FvH4_5g26350

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002385
Physical Location & Seq
Forward (+)
17861736 .. 17865384
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26350.t2

Sequence Viewer

Length: 606 bp
ATGGCATGTCTGCGACACTTCCGGAGCCAAGCCCGTGTTTCTCAATCCATTACTACTATCTTAGGGTACAAAGAACATCTTCAAAGTAAGGCTGTAATACACTCACCTGTGAGGATTGTGGCTCAACAAATATCAGGGTCCCAATCCAGACCTGCCACATGCAGTCTTTCTCGCACCCTCTACACAGCTACTGGAGAAAGCAAGTCTCAGTCCAAGAATCTTGAGCGCATTGCTGAAGACCTCTTGGACCTCACAAAGATTGAGAGGCAAGACTACTCCATCCTCTTCAGGCTAAAAATGGGCCTCAACCAATATGGACCAGCAGTCTCAGGGATAGACCCAACATCTTCTGAATCTGGGTCTGCCTCCGCAGACTCCAAGGTTGTGGAGAAGACCGCATTTGACATAAAGCTTGAGAAGTTTGACGCATCTGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACGGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTGTGCTGAAAAAGGGTGTCACCAAAGAGGAGGCAGGGTCCATTGTTGACAAGCTCAAGGAGTTGGGTGCTACTGTGGTATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

202

Amino Acids

21.97

Weight (kDa)

9.24

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 134 - 200 1.1e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 2 cut(s) 369, 396
AcuI CTGAAG 2 cut(s) 255, 271
AfaI GTAC 1 cut(s) 68
AgsI TTSAA 1 cut(s) 83
AhdI GACNNNNNGTC 1 cut(s) 323
AluBI AGCT 3 cut(s) 188, 412, 571
AluI AGCT 3 cut(s) 188, 412, 571
Alw26I GTCTC 2 cut(s) 210, 331
AlwNI CAGNNNCTG 1 cut(s) 191
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 1 cut(s) 301
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 5 cut(s) 138, 247, 301, 317, 555
AsuHPI GGTGA 2 cut(s) 96, 529
AvaII GGWCC 4 cut(s) 138, 247, 317, 555
BbsI GAAGAC 2 cut(s) 243, 398
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 2 cut(s) 210, 331
BfuAI ACCTGC 1 cut(s) 160
Bme18I GGWCC 4 cut(s) 138, 247, 317, 555
BmeRI GACNNNNNGTC 1 cut(s) 323
BmgT120I GGNCC 5 cut(s) 138, 247, 301, 317, 555
BmiI GGNNCC 5 cut(s) 26, 139, 140, 513, 556
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 243, 398
BpmI CTGGAG 2 cut(s) 213, 498
BpuEI CTTGAG 3 cut(s) 242, 434, 557
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 378
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bse1I ACTGG 2 cut(s) 196, 515
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 443
BseAI TCCGGA 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 443
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 221, 342
BseNI ACTGG 2 cut(s) 196, 515
BseRI GAGGAG 1 cut(s) 560
BshFI GGCC 1 cut(s) 303
BsiSI CCGG 1 cut(s) 22
BslFI GGGAC 2 cut(s) 124, 492
BsmAI GTCTC 2 cut(s) 210, 331
BsmFI GGGAC 2 cut(s) 124, 492
BsnI GGCC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 2 cut(s) 438, 444
BspACI CCGC 2 cut(s) 369, 396
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 220, 341
BspEI TCCGGA 1 cut(s) 21
BspLI GGNNCC 5 cut(s) 26, 139, 140, 513, 556
BspMI ACCTGC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 196, 515
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 2 cut(s) 438, 444
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 1 cut(s) 290
BstDEI CTNAG 3 cut(s) 61, 207, 328
BstF5I GGATG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 441, 447
BstMAI GTCTC 2 cut(s) 210, 331
BstMBI GATC 2 cut(s) 438, 444
BstNSI RCATGY 2 cut(s) 9, 162
BstV2I GAAGAC 2 cut(s) 243, 398
BstXI CCANNNNNNTGG 1 cut(s) 385
BsuRI GGCC 1 cut(s) 303
BtsCI GGATG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 5 cut(s) 138, 247, 301, 317, 555
CseI GACGC 2 cut(s) 434, 497
Csp6I GTAC 1 cut(s) 67
CviAII CATG 2 cut(s) 6, 159
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 3 cut(s) 61, 207, 328
DpnI GATC 2 cut(s) 440, 446
DpnII GATC 2 cut(s) 438, 444
DriI GACNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 290
Eam1105I GACNNNNNGTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 378
Eco47I GGWCC 4 cut(s) 138, 247, 317, 555
Eco57I CTGAAG 2 cut(s) 255, 271
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 9, 162
FaiI YATR 5 cut(s) 7, 160, 315, 407, 449
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 2 cut(s) 124, 492
FatI CATG 2 cut(s) 5, 158
FokI GGATG 1 cut(s) 266
GlaI GCGC 1 cut(s) 227
GsuI CTGGAG 2 cut(s) 213, 498
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 22
HgaI GACGC 2 cut(s) 434, 497
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 9, 162
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 217, 353, 374
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 96, 529
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 4 cut(s) 22, 147, 221, 442
Hpy8I GTNNAC 1 cut(s) 565
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 2 cut(s) 162, 434
HpyF3I CTNAG 3 cut(s) 61, 207, 328
Hsp92II CATG 2 cut(s) 9, 162
HspAI GCGC 1 cut(s) 226
KflI GGGWCCC 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 2 cut(s) 438, 444
LmnI GCTCC 2 cut(s) 24, 517
LweI GCATC 1 cut(s) 437
MaeIII GTNAC 1 cut(s) 535
MalI GATC 2 cut(s) 440, 446
MboI GATC 2 cut(s) 438, 444
MboII GAAGA 5 cut(s) 71, 248, 277, 339, 403
MlyI GAGTC 1 cut(s) 368
MroI TCCGGA 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 78
MspI CCGG 1 cut(s) 22
NdeII GATC 2 cut(s) 438, 444
NlaIII CATG 2 cut(s) 9, 162
NlaIV GGNNCC 5 cut(s) 26, 139, 140, 513, 556
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 2 cut(s) 9, 162
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 217, 353
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
PspN4I GGNNCC 5 cut(s) 26, 139, 140, 513, 556
PspPI GGNCC 5 cut(s) 138, 247, 301, 317, 555
PspPPI RGGWCCY 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 191
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
Sau3AI GATC 2 cut(s) 438, 444
Sau96I GGNCC 5 cut(s) 138, 247, 301, 317, 555
SchI GAGTC 1 cut(s) 368
SetI ASST 9 cut(s) 109, 154, 190, 243, 252, 384, 414, 459, 573
SfaNI GCATC 1 cut(s) 437
SinI GGWCC 4 cut(s) 138, 247, 317, 555
SmlI CTYRAG 3 cut(s) 221, 413, 572
SmoI CTYRAG 3 cut(s) 221, 413, 572
SsiI CCGC 2 cut(s) 369, 396
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 1 cut(s) 592
TfiI GAWTC 2 cut(s) 217, 353
TseFI GTSAC 1 cut(s) 535
Tsp45I GTSAC 1 cut(s) 535
TspGWI ACGGA 1 cut(s) 485
VpaK11BI GGWCC 4 cut(s) 138, 247, 317, 555
XceI RCATGY 2 cut(s) 9, 162
XmnI GAANNNNTTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.