RLG00000013869

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002385
Physical Location & Seq
Forward (+)
43324055 .. 43325568
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013869

Sequence Viewer

Length: 609 bp
ATGGCATGCTCTTTACGACGCTTCTCGAGTCATGCTCGTATTTCTCAAACCACTATTACCACCTTAGGGTCCAAAGACCATCTTCAAAGCAAGGCTATAATACACTCACCTGCCAGAACTGTGGCTCAACAAATATCAAGGTCCCTATCCAGATCCGCCACATTCAGTCACTCTCGCACCTTATGCACAGCCACTGCAGCAAGCGAGACACGGACCAAGAATCTTGAGCGCATTGCTGATGAGCTCTTGGACCTCTCAAAGATTGAGAGGCATGATTACTCCATCCTCTTCAGGCTCAAAATGGGCCTCAACAGGTATGGCCCCGCAATCTCAGGGATGAGTCCCGCATCTTCTGAATCTGGGCCTGCTTCCACAGACTCTAAGGTGGCGGAGAAGGTCGCATTTGATGTAAAGCTTGAGAAGTTTGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACAGATTTGGGACTAAAGGACGCTAAAGAGTTGGTGGAAAAGGCTCCTGTTGTGCTGAAGAAGGGTGTGACCAAAGAGGAGGCAGGGCCTATTGTTGACAAGCTTAAGGAGTTAGGTGCTACTGTGCTATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

203

Amino Acids

22.02

Weight (kDa)

9.61

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 135 - 201 3.8e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 118
AciI CCGC 5 cut(s) 156, 324, 345, 389, 434
AclWI GGATC 1 cut(s) 147
AcuI CTGAAG 2 cut(s) 274, 548
AfiI CCNNNNNNNGG 1 cut(s) 66
AflII CTTAAG 1 cut(s) 575
AgsI TTSAA 1 cut(s) 86
AluBI AGCT 3 cut(s) 244, 415, 574
AluI AGCT 3 cut(s) 244, 415, 574
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 147
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 4 cut(s) 304, 319, 362, 557
ApeKI GCWGC 2 cut(s) 197, 431
AspLEI GCGC 1 cut(s) 231
AspS9I GGNCC 8 cut(s) 69, 141, 213, 250, 304, 320, 362, 557
AsuHPI GGTGA 1 cut(s) 99
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 4 cut(s) 69, 141, 213, 250
AxyI CCTNAGG 1 cut(s) 64
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 209, 443
BccI CCATC 2 cut(s) 87, 290
BcoDI GTCTC 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 118
BisI GCNGC 3 cut(s) 198, 432, 435
BlsI GCNGC 3 cut(s) 199, 433, 436
Bme18I GGWCC 4 cut(s) 69, 141, 213, 250
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 8 cut(s) 69, 141, 213, 250, 304, 320, 362, 557
BmiI GGNNCC 4 cut(s) 70, 143, 322, 516
BmsI GCATC 1 cut(s) 356
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpuEI CTTGAG 2 cut(s) 245, 437
BsaBI GATNNNNATC 1 cut(s) 446
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse21I CCTNAGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 446
BseGI GGATG 2 cut(s) 282, 342
BseJI GATNNNNATC 1 cut(s) 446
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 2 cut(s) 209, 443
BshFI GGCC 4 cut(s) 306, 321, 364, 559
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 25
BslFI GGGAC 3 cut(s) 127, 327, 495
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 3 cut(s) 127, 327, 495
BsnI GGCC 4 cut(s) 306, 321, 364, 559
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 3 cut(s) 152, 441, 447
BspACI CCGC 5 cut(s) 156, 324, 345, 389, 434
BspANI GGCC 4 cut(s) 306, 321, 364, 559
BspCNI CTCAG 1 cut(s) 344
BspLI GGNNCC 4 cut(s) 70, 143, 322, 516
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 1 cut(s) 147
BspTI CTTAAG 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 231
BssMI GATC 3 cut(s) 152, 441, 447
Bst4CI ACNGT 2 cut(s) 121, 595
Bst6I CTCTTC 1 cut(s) 293
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 7, 202, 366
BstDEI CTNAG 3 cut(s) 64, 331, 381
BstF5I GGATG 2 cut(s) 282, 342
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 3 cut(s) 155, 444, 450
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 3 cut(s) 152, 441, 447
BstMWI GCNNNNNNNGC 2 cut(s) 183, 197
BstNSI RCATGY 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 209, 443
BstX2I RGATCY 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 4 cut(s) 306, 321, 364, 559
BtsCI GGATG 2 cut(s) 282, 342
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 3 cut(s) 7, 202, 366
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 1 cut(s) 231
Cfr13I GGNCC 8 cut(s) 69, 141, 213, 250, 304, 320, 362, 557
CseI GACGC 3 cut(s) 27, 437, 500
CviAII CATG 3 cut(s) 6, 32, 272
DdeI CTNAG 3 cut(s) 64, 331, 381
DpnI GATC 3 cut(s) 154, 443, 449
DpnII GATC 3 cut(s) 152, 441, 447
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 2 cut(s) 145, 404
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 69, 141, 213, 250
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 2 cut(s) 274, 548
Eco81I CCTNAGG 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 2 cut(s) 141, 557
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 3 cut(s) 9, 35, 275
FaiI YATR 7 cut(s) 7, 33, 98, 184, 273, 318, 452
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 3 cut(s) 127, 327, 495
FatI CATG 3 cut(s) 5, 31, 271
FauI CCCGC 2 cut(s) 331, 352
Fnu4HI GCNGC 3 cut(s) 198, 432, 435
FokI GGATG 2 cut(s) 269, 349
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 198, 432, 435
GlaI GCGC 1 cut(s) 230
GluI GCNGC 3 cut(s) 198, 432, 435
HaeIII GGCC 4 cut(s) 306, 321, 364, 559
HgaI GACGC 3 cut(s) 27, 437, 500
HhaI GCGC 1 cut(s) 231
Hin1II CATG 3 cut(s) 9, 35, 275
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 413, 572
HinfI GANTC 5 cut(s) 28, 220, 340, 356, 377
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 4 cut(s) 25, 150, 224, 445
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 2 cut(s) 388, 526
HpyCH4III ACNGT 2 cut(s) 121, 595
HpyCH4V TGCA 2 cut(s) 186, 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 197
HpyF3I CTNAG 3 cut(s) 64, 331, 381
Hsp92II CATG 3 cut(s) 9, 35, 275
HspAI GCGC 1 cut(s) 229
Kzo9I GATC 3 cut(s) 152, 441, 447
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 2 cut(s) 209, 443
LweI GCATC 1 cut(s) 356
MaeIII GTNAC 2 cut(s) 167, 538
MalI GATC 3 cut(s) 154, 443, 449
MboI GATC 3 cut(s) 152, 441, 447
MboII GAAGA 4 cut(s) 74, 280, 342, 541
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 37, 349, 371
MnlI CCTC 7 cut(s) 261, 263, 296, 317, 451, 541, 544
MseI TTAA 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 575
MwoI GCNNNNNNNGC 2 cut(s) 183, 197
NdeII GATC 3 cut(s) 152, 441, 447
NlaIII CATG 3 cut(s) 9, 35, 275
NlaIV GGNNCC 4 cut(s) 70, 143, 322, 516
NmuCI GTSAC 2 cut(s) 167, 538
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 220, 356
PkrI GCNGC 3 cut(s) 199, 433, 436
PleI GAGTC 3 cut(s) 36, 348, 371
PpsI GAGTC 3 cut(s) 36, 348, 371
PpuMI RGGWCCY 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 70, 143, 322, 516
PspPI GGNCC 8 cut(s) 69, 141, 213, 250, 304, 320, 362, 557
PspPPI RGGWCCY 1 cut(s) 141
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 152
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 3 cut(s) 198, 432, 435
Sau3AI GATC 3 cut(s) 152, 441, 447
Sau96I GGNCC 8 cut(s) 69, 141, 213, 250, 304, 320, 362, 557
SchI GAGTC 3 cut(s) 37, 349, 371
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 1 cut(s) 356
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 25
SinI GGWCC 4 cut(s) 69, 141, 213, 250
SlaI CTCGAG 1 cut(s) 25
SmlI CTYRAG 4 cut(s) 25, 224, 416, 575
SmoI CTYRAG 4 cut(s) 25, 224, 416, 575
SphI GCATGC 1 cut(s) 9
SsiI CCGC 5 cut(s) 156, 324, 345, 389, 434
SstI GAGCTC 1 cut(s) 246
TaaI ACNGT 2 cut(s) 121, 595
TaqI TCGA 1 cut(s) 26
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 2 cut(s) 220, 356
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 167, 538
TseI GCWGC 2 cut(s) 197, 431
Tsp45I GTSAC 2 cut(s) 167, 538
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 199
Vha464I CTTAAG 1 cut(s) 575
VpaK11BI GGWCC 4 cut(s) 69, 141, 213, 250
XceI RCATGY 1 cut(s) 9
XhoI CTCGAG 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.