FvH4_2g11030

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002385
Physical Location & Seq
Reverse (-)
9664848 .. 9665345
498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11030.t1

Sequence Viewer

Length: 498 bp
ATGTCTTTAACCACAACAGTTCCTTGCAGGTCCCTAACTAGACCTGCCACGTTCAGTCTCTCTCGCACCCTCTGCACAGCCACTGCAGCAAGTGAGACACGAACCAAGAATCTTGAGCGCATTGCTGAGGAGCTCTTGGAGCTCACAAAAATTGAGAGGCACGATTACTCCATCCTCTTCAGGCTCAAATTGGGCCTCAACAGGTATGGCCCTGCAATCTCAGGGGTGGGTCCCTCATCTTCTGAATCAGGACCTGCTTCCACAGACTCTAAGGTGGCAGAGAAGGTCGCATTTGATATAAAGCTTGAGAAGTTTGATGCGGCGGCAAAGATCAAGATTATAAAGGAGGTTAGGACTTTCACTGATTTGGGACTAAAGGATGCTAAAGACTTGGTAGAAAAGGCTCCTGTTGTTCTGAAGAAGGGTGTGACCAAAGAAGAGGCAGGGCCTATTGTTGAGAAGCTTAAGGAGTTGGGTGCTACTGTAGTTTTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

166

Amino Acids

17.92

Weight (kDa)

9.04

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 98 - 164 2.2e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 341
Acc36I ACCTGC 3 cut(s) 18, 52, 262
AciI CCGC 2 cut(s) 320, 323
AcuI CTGAAG 2 cut(s) 163, 437
AflII CTTAAG 1 cut(s) 464
AluBI AGCT 4 cut(s) 133, 142, 304, 463
AluI AGCT 4 cut(s) 133, 142, 304, 463
Alw21I GWGCWC 2 cut(s) 135, 144
Alw26I GTCTC 2 cut(s) 62, 89
AlwNI CAGNNNCTG 2 cut(s) 83, 254
AoxI GGCC 3 cut(s) 193, 208, 446
ApeKI GCWGC 1 cut(s) 86
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 6 cut(s) 30, 193, 209, 230, 251, 446
AvaII GGWCC 3 cut(s) 30, 230, 251
BanII GRGCYC 2 cut(s) 135, 144
Bbv12I GWGCWC 2 cut(s) 135, 144
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 62, 89
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 2 cut(s) 84, 483
BfrI CTTAAG 1 cut(s) 464
BfuAI ACCTGC 3 cut(s) 18, 52, 262
BisI GCNGC 3 cut(s) 87, 321, 324
BlsI GCNGC 3 cut(s) 88, 322, 325
Bme18I GGWCC 3 cut(s) 30, 230, 251
BmgT120I GGNCC 6 cut(s) 30, 193, 209, 230, 251, 446
BmiI GGNNCC 4 cut(s) 32, 231, 232, 405
BmsI GCATC 2 cut(s) 307, 370
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 134, 326
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse3DI GCAATG 1 cut(s) 120
BseGI GGATG 2 cut(s) 171, 385
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 2 cut(s) 117, 234
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 58
BshFI GGCC 3 cut(s) 195, 210, 448
BsiHKAI GWGCWC 2 cut(s) 135, 144
BslFI GGGAC 3 cut(s) 16, 216, 384
BsmAI GTCTC 2 cut(s) 62, 89
BsmFI GGGAC 3 cut(s) 16, 216, 384
BsnI GGCC 3 cut(s) 195, 210, 448
Bsp1286I GDGCHC 2 cut(s) 135, 144
Bsp143I GATC 1 cut(s) 330
BspACI CCGC 2 cut(s) 320, 323
BspANI GGCC 3 cut(s) 195, 210, 448
BspCNI CTCAG 2 cut(s) 118, 233
BspLI GGNNCC 4 cut(s) 32, 231, 232, 405
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 3 cut(s) 18, 52, 262
BspTI CTTAAG 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 120
BssMI GATC 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 19, 484
Bst6I CTCTTC 2 cut(s) 182, 432
BstAFI CTTAAG 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 72
BstDEI CTNAG 3 cut(s) 126, 220, 270
BstF5I GGATG 2 cut(s) 171, 385
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 1 cut(s) 333
BstMAI GTCTC 2 cut(s) 62, 89
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 3 cut(s) 72, 86, 139
BstSFI CTRYAG 2 cut(s) 84, 483
BstV1I GCAGC 1 cut(s) 98
BsuRI GGCC 3 cut(s) 195, 210, 448
BtsCI GGATG 2 cut(s) 171, 385
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 81, 360
BveI ACCTGC 3 cut(s) 18, 52, 262
CaiI CAGNNNCTG 2 cut(s) 83, 254
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 6 cut(s) 30, 193, 209, 230, 251, 446
DdeI CTNAG 3 cut(s) 126, 220, 270
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 182, 432
EarI CTCTTC 2 cut(s) 182, 432
Ecl136II GAGCTC 2 cut(s) 133, 142
Eco24I GRGCYC 2 cut(s) 135, 144
Eco47I GGWCC 3 cut(s) 30, 230, 251
Eco53kI GAGCTC 2 cut(s) 133, 142
Eco57I CTGAAG 2 cut(s) 163, 437
EcoICRI GAGCTC 2 cut(s) 133, 142
EcoO109I RGGNCCY 4 cut(s) 30, 230, 251, 446
EcoT38I GRGCYC 2 cut(s) 135, 144
FaiI YATR 3 cut(s) 207, 299, 341
FaqI GGGAC 3 cut(s) 16, 216, 384
Fnu4HI GCNGC 3 cut(s) 87, 321, 324
FokI GGATG 2 cut(s) 158, 392
FriOI GRGCYC 2 cut(s) 135, 144
Fsp4HI GCNGC 3 cut(s) 87, 321, 324
FspBI CTAG 1 cut(s) 39
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 87, 321, 324
HaeIII GGCC 3 cut(s) 195, 210, 448
HhaI GCGC 1 cut(s) 120
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HindIII AAGCTT 2 cut(s) 302, 461
HinfI GANTC 3 cut(s) 109, 245, 266
Hpy188I TCNGA 2 cut(s) 244, 417
Hpy188III TCNNGA 3 cut(s) 113, 249, 334
HpyAV CCTTC 2 cut(s) 277, 415
HpyCH4III ACNGT 2 cut(s) 19, 484
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 4 cut(s) 27, 75, 86, 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 86, 139
HpyF3I CTNAG 3 cut(s) 126, 220, 270
HpySE526I ACGT 1 cut(s) 50
HspAI GCGC 1 cut(s) 118
KflI GGGWCCC 1 cut(s) 230
Kzo9I GATC 1 cut(s) 330
LmnI GCTCC 3 cut(s) 130, 139, 409
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 2 cut(s) 307, 370
MaeI CTAG 1 cut(s) 39
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 427
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 4 cut(s) 169, 231, 430, 449
MhlI GDGCHC 2 cut(s) 135, 144
MluCI AATT 2 cut(s) 150, 188
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 8 cut(s) 80, 121, 150, 185, 206, 244, 340, 433
MseI TTAA 2 cut(s) 8, 465
MspCI CTTAAG 1 cut(s) 464
MwoI GCNNNNNNNGC 3 cut(s) 72, 86, 139
NdeII GATC 1 cut(s) 330
NlaIV GGNNCC 4 cut(s) 32, 231, 232, 405
NmuCI GTSAC 1 cut(s) 427
PfeI GAWTC 2 cut(s) 109, 245
PkrI GCNGC 3 cut(s) 88, 322, 325
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 3 cut(s) 30, 230, 251
PsiI TTATAA 1 cut(s) 341
Psp124BI GAGCTC 2 cut(s) 135, 144
Psp5II RGGWCCY 3 cut(s) 30, 230, 251
PspN4I GGNNCC 4 cut(s) 32, 231, 232, 405
PspPI GGNCC 6 cut(s) 30, 193, 209, 230, 251, 446
PspPPI RGGWCCY 3 cut(s) 30, 230, 251
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 2 cut(s) 83, 254
SacI GAGCTC 2 cut(s) 135, 144
SaqAI TTAA 2 cut(s) 8, 465
SatI GCNGC 3 cut(s) 87, 321, 324
Sau3AI GATC 1 cut(s) 330
Sau96I GGNCC 6 cut(s) 30, 193, 209, 230, 251, 446
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 135, 144
SfaNI GCATC 2 cut(s) 307, 370
SfcI CTRYAG 2 cut(s) 84, 483
SinI GGWCC 3 cut(s) 30, 230, 251
SmlI CTYRAG 3 cut(s) 113, 305, 464
SmoI CTYRAG 3 cut(s) 113, 305, 464
Sse9I AATT 2 cut(s) 150, 188
SsiI CCGC 2 cut(s) 320, 323
SspMI CTAG 1 cut(s) 39
SstI GAGCTC 2 cut(s) 135, 144
TaaI ACNGT 2 cut(s) 19, 484
TaiI ACGT 1 cut(s) 53
TasI AATT 2 cut(s) 150, 188
TauI GCSGC 2 cut(s) 323, 326
TfiI GAWTC 2 cut(s) 109, 245
Tru1I TTAA 2 cut(s) 8, 465
Tru9I TTAA 2 cut(s) 8, 465
TscAI CASTG 2 cut(s) 88, 367
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 427
TspRI CASTG 2 cut(s) 88, 367
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 3 cut(s) 30, 230, 251
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.