pycom05g03280

Ribosomal protein L12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002385
Physical Location & Seq
Forward (+)
3811158 .. 3811511
354 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03280.1

Sequence Viewer

Length: 354 bp
ATGGGCCTCAATCGGTACGGACTAGCAATCTCGAGTATTGGATCAGCATCTTCTGACTCTGGGCCTGCTTCTATAGAATCCAAGGTTGCAAAAAAGATTGCATTTGATATAAAGATTGAGAAATTTGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGAATTGGGACTGAAGGAGGCTAAAGAATTAGTGAATAAGGTCTCTGTTGTGGTGAAGAAAGGGTTAACCAAGGCGAAGGAAGAGCCTATTGTCGATAAGCTAAAGGAATTGGGTGCTACTGTCATATTTTATTTCTCGATGGTGATTTTTGTGCTACTATTTCAGTCATCTGCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

118

Amino Acids

12.7

Weight (kDa)

9.55

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 35 - 99 1.4e-17 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 134
AclWI GGATC 1 cut(s) 49
AcsI RAATTY 1 cut(s) 122
AcuI CTGAAG 1 cut(s) 206
AfaI GTAC 1 cut(s) 17
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 2 cut(s) 4, 62
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 4, 62
AsuHPI GGTGA 2 cut(s) 238, 328
AvaI CYCGRG 1 cut(s) 31
BbvI GCAGC 1 cut(s) 143
BccI CCATC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 72
BisI GCNGC 2 cut(s) 132, 135
BlsI GCNGC 2 cut(s) 133, 136
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 4, 62
BmsI GCATC 1 cut(s) 56
BsaBI GATNNNNATC 2 cut(s) 46, 146
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 81, 243
Bse8I GATNNNNATC 2 cut(s) 46, 146
BseDI CCNNGG 2 cut(s) 81, 243
BseJI GATNNNNATC 2 cut(s) 46, 146
BseXI GCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 2 cut(s) 6, 64
BsiHKCI CYCGRG 1 cut(s) 31
BslFI GGGAC 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 2 cut(s) 6, 64
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 31
Bsp143I GATC 3 cut(s) 41, 141, 147
BspACI CCGC 1 cut(s) 134
BspANI GGCC 2 cut(s) 6, 64
BspPI GGATC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 81, 243
BssMI GATC 3 cut(s) 41, 141, 147
BssT1I CCWWGG 2 cut(s) 81, 243
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 66
BstKTI GATC 3 cut(s) 44, 144, 150
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 3 cut(s) 41, 141, 147
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 143
BsuRI GGCC 2 cut(s) 6, 64
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 66
Cfr13I GGNCC 2 cut(s) 4, 62
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 16
CviJI RGCY 5 cut(s) 6, 64, 194, 259, 274
CviKI_1 RGCY 5 cut(s) 6, 64, 194, 259, 274
CviQI GTAC 1 cut(s) 16
DpnI GATC 3 cut(s) 43, 143, 149
DpnII GATC 3 cut(s) 41, 141, 147
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 81, 243
Eco31I GGTCTC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 31
EcoT14I CCWWGG 2 cut(s) 81, 243
ErhI CCWWGG 2 cut(s) 81, 243
FaiI YATR 4 cut(s) 74, 110, 152, 299
FaqI GGGAC 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 132, 135
Fsp4HI GCNGC 2 cut(s) 132, 135
FspBI CTAG 1 cut(s) 23
GluI GCNGC 2 cut(s) 132, 135
HaeIII GGCC 2 cut(s) 6, 64
HgaI GACGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 2 cut(s) 56, 77
HpaI GTTAAC 1 cut(s) 240
HphI GGTGA 2 cut(s) 238, 328
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 3 cut(s) 31, 145, 310
Hpy8I GTNNAC 1 cut(s) 240
HpyAV CCTTC 2 cut(s) 181, 244
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 3 cut(s) 89, 101, 347
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 3 cut(s) 41, 141, 147
LguI GCTCTTC 1 cut(s) 249
LpnPI CCDG 2 cut(s) 45, 78
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 1 cut(s) 56
MaeI CTAG 1 cut(s) 23
MalI GATC 3 cut(s) 43, 143, 149
MboI GATC 3 cut(s) 41, 141, 147
MboII GAAGA 3 cut(s) 42, 241, 266
MluCI AATT 4 cut(s) 122, 176, 200, 281
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 3 cut(s) 17, 151, 184
MseI TTAA 2 cut(s) 239, 352
MspA1I CMGCKG 1 cut(s) 134
NdeII GATC 3 cut(s) 41, 141, 147
PaeR7I CTCGAG 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 133, 136
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PspPI GGNCC 2 cut(s) 4, 62
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 239, 352
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 3 cut(s) 41, 141, 147
Sau96I GGNCC 2 cut(s) 4, 62
SchI GAGTC 1 cut(s) 50
SetI ASST 4 cut(s) 87, 162, 216, 276
SfaNI GCATC 1 cut(s) 56
SfcI CTRYAG 1 cut(s) 72
Sfr274I CTCGAG 1 cut(s) 31
SgeI CNNG 9 cut(s) 35, 43, 45, 72, 77, 94, 157, 256, 322
SlaI CTCGAG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 4 cut(s) 122, 176, 200, 281
SsiI CCGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 2 cut(s) 81, 243
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 32, 267, 311
TasI AATT 4 cut(s) 122, 176, 200, 281
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 2 cut(s) 239, 352
Tru9I TTAA 2 cut(s) 239, 352
TscAI CASTG 1 cut(s) 178
TseI GCWGC 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 178
XapI RAATTY 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 31
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.