FvH4_5g17730

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001443
Physical Location & Seq
Reverse (-)
10156929 .. 10158284
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17730.t1

Sequence Viewer

Length: 1356 bp
ATGGTTTTGGGTTGGAGAAGAAGGCGCAGCACCACCAGCCACCGTGCCACCGCAGCGAAGAAGCAGCCTTCGCCTGGCAACCTCGACATGGACCAACTCGTGATCCCAAAGCATTTTCTCTGTCCGATATCTCTTGACTTGATGAAAGATCCGGTCACATTGTCCTCCGGGATAACCTACGATCGGCAAAGCATTGACACGTGGTTGGAAGCCGGGAACTTCAAGTGTCCGGTGACGAATCAGGTGCTGAGAAACTTTGATATGATTCCTAACCATACTCTCCGAACAATGATCCAAGAATGGAGCTCTCAGAACCAACGCTTCGGCATTCACCGTGTTCCAACTCCTAGAGTTCCCATCATGGCAGTTGAGGTGTCCGAGATTCTGTATAGCATCACAGCCTCAGCGAGACGCGTGGATCAAAATGGCTGCTTAGAGAGCGTGCAGAGGATCAAGAAATGGGCGGGTGAGGGTGACCGGAACAAGAGGTGCATCGTGGAGAATGGAGCGGCCTCTGTTCTTGCATCTGCATTTGATGCTTTTGCTAGCGATGGCATAAAGAAAAATGGTCGGCTGTGTGAAGAAATCTTATCAGCTTTGAGTTGGATGTTGTTTCAGTCATTAGATGAAGAAACCCAAAAGTACTTGGGATCTCATGCTTCTTTGGATTGCTTGGTTTGGTTCCTCAAGGGTGATAAAGATCCTTTGGTGAAGCAAAACTCGATTCTTGTATTGAAAGAGCTTCTGTCTAGTTGCAATGATCAGAACAAACGTGCAGTTGAAGCATTGGCAGAGATTGAAGGAGTCAGTAAGATACTTTTCGATTTCATCAAGGGGAAAGTTTCCATGACAATTACCAAGGCTTCTTTAACAGTAGTCTTCTACTTGGTTTCATTCAGTGAGAAGTTCAGATCAGCATTCTTGGAGATGGGTGTGGTTTCCCTTTTGTTAGAGACCATTCTCGACTCGGAGAATGGCATAACCGAGAGGGCTTTGTATATTTTGGATTGTCTTTGTGACTGCAAAGAAGGGAGAGAGCAGGCCTATGCTAATGCTTTGACTATACCAGTTTTGGTGAAGAAGATTTTGAGGGTGTCGGAAATGGCAACCGAGCATTCAATCTCTGCTGTTTGGAAGCTGTGCAAGAATGCGACTAGGCAAGAAGAGAGAGTACTTGTTGAGGCTCTTCAAGTTGGTGCATTTCAGAAGCTGTTGCTGGTTTTACAAATTCGTTGCGCAGGCGAATATGGCACAAAGGATAAAGCAACTGAGCTTTTGAAGCTCTTGAATCCATACAGAGCTGGATTGGAATGCATCGAGTCTGTAGATTTCAAGAGTATTAGAAGGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

452

Amino Acids

50.68

Weight (kDa)

8.58

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 35 - 106 8.2e-22 U-box domain
ARM_PUB PF25598 124 - 446 4.1e-65 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1237
AccBSI CCGCTC 1 cut(s) 509
AccII CGCG 1 cut(s) 414
AciI CCGC 3 cut(s) 51, 464, 509
AclWI GGATC 7 cut(s) 97, 143, 286, 426, 458, 658, 695
AcsI RAATTY 1 cut(s) 1227
AcvI CACGTG 1 cut(s) 201
AfaI GTAC 2 cut(s) 644, 1173
AfiI CCNNNNNNNGG 3 cut(s) 74, 88, 183
AflIII ACRYGT 2 cut(s) 198, 412
AgsI TTSAA 9 cut(s) 223, 736, 782, 800, 1119, 1190, 1279, 1288, 1333
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 8 cut(s) 306, 596, 742, 1138, 1210, 1273, 1282, 1301
AluI AGCT 8 cut(s) 306, 596, 742, 1138, 1210, 1273, 1282, 1301
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 403, 947
AlwI GGATC 7 cut(s) 97, 143, 286, 426, 458, 658, 695
AlwNI CAGNNNCTG 2 cut(s) 247, 1210
AoxI GGCC 2 cut(s) 510, 1041
ApeKI GCWGC 4 cut(s) 27, 53, 64, 429
ApoI RAATTY 1 cut(s) 1227
AspLEI GCGC 2 cut(s) 27, 1238
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 2 cut(s) 169, 214
AsuHPI GGTGA 7 cut(s) 244, 323, 479, 485, 704, 721, 1087
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 1155, 1187
BauI CACGAG 1 cut(s) 98
BbrPI CACGTG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 871
Bbv12I GWGCWC 1 cut(s) 308
BbvCI CCTCAGC 1 cut(s) 403
BbvI GCAGC 4 cut(s) 39, 65, 76, 416
BccI CCATC 3 cut(s) 365, 545, 922
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 75
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 2 cut(s) 169, 214
BcoDI GTCTC 2 cut(s) 403, 947
BfaI CTAG 4 cut(s) 348, 546, 750, 1155
BfmI CTRYAG 1 cut(s) 1323
BisI GCNGC 5 cut(s) 28, 54, 65, 430, 510
BlsI GCNGC 5 cut(s) 29, 55, 66, 431, 511
BmcAI AGTACT 2 cut(s) 644, 1173
Bme1390I CCNGG 3 cut(s) 75, 169, 214
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 683
BmrFI CCNGG 3 cut(s) 75, 169, 214
BmsI GCATC 5 cut(s) 402, 501, 526, 533, 1323
BmtI GCTAGC 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 871
Bpu10I CCTNAGC 1 cut(s) 403
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 2 cut(s) 169, 214
BsaAI YACGTR 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 699
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 1 cut(s) 858
BsaWI WCCGGW 3 cut(s) 151, 229, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 88, 183
Bse1I ACTGG 1 cut(s) 1067
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 858
BseGI GGATG 1 cut(s) 612
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 3 cut(s) 74, 88, 183
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 4 cut(s) 239, 323, 417, 1260
BseNI ACTGG 1 cut(s) 1067
BseXI GCAGC 4 cut(s) 39, 65, 76, 416
BsgI GTGCAG 2 cut(s) 464, 795
Bsh1236I CGCG 1 cut(s) 414
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 512, 1043
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 5 cut(s) 152, 168, 213, 230, 478
BslI CCNNNNNNNGG 3 cut(s) 74, 88, 183
BsmAI GTCTC 2 cut(s) 403, 947
BsmBI CGTCTC 1 cut(s) 403
BsmI GAATGC 5 cut(s) 327, 917, 1114, 1153, 1316
BsnI GGCC 2 cut(s) 512, 1043
Bso31I GGTCTC 1 cut(s) 947
Bsp1286I GDGCHC 1 cut(s) 308
BspACI CCGC 3 cut(s) 51, 464, 509
BspANI GGCC 2 cut(s) 512, 1043
BspCNI CTCAG 4 cut(s) 240, 322, 416, 1261
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 683
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 7 cut(s) 97, 143, 286, 426, 458, 658, 695
BspQI GCTCTTC 1 cut(s) 1191
BspTNI GGTCTC 1 cut(s) 947
BsrBI CCGCTC 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 1 cut(s) 1067
BssECI CCNNGG 1 cut(s) 858
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 858
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 3 cut(s) 44, 335, 874
Bst6I CTCTTC 2 cut(s) 1158, 1191
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 443, 547, 1041, 1240
BstDEI CTNAG 5 cut(s) 248, 309, 403, 433, 1269
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 2 cut(s) 27, 1238
BstMAI GTCTC 2 cut(s) 403, 947
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 8 cut(s) 36, 53, 70, 438, 536, 782, 1248, 1279
BstNI CCWGG 1 cut(s) 75
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 73, 167, 212
BstSFI CTRYAG 1 cut(s) 1323
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 4 cut(s) 39, 65, 76, 416
BstV2I GAAGAC 1 cut(s) 871
BstX2I RGATCY 3 cut(s) 148, 650, 700
BstYI RGATCY 3 cut(s) 148, 650, 700
BsuRI GGCC 2 cut(s) 512, 1043
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 904
Cac8I GCNNGC 4 cut(s) 443, 547, 1041, 1240
CaiI CAGNNNCTG 2 cut(s) 247, 1210
CfoI GCGC 2 cut(s) 27, 1238
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 420
Csp6I GTAC 2 cut(s) 643, 1172
CviAII CATG 4 cut(s) 88, 361, 656, 847
CviQI GTAC 2 cut(s) 643, 1172
DdeI CTNAG 5 cut(s) 248, 309, 403, 433, 1269
Eam1104I CTCTTC 2 cut(s) 1158, 1191
EarI CTCTTC 2 cut(s) 1158, 1191
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 858
Eco147I AGGCCT 1 cut(s) 1043
Eco24I GRGCYC 1 cut(s) 308
Eco31I GGTCTC 1 cut(s) 947
Eco32I GATATC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 306
Eco72I CACGTG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 73
EcoRV GATATC 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 1316
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 1 cut(s) 403
FaeI CATG 4 cut(s) 91, 364, 659, 850
FatI CATG 4 cut(s) 87, 360, 655, 846
FauI CCCGC 1 cut(s) 457
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 5 cut(s) 28, 54, 65, 430, 510
FokI GGATG 1 cut(s) 619
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 5 cut(s) 28, 54, 65, 430, 510
FspBI CTAG 4 cut(s) 348, 546, 750, 1155
FspI TGCGCA 1 cut(s) 1237
GlaI GCGC 2 cut(s) 26, 1237
GluI GCNGC 5 cut(s) 28, 54, 65, 430, 510
HaeIII GGCC 2 cut(s) 512, 1043
HapII CCGG 5 cut(s) 152, 168, 213, 230, 478
HgaI GACGC 1 cut(s) 420
HhaI GCGC 2 cut(s) 27, 1238
Hin1II CATG 4 cut(s) 91, 364, 659, 850
Hin6I GCGC 2 cut(s) 25, 1236
HinP1I GCGC 2 cut(s) 25, 1236
HinfI GANTC 8 cut(s) 238, 265, 382, 724, 804, 965, 1288, 1319
HpaII CCGG 5 cut(s) 152, 168, 213, 230, 478
HphI GGTGA 7 cut(s) 244, 323, 479, 485, 704, 721, 1087
Hpy188III TCNNGA 6 cut(s) 100, 134, 454, 962, 1285, 1333
HpyAV CCTTC 5 cut(s) 15, 78, 794, 1022, 1338
HpyCH4III ACNGT 3 cut(s) 44, 335, 874
HpyCH4IV ACGT 2 cut(s) 200, 772
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 53, 70, 438, 536, 782, 1248, 1279
HpyF3I CTNAG 5 cut(s) 248, 309, 403, 433, 1269
HpySE526I ACGT 2 cut(s) 200, 772
Hsp92II CATG 4 cut(s) 91, 364, 659, 850
HspAI GCGC 2 cut(s) 25, 1236
Ksp22I TGATCA 1 cut(s) 760
LguI GCTCTTC 1 cut(s) 1191
LmnI GCTCC 2 cut(s) 303, 506
Lsp1109I GCAGC 4 cut(s) 39, 65, 76, 416
LweI GCATC 5 cut(s) 402, 501, 526, 533, 1323
MaeI CTAG 4 cut(s) 348, 546, 750, 1155
MaeII ACGT 2 cut(s) 200, 772
MaeIII GTNAC 4 cut(s) 154, 232, 473, 1016
MbiI CCGCTC 1 cut(s) 509
MboII GAAGA 9 cut(s) 30, 70, 593, 641, 871, 1090, 1093, 1175, 1178
MflI RGATCY 3 cut(s) 148, 650, 700
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 2 cut(s) 852, 1227
MluI ACGCGT 1 cut(s) 412
MlyI GAGTC 3 cut(s) 813, 959, 1328
MmeI TCCRAC 4 cut(s) 186, 365, 584, 1077
Mph1103I ATGCAT 1 cut(s) 1316
MseI TTAA 1 cut(s) 869
MspI CCGG 5 cut(s) 152, 168, 213, 230, 478
MspR9I CCNGG 3 cut(s) 75, 169, 214
Mva1269I GAATGC 5 cut(s) 327, 917, 1114, 1153, 1316
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 8 cut(s) 36, 53, 70, 438, 536, 782, 1248, 1279
NciI CCSGG 2 cut(s) 169, 214
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 4 cut(s) 91, 364, 659, 850
NlaIV GGNNCC 1 cut(s) 683
NmuCI GTSAC 4 cut(s) 154, 232, 473, 1016
NsbI TGCGCA 1 cut(s) 1237
NsiI ATGCAT 1 cut(s) 1316
PceI AGGCCT 1 cut(s) 1043
PciSI GCTCTTC 1 cut(s) 1191
PctI GAATGC 5 cut(s) 327, 917, 1114, 1153, 1316
PfeI GAWTC 5 cut(s) 238, 265, 382, 724, 1288
PfoI TCCNGGA 1 cut(s) 167
PkrI GCNGC 5 cut(s) 29, 55, 66, 431, 511
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 3 cut(s) 812, 959, 1327
PmaCI CACGTG 1 cut(s) 201
PmlI CACGTG 1 cut(s) 201
PpsI GAGTC 3 cut(s) 812, 959, 1327
Ppu21I YACGTR 1 cut(s) 201
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 73
PspCI CACGTG 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 683
PspPI GGNCC 1 cut(s) 91
PstNI CAGNNNCTG 2 cut(s) 247, 1210
PsuI RGATCY 3 cut(s) 148, 650, 700
PvuI CGATCG 1 cut(s) 184
RsaI GTAC 2 cut(s) 644, 1173
RsaNI GTAC 2 cut(s) 643, 1172
SacI GAGCTC 1 cut(s) 308
SapI GCTCTTC 1 cut(s) 1191
SaqAI TTAA 1 cut(s) 869
SatI GCNGC 5 cut(s) 28, 54, 65, 430, 510
Sau96I GGNCC 1 cut(s) 91
ScaI AGTACT 2 cut(s) 644, 1173
SchI GAGTC 3 cut(s) 813, 959, 1328
ScrFI CCNGG 3 cut(s) 75, 169, 214
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 5 cut(s) 402, 501, 526, 533, 1323
SfcI CTRYAG 1 cut(s) 1323
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 2 cut(s) 852, 1227
SseBI AGGCCT 1 cut(s) 1043
SsiI CCGC 3 cut(s) 51, 464, 509
SspMI CTAG 4 cut(s) 348, 546, 750, 1155
SstI GAGCTC 1 cut(s) 308
StuI AGGCCT 1 cut(s) 1043
StyD4I CCNGG 3 cut(s) 73, 167, 212
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 3 cut(s) 44, 335, 874
TaiI ACGT 2 cut(s) 203, 775
TaqI TCGA 5 cut(s) 84, 722, 822, 963, 1317
TasI AATT 2 cut(s) 852, 1227
TatI WGTACW 2 cut(s) 642, 1171
TauI GCSGC 1 cut(s) 512
TfiI GAWTC 5 cut(s) 238, 265, 382, 724, 1288
Tru1I TTAA 1 cut(s) 869
Tru9I TTAA 1 cut(s) 869
TscAI CASTG 1 cut(s) 904
TseFI GTSAC 4 cut(s) 154, 232, 473, 1016
TseI GCWGC 4 cut(s) 27, 53, 64, 429
Tsp45I GTSAC 4 cut(s) 154, 232, 473, 1016
TspDTI ATGAA 4 cut(s) 158, 642, 817, 882
TspRI CASTG 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 1227
XspI CTAG 4 cut(s) 348, 546, 750, 1155
ZrmI AGTACT 2 cut(s) 644, 1173
Zsp2I ATGCAT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.