MD09G1266300.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001443
Physical Location & Seq
Forward (+)
34079681 .. 34090119
10439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1266300.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGACGTGGGGGAGATCGCGATTCCGACGCATTTCTGGTTCCTGATTTCCCTCGACTTGATGAAAGATCCGGTGACACTTTCGACGGGAATCACGTACGACAGGTCAAGCATCGAGAAGTGGATCGAGACTGGCAACAATACTTGTTCTGTGACTAATCAGGAGCTCATGAGTTTTGACCAGATTCTGAACCATGCACTATGTCGGATTATCCAAGATTGGTGCGTGGAGAATAAGTCTTATGGCATCGAGCGAATTCCAATGCCACGGATCCTTGTGATTCCATACGAGGTGTCGAGGATTCGTGAGAGAATCATGGGAGCAGCACGGCATTTGAATGAGAAGAAGTGTCGAGAACTGGTGGGGAAGATTAAAGAGTGGGAAAGTGAGCGGAACAGGAAGTGCATGGCGGAAAACGGAATTGGCGGCTGCTTATCAGCTGTGTTCGAGTCCTTTACCGGCAGTTCAGCAAACGAATATGTTGAGATATTGGTGGAAATTTTAACAGTGCTCCCGTGGATGTTTCCATTGAGTGAAGAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

182

Amino Acids

20.79

Weight (kDa)

5.08

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 8 - 79 5.2e-14 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 391
AccII CGCG 1 cut(s) 20
AciI CCGC 3 cut(s) 391, 410, 426
AclWI GGATC 4 cut(s) 62, 131, 265, 278
AcsI RAATTY 2 cut(s) 255, 498
AfaI GTAC 1 cut(s) 98
AgsI TTSAA 1 cut(s) 337
AjiI CACGTC 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
AluBI AGCT 2 cut(s) 166, 440
AluI AGCT 2 cut(s) 166, 440
Alw21I GWGCWC 2 cut(s) 168, 513
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 4 cut(s) 62, 131, 265, 278
AlwNI CAGNNNCTG 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 323, 429
ApoI RAATTY 2 cut(s) 255, 498
AsuHPI GGTGA 1 cut(s) 85
BamHI GGATCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 168, 513
BbvI GCAGC 2 cut(s) 335, 416
BceAI ACGGC 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 122
BisI GCNGC 3 cut(s) 324, 427, 430
BlsI GCNGC 3 cut(s) 325, 428, 431
BmgBI CACGTC 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 41, 272
BmsI GCATC 2 cut(s) 120, 255
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 266, 515
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bse118I RCCGGY 1 cut(s) 458
Bse1I ACTGG 2 cut(s) 136, 363
BseDI CCNNGG 2 cut(s) 266, 515
BseGI GGATG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 136, 363
BseXI GCAGC 2 cut(s) 335, 416
Bsh1236I CGCG 1 cut(s) 20
BsiHKAI GWGCWC 2 cut(s) 168, 513
BsiSI CCGG 2 cut(s) 71, 459
BsiWI CGTACG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 168, 513
Bsp143I GATC 4 cut(s) 15, 67, 123, 270
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 3 cut(s) 391, 410, 426
BspFNI CGCG 1 cut(s) 20
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 41, 272
BspPI GGATC 4 cut(s) 62, 131, 265, 278
BsrBI CCGCTC 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 458
BsrI ACTGG 2 cut(s) 136, 363
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 266, 515
BssMI GATC 4 cut(s) 15, 67, 123, 270
Bst4CI ACNGT 1 cut(s) 508
BstBAI YACGTR 1 cut(s) 96
BstDSI CCRYGG 2 cut(s) 266, 515
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 4 cut(s) 18, 70, 126, 273
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 4 cut(s) 15, 67, 123, 270
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 335, 416
BstX2I RGATCY 2 cut(s) 67, 270
BstYI RGATCY 2 cut(s) 67, 270
BtgI CCRYGG 2 cut(s) 266, 515
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 513
BtuMI TCGCGA 1 cut(s) 20
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 458
CseI GACGC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 97
CviAII CATG 4 cut(s) 169, 194, 316, 406
CviJI RGCY 3 cut(s) 166, 429, 440
CviKI_1 RGCY 3 cut(s) 166, 429, 440
CviQI GTAC 1 cut(s) 97
DpnI GATC 4 cut(s) 17, 69, 125, 272
DpnII GATC 4 cut(s) 15, 67, 123, 270
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 4 cut(s) 172, 197, 319, 409
FaiI YATR 8 cut(s) 170, 195, 202, 243, 286, 317, 407, 480
FatI CATG 4 cut(s) 168, 193, 315, 405
Fnu4HI GCNGC 3 cut(s) 324, 427, 430
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 324, 427, 430
GluI GCNGC 3 cut(s) 324, 427, 430
HapII CCGG 2 cut(s) 71, 459
HgaI GACGC 1 cut(s) 37
Hin1II CATG 4 cut(s) 172, 197, 319, 409
HinfI GANTC 7 cut(s) 22, 90, 184, 280, 301, 312, 449
HpaII CCGG 2 cut(s) 71, 459
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 27, 189, 207
Hpy188III TCNNGA 8 cut(s) 19, 43, 115, 127, 161, 169, 305, 353
Hpy99I CGWCG 2 cut(s) 31, 88
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 2 cut(s) 6, 95
HpyCH4V TGCA 2 cut(s) 197, 405
HpySE526I ACGT 2 cut(s) 6, 95
Hsp92II CATG 4 cut(s) 172, 197, 319, 409
Kzo9I GATC 4 cut(s) 15, 67, 123, 270
LmnI GCTCC 3 cut(s) 163, 320, 516
Lsp1109I GCAGC 2 cut(s) 335, 416
LweI GCATC 2 cut(s) 120, 255
MaeII ACGT 2 cut(s) 6, 95
MaeIII GTNAC 2 cut(s) 73, 151
MalI GATC 4 cut(s) 17, 69, 125, 272
MbiI CCGCTC 1 cut(s) 391
MboI GATC 4 cut(s) 15, 67, 123, 270
MboII GAAGA 2 cut(s) 355, 379
MflI RGATCY 2 cut(s) 67, 270
MhlI GDGCHC 2 cut(s) 168, 513
MluCI AATT 3 cut(s) 255, 420, 498
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 2 cut(s) 50, 185
MnlI CCTC 3 cut(s) 62, 283, 291
MseI TTAA 2 cut(s) 372, 503
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 2 cut(s) 71, 459
MvnI CGCG 1 cut(s) 20
NdeII GATC 4 cut(s) 15, 67, 123, 270
NlaIII CATG 4 cut(s) 172, 197, 319, 409
NlaIV GGNNCC 2 cut(s) 41, 272
NmuCI GTSAC 2 cut(s) 73, 151
NruI TCGCGA 1 cut(s) 20
PagI TCATGA 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 6 cut(s) 22, 90, 184, 280, 301, 312
Pfl23II CGTACG 1 cut(s) 96
PkrI GCNGC 3 cut(s) 325, 428, 431
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 168
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 41, 272
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 2 cut(s) 67, 270
PvuII CAGCTG 1 cut(s) 440
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 372, 503
SatI GCNGC 3 cut(s) 324, 427, 430
Sau3AI GATC 4 cut(s) 15, 67, 123, 270
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 2 cut(s) 168, 513
SetI ASST 6 cut(s) 9, 98, 107, 168, 294, 442
SfaNI GCATC 2 cut(s) 120, 255
SmiMI CAYNNNNRTG 1 cut(s) 336
Sse9I AATT 3 cut(s) 255, 420, 498
SsiI CCGC 3 cut(s) 391, 410, 426
SstI GAGCTC 1 cut(s) 168
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 2 cut(s) 9, 98
TaqI TCGA 8 cut(s) 54, 83, 114, 126, 249, 296, 352, 447
TasI AATT 3 cut(s) 255, 420, 498
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 6 cut(s) 22, 90, 184, 280, 301, 312
Tru1I TTAA 2 cut(s) 372, 503
Tru9I TTAA 2 cut(s) 372, 503
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 2 cut(s) 73, 151
TseI GCWGC 2 cut(s) 323, 429
Tsp45I GTSAC 2 cut(s) 73, 151
TspDTI ATGAA 1 cut(s) 77
TspGWI ACGGA 2 cut(s) 283, 432
TspRI CASTG 1 cut(s) 513
XapI RAATTY 2 cut(s) 255, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.