Rh3DG338900

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001443
Physical Location & Seq
Reverse (-)
37872396 .. 37874579
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG338900.1

Sequence Viewer

Length: 1338 bp
ATGATTTTGTCGTGGAGGAGAAGAAGGGCCAGCAAGCATGCCAACAGGAAGGAGTTTTCCGGCGAGACTCTAGACATGGGGATGGAGGAGATCGTGATTCCATCCCACTTCCGTTGCCCTATTACTCTGGACCTCATGAAAGATCCGGTCACCTTGTCGACGGGGATGACCTACGACAGGTCCAGCATCGACGCCTGGATCGAGAAAGGGAACAAGACCTGCCCGGTAACCAATCAAGTTCTGGCAACGTTCGACCAAATCCCCAACCACGCGCTACGTAAGATGATCCAAGACTGGTGCGTCGAGAACCGCTCTCACGGCGTGGAACGCATCCCCACGCCTCGGATTCCTGTGTCTGCGTACGAGGTTACTAGGGTTTGTCAGAGAATCATGGCAGCGACTCAACGCGGCGATTACAACAAGTGTCTAGAGTTGGTGGCCAAGGTCAGAGGTTGGGGAAAAGACAGCGAGTGTAACAAGAAGTGTGTCGTGGAGAACAGAATGGGCTGCTGCTTGTCCGCCACTTTCGAGTTCTTCGCCGGAAATGAGGAAACAACGTTGTTGGAGGAGATCTTGGCCGTGCTGACATGGATGTTTCCGATAGGTGAAGAAGGCCAATCAAAGCTTGGATCTGCAAAATCGTTGAGATGTTTGGTGTGGTTTTTGGAGGGAAAAGATCTCTCTTCAAGGCAAAACGCAGTTTCGGTACTGAGAAAGTTGGTTTCTTTGGATAAAAAACATGTGGATGCGATCATGGAGATTGAAGGAGCCATTGAAGCATTGGTGAAGATAATTAGGGAGGGCCCTAGTGTCTGTCCTACAGCAACTAAAGCTTCTCTATTCTGTGTCTTCTACATGATTTCAGGAGCAAGAGATGAAATCAAATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGTTGCTAGAGATGCTTGTTGATGCAGAGAGGAGCCTATGTGAGAGGGCTTTGGGCGTTTTGGAAGGTCTTTGTGATTGCAAGCAAGGGAAAGAAAAGGCCTACGAGAATGCTCTAACCATGCCTCTTTTGGTTAAGAAGATGCTAAGGGTATCAGCCTTGGCGAATGAGTTTTCGGTTTCTATAATTTGGAAGCTCTGCAAGAACCAAAATGGTGACGGTGACGGTGATGACGAGGAAGAAATTAAGATTTTGATGGAGGCACTTCAAGTGGGCGCATTTAAGAAGTTCTTGGTTCTGTTGCAGGTGGGGTGTGGTGATGGAATCAAGGACAAGGTTACTGAGTTGTTGAAGTTGTTCAATCTTCACATCAGTAAAGTAGACTGTGTTGATTCATCCATGGATTTTAAGTATCTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

445

Amino Acids

49.97

Weight (kDa)

6.97

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 33 - 104 4.7e-22 U-box domain
ARM_PUB PF25598 122 - 443 5.1e-72 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1210
AasI GACNNNNNNGTC 1 cut(s) 299
Acc36I ACCTGC 2 cut(s) 227, 1210
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 158, 1296
AccII CGCG 2 cut(s) 272, 408
AciI CCGC 3 cut(s) 310, 408, 519
AclI AACGTT 2 cut(s) 248, 557
AclWI GGATC 4 cut(s) 137, 206, 280, 637
AcoI YGGCCR 2 cut(s) 438, 576
AcyI GRCGYC 1 cut(s) 192
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 2 cut(s) 362, 708
AfiI CCNNNNNNNGG 2 cut(s) 177, 342
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 6 cut(s) 687, 764, 776, 1184, 1267, 1276
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 3 cut(s) 625, 833, 1111
AluI AGCT 3 cut(s) 625, 833, 1111
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 4 cut(s) 137, 206, 280, 637
AoxI GGCC 6 cut(s) 27, 438, 576, 613, 802, 1014
ApaI GGGCCC 1 cut(s) 806
ApeKI GCWGC 3 cut(s) 395, 507, 510
Asp700I GAANNNNTTC 1 cut(s) 1271
AspLEI GCGC 2 cut(s) 274, 1193
AspS9I GGNCC 5 cut(s) 27, 130, 180, 802, 803
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 7 cut(s) 142, 617, 796, 1142, 1148, 1154, 1244
AvaII GGWCC 2 cut(s) 130, 180
BaeGI GKGCMC 1 cut(s) 806
BalI TGGCCA 1 cut(s) 440
BanII GRGCYC 1 cut(s) 806
BbsI GAAGAC 1 cut(s) 841
BbvI GCAGC 3 cut(s) 407, 494, 497
BccI CCATC 4 cut(s) 76, 109, 1165, 1229
BceAI ACGGC 2 cut(s) 334, 563
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 5 cut(s) 71, 372, 428, 807, 923
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 2 cut(s) 227, 1210
BglII AGATCT 2 cut(s) 570, 676
BisI GCNGC 4 cut(s) 396, 409, 508, 511
BlsI GCNGC 4 cut(s) 397, 410, 509, 512
Bme1390I CCNGG 2 cut(s) 196, 224
Bme18I GGWCC 2 cut(s) 130, 180
BmgT120I GGNCC 5 cut(s) 27, 130, 180, 802, 803
BmiI GGNNCC 3 cut(s) 769, 804, 950
BmrFI CCNGG 2 cut(s) 196, 224
BmsI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
BpiI GAAGAC 1 cut(s) 841
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu10I CCTNAGC 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 341, 441, 1074, 1314
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 342
Bse1I ACTGG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 4 cut(s) 341, 441, 1074, 1314
BseGI GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BseLI CCNNNNNNNGG 2 cut(s) 177, 342
BseMII CTCAG 2 cut(s) 701, 1248
BseNI ACTGG 1 cut(s) 299
BseRI GAGGAG 4 cut(s) 31, 101, 581, 961
BseSI GKGCMC 1 cut(s) 806
BseXI GCAGC 3 cut(s) 407, 494, 497
Bsh1236I CGCG 2 cut(s) 272, 408
BshFI GGCC 6 cut(s) 29, 440, 578, 615, 804, 1016
BsiSI CCGG 4 cut(s) 60, 146, 224, 540
BsiWI CGTACG 1 cut(s) 360
BslI CCNNNNNNNGG 2 cut(s) 177, 342
BsmAI GTCTC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 6 cut(s) 29, 440, 578, 615, 804, 1016
Bsp120I GGGCCC 1 cut(s) 802
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
Bsp19I CCATGG 1 cut(s) 1314
BspACI CCGC 3 cut(s) 310, 408, 519
BspANI GGCC 6 cut(s) 29, 440, 578, 615, 804, 1016
BspCNI CTCAG 2 cut(s) 702, 1249
BspFNI CGCG 2 cut(s) 272, 408
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 3 cut(s) 769, 804, 950
BspMI ACCTGC 2 cut(s) 227, 1210
BspPI GGATC 4 cut(s) 137, 206, 280, 637
BsrBI CCGCTC 1 cut(s) 312
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 4 cut(s) 341, 441, 1074, 1314
BssMI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 441, 1074, 1314
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 3 cut(s) 1136, 1142, 1301
Bst6I CTCTTC 1 cut(s) 688
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 4 cut(s) 31, 35, 39, 998
BstDEI CTNAG 3 cut(s) 710, 1061, 1257
BstDSI CCRYGG 1 cut(s) 1314
BstEII GGTNACC 2 cut(s) 148, 226
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BstFNI CGCG 2 cut(s) 272, 408
BstHHI GCGC 2 cut(s) 274, 1193
BstKTI GATC 8 cut(s) 93, 145, 201, 288, 573, 632, 679, 753
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
BstMWI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 2 cut(s) 41, 743
BstPI GGTNACC 2 cut(s) 148, 226
BstSCI CCNGG 2 cut(s) 194, 222
BstSFI CTRYAG 1 cut(s) 819
BstSLI GKGCMC 1 cut(s) 806
BstSNI TACGTA 1 cut(s) 278
BstUI CGCG 2 cut(s) 272, 408
BstV1I GCAGC 3 cut(s) 407, 494, 497
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 4 cut(s) 142, 570, 629, 676
BstYI RGATCY 4 cut(s) 142, 570, 629, 676
BsuRI GGCC 6 cut(s) 29, 440, 578, 615, 804, 1016
BtgI CCRYGG 1 cut(s) 1314
BtsCI GGATG 7 cut(s) 87, 101, 171, 330, 597, 751, 1310
BveI ACCTGC 2 cut(s) 227, 1210
Cac8I GCNNGC 4 cut(s) 31, 35, 39, 998
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 274, 1193
Cfr13I GGNCC 5 cut(s) 27, 130, 180, 802, 803
CseI GACGC 2 cut(s) 200, 289
Csp6I GTAC 2 cut(s) 361, 707
CspCI CAANNNNNGTGG 4 cut(s) 95, 130, 876, 911
CviQI GTAC 2 cut(s) 361, 707
DdeI CTNAG 3 cut(s) 710, 1061, 1257
DpnI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 678, 752
DpnII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
DraIII CACNNNGTG 1 cut(s) 322
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 2 cut(s) 438, 576
Eam1104I CTCTTC 1 cut(s) 688
EarI CTCTTC 1 cut(s) 688
EciI GGCGGA 1 cut(s) 508
Eco105I TACGTA 1 cut(s) 278
Eco130I CCWWGG 3 cut(s) 441, 1074, 1314
Eco147I AGGCCT 1 cut(s) 1016
Eco24I GRGCYC 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 130, 180
Eco91I GGTNACC 2 cut(s) 148, 226
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 802, 803
EcoO65I GGTNACC 2 cut(s) 148, 226
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 3 cut(s) 441, 1074, 1314
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 3 cut(s) 441, 1074, 1314
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FblI GTMKAC 2 cut(s) 158, 1296
Fnu4HI GCNGC 4 cut(s) 396, 409, 508, 511
FokI GGATG 7 cut(s) 88, 94, 178, 317, 604, 758, 1297
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 4 cut(s) 396, 409, 508, 511
FspBI CTAG 5 cut(s) 71, 372, 428, 807, 923
GlaI GCGC 2 cut(s) 273, 1192
GluI GCNGC 4 cut(s) 396, 409, 508, 511
HaeIII GGCC 6 cut(s) 29, 440, 578, 615, 804, 1016
HapII CCGG 4 cut(s) 60, 146, 224, 540
HgaI GACGC 2 cut(s) 200, 289
HhaI GCGC 2 cut(s) 274, 1193
Hin1I GRCGYC 1 cut(s) 192
Hin6I GCGC 2 cut(s) 272, 1191
HinP1I GCGC 2 cut(s) 272, 1191
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 2 cut(s) 623, 831
HinfI GANTC 7 cut(s) 67, 97, 346, 387, 400, 1239, 1307
HpaII CCGG 4 cut(s) 60, 146, 224, 540
HphI GGTGA 7 cut(s) 142, 617, 796, 1142, 1148, 1154, 1244
Hpy166II GTNNAC 2 cut(s) 159, 1297
Hpy188I TCNGA 5 cut(s) 345, 384, 449, 600, 1332
Hpy188III TCNNGA 9 cut(s) 71, 94, 128, 136, 202, 304, 428, 864, 888
Hpy8I GTNNAC 2 cut(s) 159, 1297
Hpy99I CGWCG 3 cut(s) 163, 194, 305
HpyAV CCTTC 5 cut(s) 18, 43, 605, 758, 974
HpyCH4III ACNGT 3 cut(s) 1136, 1142, 1301
HpyCH4IV ACGT 3 cut(s) 248, 277, 557
HpyCH4V TGCA 5 cut(s) 635, 941, 996, 1116, 1219
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
HpyF3I CTNAG 3 cut(s) 710, 1061, 1257
HpySE526I ACGT 3 cut(s) 248, 277, 557
Hsp92I GRCGYC 1 cut(s) 192
HspAI GCGC 2 cut(s) 272, 1191
Kzo9I GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
LmnI GCTCC 3 cut(s) 767, 866, 948
Lsp1109I GCAGC 3 cut(s) 407, 494, 497
LweI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
MaeI CTAG 5 cut(s) 71, 372, 428, 807, 923
MaeII ACGT 3 cut(s) 248, 277, 557
MaeIII GTNAC 7 cut(s) 148, 226, 367, 473, 1130, 1136, 1252
MalI GATC 8 cut(s) 92, 144, 200, 287, 572, 631, 678, 752
MbiI CCGCTC 1 cut(s) 312
MboI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
MboII GAAGA 9 cut(s) 33, 526, 620, 675, 799, 841, 1066, 1166, 1271
MflI RGATCY 4 cut(s) 142, 570, 629, 676
MhlI GDGCHC 1 cut(s) 806
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 4 cut(s) 792, 899, 1101, 1158
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 2 cut(s) 61, 394
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 440
MseI TTAA 4 cut(s) 1050, 1161, 1197, 1323
MslI CAYNNNNRTG 2 cut(s) 80, 744
Msp20I TGGCCA 1 cut(s) 440
MspI CCGG 4 cut(s) 60, 146, 224, 540
MspR9I CCNGG 2 cut(s) 196, 224
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 2 cut(s) 272, 408
MwoI GCNNNNNNNGC 5 cut(s) 318, 327, 776, 830, 928
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 1314
NdeII GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
NlaIV GGNNCC 3 cut(s) 769, 804, 950
NmuCI GTSAC 3 cut(s) 148, 1130, 1136
NspI RCATGY 2 cut(s) 41, 743
PaeI GCATGC 1 cut(s) 41
PagI TCATGA 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 1210
PceI AGGCCT 1 cut(s) 1016
PciI ACATGT 1 cut(s) 739
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 1146
PctI GAATGC 1 cut(s) 1030
PdmI GAANNNNTTC 1 cut(s) 1271
PfeI GAWTC 5 cut(s) 97, 346, 387, 1239, 1307
Pfl23II CGTACG 1 cut(s) 360
PkrI GCNGC 4 cut(s) 397, 410, 509, 512
PleI GAGTC 2 cut(s) 61, 394
PpsI GAGTC 2 cut(s) 61, 394
Ppu21I YACGTR 1 cut(s) 278
PscI ACATGT 1 cut(s) 739
Psp1406I AACGTT 2 cut(s) 248, 557
Psp6I CCWGG 1 cut(s) 194
PspEI GGTNACC 2 cut(s) 148, 226
PspGI CCWGG 1 cut(s) 194
PspLI CGTACG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 769, 804, 950
PspOMI GGGCCC 1 cut(s) 802
PspPI GGNCC 5 cut(s) 27, 130, 180, 802, 803
PsuI RGATCY 4 cut(s) 142, 570, 629, 676
RsaI GTAC 2 cut(s) 362, 708
RsaNI GTAC 2 cut(s) 361, 707
RseI CAYNNNNRTG 2 cut(s) 80, 744
SalI GTCGAC 1 cut(s) 157
SaqAI TTAA 4 cut(s) 1050, 1161, 1197, 1323
SatI GCNGC 4 cut(s) 396, 409, 508, 511
Sau3AI GATC 8 cut(s) 90, 142, 198, 285, 570, 629, 676, 750
Sau96I GGNCC 5 cut(s) 27, 130, 180, 802, 803
SchI GAGTC 2 cut(s) 61, 394
ScrFI CCNGG 2 cut(s) 196, 224
SduI GDGCHC 1 cut(s) 806
SfaNI GCATC 6 cut(s) 195, 339, 736, 918, 928, 1047
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 2 cut(s) 130, 180
SmiMI CAYNNNNRTG 2 cut(s) 80, 744
SnaBI TACGTA 1 cut(s) 278
SphI GCATGC 1 cut(s) 41
Sse9I AATT 4 cut(s) 792, 899, 1101, 1158
SseBI AGGCCT 1 cut(s) 1016
SsiI CCGC 3 cut(s) 310, 408, 519
SspMI CTAG 5 cut(s) 71, 372, 428, 807, 923
StuI AGGCCT 1 cut(s) 1016
StyD4I CCNGG 2 cut(s) 194, 222
StyI CCWWGG 3 cut(s) 441, 1074, 1314
TaaI ACNGT 3 cut(s) 1136, 1142, 1301
TaiI ACGT 3 cut(s) 251, 280, 560
TaqI TCGA 6 cut(s) 158, 189, 201, 252, 303, 528
TasI AATT 4 cut(s) 792, 899, 1101, 1158
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 5 cut(s) 97, 346, 387, 1239, 1307
Tru1I TTAA 4 cut(s) 1050, 1161, 1197, 1323
Tru9I TTAA 4 cut(s) 1050, 1161, 1197, 1323
TseFI GTSAC 3 cut(s) 148, 1130, 1136
TseI GCWGC 3 cut(s) 395, 507, 510
Tsp45I GTSAC 3 cut(s) 148, 1130, 1136
TspDTI ATGAA 3 cut(s) 152, 891, 1299
TspGWI ACGGA 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 130, 180
XagI CCTNNNNNAGG 1 cut(s) 175
XbaI TCTAGA 2 cut(s) 70, 427
XceI RCATGY 2 cut(s) 41, 743
XcmI CCANNNNNNNNNTGG 4 cut(s) 238, 623, 778, 1042
XmiI GTMKAC 2 cut(s) 158, 1296
XmnI GAANNNNTTC 1 cut(s) 1271
XspI CTAG 5 cut(s) 71, 372, 428, 807, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.