Rroxscaffold_3G00275460

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001443
Physical Location & Seq
Reverse (-)
66916612 .. 66918274
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275460.1

Sequence Viewer

Length: 1269 bp
ATGGATCAACTCGTGATCCCAAAGCATTTCCTCTGCCCAATATCTCTCGACTTGATGAAGGATCCCGTCACATTGTCCTCAGGGATAACTTATGATCGGCAGAGCATCGACACTTGGCTCGAAGCCGGCAACTTCAAGTGCCCGGTGACCAATCAGGTGCTGAGGAACTTTGATATGATTCCCAACCACACTCTCCGAACAATGATTCAAGATTGGAGCGCTCAAAACCAACGCTACGGCATTCAGCGTGTCCCAACTCCTAGAATTCCTATCATGGCAGTTGAGGTCTCTGAGATTCTGTTTAGCATCACAGCCTCAGCGAGACGCGTCGATCAAAATGGTTGCTTAGAGTGCGTGCAGATAATCAAGAAATGGGCGGCTGAGGGCGACAGAAACAAGCGGTGCATCGTGGAGAATGGAGCGGCCTCTGTTCTTGCTTCTGCTTTTGATACTTTTGCAAGTGATGGAATAAAGAAAAATGCTGGAGTGTGTGAAGAAATTTTGTCAGCTCTGAGTTGGATGATGTTTCCATCATTAGATGAAGAAACCCAAAAGTACTTGGGATCTCATGCTTCTTTGGATTGCCTGGTTTGGTTCCTCAAGGTTGATGGAGATCTTTTGGTGAAGCAAAACTCAATTCTAGCATTGAAAGAGCTTCTTTCTAATAGCAATGATCAGAACAAACACGCGGTTGAAGCATTGGCAGAGATTGAAGGAGTCAATCAGATACTTTTCGGTTTCATCAAGGAGAAAAGTTCCATGACGATTACCAAGTCTTCTTTAACAGTAGTCTTCTACTTGGTGTCATTCAGTGAGAAGTTGAGATCAGCATTCTTGGAGATGGGTTTGGTTTCCCTATTGTTAGAGACCATTCTCGACTCGGAAAAAGGCATAACGGAGAGGGCTTTGAATGTTTTGGATTGTCTTTGTGATTGCAAAGAAGGGAGGGAACAGGCCTATGCTAATGCGCTGACTATACCAGTTTTGGTGAAGAAGATTTTGAGGGTGTCGGAAATGGCGACTGAGTATTCGATCTCTGCTGTTTGGAAGCTGTGTAAGAATGCAACTCGGCAAGAAGAGAGAGTACTTGTTGAGGCTCTTCAAGTTGGTGCATTTCAGAAGCTCTTGCTGGTTTTACAGGTTCGTTGCGCCGGTGAATATGACACAAAGGACAAACCAACTGAGCTTTTGAAGCTGTTGAATCCTTACAGAGCTGGATTGGAATGCATCGAGTCTGTAGATTTCAAGAGTATTAGAAGGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

422

Amino Acids

47.32

Weight (kDa)

5.66

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 6 - 77 1.3e-21 U-box domain
ARM_PUB PF25598 95 - 417 1.1e-63 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 422
AccII CGCG 2 cut(s) 327, 689
AciI CCGC 4 cut(s) 377, 400, 422, 689
AclWI GGATC 5 cut(s) 10, 12, 56, 69, 571
AcsI RAATTY 2 cut(s) 264, 498
AfaI GTAC 2 cut(s) 557, 1086
AfeI AGCGCT 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 325
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 7 cut(s) 509, 655, 1051, 1123, 1186, 1195, 1214
AluI AGCT 7 cut(s) 509, 655, 1051, 1123, 1186, 1195, 1214
Alw26I GTCTC 3 cut(s) 292, 316, 860
AlwI GGATC 5 cut(s) 10, 12, 56, 69, 571
AlwNI CAGNNNCTG 1 cut(s) 160
Aor51HI AGCGCT 1 cut(s) 220
AoxI GGCC 2 cut(s) 423, 954
ApoI RAATTY 2 cut(s) 264, 498
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
AspLEI GCGC 3 cut(s) 221, 970, 1151
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 4 cut(s) 157, 634, 1000, 1166
AxyI CCTNAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 1068, 1100
BauI CACGAG 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 768, 784
BbvCI CCTCAGC 3 cut(s) 161, 316, 381
BccI CCATC 4 cut(s) 458, 538, 602, 835
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 587
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 3 cut(s) 292, 316, 860
BfaI CTAG 2 cut(s) 261, 641
BfmI CTRYAG 1 cut(s) 1236
BfoI RGCGCY 1 cut(s) 222
BglII AGATCT 1 cut(s) 613
BisI GCNGC 2 cut(s) 378, 423
BlsI GCNGC 2 cut(s) 379, 424
BmcAI AGTACT 2 cut(s) 557, 1086
Bme1390I CCNGG 2 cut(s) 143, 587
BmiI GGNNCC 2 cut(s) 63, 596
BmrFI CCNGG 2 cut(s) 143, 587
BmsI GCATC 4 cut(s) 114, 315, 414, 1236
BpiI GAAGAC 2 cut(s) 768, 784
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 3 cut(s) 161, 316, 381
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 612
BsaI GGTCTC 2 cut(s) 292, 860
Bse118I RCCGGY 2 cut(s) 125, 1151
Bse1I ACTGG 1 cut(s) 980
Bse21I CCTNAGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 676
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 1 cut(s) 587
BseGI GGATG 1 cut(s) 525
BseJI GATNNNNATC 1 cut(s) 612
BseMI GCAATG 1 cut(s) 676
BseMII CTCAG 8 cut(s) 93, 152, 282, 330, 372, 503, 1014, 1173
BseNI ACTGG 1 cut(s) 980
BseSI GKGCMC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 2 cut(s) 327, 689
BshFI GGCC 2 cut(s) 425, 956
BsiSI CCGG 3 cut(s) 126, 143, 1152
BslFI GGGAC 1 cut(s) 236
BsmAI GTCTC 3 cut(s) 292, 316, 860
BsmBI CGTCTC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 236
BsmI GAATGC 4 cut(s) 240, 830, 1066, 1229
BsnI GGCC 2 cut(s) 425, 956
Bso31I GGTCTC 2 cut(s) 292, 860
Bsp1286I GDGCHC 1 cut(s) 143
BspACI CCGC 4 cut(s) 377, 400, 422, 689
BspANI GGCC 2 cut(s) 425, 956
BspCNI CTCAG 8 cut(s) 92, 153, 283, 329, 373, 504, 1015, 1174
BspFNI CGCG 2 cut(s) 327, 689
BspLI GGNNCC 2 cut(s) 63, 596
BspPI GGATC 5 cut(s) 10, 12, 56, 69, 571
BspQI GCTCTTC 1 cut(s) 1104
BspTNI GGTCTC 2 cut(s) 292, 860
BsrBI CCGCTC 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 676
BsrFI RCCGGY 2 cut(s) 125, 1151
BsrI ACTGG 1 cut(s) 980
BssAI RCCGGY 2 cut(s) 125, 1151
BssSI CACGAG 1 cut(s) 11
Bst2BI CACGAG 1 cut(s) 11
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 2 cut(s) 1071, 1104
BstC8I GCNNGC 2 cut(s) 127, 356
BstDEI CTNAG 9 cut(s) 79, 161, 291, 316, 346, 381, 512, 1023, 1182
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 2 cut(s) 327, 689
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 3 cut(s) 221, 970, 1151
BstMAI GTCTC 3 cut(s) 292, 316, 860
BstMWI GCNNNNNNNGC 3 cut(s) 351, 695, 1192
BstNI CCWGG 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 141, 585
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 2 cut(s) 327, 689
BstV2I GAAGAC 2 cut(s) 768, 784
BstX2I RGATCY 3 cut(s) 61, 563, 613
BstYI RGATCY 3 cut(s) 61, 563, 613
Bsu36I CCTNAGG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 425, 956
BtsCI GGATG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 817
Cac8I GCNNGC 2 cut(s) 127, 356
CaiI CAGNNNCTG 1 cut(s) 160
CfoI GCGC 3 cut(s) 221, 970, 1151
Cfr10I RCCGGY 2 cut(s) 125, 1151
CseI GACGC 2 cut(s) 316, 333
Csp6I GTAC 2 cut(s) 556, 1085
CviAII CATG 3 cut(s) 274, 569, 760
CviQI GTAC 2 cut(s) 556, 1085
DdeI CTNAG 9 cut(s) 79, 161, 291, 316, 346, 381, 512, 1023, 1182
Eam1104I CTCTTC 2 cut(s) 1071, 1104
EarI CTCTTC 2 cut(s) 1071, 1104
Eco147I AGGCCT 1 cut(s) 956
Eco31I GGTCTC 2 cut(s) 292, 860
Eco47III AGCGCT 1 cut(s) 220
Eco81I CCTNAGG 1 cut(s) 79
Eco91I GGTNACC 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 585
EcoT22I ATGCAT 1 cut(s) 1229
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 3 cut(s) 277, 572, 763
FaiI YATR 9 cut(s) 93, 176, 275, 570, 761, 893, 960, 977, 1161
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 273, 568, 759
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 378, 423
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 2 cut(s) 378, 423
FspBI CTAG 2 cut(s) 261, 641
GlaI GCGC 3 cut(s) 220, 969, 1150
GluI GCNGC 2 cut(s) 378, 423
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 2 cut(s) 425, 956
HapII CCGG 3 cut(s) 126, 143, 1152
HgaI GACGC 2 cut(s) 316, 333
HhaI GCGC 3 cut(s) 221, 970, 1151
Hin1II CATG 3 cut(s) 277, 572, 763
Hin6I GCGC 3 cut(s) 219, 968, 1149
HinP1I GCGC 3 cut(s) 219, 968, 1149
HinfI GANTC 7 cut(s) 178, 205, 295, 717, 878, 1201, 1232
HpaII CCGG 3 cut(s) 126, 143, 1152
HphI GGTGA 4 cut(s) 157, 634, 1000, 1166
Hpy188I TCNGA 9 cut(s) 197, 292, 513, 678, 726, 883, 1012, 1119, 1268
Hpy188III TCNNGA 6 cut(s) 13, 47, 209, 367, 875, 1246
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 4 cut(s) 52, 707, 935, 1251
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4V TGCA 7 cut(s) 358, 405, 458, 936, 1064, 1112, 1227
HpyF10VI GCNNNNNNNGC 3 cut(s) 351, 695, 1192
HpyF3I CTNAG 9 cut(s) 79, 161, 291, 316, 346, 381, 512, 1023, 1182
Hsp92II CATG 3 cut(s) 277, 572, 763
HspAI GCGC 3 cut(s) 219, 968, 1149
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 673
LguI GCTCTTC 1 cut(s) 1104
LmnI GCTCC 2 cut(s) 216, 419
LweI GCATC 4 cut(s) 114, 315, 414, 1236
MaeI CTAG 2 cut(s) 261, 641
MaeIII GTNAC 2 cut(s) 67, 145
MbiI CCGCTC 1 cut(s) 422
MboII GAAGA 8 cut(s) 506, 554, 768, 784, 1003, 1006, 1088, 1091
MflI RGATCY 3 cut(s) 61, 563, 613
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 3 cut(s) 264, 498, 636
MluI ACGCGT 1 cut(s) 325
MlyI GAGTC 3 cut(s) 726, 872, 1241
MmeI TCCRAC 2 cut(s) 497, 990
Mph1103I ATGCAT 1 cut(s) 1229
MroNI GCCGGC 1 cut(s) 125
MseI TTAA 1 cut(s) 782
MspI CCGG 3 cut(s) 126, 143, 1152
MspR9I CCNGG 2 cut(s) 143, 587
Mva1269I GAATGC 4 cut(s) 240, 830, 1066, 1229
MvaI CCWGG 1 cut(s) 587
MvnI CGCG 2 cut(s) 327, 689
MwoI GCNNNNNNNGC 3 cut(s) 351, 695, 1192
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 143
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 3 cut(s) 277, 572, 763
NlaIV GGNNCC 2 cut(s) 63, 596
NmeAIII GCCGAG 1 cut(s) 1048
NmuCI GTSAC 2 cut(s) 67, 145
NsiI ATGCAT 1 cut(s) 1229
PceI AGGCCT 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 1104
PcsI WCGNNNNNNNCGW 1 cut(s) 1016
PctI GAATGC 4 cut(s) 240, 830, 1066, 1229
PdiI GCCGGC 1 cut(s) 127
PfeI GAWTC 4 cut(s) 178, 205, 295, 1201
PkrI GCNGC 2 cut(s) 379, 424
PleI GAGTC 3 cut(s) 725, 872, 1240
PpsI GAGTC 3 cut(s) 725, 872, 1240
Psp6I CCWGG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 2 cut(s) 63, 596
PstNI CAGNNNCTG 1 cut(s) 160
PsuI RGATCY 3 cut(s) 61, 563, 613
RsaI GTAC 2 cut(s) 557, 1086
RsaNI GTAC 2 cut(s) 556, 1085
SapI GCTCTTC 1 cut(s) 1104
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 2 cut(s) 378, 423
ScaI AGTACT 2 cut(s) 557, 1086
SchI GAGTC 3 cut(s) 726, 872, 1241
ScrFI CCNGG 2 cut(s) 143, 587
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 4 cut(s) 114, 315, 414, 1236
SfcI CTRYAG 1 cut(s) 1236
SgrAI CRCCGGYG 1 cut(s) 1151
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 3 cut(s) 264, 498, 636
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 4 cut(s) 377, 400, 422, 689
SspMI CTAG 2 cut(s) 261, 641
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 2 cut(s) 141, 585
TaaI ACNGT 1 cut(s) 787
TaqI TCGA 7 cut(s) 48, 108, 120, 330, 876, 1031, 1230
TasI AATT 3 cut(s) 264, 498, 636
TatI WGTACW 2 cut(s) 555, 1084
TauI GCSGC 2 cut(s) 380, 425
TfiI GAWTC 4 cut(s) 178, 205, 295, 1201
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 2 cut(s) 67, 145
Tsp45I GTSAC 2 cut(s) 67, 145
TspDTI ATGAA 3 cut(s) 71, 555, 730
TspGWI ACGGA 1 cut(s) 911
TspRI CASTG 1 cut(s) 817
XapI RAATTY 2 cut(s) 264, 498
XspI CTAG 2 cut(s) 261, 641
ZrmI AGTACT 2 cut(s) 557, 1086
Zsp2I ATGCAT 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.