MD04G1041700.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002385
Physical Location & Seq
Forward (+)
4947621 .. 4954834
7214 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1041700.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGCGAGCTCAGTACAACGCTTCTCCACCTATGCTAGGGCTGTAAAATGCTCAGCTCCCAGAACTGCAACTCAACAAGGATCTGGGCCCCTATCCACATTGCCTGCTACTGTTTCTCGCAAGCTCACCACAGCCACTGCAGCAAATGAGACTCAAACACATAAACTTGAGCGCATTGCCGGCGAGCTCCTCGACCTCACAAAGCTTGAGAGGCACGACTACTCCATTCTTTTTAGGCTGAAAATGGGCCTCAACCGGTATGGCCCAGCAGTCTCAGGGATTGGCTCCACATCTTCCGAATCTGGACCGGCCTCCACGGCTGCCAAGTCTGAGAAGACTGCATTTGATATAAAGATAGAGAAGTTCGATGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACGGACTTGGGGCTGAAGGAGGCGAAGGAGTTGGTGGAGAAGGTCCCTGTTGTGGTGAAGAAGGGCATGACGAAAGAGGAGGCAGGGCCCATTGTTGACAAGCTTAAGGAATTGGGTGCTACTGTGGTACTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

183

Amino Acids

19.57

Weight (kDa)

9.57

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 115 - 181 5.7e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 374
AclWI GGATC 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 2 cut(s) 15, 540
AfiI CCNNNNNNNGG 2 cut(s) 36, 463
AflII CTTAAG 1 cut(s) 515
AgeI ACCGGT 1 cut(s) 255
AluBI AGCT 6 cut(s) 9, 56, 124, 187, 205, 514
AluI AGCT 6 cut(s) 9, 56, 124, 187, 205, 514
Alw21I GWGCWC 2 cut(s) 11, 189
Alw26I GTCTC 2 cut(s) 143, 277
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 5 cut(s) 86, 247, 262, 309, 497
ApaI GGGCCC 2 cut(s) 90, 501
ApeKI GCWGC 3 cut(s) 140, 320, 371
AsiGI ACCGGT 1 cut(s) 255
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 8 cut(s) 86, 87, 247, 263, 305, 454, 497, 498
AsuHPI GGTGA 2 cut(s) 118, 478
AvaII GGWCC 2 cut(s) 305, 454
BaeGI GKGCMC 2 cut(s) 90, 501
BanII GRGCYC 4 cut(s) 11, 90, 189, 501
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 11, 189
BbvI GCAGC 3 cut(s) 152, 307, 383
BceAI ACGGC 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 143, 277
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 138
BfrI CTTAAG 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 4 cut(s) 141, 321, 372, 375
BlpI GCTNAGC 1 cut(s) 52
BlsI GCNGC 4 cut(s) 142, 322, 373, 376
Bme18I GGWCC 2 cut(s) 305, 454
BmgT120I GGNCC 8 cut(s) 86, 87, 247, 263, 305, 454, 497, 498
BmiI GGNNCC 5 cut(s) 88, 89, 286, 456, 499
BmsI GCATC 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 341
Bpu1102I GCTNAGC 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 188, 227
BsaBI GATNNNNATC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 463
Bse118I RCCGGY 3 cut(s) 179, 255, 307
Bse1I ACTGG 1 cut(s) 546
Bse3DI GCAATG 2 cut(s) 98, 174
Bse8I GATNNNNATC 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 2 cut(s) 36, 463
BseMI GCAATG 2 cut(s) 98, 174
BseMII CTCAG 4 cut(s) 24, 66, 288, 321
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 2 cut(s) 179, 503
BseSI GKGCMC 2 cut(s) 90, 501
BseXI GCAGC 3 cut(s) 152, 307, 383
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 5 cut(s) 88, 249, 264, 311, 499
BshTI ACCGGT 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 11, 189
BsiSI CCGG 3 cut(s) 180, 256, 308
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 36, 463
BsmAI GTCTC 2 cut(s) 143, 277
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 5 cut(s) 88, 249, 264, 311, 499
Bsp120I GGGCCC 2 cut(s) 86, 497
Bsp1286I GDGCHC 4 cut(s) 11, 90, 189, 501
Bsp143I GATC 3 cut(s) 80, 381, 387
Bsp1720I GCTNAGC 1 cut(s) 52
BspACI CCGC 1 cut(s) 374
BspANI GGCC 5 cut(s) 88, 249, 264, 311, 499
BspCNI CTCAG 4 cut(s) 23, 65, 287, 322
BspLI GGNNCC 5 cut(s) 88, 89, 286, 456, 499
BspMAI CTGCAG 1 cut(s) 142
BspPI GGATC 1 cut(s) 88
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 2 cut(s) 98, 174
BsrFI RCCGGY 3 cut(s) 179, 255, 307
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 3 cut(s) 179, 255, 307
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 80, 381, 387
Bst4CI ACNGT 2 cut(s) 112, 535
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 5 cut(s) 7, 105, 122, 181, 185
BstDEI CTNAG 4 cut(s) 10, 52, 274, 330
BstDSI CCRYGG 1 cut(s) 315
BstENI CCTNNNNNAGG 1 cut(s) 34
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 83, 384, 390
BstMAI GTCTC 2 cut(s) 143, 277
BstMBI GATC 3 cut(s) 80, 381, 387
BstMWI GCNNNNNNNGC 4 cut(s) 140, 180, 211, 317
BstSFI CTRYAG 1 cut(s) 138
BstSLI GKGCMC 2 cut(s) 90, 501
BstV1I GCAGC 3 cut(s) 152, 307, 383
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 5 cut(s) 88, 249, 264, 311, 499
BtgI CCRYGG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 5 cut(s) 7, 105, 122, 181, 185
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 3 cut(s) 179, 255, 307
Cfr13I GGNCC 8 cut(s) 86, 87, 247, 263, 305, 454, 497, 498
Csp6I GTAC 2 cut(s) 14, 539
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 1 cut(s) 478
CviQI GTAC 2 cut(s) 14, 539
DdeI CTNAG 4 cut(s) 10, 52, 274, 330
DpnI GATC 3 cut(s) 82, 383, 389
DpnII GATC 3 cut(s) 80, 381, 387
Ecl136II GAGCTC 2 cut(s) 9, 187
Eco24I GRGCYC 4 cut(s) 11, 90, 189, 501
Eco47I GGWCC 2 cut(s) 305, 454
Eco53kI GAGCTC 2 cut(s) 9, 187
Eco57I CTGAAG 1 cut(s) 446
EcoICRI GAGCTC 2 cut(s) 9, 187
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoO109I RGGNCCY 3 cut(s) 87, 454, 497
EcoT38I GRGCYC 4 cut(s) 11, 90, 189, 501
FaeI CATG 1 cut(s) 481
FaiI YATR 6 cut(s) 33, 162, 261, 350, 392, 479
FaqI GGGAC 1 cut(s) 440
FatI CATG 1 cut(s) 477
Fnu4HI GCNGC 4 cut(s) 141, 321, 372, 375
FriOI GRGCYC 4 cut(s) 11, 90, 189, 501
Fsp4HI GCNGC 4 cut(s) 141, 321, 372, 375
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 173
GluI GCNGC 4 cut(s) 141, 321, 372, 375
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 5 cut(s) 88, 249, 264, 311, 499
HapII CCGG 3 cut(s) 180, 256, 308
HhaI GCGC 1 cut(s) 174
Hin1II CATG 1 cut(s) 481
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 203, 512
HinfI GANTC 2 cut(s) 151, 299
HpaII CCGG 3 cut(s) 180, 256, 308
HphI GGTGA 2 cut(s) 118, 478
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 2 cut(s) 298, 331
Hpy188III TCNNGA 2 cut(s) 303, 385
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 4 cut(s) 421, 430, 445, 466
HpyCH4III ACNGT 2 cut(s) 112, 535
HpyCH4V TGCA 4 cut(s) 68, 140, 341, 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 180, 211, 317
HpyF3I CTNAG 4 cut(s) 10, 52, 274, 330
Hsp92II CATG 1 cut(s) 481
HspAI GCGC 1 cut(s) 172
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 3 cut(s) 80, 381, 387
LmnI GCTCC 3 cut(s) 61, 192, 290
Lsp1109I GCAGC 3 cut(s) 152, 307, 383
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 36
MalI GATC 3 cut(s) 82, 383, 389
MboI GATC 3 cut(s) 80, 381, 387
MboII GAAGA 3 cut(s) 285, 346, 481
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 4 cut(s) 11, 90, 189, 501
MluCI AATT 1 cut(s) 521
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 9 cut(s) 200, 204, 206, 260, 322, 391, 424, 481, 484
MroNI GCCGGC 1 cut(s) 179
MseI TTAA 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 3 cut(s) 180, 256, 308
MwoI GCNNNNNNNGC 4 cut(s) 140, 180, 211, 317
NaeI GCCGGC 1 cut(s) 181
NdeII GATC 3 cut(s) 80, 381, 387
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 5 cut(s) 88, 89, 286, 456, 499
PdiI GCCGGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 299
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 4 cut(s) 142, 322, 373, 376
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 454
Psp124BI GAGCTC 2 cut(s) 11, 189
Psp5II RGGWCCY 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 88, 89, 286, 456, 499
PspOMI GGGCCC 2 cut(s) 86, 497
PspPI GGNCC 8 cut(s) 86, 87, 247, 263, 305, 454, 497, 498
PspPPI RGGWCCY 1 cut(s) 454
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 80
RsaI GTAC 2 cut(s) 15, 540
RsaNI GTAC 2 cut(s) 14, 539
SacI GAGCTC 2 cut(s) 11, 189
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 4 cut(s) 141, 321, 372, 375
Sau3AI GATC 3 cut(s) 80, 381, 387
Sau96I GGNCC 8 cut(s) 86, 87, 247, 263, 305, 454, 497, 498
SchI GAGTC 1 cut(s) 145
SduI GDGCHC 4 cut(s) 11, 90, 189, 501
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 138
SinI GGWCC 2 cut(s) 305, 454
SmlI CTYRAG 3 cut(s) 167, 206, 515
SmoI CTYRAG 3 cut(s) 167, 206, 515
Sse9I AATT 1 cut(s) 521
SsiI CCGC 1 cut(s) 374
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 2 cut(s) 11, 189
TaaI ACNGT 2 cut(s) 112, 535
TaqI TCGA 2 cut(s) 192, 366
TasI AATT 1 cut(s) 521
TatI WGTACW 1 cut(s) 13
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 1 cut(s) 142
TseI GCWGC 3 cut(s) 140, 320, 371
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 1 cut(s) 142
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 305, 454
XagI CCTNNNNNAGG 1 cut(s) 34
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.