Rh7AG305500

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002385
Physical Location & Seq
Forward (+)
34093697 .. 34097633
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG305500.1

Sequence Viewer

Length: 480 bp
ATGTCCCAAAAGTCCCAATCCAGACCTGCCACGTGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCCTCGGTCCAAGAATCTTGAGTGCATTGCTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAGGTTAAAAATGGGCCTCAACCGATATAGTCCAGCAGTCTCAGGGATAGGCCCAACATCCTCCGAATCTGGGTCTGCCTCCACAGACTCCAAGGTGGTGGAGAAGACCGCATTTGATATAAAGCTTGAGAAGTTTGACGCAGTGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAGAAGGGTGTCACCAAAGAGGAGGCAGGCCCCATTGTTGACAAGCTCAAGGAGTTGGGTGCTACTGTGGTACTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

159

Amino Acids

17.32

Weight (kDa)

9.0

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 92 - 158 1.5e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AciI CCGC 1 cut(s) 270
AcuI CTGAAG 2 cut(s) 145, 419
AcvI CACGTG 1 cut(s) 33
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 1 cut(s) 231
AluBI AGCT 2 cut(s) 286, 445
AluI AGCT 2 cut(s) 286, 445
Alw26I GTCTC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 3 cut(s) 175, 211, 427
ApeKI GCWGC 1 cut(s) 68
AspS9I GGNCC 5 cut(s) 84, 121, 175, 212, 428
AsuHPI GGTGA 1 cut(s) 403
AvaII GGWCC 2 cut(s) 84, 121
BbrPI CACGTG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme18I GGWCC 2 cut(s) 84, 121
BmgT120I GGNCC 5 cut(s) 84, 121, 175, 212, 428
BmiI GGNNCC 2 cut(s) 387, 430
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 372
BpuEI CTTGAG 3 cut(s) 116, 308, 431
BsaAI YACGTR 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 80, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 389, 477
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 80, 252
BseGI GGATG 2 cut(s) 153, 218
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 389, 477
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 55
BshFI GGCC 3 cut(s) 177, 213, 429
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 3 cut(s) 177, 213, 429
Bsp143I GATC 2 cut(s) 312, 318
BspACI CCGC 1 cut(s) 270
BspANI GGCC 3 cut(s) 177, 213, 429
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 387, 430
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 2 cut(s) 389, 477
BssECI CCNNGG 2 cut(s) 80, 252
BssMI GATC 2 cut(s) 312, 318
BssT1I CCWWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 153, 218
BstKTI GATC 2 cut(s) 315, 321
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 312, 318
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 3 cut(s) 177, 213, 429
BtsCI GGATG 2 cut(s) 153, 218
BtsI GCAGTG 2 cut(s) 63, 309
BtsIMutI CAGTG 3 cut(s) 63, 309, 342
BveI ACCTGC 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 5 cut(s) 84, 121, 175, 212, 428
CseI GACGC 2 cut(s) 308, 371
Csp6I GTAC 1 cut(s) 470
CviAII CATG 1 cut(s) 143
CviJI RGCY 8 cut(s) 62, 79, 177, 213, 286, 386, 429, 445
CviKI_1 RGCY 8 cut(s) 62, 79, 177, 213, 286, 386, 429, 445
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 1 cut(s) 202
DpnI GATC 2 cut(s) 314, 320
DpnII GATC 2 cut(s) 312, 318
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 84, 121
Eco57I CTGAAG 2 cut(s) 145, 419
Eco72I CACGTG 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 146
FaiI YATR 4 cut(s) 144, 189, 281, 323
FaqI GGGAC 1 cut(s) 366
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 140, 205
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 3 cut(s) 177, 213, 429
HgaI GACGC 2 cut(s) 308, 371
Hin1II CATG 1 cut(s) 146
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 91, 227, 248
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 3 cut(s) 21, 95, 316
Hpy8I GTNNAC 1 cut(s) 439
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 36, 68, 102
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 202
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 1 cut(s) 146
Kzo9I GATC 2 cut(s) 312, 318
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 9 cut(s) 34, 39, 148, 189, 207, 216, 402, 411, 458
Lsp1109I GCAGC 1 cut(s) 80
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 409
MalI GATC 2 cut(s) 314, 320
MboI GATC 2 cut(s) 312, 318
MboII GAAGA 3 cut(s) 151, 277, 412
MlyI GAGTC 1 cut(s) 242
MseI TTAA 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 2 cut(s) 312, 318
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 2 cut(s) 387, 430
NmuCI GTSAC 1 cut(s) 409
PfeI GAWTC 2 cut(s) 91, 227
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 242
PmaCI CACGTG 1 cut(s) 33
PmlI CACGTG 1 cut(s) 33
PpsI GAGTC 1 cut(s) 242
Ppu21I YACGTR 1 cut(s) 33
PspCI CACGTG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 387, 430
PspPI GGNCC 5 cut(s) 84, 121, 175, 212, 428
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 312, 318
Sau96I GGNCC 5 cut(s) 84, 121, 175, 212, 428
SchI GAGTC 1 cut(s) 242
SetI ASST 9 cut(s) 28, 35, 117, 126, 167, 258, 288, 333, 447
SfcI CTRYAG 1 cut(s) 66
SinI GGWCC 2 cut(s) 84, 121
SmlI CTYRAG 3 cut(s) 95, 287, 446
SmoI CTYRAG 3 cut(s) 95, 287, 446
SsiI CCGC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 35
TaqII GACCGA 1 cut(s) 72
TfiI GAWTC 2 cut(s) 91, 227
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 3 cut(s) 70, 309, 349
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 409
TspRI CASTG 3 cut(s) 70, 309, 349
VpaK11BI GGWCC 2 cut(s) 84, 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.