Rroxscaffold_7G00198910

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002385
Physical Location & Seq
Forward (+)
44680258 .. 44682564
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198910.1

Sequence Viewer

Length: 606 bp
ATGGCATGCTCTTTACGACGCTTCTCAAGTTATGCTCGTATTTCTCAAACCACTATTACCGCCTTAGGGTCCAAAGACCATCTTCAAAGCAAGGCTATAATACACTCACCTGCCAGAACTGTGGCTCAACAAATATCAGGGTCCCTATCCAGACCCGCCACATTCAGTCACTCTCGCACCCTCTGCTCAGCCACTGCAAGTGAGACACGGACCAAGAATCTTGAGCGCATTGCTGATGAGCTCTTGGACCTCTCAAAGATTGAGAGGCATGATTACTCCATCCTCTTCAGGCTCAAAATGGGCCTCAACAGGTATGGCCCCGCAATCTCAGGGATGAGTCCCGCATCTTCTGAATCTGGGCCTGCTTCCACAGACTCTAAGGTGGCGGAGAAGGTCGCATTTGATATAAAGCTAGAGAAGTTTGACGCGGCAGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGATTTGGGACTAAAGGACGCTAAAGAGTTGGTGGAAAAGGCTCCTGTTGTGCTGAAGAAGGGTGTGACCAAAGAGGAGGCAGGGCCTATTGTTGACAAGCTTAAGGAGTTAGGTGCTACTGTGGTATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

201

Amino Acids

21.85

Weight (kDa)

9.49

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 134 - 200 1e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 118
AccII CGCG 1 cut(s) 428
AciI CCGC 6 cut(s) 60, 156, 321, 342, 386, 428
AcuI CTGAAG 2 cut(s) 271, 545
AfiI CCNNNNNNNGG 1 cut(s) 66
AflII CTTAAG 1 cut(s) 572
AgsI TTSAA 1 cut(s) 86
AluBI AGCT 3 cut(s) 241, 412, 571
AluI AGCT 3 cut(s) 241, 412, 571
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 194
AoxI GGCC 4 cut(s) 301, 316, 359, 554
ApeKI GCWGC 1 cut(s) 431
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 8 cut(s) 69, 141, 210, 247, 301, 317, 359, 554
AsuHPI GGTGA 1 cut(s) 99
AvaII GGWCC 4 cut(s) 69, 141, 210, 247
AxyI CCTNAGG 1 cut(s) 64
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 443
BccI CCATC 2 cut(s) 87, 287
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 1 cut(s) 413
BfrI CTTAAG 1 cut(s) 572
BfuAI ACCTGC 1 cut(s) 118
BisI GCNGC 2 cut(s) 429, 432
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 2 cut(s) 430, 433
Bme18I GGWCC 4 cut(s) 69, 141, 210, 247
BmgT120I GGNCC 8 cut(s) 69, 141, 210, 247, 301, 317, 359, 554
BmiI GGNNCC 5 cut(s) 70, 142, 143, 319, 513
BmsI GCATC 1 cut(s) 353
Bpu1102I GCTNAGC 1 cut(s) 187
BpuEI CTTGAG 2 cut(s) 10, 242
BsaBI GATNNNNATC 1 cut(s) 443
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse21I CCTNAGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 443
BseGI GGATG 2 cut(s) 279, 339
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 201, 342
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 4 cut(s) 303, 318, 361, 556
BsiHKAI GWGCWC 1 cut(s) 243
BslFI GGGAC 3 cut(s) 127, 324, 492
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 3 cut(s) 127, 324, 492
BsnI GGCC 4 cut(s) 303, 318, 361, 556
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 438, 444
Bsp1720I GCTNAGC 1 cut(s) 187
BspACI CCGC 6 cut(s) 60, 156, 321, 342, 386, 428
BspANI GGCC 4 cut(s) 303, 318, 361, 556
BspCNI CTCAG 2 cut(s) 200, 341
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 5 cut(s) 70, 142, 143, 319, 513
BspMI ACCTGC 1 cut(s) 118
BspTI CTTAAG 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 228
BssMI GATC 2 cut(s) 438, 444
Bst4CI ACNGT 2 cut(s) 121, 592
Bst6I CTCTTC 1 cut(s) 290
BstAFI CTTAAG 1 cut(s) 572
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 7, 363
BstDEI CTNAG 4 cut(s) 64, 187, 328, 378
BstF5I GGATG 2 cut(s) 279, 339
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 441, 447
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 2 cut(s) 438, 444
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 9
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 1 cut(s) 443
BstXI CCANNNNNNTGG 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 4 cut(s) 303, 318, 361, 556
BtsCI GGATG 2 cut(s) 279, 339
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 192, 468
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 7, 363
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 8 cut(s) 69, 141, 210, 247, 301, 317, 359, 554
CseI GACGC 3 cut(s) 27, 434, 497
CviAII CATG 2 cut(s) 6, 269
DdeI CTNAG 4 cut(s) 64, 187, 328, 378
DpnI GATC 2 cut(s) 440, 446
DpnII GATC 2 cut(s) 438, 444
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 4 cut(s) 69, 141, 210, 247
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 271, 545
Eco81I CCTNAGG 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 141, 554
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 2 cut(s) 9, 272
FaiI YATR 7 cut(s) 7, 33, 98, 270, 315, 407, 449
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 3 cut(s) 127, 324, 492
FatI CATG 2 cut(s) 5, 268
FauI CCCGC 3 cut(s) 163, 328, 349
Fnu4HI GCNGC 2 cut(s) 429, 432
FokI GGATG 2 cut(s) 266, 346
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 429, 432
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 429, 432
HaeIII GGCC 4 cut(s) 303, 318, 361, 556
HgaI GACGC 3 cut(s) 27, 434, 497
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 9, 272
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 4 cut(s) 217, 337, 353, 374
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 150, 221, 442
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 2 cut(s) 385, 523
HpyCH4III ACNGT 2 cut(s) 121, 592
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 4 cut(s) 64, 187, 328, 378
Hsp92II CATG 2 cut(s) 9, 272
HspAI GCGC 1 cut(s) 226
KflI GGGWCCC 1 cut(s) 141
Kzo9I GATC 2 cut(s) 438, 444
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 1 cut(s) 353
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 167, 535
MalI GATC 2 cut(s) 440, 446
MboI GATC 2 cut(s) 438, 444
MboII GAAGA 4 cut(s) 74, 277, 339, 538
MhlI GDGCHC 1 cut(s) 243
MlyI GAGTC 2 cut(s) 346, 368
MnlI CCTC 8 cut(s) 191, 258, 260, 293, 314, 448, 538, 541
MseI TTAA 1 cut(s) 573
MspCI CTTAAG 1 cut(s) 572
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 183
NdeII GATC 2 cut(s) 438, 444
NlaIII CATG 2 cut(s) 9, 272
NlaIV GGNNCC 5 cut(s) 70, 142, 143, 319, 513
NmuCI GTSAC 2 cut(s) 167, 535
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PaqCI CACCTGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 217, 353
PkrI GCNGC 2 cut(s) 430, 433
PleI GAGTC 2 cut(s) 345, 368
PpsI GAGTC 2 cut(s) 345, 368
PpuMI RGGWCCY 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 141
PspN4I GGNNCC 5 cut(s) 70, 142, 143, 319, 513
PspPI GGNCC 8 cut(s) 69, 141, 210, 247, 301, 317, 359, 554
PspPPI RGGWCCY 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 194
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 2 cut(s) 429, 432
Sau3AI GATC 2 cut(s) 438, 444
Sau96I GGNCC 8 cut(s) 69, 141, 210, 247, 301, 317, 359, 554
SchI GAGTC 2 cut(s) 346, 368
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 1 cut(s) 353
SinI GGWCC 4 cut(s) 69, 141, 210, 247
SmlI CTYRAG 3 cut(s) 25, 221, 572
SmoI CTYRAG 3 cut(s) 25, 221, 572
SphI GCATGC 1 cut(s) 9
SsiI CCGC 6 cut(s) 60, 156, 321, 342, 386, 428
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 243
TaaI ACNGT 2 cut(s) 121, 592
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 2 cut(s) 217, 353
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 2 cut(s) 199, 475
TseFI GTSAC 2 cut(s) 167, 535
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 2 cut(s) 167, 535
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 2 cut(s) 199, 475
Vha464I CTTAAG 1 cut(s) 572
VpaK11BI GGWCC 4 cut(s) 69, 141, 210, 247
XceI RCATGY 1 cut(s) 9
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.