RchiOBHm_Chr7g0217161

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002385
Physical Location & Seq
Forward (+)
35047335 .. 35047939
605 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19433

Sequence Viewer

Length: 558 bp
ATGAATTTCTGTCGCACAAAACCCCAACCCTTTTATCTTTCCAGAACGTCCTTCACCACAAATGTTCCCTGCATGTCCCAATCCAGACCTGCCTCGTGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCCTCGATCCAAGAATCTTGAGCGCATTACTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAAACTTAAAATGGGCCTCAACAGATATGGTCCAGCTGTCTCAGGAATAGGCCCTTCATCTTCCGAATCTGGGTCTGCCTCCACAGGCTCCAAGGTGGTGGTGAAGACCGCATTTGATATAAAGCTTGAGAAGTTTGACGGAGCGGCAAAGATCAAAATCATAAAGGAGGTTAGGACTTTCATTGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTTTGCTAAAGATGGGTGTCACCAGAGAGGAGGCAGGCCTCATTGTTGACAGGCTGAAGGAGTTGGGTGCTACTGTGAAATGTGATCGATGTGTTCAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

185

Amino Acids

20.35

Weight (kDa)

9.49

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 113 - 178 4.7e-20 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 556
Acc36I ACCTGC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 368
AciI CCGC 2 cut(s) 333, 368
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 530
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 2 cut(s) 226, 551
AluBI AGCT 2 cut(s) 260, 349
AluI AGCT 2 cut(s) 260, 349
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 3 cut(s) 238, 274, 490
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 4 cut(s) 184, 238, 254, 275
AsuHPI GGTGA 3 cut(s) 46, 337, 466
AvaII GGWCC 2 cut(s) 184, 254
BauI CACGAG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 143
BccI CCATC 2 cut(s) 224, 460
BcoDI GTCTC 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 132, 369
BlsI GCNGC 2 cut(s) 133, 370
Bme18I GGWCC 2 cut(s) 184, 254
BmgT120I GGNCC 4 cut(s) 184, 238, 254, 275
BmiI GGNNCC 2 cut(s) 313, 450
BpiI GAAGAC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 179, 371
Bsa29I ATCGAT 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 452
Bse8I GATNNNNATC 1 cut(s) 380
BseCI ATCGAT 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 118
BshFI GGCC 3 cut(s) 240, 276, 492
BshVI ATCGAT 1 cut(s) 541
BslFI GGGAC 2 cut(s) 61, 429
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 2 cut(s) 61, 429
BsnI GGCC 3 cut(s) 240, 276, 492
Bsp143I GATC 3 cut(s) 146, 375, 538
BspACI CCGC 2 cut(s) 333, 368
BspANI GGCC 3 cut(s) 240, 276, 492
BspCNI CTCAG 1 cut(s) 278
BspDI ATCGAT 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 313, 450
BspMAI CTGCAG 1 cut(s) 133
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 368
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 146, 375, 538
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 216
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 149, 378, 541
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 3 cut(s) 146, 375, 538
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 322
Bsu15I ATCGAT 1 cut(s) 541
BsuRI GGCC 3 cut(s) 240, 276, 492
BsuTUI ATCGAT 1 cut(s) 541
BtsCI GGATG 1 cut(s) 216
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 490
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 4 cut(s) 184, 238, 254, 275
ClaI ATCGAT 1 cut(s) 541
CseI GACGC 1 cut(s) 434
CviAII CATG 2 cut(s) 73, 206
DdeI CTNAG 1 cut(s) 265
DpnI GATC 3 cut(s) 148, 377, 540
DpnII GATC 3 cut(s) 146, 375, 538
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 315
Eco147I AGGCCT 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 184, 254
Eco57I CTGAAG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 76, 209
FaiI YATR 6 cut(s) 74, 207, 252, 344, 386, 556
FaqI GGGAC 2 cut(s) 61, 429
FatI CATG 2 cut(s) 72, 205
Fnu4HI GCNGC 2 cut(s) 132, 369
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 132, 369
GlaI GCGC 1 cut(s) 164
GluI GCNGC 2 cut(s) 132, 369
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 3 cut(s) 240, 276, 492
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 165
Hin1II CATG 2 cut(s) 76, 209
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 2 cut(s) 154, 290
HphI GGTGA 3 cut(s) 46, 337, 466
Hpy166II GTNNAC 1 cut(s) 502
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 4 cut(s) 42, 84, 158, 267
Hpy8I GTNNAC 1 cut(s) 502
HpyAV CCTTC 3 cut(s) 61, 288, 505
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 3 cut(s) 72, 99, 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 1 cut(s) 47
Hsp92II CATG 2 cut(s) 76, 209
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 3 cut(s) 146, 375, 538
LmnI GCTCC 3 cut(s) 317, 365, 454
Lsp1109I GCAGC 1 cut(s) 143
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 1 cut(s) 472
MalI GATC 3 cut(s) 148, 377, 540
MbiI CCGCTC 1 cut(s) 368
MboI GATC 3 cut(s) 146, 375, 538
MboII GAAGA 3 cut(s) 214, 276, 340
MluCI AATT 2 cut(s) 4, 551
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeII GATC 3 cut(s) 146, 375, 538
NlaIII CATG 2 cut(s) 76, 209
NlaIV GGNNCC 2 cut(s) 313, 450
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 1 cut(s) 76
PceI AGGCCT 1 cut(s) 492
PfeI GAWTC 2 cut(s) 154, 290
PkrI GCNGC 2 cut(s) 133, 370
PsiI TTATAA 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 313, 450
PspPI GGNCC 4 cut(s) 184, 238, 254, 275
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 132, 369
Sau3AI GATC 3 cut(s) 146, 375, 538
Sau96I GGNCC 4 cut(s) 184, 238, 254, 275
SetI ASST 8 cut(s) 50, 91, 180, 189, 262, 321, 351, 396
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 2 cut(s) 184, 254
SmlI CTYRAG 2 cut(s) 158, 350
SmoI CTYRAG 2 cut(s) 158, 350
Sse9I AATT 2 cut(s) 4, 551
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 2 cut(s) 333, 368
StuI AGGCCT 1 cut(s) 492
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 145, 541
TasI AATT 2 cut(s) 4, 551
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 2 cut(s) 154, 290
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 3 cut(s) 17, 270, 394
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 184, 254
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.