Rw7G025980

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002385
Physical Location & Seq
Forward (+)
32858901 .. 32859563
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025980.1

Sequence Viewer

Length: 663 bp
ATGAACGTCAATATACGTATACCGTTGCTATACTTGAGCCTCTGTCATGATGTTTCGACATGGAAACTGAACTACCTGACTTTCAGGAAATGTAATGATTGTCTTGTAGTCCAGCTAGGTAGTGAATTTCTGTCGCACAAAACCCCAACCCTTTTATCTTTCCAGAACGCCCTTAACCACAAATGTTCCCTGCATGTCCCAATCCGGACCTGCCTCGAGCAGCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCGTCGGTCCAAGAATCTTGAGCGCATTACTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAAACTTAAAATGGGCCTCAACAGATATGGTCCAGCTGTCTCAGGGATAGGCCCGACATCTTCCGAATCTGGGTCTGCCTCCACAGGCTCCAAGGTGGTGGTGAAGACCGCATTTGATATAAAGCTTGAGAAGTTTGACGTAGTGGCAAAGATCAAAATCATAAAGGAGGTTAGGACTTTCATTGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAAATGGGTGTCACCAGAGAGGAGGCAGGCCCCATTGTTGACAGGCTCAAGGAGTTGGGTGCTACTGTGGTATTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

220

Amino Acids

24.57

Weight (kDa)

9.41

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 153 - 220 3.4e-19 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 204
AciI CCGC 1 cut(s) 453
AcsI RAATTY 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 2 cut(s) 346, 658
AluBI AGCT 4 cut(s) 115, 223, 380, 469
AluI AGCT 4 cut(s) 115, 223, 380, 469
Alw26I GTCTC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 3 cut(s) 358, 394, 610
ApeKI GCWGC 2 cut(s) 220, 251
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 7 cut(s) 207, 267, 304, 358, 374, 395, 611
AsuHPI GGTGA 2 cut(s) 457, 586
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 4 cut(s) 207, 267, 304, 374
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 2 cut(s) 232, 263
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 218
BisI GCNGC 2 cut(s) 221, 252
BlsI GCNGC 2 cut(s) 222, 253
Bme18I GGWCC 4 cut(s) 207, 267, 304, 374
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 7 cut(s) 207, 267, 304, 358, 374, 395, 611
BmiI GGNNCC 3 cut(s) 433, 570, 613
BpiI GAAGAC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 4 cut(s) 55, 299, 491, 614
BsaAI YACGTR 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 1 cut(s) 572
Bse8I GATNNNNATC 1 cut(s) 500
BseAI TCCGGA 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 572
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 2 cut(s) 232, 263
BshFI GGCC 3 cut(s) 360, 396, 612
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 2 cut(s) 182, 549
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 2 cut(s) 182, 549
BsnI GGCC 3 cut(s) 360, 396, 612
BsoBI CYCGRG 1 cut(s) 215
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 495
BspACI CCGC 1 cut(s) 453
BspANI GGCC 3 cut(s) 360, 396, 612
BspCNI CTCAG 1 cut(s) 398
BspEI TCCGGA 1 cut(s) 204
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 433, 570, 613
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 218
BsrI ACTGG 1 cut(s) 572
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 1 cut(s) 495
BssNAI GTATAC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 20
Bst4CI ACNGT 2 cut(s) 24, 649
Bst6I CTCTTC 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 1 cut(s) 385
BstF5I GGATG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 1 cut(s) 498
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 1 cut(s) 495
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 249
BstSNI TACGTA 1 cut(s) 17
BstV1I GCAGC 2 cut(s) 232, 263
BstV2I GAAGAC 1 cut(s) 455
BstXI CCANNNNNNTGG 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 20
BsuRI GGCC 3 cut(s) 360, 396, 612
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 610
CaiI CAGNNNCTG 1 cut(s) 248
CciI TCATGA 1 cut(s) 46
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 7 cut(s) 207, 267, 304, 358, 374, 395, 611
CseI GACGC 2 cut(s) 251, 554
CviAII CATG 4 cut(s) 47, 60, 194, 326
DdeI CTNAG 1 cut(s) 385
DpnI GATC 1 cut(s) 497
DpnII GATC 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco105I TACGTA 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 435
Eco47I GGWCC 4 cut(s) 207, 267, 304, 374
Eco88I CYCGRG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 4 cut(s) 50, 63, 197, 329
FaqI GGGAC 2 cut(s) 182, 549
FatI CATG 4 cut(s) 46, 59, 193, 325
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 221, 252
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 221, 252
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 284
GluI GCNGC 2 cut(s) 221, 252
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 3 cut(s) 360, 396, 612
HapII CCGG 1 cut(s) 205
HgaI GACGC 2 cut(s) 251, 554
HhaI GCGC 1 cut(s) 285
Hin1II CATG 4 cut(s) 50, 63, 197, 329
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 2 cut(s) 274, 410
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 457, 586
Hpy166II GTNNAC 2 cut(s) 20, 622
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 5 cut(s) 47, 85, 163, 205, 278
Hpy8I GTNNAC 2 cut(s) 20, 622
Hpy99I CGWCG 1 cut(s) 267
HpyCH4III ACNGT 2 cut(s) 24, 649
HpyCH4IV ACGT 3 cut(s) 6, 16, 483
HpyCH4V TGCA 2 cut(s) 193, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 3 cut(s) 6, 16, 483
Hsp92II CATG 4 cut(s) 50, 63, 197, 329
HspAI GCGC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 204
Kzo9I GATC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 437, 574
Lsp1109I GCAGC 2 cut(s) 232, 263
MaeI CTAG 1 cut(s) 116
MaeII ACGT 3 cut(s) 6, 16, 483
MaeIII GTNAC 1 cut(s) 592
MalI GATC 1 cut(s) 497
MboI GATC 1 cut(s) 495
MboII GAAGA 3 cut(s) 334, 396, 460
MluCI AATT 1 cut(s) 125
MroI TCCGGA 1 cut(s) 204
MseI TTAA 2 cut(s) 174, 351
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 251
NdeII GATC 1 cut(s) 495
NlaIII CATG 4 cut(s) 50, 63, 197, 329
NlaIV GGNNCC 3 cut(s) 433, 570, 613
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 1 cut(s) 197
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 46
PfeI GAWTC 2 cut(s) 274, 410
PkrI GCNGC 2 cut(s) 222, 253
Ppu21I YACGTR 1 cut(s) 17
PspN4I GGNNCC 3 cut(s) 433, 570, 613
PspPI GGNCC 7 cut(s) 207, 267, 304, 358, 374, 395, 611
PspXI VCTCGAGB 1 cut(s) 215
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 380
SaqAI TTAA 2 cut(s) 174, 351
SatI GCNGC 2 cut(s) 221, 252
Sau3AI GATC 1 cut(s) 495
Sau96I GGNCC 7 cut(s) 207, 267, 304, 358, 374, 395, 611
SfcI CTRYAG 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 215
SinI GGWCC 4 cut(s) 207, 267, 304, 374
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 5 cut(s) 34, 215, 278, 470, 629
SmoI CTYRAG 5 cut(s) 34, 215, 278, 470, 629
SnaBI TACGTA 1 cut(s) 17
Sse9I AATT 1 cut(s) 125
SsiI CCGC 1 cut(s) 453
SspMI CTAG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 24, 649
TaiI ACGT 3 cut(s) 9, 19, 486
TaqI TCGA 2 cut(s) 56, 216
TaqII GACCGA 1 cut(s) 255
TasI AATT 1 cut(s) 125
TfiI GAWTC 2 cut(s) 274, 410
Tru1I TTAA 2 cut(s) 174, 351
Tru9I TTAA 2 cut(s) 174, 351
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 2 cut(s) 220, 251
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 2 cut(s) 17, 514
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 4 cut(s) 207, 267, 304, 374
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.