CCSGG 2 cut(s) 30, 157
BfaI CTAG 4 cut(s) 632, 788, 876, 1169
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 335
BisI GCNGC 6 cut(s) 42, 73, 418, 498, 513, 516
BlsI GCNGC 6 cut(s) 43, 74, 419, 499, 514, 517
Bme1390I CCNGG 3 cut(s) 30, 157, 230
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 3 cut(s) 27, 1027, 1332
BmiI GGNNCC 3 cut(s) 110, 249, 637
BmrFI CCNGG 3 cut(s) 30, 157, 230
BmsI GCATC 5 cut(s) 243, 742, 940, 982, 1059
BpiI GAAGAC 2 cut(s) 864, 1152
Bpu10I CCTNAGC 2 cut(s) 23, 1073
BpuEI CTTGAG 2 cut(s) 865, 1310
BpuMI CCSGG 2 cut(s) 30, 157
BsaJI CCNNGG 2 cut(s) 335, 1308
BsaWI WCCGGW 2 cut(s) 105, 343
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 1149, 1179
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 1116
Bse118I RCCGGY 3 cut(s) 68, 413, 1051
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 652
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 335, 1308
BseGI GGATG 1 cut(s) 955
BseLI CCNNNNNNNGG 2 cut(s) 336, 1116
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 587, 973
BseSI GKGCMC 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 2 cut(s) 44, 1032
Bsh1285I CGRYCG 4 cut(s) 34, 172, 1056, 1254
BshFI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BshNI GGYRCC 1 cut(s) 108
BsiEI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BsiHKAI GWGCWC 2 cut(s) 1179, 1326
BsiSI CCGG 8 cut(s) 30, 69, 106, 156, 201, 344, 414, 1052
BslI CCNNNNNNNGG 2 cut(s) 336, 1116
BsnI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
Bsp1286I GDGCHC 3 cut(s) 113, 1179, 1326
Bsp143I GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
Bsp19I CCATGG 1 cut(s) 1308
BspANI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BspCNI CTCAG 1 cut(s) 15
BspFNI CGCG 2 cut(s) 44, 1032
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 3 cut(s) 110, 249, 637
BspMAI CTGCAG 1 cut(s) 422
BspPI GGATC 5 cut(s) 131, 167, 200, 630, 643
BspT107I GGYRCC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 652
BsrFI RCCGGY 3 cut(s) 68, 413, 1051
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 3 cut(s) 68, 413, 1051
BssECI CCNNGG 2 cut(s) 335, 1308
BssMI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
BssT1I CCWWGG 1 cut(s) 1308
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 677
Bst6I CTCTTC 2 cut(s) 756, 1017
BstC8I GCNNGC 5 cut(s) 70, 415, 464, 1010, 1030
BstDEI CTNAG 2 cut(s) 23, 1073
BstDSI CCRYGG 2 cut(s) 335, 1308
BstF5I GGATG 1 cut(s) 955
BstFNI CGCG 2 cut(s) 44, 1032
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 8 cut(s) 117, 139, 162, 172, 195, 638, 801, 1047
BstMBI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
BstMCI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BstMWI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 3 cut(s) 28, 155, 228
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 2 cut(s) 44, 1032
BstV1I GCAGC 1 cut(s) 404
BstV2I GAAGAC 2 cut(s) 864, 1152
BstX2I RGATCY 2 cut(s) 136, 635
BstYI RGATCY 2 cut(s) 136, 635
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BtgI CCRYGG 2 cut(s) 335, 1308
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 1 cut(s) 955
Cac8I GCNNGC 5 cut(s) 70, 415, 464, 1010, 1030
CaiI CAGNNNCTG 1 cut(s) 1201
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 468
Cfr10I RCCGGY 3 cut(s) 68, 413, 1051
Cfr13I GGNCC 3 cut(s) 27, 1027, 1332
CseI GACGC 1 cut(s) 880
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 233
CviAII CATG 8 cut(s) 12, 59, 551, 658, 856, 928, 1048, 1309
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 2 cut(s) 23, 1073
DpnI GATC 8 cut(s) 116, 138, 161, 171, 194, 637, 800, 1046
DpnII GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 756, 1017
EarI CTCTTC 2 cut(s) 756, 1017
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 1324
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 1308
Eco24I GRGCYC 1 cut(s) 1326
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 1324
EcoICRI GAGCTC 1 cut(s) 1324
EcoO109I RGGNCCY 1 cut(s) 1332
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 1308
EcoT38I GRGCYC 1 cut(s) 1326
ErhI CCWWGG 1 cut(s) 1308
FaeI CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
FalI AAGNNNNNCTT 5 cut(s) 293, 325, 711, 743, 1319
FatI CATG 8 cut(s) 11, 58, 550, 657, 855, 927, 1047, 1308
FauI CCCGC 3 cut(s) 532, 612, 1037
FbaI TGATCA 2 cut(s) 798, 1044
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 6 cut(s) 42, 73, 418, 498, 513, 516
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 1 cut(s) 1326
Fsp4HI GCNGC 6 cut(s) 42, 73, 418, 498, 513, 516
FspBI CTAG 4 cut(s) 632, 788, 876, 1169
GlaI GCGC 1 cut(s) 467
GluI GCNGC 6 cut(s) 42, 73, 418, 498, 513, 516
HaeIII GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
HapII CCGG 8 cut(s) 30, 69, 106, 156, 201, 344, 414, 1052
HgaI GACGC 1 cut(s) 880
HhaI GCGC 1 cut(s) 468
Hin1II CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 2 cut(s) 831, 1121
HinfI GANTC 6 cut(s) 259, 340, 370, 644, 1273, 1304
HpaII CCGG 8 cut(s) 30, 69, 106, 156, 201, 344, 414, 1052
HphI GGTGA 2 cut(s) 358, 673
Hpy166II GTNNAC 2 cut(s) 153, 450
Hpy188I TCNGA 5 cut(s) 591, 616, 937, 1084, 1197
Hpy188III TCNNGA 7 cut(s) 271, 551, 882, 989, 1063, 1169, 1327
Hpy8I GTNNAC 2 cut(s) 153, 450
Hpy99I CGWCG 3 cut(s) 391, 394, 1154
HpyAV CCTTC 8 cut(s) 12, 319, 397, 430, 690, 764, 1126, 1240
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 7 cut(s) 146, 207, 386, 558, 593, 624, 869
HpyCH4V TGCA 9 cut(s) 420, 433, 480, 652, 657, 910, 1128, 1216, 1299
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
HpyF3I CTNAG 2 cut(s) 23, 1073
HpySE526I ACGT 7 cut(s) 146, 207, 386, 558, 593, 624, 869
Hsp92II CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
HspAI GCGC 1 cut(s) 466
KroI GCCGGC 2 cut(s) 68, 413
KroNI GCCGGC 2 cut(s) 70, 415
Ksp22I TGATCA 2 cut(s) 798, 1044
Kzo9I GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
LmnI GCTCC 8 cut(s) 247, 319, 494, 773, 960, 1182, 1187, 1321
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 5 cut(s) 243, 742, 940, 982, 1059
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 4 cut(s) 632, 788, 876, 1169
MaeII ACGT 7 cut(s) 146, 207, 386, 558, 593, 624, 869
MaeIII GTNAC 4 cut(s) 35, 142, 208, 687
MalI GATC 8 cut(s) 116, 138, 161, 171, 194, 637, 800, 1046
MbiI CCGCTC 1 cut(s) 497
MboI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
MboII GAAGA 6 cut(s) 773, 864, 1034, 1078, 1157, 1268
MflI RGATCY 2 cut(s) 136, 635
MhlI GDGCHC 3 cut(s) 113, 1179, 1326
MluCI AATT 4 cut(s) 810, 862, 902, 938
MlyI GAGTC 1 cut(s) 1313
MmeI TCCRAC 2 cut(s) 102, 1272
MroNI GCCGGC 2 cut(s) 68, 413
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 2 cut(s) 861, 1317
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 8 cut(s) 30, 69, 106, 156, 201, 344, 414, 1052
MspR9I CCNGG 3 cut(s) 30, 157, 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 2 cut(s) 44, 1032
MwoI GCNNNNNNNGC 8 cut(s) 69, 472, 494, 524, 731, 782, 1207, 1330
NaeI GCCGGC 2 cut(s) 70, 415
NciI CCSGG 2 cut(s) 30, 157
NcoI CCATGG 1 cut(s) 1308
NdeII GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
NgoMIV GCCGGC 2 cut(s) 68, 413
NlaIII CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
NlaIV GGNNCC 3 cut(s) 110, 249, 637
NmeAIII GCCGAG 2 cut(s) 391, 979
NmuCI GTSAC 4 cut(s) 35, 142, 208, 687
NspI RCATGY 1 cut(s) 859
PagI TCATGA 1 cut(s) 550
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 383
PdiI GCCGGC 2 cut(s) 70, 415
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 5 cut(s) 259, 340, 370, 644, 1273
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 1116
PkrI GCNGC 6 cut(s) 43, 74, 419, 499, 514, 517
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 1 cut(s) 1312
PpsI GAGTC 1 cut(s) 1312
Psp124BI GAGCTC 1 cut(s) 1326
Psp1406I AACGTT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 3 cut(s) 110, 249, 637
PspPI GGNCC 3 cut(s) 27, 1027, 1332
PstI CTGCAG 1 cut(s) 422
PstNI CAGNNNCTG 1 cut(s) 1201
PsuI RGATCY 2 cut(s) 136, 635
PsyI GACNNNGTC 1 cut(s) 7
PvuI CGATCG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SacI GAGCTC 1 cut(s) 1326
SaqAI TTAA 2 cut(s) 861, 1317
SatI GCNGC 6 cut(s) 42, 73, 418, 498, 513, 516
Sau3AI GATC 8 cut(s) 114, 136, 159, 169, 192, 635, 798, 1044
Sau96I GGNCC 3 cut(s) 27, 1027, 1332
SchI GAGTC 1 cut(s) 1313
ScrFI CCNGG 3 cut(s) 30, 157, 230
SduI GDGCHC 3 cut(s) 113, 1179, 1326
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 5 cut(s) 243, 742, 940, 982, 1059
SfcI CTRYAG 1 cut(s) 418
SmlI CTYRAG 2 cut(s) 880, 1325
SmoI CTYRAG 2 cut(s) 880, 1325
Sse9I AATT 4 cut(s) 810, 862, 902, 938
SspMI CTAG 4 cut(s) 632, 788, 876, 1169
SstI GAGCTC 1 cut(s) 1326
StyD4I CCNGG 3 cut(s) 28, 155, 228
StyI CCWWGG 1 cut(s) 1308
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 7 cut(s) 149, 210, 389, 561, 596, 627, 872
TaqI TCGA 4 cut(s) 195, 357, 377, 990
TaqII GACCGA 1 cut(s) 1268
TasI AATT 4 cut(s) 810, 862, 902, 938
TauI GCSGC 5 cut(s) 44, 75, 500, 515, 518
TfiI GAWTC 5 cut(s) 259, 340, 370, 644, 1273
Tru1I TTAA 2 cut(s) 861, 1317
Tru9I TTAA 2 cut(s) 861, 1317
TseFI GTSAC 4 cut(s) 35, 142, 208, 687
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 4 cut(s) 35, 142, 208, 687
TspDTI ATGAA 4 cut(s) 146, 539, 567, 599
TspGWI ACGGA 2 cut(s) 352, 507
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 1116
XapI RAATTY 1 cut(s) 902
XbaI TCTAGA 1 cut(s) 1168
XceI RCATGY 1 cut(s) 859
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 4 cut(s) 632, 788, 876, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.