Rh6DG157700

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002385
Physical Location & Seq
Reverse (-)
23582203 .. 23586219
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG157700.1

Sequence Viewer

Length: 948 bp
ATGGCATGCTCTTTACGACGCTTCTCCAGTCATGCTCGTATTTCTCAAACCACTATTACCGCCTTAGGGTCCAAAGACCATCTGCAAAGCAAGGCTATAATACACCCACCTGCCAGAACTGTGGCTCAACAAATATCAGGGTCCCTATCCAGATCCACCACATTCAGTCACTCTCGCACCCTCTGCACAGCCACTGCAGCAAGCGAGACACGGACTAAGAATCTTGAGCGCATTGCTGATGAGCTCTTGGACCTCTCAAAGATTGAGAGGCATGATTACTCCATCCTCTTCAGGCTCAAAATGGGCCTCAACAGGTATGGCCCTGCAATCTCAGGGATGAGTGCCGCATCTTCTGAATCTGGGCCTGCTTCCACAGACTCTAAGGTGGCAGAGAAGGTCGCATTTGATATAAAGCTTGAGAAGTTTGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACAGATTTGGGACTAAAGGACGCTAAAGAGTTGGTGGAAAAGGCTCCTGTTGTGCTGAAGAAGGGTGTGACCAAAGAGGAGGCAGGGCCTATTGTTGACAAGCTTAAGGAATTCGATACCTTGACGTTCGCCAAGATGTTGATCACAGCTTGTAGATGCACTCATCATTGGTACAACGGCTTTCTCACAGAATTGGAGTTTAACAGAATAGTAGATGTCCATGGTGTTCGGAATATGAAAGATCCTCGAGGTATAGAGACAATTCGGTCTGGTGGCTCACTCAAGGAGAAAGAAGTGTGGCGACTGTCAAGGAGGAAAGATGTGATGCACAGTGCGCTAAAAAGGATACTAGTTGGTTTAAAACAACATATCATCGAGTCAAATCTATGTTTTGTAGCTGTGTTAGGGACGATTACAAGATTAAAGTTTGGAACTACAAGATGCATAGATGTCTTTTTACTCTTCTCGAGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

315

Amino Acids

35.08

Weight (kDa)

9.73

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 135 - 194 2.4e-18 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
AasI GACNNNNNNGTC 1 cut(s) 736
AbsI CCTCGAGG 1 cut(s) 717
Acc36I ACCTGC 1 cut(s) 118
AciI CCGC 3 cut(s) 60, 345, 434
AclWI GGATC 2 cut(s) 147, 707
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 274, 548
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 66
AflII CTTAAG 1 cut(s) 575
AhlI ACTAGT 1 cut(s) 820
AluBI AGCT 5 cut(s) 244, 415, 574, 620, 869
AluI AGCT 5 cut(s) 244, 415, 574, 620, 869
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 200, 722
AlwI GGATC 2 cut(s) 147, 707
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 2 cut(s) 717, 937
AoxI GGCC 4 cut(s) 304, 319, 362, 557
ApeKI GCWGC 2 cut(s) 197, 431
ApoI RAATTY 1 cut(s) 581
AspLEI GCGC 2 cut(s) 231, 808
AspS9I GGNCC 7 cut(s) 69, 141, 250, 304, 320, 362, 557
AvaI CYCGRG 2 cut(s) 717, 937
AvaII GGWCC 3 cut(s) 69, 141, 250
AxyI CCTNAGG 1 cut(s) 64
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 209, 443
BccI CCATC 2 cut(s) 87, 290
BceAI ACGGC 1 cut(s) 664
BciVI GTATCC 1 cut(s) 810
BclI TGATCA 1 cut(s) 612
BcoDI GTCTC 2 cut(s) 200, 722
BcuI ACTAGT 1 cut(s) 820
BfaI CTAG 2 cut(s) 821, 946
BfmI CTRYAG 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 118
BfuI GTATCC 1 cut(s) 810
BisI GCNGC 4 cut(s) 198, 345, 432, 435
BlsI GCNGC 4 cut(s) 199, 346, 433, 436
Bme18I GGWCC 3 cut(s) 69, 141, 250
BmeT110I CYCGRG 2 cut(s) 717, 937
BmgT120I GGNCC 7 cut(s) 69, 141, 250, 304, 320, 362, 557
BmiI GGNNCC 4 cut(s) 70, 142, 143, 516
BmsI GCATC 4 cut(s) 356, 617, 786, 902
BpmI CTGGAG 1 cut(s) 10
BpuEI CTTGAG 3 cut(s) 245, 437, 737
BsaBI GATNNNNATC 2 cut(s) 446, 611
BsaJI CCNNGG 1 cut(s) 691
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse1I ACTGG 1 cut(s) 27
Bse21I CCTNAGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 2 cut(s) 446, 611
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 2 cut(s) 282, 342
BseJI GATNNNNATC 2 cut(s) 446, 611
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 2 cut(s) 209, 443
BsgI GTGCAG 1 cut(s) 169
BshFI GGCC 4 cut(s) 306, 321, 364, 559
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 2 cut(s) 717, 937
BslFI GGGAC 3 cut(s) 127, 495, 892
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 2 cut(s) 200, 722
BsmFI GGGAC 3 cut(s) 127, 495, 892
BsnI GGCC 4 cut(s) 306, 321, 364, 559
BsoBI CYCGRG 2 cut(s) 717, 937
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 5 cut(s) 152, 441, 447, 612, 712
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 3 cut(s) 60, 345, 434
BspANI GGCC 4 cut(s) 306, 321, 364, 559
BspCNI CTCAG 1 cut(s) 344
BspLI GGNNCC 4 cut(s) 70, 142, 143, 516
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 2 cut(s) 147, 707
BspTI CTTAAG 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 231
BsrI ACTGG 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 5 cut(s) 152, 441, 447, 612, 712
BssT1I CCWWGG 1 cut(s) 691
Bst4CI ACNGT 3 cut(s) 121, 777, 803
Bst6I CTCTTC 2 cut(s) 293, 938
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 7, 202, 366
BstDEI CTNAG 4 cut(s) 64, 216, 331, 381
BstDSI CCRYGG 1 cut(s) 691
BstF5I GGATG 2 cut(s) 282, 342
BstHHI GCGC 2 cut(s) 231, 808
BstKTI GATC 5 cut(s) 155, 444, 450, 615, 715
BstMAI GTCTC 2 cut(s) 200, 722
BstMBI GATC 5 cut(s) 152, 441, 447, 612, 712
BstMWI GCNNNNNNNGC 3 cut(s) 183, 197, 805
BstNSI RCATGY 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 209, 443
BstX2I RGATCY 2 cut(s) 152, 712
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 2 cut(s) 152, 712
Bsu36I CCTNAGG 1 cut(s) 64
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 4 cut(s) 306, 321, 364, 559
BtgI CCRYGG 1 cut(s) 691
BtsCI GGATG 2 cut(s) 282, 342
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 192, 808
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 3 cut(s) 7, 202, 366
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 2 cut(s) 231, 808
Cfr13I GGNCC 7 cut(s) 69, 141, 250, 304, 320, 362, 557
CseI GACGC 3 cut(s) 27, 437, 500
Csp6I GTAC 1 cut(s) 643
CviAII CATG 4 cut(s) 6, 32, 272, 692
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 4 cut(s) 64, 216, 331, 381
DpnI GATC 5 cut(s) 154, 443, 449, 614, 714
DpnII GATC 5 cut(s) 152, 441, 447, 612, 712
DraI TTTAAA 1 cut(s) 831
DrdI GACNNNNNNGTC 1 cut(s) 736
DseDI GACNNNNNNGTC 1 cut(s) 736
Eam1104I CTCTTC 2 cut(s) 293, 938
EarI CTCTTC 2 cut(s) 293, 938
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 3 cut(s) 69, 141, 250
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 2 cut(s) 274, 548
Eco81I CCTNAGG 1 cut(s) 64
Eco88I CYCGRG 2 cut(s) 717, 937
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 2 cut(s) 141, 557
EcoRI GAATTC 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 917
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 691
FaeI CATG 4 cut(s) 9, 35, 275, 695
FaqI GGGAC 3 cut(s) 127, 495, 892
FatI CATG 4 cut(s) 5, 31, 271, 691
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 4 cut(s) 198, 345, 432, 435
FokI GGATG 2 cut(s) 269, 349
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 4 cut(s) 198, 345, 432, 435
FspBI CTAG 2 cut(s) 821, 946
GlaI GCGC 2 cut(s) 230, 807
GluI GCNGC 4 cut(s) 198, 345, 432, 435
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 4 cut(s) 306, 321, 364, 559
HgaI GACGC 3 cut(s) 27, 437, 500
HhaI GCGC 2 cut(s) 231, 808
Hin1II CATG 4 cut(s) 9, 35, 275, 695
Hin6I GCGC 2 cut(s) 229, 806
HinP1I GCGC 2 cut(s) 229, 806
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 413, 572
HinfI GANTC 4 cut(s) 220, 356, 377, 848
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 2 cut(s) 355, 702
Hpy188III TCNNGA 4 cut(s) 150, 224, 445, 937
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 2 cut(s) 388, 526
HpyCH4III ACNGT 3 cut(s) 121, 777, 803
HpyCH4IV ACGT 1 cut(s) 596
HpyCH4V TGCA 7 cut(s) 85, 186, 197, 326, 630, 799, 915
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 197, 805
HpyF3I CTNAG 4 cut(s) 64, 216, 331, 381
HpySE526I ACGT 1 cut(s) 596
Hsp92II CATG 4 cut(s) 9, 35, 275, 695
HspAI GCGC 2 cut(s) 229, 806
KflI GGGWCCC 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 5 cut(s) 152, 441, 447, 612, 712
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 2 cut(s) 209, 443
LweI GCATC 4 cut(s) 356, 617, 786, 902
MaeI CTAG 2 cut(s) 821, 946
MaeII ACGT 1 cut(s) 596
MaeIII GTNAC 2 cut(s) 167, 538
MalI GATC 5 cut(s) 154, 443, 449, 614, 714
MboI GATC 5 cut(s) 152, 441, 447, 612, 712
MboII GAAGA 4 cut(s) 280, 342, 541, 925
MflI RGATCY 2 cut(s) 152, 712
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 3 cut(s) 581, 662, 732
MlyI GAGTC 2 cut(s) 371, 857
Mph1103I ATGCAT 1 cut(s) 917
MseI TTAA 4 cut(s) 576, 672, 830, 893
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 183, 197, 805
NcoI CCATGG 1 cut(s) 691
NdeII GATC 5 cut(s) 152, 441, 447, 612, 712
NlaIII CATG 4 cut(s) 9, 35, 275, 695
NlaIV GGNNCC 4 cut(s) 70, 142, 143, 516
NmuCI GTSAC 2 cut(s) 167, 538
NsiI ATGCAT 1 cut(s) 917
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PaeR7I CTCGAG 2 cut(s) 717, 937
PaqCI CACCTGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 220, 356
PkrI GCNGC 4 cut(s) 199, 346, 433, 436
PleI GAGTC 2 cut(s) 371, 856
PpsI GAGTC 2 cut(s) 371, 856
PpuMI RGGWCCY 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 70, 142, 143, 516
PspPI GGNCC 7 cut(s) 69, 141, 250, 304, 320, 362, 557
PspPPI RGGWCCY 1 cut(s) 141
PspXI VCTCGAGB 1 cut(s) 717
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 2 cut(s) 152, 712
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 4 cut(s) 576, 672, 830, 893
SatI GCNGC 4 cut(s) 198, 345, 432, 435
Sau3AI GATC 5 cut(s) 152, 441, 447, 612, 712
Sau96I GGNCC 7 cut(s) 69, 141, 250, 304, 320, 362, 557
SchI GAGTC 2 cut(s) 371, 857
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 4 cut(s) 356, 617, 786, 902
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 2 cut(s) 717, 937
SinI GGWCC 3 cut(s) 69, 141, 250
SlaI CTCGAG 2 cut(s) 717, 937
SmlI CTYRAG 6 cut(s) 224, 416, 575, 717, 752, 937
SmoI CTYRAG 6 cut(s) 224, 416, 575, 717, 752, 937
SpeI ACTAGT 1 cut(s) 820
SphI GCATGC 1 cut(s) 9
Sse9I AATT 3 cut(s) 581, 662, 732
SsiI CCGC 3 cut(s) 60, 345, 434
SspMI CTAG 2 cut(s) 821, 946
SstI GAGCTC 1 cut(s) 246
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 3 cut(s) 121, 777, 803
TaiI ACGT 1 cut(s) 599
TaqI TCGA 4 cut(s) 585, 718, 846, 938
TaqII GACCGA 1 cut(s) 726
TasI AATT 3 cut(s) 581, 662, 732
TauI GCSGC 2 cut(s) 347, 437
TfiI GAWTC 2 cut(s) 220, 356
Tru1I TTAA 4 cut(s) 576, 672, 830, 893
Tru9I TTAA 4 cut(s) 576, 672, 830, 893
TscAI CASTG 2 cut(s) 199, 808
TseFI GTSAC 2 cut(s) 167, 538
TseI GCWGC 2 cut(s) 197, 431
Tsp45I GTSAC 2 cut(s) 167, 538
TspDTI ATGAA 1 cut(s) 722
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 2 cut(s) 199, 808
Vha464I CTTAAG 1 cut(s) 575
VpaK11BI GGWCC 3 cut(s) 69, 141, 250
XapI RAATTY 1 cut(s) 581
XceI RCATGY 1 cut(s) 9
XhoI CTCGAG 2 cut(s) 717, 937
XspI CTAG 2 cut(s) 821, 946
Zsp2I ATGCAT 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.