Prupe.1G195400_v2.0.a1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002385
Physical Location & Seq
Forward (+)
18514600 .. 18516953
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G195400.1

Sequence Viewer

Length: 648 bp
ATGGCTAGCTCTATACAACGCTTCTCCAGCCGTGCTTGTGTTTCTCGAACCACTATTGTCCAAGGGTCAAAACCATTCCTGGTGGACGCGGTCGGGATTGTCCCTAGCATTCCAGAAGAAAATTGTCACAGTGAGGTTGTAATAGGCTCACCTGCTAGGACTGCAACTCAACAAGGATCAGGGTCCGTATCAAGATTTGTTACTGCTCTTTCTCGCCCTTTCAGCACAGCCACTACAGCAAATGAGACTCAGACTCAGAAGCTTGAGCGCATCGCTGACGAGCTCCTCAACCTCACAAAGATCGAGAGGCATGATTACTCCATCCTCTTCAGGCTAAAAATGGGCCTCAATCGGTATGGCCCGGCAATCTCTGGAATTGGCCCGACATCTTCTGAATCTGGGTCTGCCTCCACAGATGCCAAGGCCGTTGAGAAGACTGCATTTGATATAAAGATTGAGAAGTTTGATGCAGCGGCAAAGATCAAGATCATTAAGGAGGTTAGGACTTTCACTGACTTAGGACTGAAGGAGGCTAAGGAGTTGGTAGAGAAGGTCCCTGTTGTGGTGAAAAAGGGCCTGACTAAAGAGGAGGCAGGGCCCATTGTTGACAAGCTTAAGGAGTTGGGCGCTACTGTGGTATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

216

Amino Acids

23.14

Weight (kDa)

9.02

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccII CGCG 1 cut(s) 89
AciI CCGC 2 cut(s) 89, 473
AclWI GGATC 1 cut(s) 184
AcuI CTGAAG 2 cut(s) 313, 545
AfiI CCNNNNNNNGG 1 cut(s) 562
AflII CTTAAG 1 cut(s) 614
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 4 cut(s) 9, 262, 283, 613
AluI AGCT 4 cut(s) 9, 262, 283, 613
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 184
AoxI GGCC 6 cut(s) 343, 358, 379, 423, 574, 596
ApaI GGGCCC 1 cut(s) 600
ApeKI GCWGC 1 cut(s) 470
AspLEI GCGC 2 cut(s) 270, 629
AspS9I GGNCC 8 cut(s) 183, 343, 359, 380, 553, 574, 596, 597
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 141, 577
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 183, 553
BaeGI GKGCMC 1 cut(s) 600
BanII GRGCYC 2 cut(s) 285, 600
BbsI GAAGAC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 482
BccI CCATC 1 cut(s) 329
BceAI ACGGC 2 cut(s) 15, 410
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 3 cut(s) 6, 105, 156
BfmI CTRYAG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 630
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 2 cut(s) 471, 474
BlsI GCNGC 2 cut(s) 472, 475
Bme1390I CCNGG 2 cut(s) 80, 362
Bme18I GGWCC 2 cut(s) 183, 553
BmgT120I GGNCC 8 cut(s) 183, 343, 359, 380, 553, 574, 596, 597
BmiI GGNNCC 3 cut(s) 184, 555, 598
BmrFI CCNGG 2 cut(s) 80, 362
BmsI GCATC 3 cut(s) 279, 406, 457
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 362
BsaBI GATNNNNATC 1 cut(s) 485
BsaJI CCNNGG 2 cut(s) 61, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 61, 420
BseGI GGATG 1 cut(s) 321
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 1 cut(s) 562
BseMII CTCAG 2 cut(s) 263, 269
BseRI GAGGAG 2 cut(s) 275, 602
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 1 cut(s) 482
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 345, 360, 381, 425, 576, 598
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 2 cut(s) 86, 539
BslI CCNNNNNNNGG 1 cut(s) 562
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 2 cut(s) 86, 539
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 6 cut(s) 345, 360, 381, 425, 576, 598
Bsp120I GGGCCC 1 cut(s) 596
Bsp1286I GDGCHC 2 cut(s) 285, 600
Bsp143I GATC 4 cut(s) 176, 300, 480, 486
BspACI CCGC 2 cut(s) 89, 473
BspANI GGCC 6 cut(s) 345, 360, 381, 425, 576, 598
BspCNI CTCAG 2 cut(s) 262, 268
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 3 cut(s) 184, 555, 598
BspMI ACCTGC 1 cut(s) 160
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 184
BspTI CTTAAG 1 cut(s) 614
BssECI CCNNGG 2 cut(s) 61, 420
BssMI GATC 4 cut(s) 176, 300, 480, 486
BssT1I CCWWGG 2 cut(s) 61, 420
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 131, 634
Bst6I CTCTTC 1 cut(s) 332
BstAFI CTTAAG 1 cut(s) 614
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 249, 255, 517, 534
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 2 cut(s) 270, 629
BstKTI GATC 4 cut(s) 179, 303, 483, 489
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 4 cut(s) 176, 300, 480, 486
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 4 cut(s) 27, 161, 222, 236
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 360
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 600
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 440
BsuRI GGCC 6 cut(s) 345, 360, 381, 425, 576, 598
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 136, 510
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 270, 629
Cfr13I GGNCC 8 cut(s) 183, 343, 359, 380, 553, 574, 596, 597
CseI GACGC 1 cut(s) 95
CviAII CATG 1 cut(s) 311
DdeI CTNAG 4 cut(s) 249, 255, 517, 534
DpnI GATC 4 cut(s) 178, 302, 482, 488
DpnII GATC 4 cut(s) 176, 300, 480, 486
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 61, 420
Eco24I GRGCYC 2 cut(s) 285, 600
Eco47I GGWCC 2 cut(s) 183, 553
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 313, 545
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 3 cut(s) 553, 574, 596
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 61, 420
EcoT38I GRGCYC 2 cut(s) 285, 600
ErhI CCWWGG 2 cut(s) 61, 420
FaeI CATG 1 cut(s) 314
FaiI YATR 4 cut(s) 14, 312, 357, 449
FaqI GGGAC 2 cut(s) 86, 539
FatI CATG 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 471, 474
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 2 cut(s) 285, 600
Fsp4HI GCNGC 2 cut(s) 471, 474
FspBI CTAG 3 cut(s) 6, 105, 156
GlaI GCGC 2 cut(s) 269, 628
GluI GCNGC 2 cut(s) 471, 474
GsuI CTGGAG 1 cut(s) 10
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 6 cut(s) 345, 360, 381, 425, 576, 598
HapII CCGG 1 cut(s) 362
HgaI GACGC 1 cut(s) 95
HhaI GCGC 2 cut(s) 270, 629
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 2 cut(s) 268, 627
HinP1I GCGC 2 cut(s) 268, 627
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 2 cut(s) 260, 611
HinfI GANTC 3 cut(s) 247, 253, 395
HpaII CCGG 1 cut(s) 362
HphI GGTGA 2 cut(s) 141, 577
Hpy166II GTNNAC 2 cut(s) 85, 607
Hpy188I TCNGA 3 cut(s) 252, 258, 394
Hpy188III TCNNGA 7 cut(s) 45, 94, 113, 192, 304, 372, 484
Hpy8I GTNNAC 2 cut(s) 85, 607
HpyAV CCTTC 2 cut(s) 520, 544
HpyCH4III ACNGT 2 cut(s) 131, 634
HpyCH4V TGCA 3 cut(s) 164, 440, 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 161, 222, 236
HpyF3I CTNAG 4 cut(s) 249, 255, 517, 534
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 2 cut(s) 268, 627
Kzo9I GATC 4 cut(s) 176, 300, 480, 486
LmnI GCTCC 1 cut(s) 288
Lsp1109I GCAGC 1 cut(s) 482
LweI GCATC 3 cut(s) 279, 406, 457
MaeI CTAG 3 cut(s) 6, 105, 156
MaeIII GTNAC 2 cut(s) 125, 199
MalI GATC 4 cut(s) 178, 302, 482, 488
MboI GATC 4 cut(s) 176, 300, 480, 486
MboII GAAGA 4 cut(s) 128, 319, 381, 445
MhlI GDGCHC 2 cut(s) 285, 600
MluCI AATT 2 cut(s) 121, 375
MlyI GAGTC 2 cut(s) 241, 247
MseI TTAA 2 cut(s) 492, 615
MspA1I CMGCKG 1 cut(s) 473
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 2 cut(s) 80, 362
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 4 cut(s) 27, 161, 222, 236
NciI CCSGG 1 cut(s) 362
NdeII GATC 4 cut(s) 176, 300, 480, 486
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 3 cut(s) 184, 555, 598
NmuCI GTSAC 1 cut(s) 125
PaqCI CACCTGC 1 cut(s) 160
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 395
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 472, 475
PleI GAGTC 2 cut(s) 241, 247
PpsI GAGTC 2 cut(s) 241, 247
PpuMI RGGWCCY 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 3 cut(s) 184, 555, 598
PspOMI GGGCCC 1 cut(s) 596
PspPI GGNCC 8 cut(s) 183, 343, 359, 380, 553, 574, 596, 597
PspPPI RGGWCCY 1 cut(s) 553
PsyI GACNNNGTC 1 cut(s) 89
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 492, 615
SatI GCNGC 2 cut(s) 471, 474
Sau3AI GATC 4 cut(s) 176, 300, 480, 486
Sau96I GGNCC 8 cut(s) 183, 343, 359, 380, 553, 574, 596, 597
SchI GAGTC 2 cut(s) 241, 247
ScrFI CCNGG 2 cut(s) 80, 362
SduI GDGCHC 2 cut(s) 285, 600
SetI ASST 9 cut(s) 11, 138, 154, 264, 285, 294, 501, 555, 615
SfaNI GCATC 3 cut(s) 279, 406, 457
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 2 cut(s) 183, 553
SmlI CTYRAG 2 cut(s) 263, 614
SmoI CTYRAG 2 cut(s) 263, 614
Sse9I AATT 2 cut(s) 121, 375
SsiI CCGC 2 cut(s) 89, 473
SspMI CTAG 3 cut(s) 6, 105, 156
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 78, 360
StyI CCWWGG 2 cut(s) 61, 420
TaaI ACNGT 2 cut(s) 131, 634
TaqI TCGA 2 cut(s) 46, 303
TasI AATT 2 cut(s) 121, 375
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 492, 615
Tru9I TTAA 2 cut(s) 492, 615
TscAI CASTG 2 cut(s) 136, 517
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 470
Tsp45I GTSAC 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 136, 517
Tth111I GACNNNGTC 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 183, 553
XspI CTAG 3 cut(s) 6, 105, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.