Prupe.3G164200_v2.0.a1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001443
Physical Location & Seq
Forward (+)
18337853 .. 18339197
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G164200.1

Sequence Viewer

Length: 1344 bp
ATGATTTTGTCTTGGAGAAGAAGAAAATCTACCCGCCACGCCAACAAGAAGAAGGAGTTTTTGGCTGGCGAGAATTTGGACATGGAGATGGAGGAGATCGCGGTTCCCACGCATTTTCGATGCCCGATATCTCTCGATTTGATGAAGGATCCGGTGACGCTGTCGACGGGGATGACGTACGACCGATCAAGCATTGAGAAGTGGATAGAGACCGGGAACAGGACATGCCCGGCAACTAACCAGGAACTCAAGAGTTTTGACCAAATACCAAACCATGCACTACGTCGAATGATCCAAGATTGGTGCGTCGAAAACCGGTCTTATGGGATCGAACGGATCCCAACGCCTCGAATCCCCGTGACAAAGTACGAGGTTTCGAGGATTTGTGAGAGAATTGCCGCCGCGGCACGACTCGGGGACGGTAGAAAGTGTGGAGAATTGGTGGGGAAGATCAAAGCATGGGGCAAAGAGAGCGAGAGGAACAAGAAGTGTATGGTGGAGAATGGAATTGGCTGCTGCTTGTCAGCTGCGTTAGATTCTTTTGCCAGCATTTCAATGGAGGATCATGTGGAGATATTGTTGGAGATTTTGTCAATGCTGCCATGGATGTTTCCAGTGAGTGAAGAAGGCCAATCAAAGCTTGGATCTGCAGGGTCTTTGCGATGTTTCTTGTCGTTTTTGGAGGGCAAAGATCTATCAGCAAGGCAAAACGCAGTTTTGGTACTGGGAAAGTTGCTTTCTTTGGATCAAAAACATGTGGATGACTTTGTTGAGAGTGAAGGAGCAATTGAAGCATTGGTTGGGATAATTAGAGAGCCTATTTCTCCTGCAGCTACTAAGGCTGCTCTGACTTCTGTTTTGTTCATGATTTCATTTCCATCAGCAGCCAGTGATGAAATCAGATCAAGATTTGTGGAGTTGGGTTTGGTTTCTTTGCTGCTAGAGATTATTGTTGACGCAGAGAGACGCCTGTGTGAGAGGGCTTTGGGAGTTTTGGAGGGTCTTTGTGATTGCAAACAAGGGAAAGACAAGGTCCTTGAGAATGCTCTAACAATACCCCTTTTGGTTAAGAAGATTTTGAGGATTTCGGAGTTGGCGACTGAGTTTTCGATCGCTATAATTTGGAAGCTCTGCAGAATTGAAACTGAGGAAAGCGTTTTGATTGAGGCACTTCAAGTGGGTGCATTTCAGAAGCTCTTGGTTCTCTTGCAGGTTGGTTGTGGTGATGGGATAAAGGAGAAGGTTACTGACTTGTTGAAATTGTTCAATCTTCACTCAAGTAAAATGGACTGTGTTAATTCATCCATGGATTTCAAGAACCTAAAGAGGCCATTTTTGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

448

Amino Acids

49.89

Weight (kDa)

6.64

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1201
AccI GTMKAC 1 cut(s) 164
AccII CGCG 2 cut(s) 101, 404
AciI CCGC 5 cut(s) 34, 101, 399, 402, 404
AclWI GGATC 9 cut(s) 143, 156, 286, 331, 335, 344, 570, 652, 753
AcsI RAATTY 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 967
AfaI GTAC 3 cut(s) 179, 368, 723
AfiI CCNNNNNNNGG 2 cut(s) 219, 1337
AflIII ACRYGT 1 cut(s) 754
AgeI ACCGGT 1 cut(s) 315
AgsI TTSAA 7 cut(s) 555, 791, 1142, 1175, 1258, 1267, 1315
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 4 cut(s) 44, 76, 783, 815
AluBI AGCT 5 cut(s) 527, 640, 833, 1129, 1195
AluI AGCT 5 cut(s) 527, 640, 833, 1129, 1195
Alw26I GTCTC 2 cut(s) 203, 958
AlwI GGATC 9 cut(s) 143, 156, 286, 331, 335, 344, 570, 652, 753
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 2 cut(s) 628, 1328
ApeKI GCWGC 8 cut(s) 513, 516, 527, 598, 830, 842, 884, 937
ApoI RAATTY 1 cut(s) 73
ArsI GACNNNNNNTTYG 4 cut(s) 841, 873, 1090, 1122
AsiGI ACCGGT 1 cut(s) 315
Asp700I GAANNNNTTC 1 cut(s) 1262
AspS9I GGNCC 1 cut(s) 1033
AsuC2I CCSGG 2 cut(s) 214, 230
AsuHPI GGTGA 2 cut(s) 166, 1235
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 1033
BamHI GGATCC 2 cut(s) 148, 336
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
BbvI GCAGC 8 cut(s) 500, 503, 514, 585, 829, 842, 896, 924
BccI CCATC 3 cut(s) 82, 886, 1220
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 2 cut(s) 214, 230
BcoDI GTCTC 2 cut(s) 203, 958
BfaI CTAG 1 cut(s) 941
BfmI CTRYAG 3 cut(s) 648, 828, 1132
BfuAI ACCTGC 1 cut(s) 1201
BglI GCCNNNNNGGC 1 cut(s) 404
BglII AGATCT 1 cut(s) 691
Bme1390I CCNGG 3 cut(s) 214, 230, 242
Bme18I GGWCC 1 cut(s) 1033
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 1033
BmiI GGNNCC 3 cut(s) 105, 150, 338
BmrFI CCNGG 3 cut(s) 214, 230, 242
BmrI ACTGGG 1 cut(s) 734
BmsI GCATC 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 734
BpuEI CTTGAG 3 cut(s) 233, 1058, 1261
BpuMI CCSGG 2 cut(s) 214, 230
BsaHI GRCGYC 1 cut(s) 967
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 402, 602, 1305
BsaWI WCCGGW 2 cut(s) 151, 315
BsaXI ACNNNNNCTCC 6 cut(s) 426, 456, 563, 575, 593, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 1337
Bse118I RCCGGY 1 cut(s) 315
Bse1I ACTGG 3 cut(s) 614, 729, 888
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 3 cut(s) 402, 602, 1305
BseGI GGATG 4 cut(s) 177, 612, 766, 1301
BseLI CCNNNNNNNGG 2 cut(s) 219, 1337
BseMII CTCAG 2 cut(s) 1092, 1137
BseNI ACTGG 3 cut(s) 614, 729, 888
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 8 cut(s) 500, 503, 514, 585, 829, 842, 896, 924
Bsh1236I CGCG 2 cut(s) 101, 404
Bsh1285I CGRYCG 2 cut(s) 184, 1113
BshFI GGCC 2 cut(s) 630, 1330
BshTI ACCGGT 1 cut(s) 315
BsiEI CGRYCG 2 cut(s) 184, 1113
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 4 cut(s) 152, 213, 230, 316
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 431
BslI CCNNNNNNNGG 2 cut(s) 219, 1337
BsmAI GTCTC 2 cut(s) 203, 958
BsmBI CGTCTC 1 cut(s) 958
BsmFI GGGAC 1 cut(s) 431
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 2 cut(s) 630, 1330
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 413
Bsp19I CCATGG 2 cut(s) 602, 1305
BspACI CCGC 5 cut(s) 34, 101, 399, 402, 404
BspANI GGCC 2 cut(s) 630, 1330
BspCNI CTCAG 2 cut(s) 1093, 1138
BspFNI CGCG 2 cut(s) 101, 404
BspHI TCATGA 1 cut(s) 864
BspLI GGNNCC 3 cut(s) 105, 150, 338
BspMAI CTGCAG 3 cut(s) 652, 832, 1136
BspMI ACCTGC 1 cut(s) 1201
BspPI GGATC 9 cut(s) 143, 156, 286, 331, 335, 344, 570, 652, 753
BspTNI GGTCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 315
BsrI ACTGG 3 cut(s) 614, 729, 888
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 402, 602, 1305
BssNI GRCGYC 1 cut(s) 967
BssT1I CCWWGG 2 cut(s) 602, 1305
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 422, 1292
BstACI GRCGYC 1 cut(s) 967
BstC8I GCNNGC 2 cut(s) 67, 547
BstDEI CTNAG 3 cut(s) 837, 1101, 1146
BstDSI CCRYGG 3 cut(s) 402, 602, 1305
BstF5I GGATG 4 cut(s) 177, 612, 766, 1301
BstFNI CGCG 2 cut(s) 101, 404
BstMAI GTCTC 2 cut(s) 203, 958
BstMCI CGRYCG 2 cut(s) 184, 1113
BstMWI GCNNNNNNNGC 4 cut(s) 404, 471, 791, 839
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 2 cut(s) 228, 758
BstSCI CCNGG 3 cut(s) 212, 228, 240
BstSFI CTRYAG 3 cut(s) 648, 828, 1132
BstUI CGCG 2 cut(s) 101, 404
BstV1I GCAGC 8 cut(s) 500, 503, 514, 585, 829, 842, 896, 924
BstX2I RGATCY 4 cut(s) 148, 336, 644, 691
BstYI RGATCY 4 cut(s) 148, 336, 644, 691
BsuRI GGCC 2 cut(s) 630, 1330
BtgI CCRYGG 3 cut(s) 402, 602, 1305
BtgZI GCGATG 1 cut(s) 676
BtsCI GGATG 4 cut(s) 177, 612, 766, 1301
BtsIMutI CAGTG 2 cut(s) 621, 895
BveI ACCTGC 1 cut(s) 1201
Cac8I GCNNGC 2 cut(s) 67, 547
CciI TCATGA 1 cut(s) 864
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 1033
Cfr42I CCGCGG 1 cut(s) 405
CseI GACGC 4 cut(s) 166, 295, 965, 975
Csp6I GTAC 3 cut(s) 178, 367, 722
CspAI ACCGGT 1 cut(s) 315
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
CviAII CATG 9 cut(s) 82, 225, 275, 459, 566, 603, 755, 865, 1306
CviQI GTAC 3 cut(s) 178, 367, 722
DdeI CTNAG 3 cut(s) 837, 1101, 1146
Eco130I CCWWGG 2 cut(s) 602, 1305
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 1033
Eco88I CYCGRG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 1033
EcoRII CCWGG 1 cut(s) 240
EcoRV GATATC 1 cut(s) 129
EcoT14I CCWWGG 2 cut(s) 602, 1305
ErhI CCWWGG 2 cut(s) 602, 1305
Esp3I CGTCTC 1 cut(s) 958
FaeI CATG 9 cut(s) 85, 228, 278, 462, 569, 606, 758, 868, 1309
FaqI GGGAC 1 cut(s) 431
FatI CATG 9 cut(s) 81, 224, 274, 458, 565, 602, 754, 864, 1305
FauI CCCGC 1 cut(s) 41
FblI GTMKAC 1 cut(s) 164
FokI GGATG 4 cut(s) 184, 619, 773, 1288
FspBI CTAG 1 cut(s) 941
HaeIII GGCC 2 cut(s) 630, 1330
HapII CCGG 4 cut(s) 152, 213, 230, 316
HgaI GACGC 4 cut(s) 166, 295, 965, 975
Hin1I GRCGYC 1 cut(s) 967
Hin1II CATG 9 cut(s) 85, 228, 278, 462, 569, 606, 758, 868, 1309
HincII GTYRAC 2 cut(s) 165, 955
HindII GTYRAC 2 cut(s) 165, 955
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 3 cut(s) 351, 411, 536
HpaII CCGG 4 cut(s) 152, 213, 230, 316
HphI GGTGA 2 cut(s) 166, 1235
Hpy166II GTNNAC 2 cut(s) 165, 955
Hpy188I TCNGA 4 cut(s) 849, 902, 1090, 1191
Hpy188III TCNNGA 5 cut(s) 134, 250, 865, 906, 1315
Hpy8I GTNNAC 2 cut(s) 165, 955
Hpy99I CGWCG 3 cut(s) 169, 288, 311
HpyAV CCTTC 5 cut(s) 46, 139, 620, 773, 1234
HpyCH4III ACNGT 2 cut(s) 422, 1292
HpyCH4IV ACGT 2 cut(s) 176, 283
HpyCH4V TGCA 7 cut(s) 278, 650, 830, 1014, 1134, 1184, 1210
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 471, 791, 839
HpyF3I CTNAG 3 cut(s) 837, 1101, 1146
HpySE526I ACGT 2 cut(s) 176, 283
Hsp92I GRCGYC 1 cut(s) 967
Hsp92II CATG 9 cut(s) 85, 228, 278, 462, 569, 606, 758, 868, 1309
KspI CCGCGG 1 cut(s) 405
LmnI GCTCC 1 cut(s) 782
Lsp1109I GCAGC 8 cut(s) 500, 503, 514, 585, 829, 842, 896, 924
LweI GCATC 1 cut(s) 110
MaeI CTAG 1 cut(s) 941
MaeII ACGT 2 cut(s) 176, 283
MaeIII GTNAC 3 cut(s) 154, 358, 1243
MboII GAAGA 7 cut(s) 30, 33, 61, 460, 635, 1084, 1262
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 4 cut(s) 148, 336, 644, 691
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 1262
MseI TTAA 2 cut(s) 1068, 1296
MslI CAYNNNNRTG 3 cut(s) 86, 554, 759
MspA1I CMGCKG 2 cut(s) 404, 527
MspI CCGG 4 cut(s) 152, 213, 230, 316
MspR9I CCNGG 3 cut(s) 214, 230, 242
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 2 cut(s) 101, 404
MwoI GCNNNNNNNGC 4 cut(s) 404, 471, 791, 839
NciI CCSGG 2 cut(s) 214, 230
NcoI CCATGG 2 cut(s) 602, 1305
NlaIII CATG 9 cut(s) 85, 228, 278, 462, 569, 606, 758, 868, 1309
NlaIV GGNNCC 3 cut(s) 105, 150, 338
NmuCI GTSAC 2 cut(s) 154, 358
NspI RCATGY 2 cut(s) 228, 758
PagI TCATGA 1 cut(s) 864
PciI ACATGT 1 cut(s) 754
PcsI WCGNNNNNNNCGW 2 cut(s) 173, 1094
PctI GAATGC 1 cut(s) 1048
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 2 cut(s) 351, 536
Pfl23II CGTACG 1 cut(s) 177
PflFI GACNNNGTC 2 cut(s) 160, 1031
PinAI ACCGGT 1 cut(s) 315
Ple19I CGATCG 1 cut(s) 1113
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PpuMI RGGWCCY 1 cut(s) 1033
PscI ACATGT 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 105, 150, 338
PspPI GGNCC 1 cut(s) 1033
PspPPI RGGWCCY 1 cut(s) 1033
PstI CTGCAG 3 cut(s) 652, 832, 1136
PsuI RGATCY 4 cut(s) 148, 336, 644, 691
PsyI GACNNNGTC 2 cut(s) 160, 1031
PvuI CGATCG 1 cut(s) 1113
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 3 cut(s) 179, 368, 723
RsaNI GTAC 3 cut(s) 178, 367, 722
RseI CAYNNNNRTG 3 cut(s) 86, 554, 759
SacII CCGCGG 1 cut(s) 405
SalI GTCGAC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 1068, 1296
Sau96I GGNCC 1 cut(s) 1033
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 3 cut(s) 214, 230, 242
SfaNI GCATC 1 cut(s) 110
SfcI CTRYAG 3 cut(s) 648, 828, 1132
Sfr303I CCGCGG 1 cut(s) 405
SgrBI CCGCGG 1 cut(s) 405
SinI GGWCC 1 cut(s) 1033
SmiMI CAYNNNNRTG 3 cut(s) 86, 554, 759
SmlI CTYRAG 3 cut(s) 248, 1037, 1276
SmoI CTYRAG 3 cut(s) 248, 1037, 1276
SsiI CCGC 5 cut(s) 34, 101, 399, 402, 404
SspMI CTAG 1 cut(s) 941
StyD4I CCNGG 3 cut(s) 212, 228, 240
StyI CCWWGG 2 cut(s) 602, 1305
TaaI ACNGT 2 cut(s) 422, 1292
TaiI ACGT 2 cut(s) 179, 286
TaqI TCGA 9 cut(s) 118, 135, 164, 286, 309, 330, 349, 377, 1109
TaqII GACCGA 1 cut(s) 198
TauI GCSGC 3 cut(s) 401, 404, 407
TfiI GAWTC 2 cut(s) 351, 536
Tru1I TTAA 2 cut(s) 1068, 1296
Tru9I TTAA 2 cut(s) 1068, 1296
TscAI CASTG 2 cut(s) 621, 895
TseFI GTSAC 2 cut(s) 154, 358
TseI GCWGC 8 cut(s) 513, 516, 527, 598, 830, 842, 884, 937
Tsp45I GTSAC 2 cut(s) 154, 358
TspDTI ATGAA 5 cut(s) 158, 853, 861, 909, 1290
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 2 cut(s) 621, 895
Tth111I GACNNNGTC 2 cut(s) 160, 1031
VpaK11BI GGWCC 1 cut(s) 1033
XapI RAATTY 1 cut(s) 73
XceI RCATGY 2 cut(s) 228, 758
XcmI CCANNNNNNNNNTGG 2 cut(s) 553, 638
XmiI GTMKAC 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.