pycom04g03680

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002385
Physical Location & Seq
Forward (+)
3294997 .. 3295966
970 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03680.1

Sequence Viewer

Length: 552 bp
ATGGCGAGCTCACTACAACGCTTCTCCACTCATGCTAGGGCTGTAAAATGGTCACCTGCCAGAACTGCAGCTAAACAAGGATCAGGGTCCCTATCCACATTGCCTGCTACTGTTTCTCGCAACCTCACCACAGCAACTGCAGCAAATGAGACTCAAACGCATAAACTTGAGCGCATTGCCAACGAGCTTCTCGACCTCACAAAGCTTGAGAGGCACGACTACTCCATTCTCTTTAGGCTGAAAATGGGCCTCAACCGGTATGGCCCGGCAGTCTCAGGGATTGGCCCGGCAGCTTCCGAGTCTGGGCCGGCCTCCACGGCTGCCAAGGTCGCTGAGAAGACCGCATTTGATATAAAGATTGAGAAGTTTGATGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACGGACTTGGGGCTGAAGGAGGCTAAGGAGTTGGTGGAGAAGGTCCCTGTTGTGGTGAAGAAGGGCATGACGAAAGAGGAGGCAGGGCCCATTGTTGACAAGCTTAAGGAGTTGGGCGCTACTGTGGTCCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

184

Amino Acids

19.69

Weight (kDa)

9.67

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 116 - 182 5.7e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 64
AciI CCGC 2 cut(s) 342, 377
AclWI GGATC 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 303, 466
AflII CTTAAG 1 cut(s) 518
AgeI ACCGGT 1 cut(s) 255
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 6 cut(s) 9, 71, 187, 205, 293, 517
AluI AGCT 6 cut(s) 9, 71, 187, 205, 293, 517
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 2 cut(s) 143, 277
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 6 cut(s) 247, 262, 283, 305, 309, 500
ApaI GGGCCC 1 cut(s) 504
ApeKI GCWGC 5 cut(s) 68, 140, 290, 320, 374
AsiGI ACCGGT 1 cut(s) 255
AspLEI GCGC 2 cut(s) 174, 533
AspS9I GGNCC 9 cut(s) 87, 247, 263, 284, 305, 457, 500, 501, 541
AsuC2I CCSGG 2 cut(s) 266, 287
AsuHPI GGTGA 3 cut(s) 45, 118, 481
AvaII GGWCC 3 cut(s) 87, 457, 541
BaeGI GKGCMC 1 cut(s) 504
BanII GRGCYC 2 cut(s) 11, 504
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 5 cut(s) 80, 152, 302, 307, 386
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 545
BcnI CCSGG 2 cut(s) 266, 287
BcoDI GTCTC 2 cut(s) 143, 277
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 2 cut(s) 66, 138
BfoI RGCGCY 1 cut(s) 534
BfrI CTTAAG 1 cut(s) 518
BfuAI ACCTGC 1 cut(s) 64
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 6 cut(s) 69, 141, 291, 321, 375, 378
BlsI GCNGC 6 cut(s) 70, 142, 292, 322, 376, 379
Bme1390I CCNGG 3 cut(s) 266, 287, 545
Bme18I GGWCC 3 cut(s) 87, 457, 541
BmgT120I GGNCC 9 cut(s) 87, 247, 263, 284, 305, 457, 500, 501, 541
BmiI GGNNCC 4 cut(s) 88, 89, 459, 502
BmrFI CCNGG 3 cut(s) 266, 287, 545
BmsI GCATC 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 344
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 2 cut(s) 188, 227
BpuMI CCSGG 2 cut(s) 266, 287
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 315, 324
BsaWI WCCGGW 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 466
Bse118I RCCGGY 2 cut(s) 255, 307
Bse3DI GCAATG 2 cut(s) 98, 174
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 2 cut(s) 315, 324
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 303, 466
BseMI GCAATG 2 cut(s) 98, 174
BseMII CTCAG 2 cut(s) 288, 324
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 5 cut(s) 80, 152, 302, 307, 386
BshFI GGCC 6 cut(s) 249, 264, 285, 307, 311, 502
BshTI ACCGGT 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 4 cut(s) 256, 266, 287, 308
BslFI GGGAC 2 cut(s) 73, 443
BslI CCNNNNNNNGG 2 cut(s) 303, 466
BsmAI GTCTC 2 cut(s) 143, 277
BsmFI GGGAC 2 cut(s) 73, 443
BsnI GGCC 6 cut(s) 249, 264, 285, 307, 311, 502
Bsp120I GGGCCC 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 11, 504
Bsp143I GATC 3 cut(s) 80, 384, 390
BspACI CCGC 2 cut(s) 342, 377
BspANI GGCC 6 cut(s) 249, 264, 285, 307, 311, 502
BspCNI CTCAG 2 cut(s) 287, 325
BspLI GGNNCC 4 cut(s) 88, 89, 459, 502
BspMAI CTGCAG 2 cut(s) 70, 142
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 1 cut(s) 88
BspTI CTTAAG 1 cut(s) 518
BsrDI GCAATG 2 cut(s) 98, 174
BsrFI RCCGGY 2 cut(s) 255, 307
BssAI RCCGGY 2 cut(s) 255, 307
BssECI CCNNGG 2 cut(s) 315, 324
BssMI GATC 3 cut(s) 80, 384, 390
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 2 cut(s) 112, 538
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 7, 105, 309
BstDEI CTNAG 3 cut(s) 274, 333, 438
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 2 cut(s) 174, 533
BstKTI GATC 3 cut(s) 83, 387, 393
BstMAI GTCTC 2 cut(s) 143, 277
BstMBI GATC 3 cut(s) 80, 384, 390
BstMWI GCNNNNNNNGC 5 cut(s) 65, 140, 211, 317, 329
BstNI CCWGG 1 cut(s) 545
BstPI GGTNACC 1 cut(s) 51
BstSCI CCNGG 3 cut(s) 264, 285, 543
BstSFI CTRYAG 2 cut(s) 66, 138
BstSLI GKGCMC 1 cut(s) 504
BstV1I GCAGC 5 cut(s) 80, 152, 302, 307, 386
BstV2I GAAGAC 1 cut(s) 344
BsuRI GGCC 6 cut(s) 249, 264, 285, 307, 311, 502
BtgI CCRYGG 1 cut(s) 315
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 7, 105, 309
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 2 cut(s) 174, 533
Cfr10I RCCGGY 2 cut(s) 255, 307
Cfr13I GGNCC 9 cut(s) 87, 247, 263, 284, 305, 457, 500, 501, 541
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 2 cut(s) 32, 481
DdeI CTNAG 3 cut(s) 274, 333, 438
DpnI GATC 3 cut(s) 82, 386, 392
DpnII GATC 3 cut(s) 80, 384, 390
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 2 cut(s) 11, 504
Eco47I GGWCC 3 cut(s) 87, 457, 541
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 3 cut(s) 87, 457, 500
EcoO65I GGTNACC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 543
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 11, 504
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 2 cut(s) 35, 484
FaiI YATR 6 cut(s) 33, 162, 261, 353, 395, 482
FaqI GGGAC 2 cut(s) 73, 443
FatI CATG 2 cut(s) 31, 480
Fnu4HI GCNGC 6 cut(s) 69, 141, 291, 321, 375, 378
FriOI GRGCYC 2 cut(s) 11, 504
FseI GGCCGGCC 1 cut(s) 311
Fsp4HI GCNGC 6 cut(s) 69, 141, 291, 321, 375, 378
FspBI CTAG 1 cut(s) 36
GlaI GCGC 2 cut(s) 173, 532
GluI GCNGC 6 cut(s) 69, 141, 291, 321, 375, 378
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 6 cut(s) 249, 264, 285, 307, 311, 502
HapII CCGG 4 cut(s) 256, 266, 287, 308
HhaI GCGC 2 cut(s) 174, 533
Hin1II CATG 2 cut(s) 35, 484
Hin6I GCGC 2 cut(s) 172, 531
HinP1I GCGC 2 cut(s) 172, 531
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 203, 515
HinfI GANTC 2 cut(s) 151, 299
HpaII CCGG 4 cut(s) 256, 266, 287, 308
HphI GGTGA 3 cut(s) 45, 118, 481
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 191, 388
Hpy8I GTNNAC 1 cut(s) 511
HpyAV CCTTC 3 cut(s) 424, 448, 469
HpyCH4III ACNGT 2 cut(s) 112, 538
HpyCH4V TGCA 3 cut(s) 68, 140, 374
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 140, 211, 317, 329
HpyF3I CTNAG 3 cut(s) 274, 333, 438
Hsp92II CATG 2 cut(s) 35, 484
HspAI GCGC 2 cut(s) 172, 531
KflI GGGWCCC 1 cut(s) 87
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 3 cut(s) 80, 384, 390
Lsp1109I GCAGC 5 cut(s) 80, 152, 302, 307, 386
LweI GCATC 1 cut(s) 361
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 51
MalI GATC 3 cut(s) 82, 386, 392
MboI GATC 3 cut(s) 80, 384, 390
MboII GAAGA 2 cut(s) 349, 484
MhlI GDGCHC 2 cut(s) 11, 504
MlyI GAGTC 2 cut(s) 145, 308
MnlI CCTC 9 cut(s) 134, 204, 206, 260, 322, 394, 427, 484, 487
MroNI GCCGGC 1 cut(s) 307
MseI TTAA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 4 cut(s) 256, 266, 287, 308
MspR9I CCNGG 3 cut(s) 266, 287, 545
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 5 cut(s) 65, 140, 211, 317, 329
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 2 cut(s) 266, 287
NdeII GATC 3 cut(s) 80, 384, 390
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 35, 484
NlaIV GGNNCC 4 cut(s) 88, 89, 459, 502
NmuCI GTSAC 1 cut(s) 51
PaqCI CACCTGC 1 cut(s) 64
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdiI GCCGGC 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 543
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 6 cut(s) 70, 142, 292, 322, 376, 379
PleI GAGTC 2 cut(s) 145, 307
PpsI GAGTC 2 cut(s) 145, 307
PpuMI RGGWCCY 2 cut(s) 87, 457
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 2 cut(s) 87, 457
Psp6I CCWGG 1 cut(s) 543
PspEI GGTNACC 1 cut(s) 51
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 4 cut(s) 88, 89, 459, 502
PspOMI GGGCCC 1 cut(s) 500
PspPI GGNCC 9 cut(s) 87, 247, 263, 284, 305, 457, 500, 501, 541
PspPPI RGGWCCY 2 cut(s) 87, 457
PstI CTGCAG 2 cut(s) 70, 142
PstNI CAGNNNCTG 1 cut(s) 137
RigI GGCCGGCC 1 cut(s) 311
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 6 cut(s) 69, 141, 291, 321, 375, 378
Sau3AI GATC 3 cut(s) 80, 384, 390
Sau96I GGNCC 9 cut(s) 87, 247, 263, 284, 305, 457, 500, 501, 541
SchI GAGTC 2 cut(s) 145, 308
ScrFI CCNGG 3 cut(s) 266, 287, 545
SduI GDGCHC 2 cut(s) 11, 504
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 2 cut(s) 66, 138
SinI GGWCC 3 cut(s) 87, 457, 541
SmlI CTYRAG 3 cut(s) 167, 206, 518
SmoI CTYRAG 3 cut(s) 167, 206, 518
SsiI CCGC 2 cut(s) 342, 377
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 264, 285, 543
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 112, 538
TaqI TCGA 1 cut(s) 192
TauI GCSGC 1 cut(s) 380
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 5 cut(s) 68, 140, 290, 320, 374
Tsp45I GTSAC 1 cut(s) 51
TspGWI ACGGA 1 cut(s) 431
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 3 cut(s) 87, 457, 541
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.