Rw7G025950

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002385
Physical Location & Seq
Forward (+)
32810078 .. 32812020
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025950.1

Sequence Viewer

Length: 606 bp
ATGTCATCTCTACGACGCTTCTCAAGCCATGCTTGTGTTTCTCAAACCATTACTACTATCGTAGGGTCCAAAGTACATCTTCAAAGTAAGGCTGTAATACACTCACCTGCCAGAACTGTGGCTCAACAAATATCAGGGTCCCAATCCAGACCTGCCACGTGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCCTCGGTCCAAGAATCTTGAGTGCATTGCTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAGGTTAAAAATGGGCCTCAACCGATATGGTCCAGCAGTCTCAGGAATAGGCCCGACATCCTCCGAATCTGGGTCTGCCTCCACAGACTCCAAGGTGGTGGAGAAGATCGCATTTGATATAAAGCTTGAGAAGTTTGACGCAGTGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAGAAGGGTGTCACCAAGGAGGAGGCAGGCCCCATTGTTGACAAGCTCAAGGAGTTGGGTGCTACTGTGGTACTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

201

Amino Acids

21.71

Weight (kDa)

9.42

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 134 - 200 2.4e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
Acc36I ACCTGC 2 cut(s) 115, 160
AcuI CTGAAG 2 cut(s) 271, 545
AcvI CACGTG 1 cut(s) 159
AfaI GTAC 2 cut(s) 75, 597
AfiI CCNNNNNNNGG 1 cut(s) 357
AgsI TTSAA 1 cut(s) 83
AluBI AGCT 2 cut(s) 412, 571
AluI AGCT 2 cut(s) 412, 571
Alw26I GTCTC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 3 cut(s) 301, 337, 553
ApeKI GCWGC 1 cut(s) 194
AspS9I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
AsuHPI GGTGA 2 cut(s) 96, 529
AvaII GGWCC 5 cut(s) 66, 138, 210, 247, 317
BbrPI CACGTG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 206
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 2 cut(s) 115, 160
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme18I GGWCC 5 cut(s) 66, 138, 210, 247, 317
BmgT120I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
BmiI GGNNCC 5 cut(s) 67, 139, 140, 513, 556
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 4 cut(s) 7, 242, 434, 557
BsaAI YACGTR 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 206, 378, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 2 cut(s) 515, 603
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 206, 378, 540
BseGI GGATG 2 cut(s) 279, 344
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 515, 603
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 3 cut(s) 303, 339, 555
BslFI GGGAC 2 cut(s) 124, 492
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 124, 492
BsnI GGCC 3 cut(s) 303, 339, 555
Bsp143I GATC 3 cut(s) 393, 438, 444
BspANI GGCC 3 cut(s) 303, 339, 555
BspCNI CTCAG 1 cut(s) 341
BspLI GGNNCC 5 cut(s) 67, 139, 140, 513, 556
BspMAI CTGCAG 1 cut(s) 196
BspMI ACCTGC 2 cut(s) 115, 160
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 515, 603
BssECI CCNNGG 3 cut(s) 206, 378, 540
BssMI GATC 3 cut(s) 393, 438, 444
BssT1I CCWWGG 2 cut(s) 378, 540
Bst4CI ACNGT 2 cut(s) 118, 592
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 1 cut(s) 328
BstF5I GGATG 2 cut(s) 279, 344
BstKTI GATC 3 cut(s) 396, 441, 447
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 3 cut(s) 393, 438, 444
BstMWI GCNNNNNNNGC 2 cut(s) 24, 194
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 206
BstXI CCANNNNNNTGG 2 cut(s) 118, 385
BsuRI GGCC 3 cut(s) 303, 339, 555
BtsCI GGATG 2 cut(s) 279, 344
BtsI GCAGTG 2 cut(s) 189, 435
BtsIMutI CAGTG 3 cut(s) 189, 435, 468
BveI ACCTGC 2 cut(s) 115, 160
Cac8I GCNNGC 1 cut(s) 553
CaiI CAGNNNCTG 1 cut(s) 191
Cfr13I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
CseI GACGC 3 cut(s) 24, 434, 497
Csp6I GTAC 2 cut(s) 74, 596
CviAII CATG 2 cut(s) 29, 269
CviQI GTAC 2 cut(s) 74, 596
DdeI CTNAG 1 cut(s) 328
DpnI GATC 3 cut(s) 395, 440, 446
DpnII GATC 3 cut(s) 393, 438, 444
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 2 cut(s) 378, 540
Eco47I GGWCC 5 cut(s) 66, 138, 210, 247, 317
Eco57I CTGAAG 2 cut(s) 271, 545
Eco72I CACGTG 1 cut(s) 159
EcoO109I RGGNCCY 2 cut(s) 138, 554
EcoT14I CCWWGG 2 cut(s) 378, 540
ErhI CCWWGG 2 cut(s) 378, 540
FaeI CATG 2 cut(s) 32, 272
FaiI YATR 5 cut(s) 30, 270, 315, 407, 449
FalI AAGNNNNNCTT 4 cut(s) 16, 48, 63, 95
FaqI GGGAC 2 cut(s) 124, 492
FatI CATG 2 cut(s) 28, 268
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 2 cut(s) 266, 331
Fsp4HI GCNGC 1 cut(s) 195
GluI GCNGC 1 cut(s) 195
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 3 cut(s) 303, 339, 555
HgaI GACGC 3 cut(s) 24, 434, 497
Hin1II CATG 2 cut(s) 32, 272
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 217, 353, 374
HphI GGTGA 2 cut(s) 96, 529
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 4 cut(s) 147, 221, 330, 442
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 2 cut(s) 118, 592
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 3 cut(s) 162, 194, 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 194
HpyF3I CTNAG 1 cut(s) 328
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 2 cut(s) 32, 272
KflI GGGWCCC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 393, 438, 444
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 1 cut(s) 206
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 535
MalI GATC 3 cut(s) 395, 440, 446
MboI GATC 3 cut(s) 393, 438, 444
MboII GAAGA 4 cut(s) 71, 277, 403, 538
MlyI GAGTC 1 cut(s) 368
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 24, 194
NdeII GATC 3 cut(s) 393, 438, 444
NlaIII CATG 2 cut(s) 32, 272
NlaIV GGNNCC 5 cut(s) 67, 139, 140, 513, 556
NmuCI GTSAC 1 cut(s) 535
PaqCI CACCTGC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 217, 353
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 1 cut(s) 368
PmaCI CACGTG 1 cut(s) 159
PmlI CACGTG 1 cut(s) 159
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
PspCI CACGTG 1 cut(s) 159
PspN4I GGNNCC 5 cut(s) 67, 139, 140, 513, 556
PspPI GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
PspPPI RGGWCCY 1 cut(s) 138
PstI CTGCAG 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 75, 597
RsaNI GTAC 2 cut(s) 74, 596
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 3 cut(s) 393, 438, 444
Sau96I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
SchI GAGTC 1 cut(s) 368
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 5 cut(s) 66, 138, 210, 247, 317
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 4 cut(s) 22, 221, 413, 572
SmoI CTYRAG 4 cut(s) 22, 221, 413, 572
StyI CCWWGG 2 cut(s) 378, 540
TaaI ACNGT 2 cut(s) 118, 592
TaiI ACGT 1 cut(s) 161
TaqII GACCGA 1 cut(s) 198
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 2 cut(s) 217, 353
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 3 cut(s) 196, 435, 475
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 535
TspRI CASTG 3 cut(s) 196, 435, 475
VpaK11BI GGWCC 5 cut(s) 66, 138, 210, 247, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.