Rroxscaffold_6G00392340

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001443
Physical Location & Seq
Forward (+)
11895131 .. 11896497
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392340.1

Sequence Viewer

Length: 1353 bp
ATGATTTTGTCGTGGAGGAGAAGAAGGGCCAGCAAGTATGCCAACAGGAAGGAGTTTTCCGGCGAGACTCTAGACATGGGGATGGAGGAGATCGTGATTCCATCCCACTTCCGGTGCCCTATTACTCTGGACCTCATGAAAGATCCGGTCACTTTGTCGACGGGAATGACCTACGACAGGTCCAGCATCGACGCCTGGATCGAGAAAGGGAACAAGACCTGCCCGGTAACCAATCAAGTTCTGGCAACGTTCGACCAAATCCCCAACCACGCGCTACGTAAGATGATCCAAGACTGGTGCGTCGAGAACCGCTCTCACGGCGTGGAACGCATCCCCACGCCTCGGATTCCGGTGTCTGCGCACGAGGTTACTAGAGTTTATCAGAGAATCATGGCCGCGACTCAACGCGGCGATTACAACAAGTGTTTAGAGTTGGTGGCCAAGGTCAGAGGTTGGGGAAAAGACAGCGAGCGTAACAAGAAGTGTATGATGGAGAACAGAATGGGCTGCTGCTTGTCGGCCACTTTCGAGTTCTTCGCCGGAAATGAGGAAACAACGTTGTTGGAGGAGATCTTGGCCGTGCTGCCATGGATGTTTCCCATTGGTGAAGAAGGCCAATCAAAGCTTGGATCTGCAAAATCGTTGAGATGTTTGGGAAAAGATCTCTCTTCAAGGCAAAACGCAGTTTCGGTACTGAGAAAGTTGGTTTCTTTGGATCAAAAACATGTGGATGCGATTATGGAGATTGAAGGAGCCATTGAAGCATTGGTGAAGATAATTAGGGAGGGCCCTAGTGTCTGTCCTACAGCAACTAAAGCTTCTCTATCCTGTATCTTCTACATGATTTCAGGAGCAAGTGATGAAATCAAATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGTTGCTAGAGATGCTTGTTGATGGAGAGAAGAGCCTATGTGAGAGGGCTTTGGGGGTTTTGGAAGGTCTTTGTGATTGCAAGCAAGGGAGAGAAAAGGCCTACGAGAATGCTCTAACCACGCCTCTTCTGGTTAAGAAGATGCTAAGGGTATCAGCCTTGGCGAATGAGTTTTCGGTTTCTATAATTTGGAAGCTCTGCAAGAACCAAAATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGACGAGGAAGAAAATAAGATTTTGATGGAGGCACTTCAAGTGGGCGCATTTGAGAAATTCTTGGTTCTGTTGCAGGTTGGGTGTGGTGATGGAATCAAGGACAAGGTTACTGAGTCGTTGAAGTTGTTCAATCTTCACAGAAGTAAATTAGACTGTGTTGATTCATCCATGGATTTTAAGTATCTGAAGAAAGGCCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

450

Amino Acids

50.32

Weight (kDa)

6.19

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 33 - 104 4.8e-22 U-box domain
ARM_PUB PF25598 122 - 444 1.3e-63 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 2 cut(s) 227, 1213
AccB1I GGYRCC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 158
AccII CGCG 3 cut(s) 272, 398, 408
AciI CCGC 3 cut(s) 310, 396, 408
AclI AACGTT 2 cut(s) 248, 557
AclWI GGATC 5 cut(s) 137, 206, 280, 637, 723
AcoI YGGCCR 4 cut(s) 393, 438, 519, 576
AcsI RAATTY 1 cut(s) 1205
AcyI GRCGYC 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 3 cut(s) 111, 177, 342
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 6 cut(s) 672, 749, 761, 1187, 1270, 1279
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 3 cut(s) 625, 818, 1096
AluI AGCT 3 cut(s) 625, 818, 1096
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 5 cut(s) 137, 206, 280, 637, 723
AoxI GGCC 9 cut(s) 27, 393, 438, 519, 576, 613, 787, 999, 1342
ApaI GGGCCC 1 cut(s) 791
ApeKI GCWGC 3 cut(s) 507, 510, 583
ApoI RAATTY 1 cut(s) 1205
Asp700I GAANNNNTTC 1 cut(s) 1274
AspLEI GCGC 3 cut(s) 274, 361, 1196
AspS9I GGNCC 5 cut(s) 27, 130, 180, 787, 788
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 9 cut(s) 617, 781, 1127, 1133, 1139, 1145, 1151, 1157, 1247
AvaII GGWCC 2 cut(s) 130, 180
BaeGI GKGCMC 2 cut(s) 119, 791
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 791
BauI CACGAG 1 cut(s) 362
BbvI GCAGC 3 cut(s) 494, 497, 570
BceAI ACGGC 2 cut(s) 334, 563
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 4 cut(s) 71, 372, 792, 908
BfmI CTRYAG 1 cut(s) 804
BfuAI ACCTGC 2 cut(s) 227, 1213
BglII AGATCT 2 cut(s) 570, 661
BisI GCNGC 5 cut(s) 396, 409, 508, 511, 584
BlsI GCNGC 5 cut(s) 397, 410, 509, 512, 585
Bme1390I CCNGG 2 cut(s) 196, 224
Bme18I GGWCC 2 cut(s) 130, 180
BmgT120I GGNCC 5 cut(s) 27, 130, 180, 787, 788
BmiI GGNNCC 3 cut(s) 116, 754, 789
BmrFI CCNGG 2 cut(s) 196, 224
BmsI GCATC 5 cut(s) 195, 339, 721, 903, 1032
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu10I CCTNAGC 1 cut(s) 1046
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 5 cut(s) 341, 441, 587, 1059, 1317
BsaWI WCCGGW 3 cut(s) 111, 145, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 177, 342
Bse1I ACTGG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 5 cut(s) 341, 441, 587, 1059, 1317
BseGI GGATG 6 cut(s) 87, 101, 330, 597, 736, 1313
BseLI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BseMII CTCAG 2 cut(s) 686, 1251
BseNI ACTGG 1 cut(s) 299
BseRI GAGGAG 3 cut(s) 31, 101, 581
BseSI GKGCMC 2 cut(s) 119, 791
BseXI GCAGC 3 cut(s) 494, 497, 570
Bsh1236I CGCG 3 cut(s) 272, 398, 408
BshFI GGCC 9 cut(s) 29, 395, 440, 521, 578, 615, 789, 1001, 1344
BshNI GGYRCC 1 cut(s) 114
BsiSI CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
BslI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BsmAI GTCTC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 9 cut(s) 29, 395, 440, 521, 578, 615, 789, 1001, 1344
Bsp120I GGGCCC 1 cut(s) 787
Bsp1286I GDGCHC 2 cut(s) 119, 791
Bsp143I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
Bsp19I CCATGG 2 cut(s) 587, 1317
BspACI CCGC 3 cut(s) 310, 396, 408
BspANI GGCC 9 cut(s) 29, 395, 440, 521, 578, 615, 789, 1001, 1344
BspCNI CTCAG 2 cut(s) 687, 1252
BspFNI CGCG 3 cut(s) 272, 398, 408
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 3 cut(s) 116, 754, 789
BspMI ACCTGC 2 cut(s) 227, 1213
BspPI GGATC 5 cut(s) 137, 206, 280, 637, 723
BspQI GCTCTTC 1 cut(s) 926
BspT107I GGYRCC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 312
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 5 cut(s) 341, 441, 587, 1059, 1317
BssMI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 4 cut(s) 441, 587, 1059, 1317
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 1304
Bst6I CTCTTC 3 cut(s) 673, 926, 1032
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 31, 470, 983
BstDEI CTNAG 3 cut(s) 695, 1046, 1260
BstDSI CCRYGG 2 cut(s) 587, 1317
BstEII GGTNACC 1 cut(s) 226
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 6 cut(s) 87, 101, 330, 597, 736, 1313
BstFNI CGCG 3 cut(s) 272, 398, 408
BstHHI GCGC 3 cut(s) 274, 361, 1196
BstKTI GATC 8 cut(s) 93, 145, 201, 288, 573, 632, 664, 718
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
BstMWI GCNNNNNNNGC 5 cut(s) 318, 327, 761, 815, 913
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 728
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 194, 222
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 2 cut(s) 119, 791
BstSNI TACGTA 1 cut(s) 278
BstUI CGCG 3 cut(s) 272, 398, 408
BstV1I GCAGC 3 cut(s) 494, 497, 570
BstX2I RGATCY 4 cut(s) 142, 570, 629, 661
BstYI RGATCY 4 cut(s) 142, 570, 629, 661
BsuRI GGCC 9 cut(s) 29, 395, 440, 521, 578, 615, 789, 1001, 1344
BtgI CCRYGG 2 cut(s) 587, 1317
BtsCI GGATG 6 cut(s) 87, 101, 330, 597, 736, 1313
BveI ACCTGC 2 cut(s) 227, 1213
Cac8I GCNNGC 3 cut(s) 31, 470, 983
CciI TCATGA 1 cut(s) 135
CfoI GCGC 3 cut(s) 274, 361, 1196
Cfr13I GGNCC 5 cut(s) 27, 130, 180, 787, 788
CseI GACGC 2 cut(s) 200, 289
Csp6I GTAC 1 cut(s) 692
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 7 cut(s) 76, 136, 391, 588, 725, 841, 1318
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 3 cut(s) 695, 1046, 1260
DpnI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 663, 717
DpnII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
DraIII CACNNNGTG 1 cut(s) 322
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 4 cut(s) 393, 438, 519, 576
Eam1104I CTCTTC 3 cut(s) 673, 926, 1032
EarI CTCTTC 3 cut(s) 673, 926, 1032
Eco105I TACGTA 1 cut(s) 278
Eco130I CCWWGG 4 cut(s) 441, 587, 1059, 1317
Eco147I AGGCCT 1 cut(s) 1001
Eco24I GRGCYC 1 cut(s) 791
Eco47I GGWCC 2 cut(s) 130, 180
Eco91I GGTNACC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 787, 788
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 4 cut(s) 441, 587, 1059, 1317
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 4 cut(s) 441, 587, 1059, 1317
FaeI CATG 7 cut(s) 79, 139, 394, 591, 728, 844, 1321
FatI CATG 7 cut(s) 75, 135, 390, 587, 724, 840, 1317
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 5 cut(s) 396, 409, 508, 511, 584
FokI GGATG 6 cut(s) 88, 94, 317, 604, 743, 1300
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 5 cut(s) 396, 409, 508, 511, 584
FspBI CTAG 4 cut(s) 71, 372, 792, 908
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 3 cut(s) 273, 360, 1195
GluI GCNGC 5 cut(s) 396, 409, 508, 511, 584
HaeIII GGCC 9 cut(s) 29, 395, 440, 521, 578, 615, 789, 1001, 1344
HapII CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
HgaI GACGC 2 cut(s) 200, 289
HhaI GCGC 3 cut(s) 274, 361, 1196
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 7 cut(s) 79, 139, 394, 591, 728, 844, 1321
Hin6I GCGC 3 cut(s) 272, 359, 1194
HinP1I GCGC 3 cut(s) 272, 359, 1194
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 2 cut(s) 623, 816
HinfI GANTC 8 cut(s) 67, 97, 346, 387, 400, 1242, 1262, 1310
HpaII CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
HphI GGTGA 9 cut(s) 617, 781, 1127, 1133, 1139, 1145, 1151, 1157, 1247
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 4 cut(s) 345, 384, 449, 1335
Hpy188III TCNNGA 8 cut(s) 71, 94, 128, 136, 202, 304, 849, 873
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 3 cut(s) 163, 194, 305
HpyAV CCTTC 5 cut(s) 18, 43, 605, 743, 959
HpyCH4III ACNGT 1 cut(s) 1304
HpyCH4IV ACGT 3 cut(s) 248, 277, 557
HpyCH4V TGCA 4 cut(s) 635, 981, 1101, 1222
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 327, 761, 815, 913
HpyF3I CTNAG 3 cut(s) 695, 1046, 1260
HpySE526I ACGT 3 cut(s) 248, 277, 557
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 7 cut(s) 79, 139, 394, 591, 728, 844, 1321
HspAI GCGC 3 cut(s) 272, 359, 1194
Kzo9I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
LguI GCTCTTC 1 cut(s) 926
LmnI GCTCC 2 cut(s) 752, 851
Lsp1109I GCAGC 3 cut(s) 494, 497, 570
LweI GCATC 5 cut(s) 195, 339, 721, 903, 1032
MaeI CTAG 4 cut(s) 71, 372, 792, 908
MaeII ACGT 3 cut(s) 248, 277, 557
MaeIII GTNAC 5 cut(s) 148, 226, 367, 473, 1255
MalI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 663, 717
MbiI CCGCTC 1 cut(s) 312
MboI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
MflI RGATCY 4 cut(s) 142, 570, 629, 661
MhlI GDGCHC 2 cut(s) 119, 791
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 6 cut(s) 777, 884, 1086, 1205, 1295, 1346
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 3 cut(s) 61, 394, 1271
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 1274
MscI TGGCCA 1 cut(s) 440
MseI TTAA 2 cut(s) 1035, 1326
MslI CAYNNNNRTG 2 cut(s) 80, 729
Msp20I TGGCCA 1 cut(s) 440
MspI CCGG 6 cut(s) 60, 112, 146, 224, 350, 540
MspR9I CCNGG 2 cut(s) 196, 224
Mva1269I GAATGC 1 cut(s) 1015
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 3 cut(s) 272, 398, 408
MwoI GCNNNNNNNGC 5 cut(s) 318, 327, 761, 815, 913
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 2 cut(s) 587, 1317
NdeII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
NlaIII CATG 7 cut(s) 79, 139, 394, 591, 728, 844, 1321
NlaIV GGNNCC 3 cut(s) 116, 754, 789
NmuCI GTSAC 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 728
PagI TCATGA 1 cut(s) 135
PceI AGGCCT 1 cut(s) 1001
PciI ACATGT 1 cut(s) 724
PciSI GCTCTTC 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 1015
PdmI GAANNNNTTC 1 cut(s) 1274
PfeI GAWTC 5 cut(s) 97, 346, 387, 1242, 1310
PkrI GCNGC 5 cut(s) 397, 410, 509, 512, 585
PleI GAGTC 3 cut(s) 61, 394, 1270
PpsI GAGTC 3 cut(s) 61, 394, 1270
Ppu21I YACGTR 1 cut(s) 278
PscI ACATGT 1 cut(s) 724
Psp1406I AACGTT 2 cut(s) 248, 557
Psp6I CCWGG 1 cut(s) 194
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 3 cut(s) 116, 754, 789
PspOMI GGGCCC 1 cut(s) 787
PspPI GGNCC 5 cut(s) 27, 130, 180, 787, 788
PsuI RGATCY 4 cut(s) 142, 570, 629, 661
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 2 cut(s) 80, 729
SalI GTCGAC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 926
SaqAI TTAA 2 cut(s) 1035, 1326
SatI GCNGC 5 cut(s) 396, 409, 508, 511, 584
Sau3AI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 661, 715
Sau96I GGNCC 5 cut(s) 27, 130, 180, 787, 788
SchI GAGTC 3 cut(s) 61, 394, 1271
ScrFI CCNGG 2 cut(s) 196, 224
SduI GDGCHC 2 cut(s) 119, 791
SfaNI GCATC 5 cut(s) 195, 339, 721, 903, 1032
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 2 cut(s) 130, 180
SmiMI CAYNNNNRTG 2 cut(s) 80, 729
SnaBI TACGTA 1 cut(s) 278
Sse9I AATT 6 cut(s) 777, 884, 1086, 1205, 1295, 1346
SseBI AGGCCT 1 cut(s) 1001
SsiI CCGC 3 cut(s) 310, 396, 408
SspMI CTAG 4 cut(s) 71, 372, 792, 908
StuI AGGCCT 1 cut(s) 1001
StyD4I CCNGG 2 cut(s) 194, 222
StyI CCWWGG 4 cut(s) 441, 587, 1059, 1317
TaaI ACNGT 1 cut(s) 1304
TaiI ACGT 3 cut(s) 251, 280, 560
TaqI TCGA 6 cut(s) 158, 189, 201, 252, 303, 528
TasI AATT 6 cut(s) 777, 884, 1086, 1205, 1295, 1346
TauI GCSGC 2 cut(s) 398, 411
TfiI GAWTC 5 cut(s) 97, 346, 387, 1242, 1310
Tru1I TTAA 2 cut(s) 1035, 1326
Tru9I TTAA 2 cut(s) 1035, 1326
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 507, 510, 583
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 152, 876, 1302
VpaK11BI GGWCC 2 cut(s) 130, 180
XagI CCTNNNNNAGG 1 cut(s) 175
XapI RAATTY 1 cut(s) 1205
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 5 cut(s) 238, 623, 763, 1027, 1115
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 1274
XspI CTAG 4 cut(s) 71, 372, 792, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.