RLG00000002507

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002385
Physical Location & Seq
Reverse (-)
35411144 .. 35412538
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002507

Sequence Viewer

Length: 606 bp
ATGTCATCTCTACAACGCTTCTCAAGCCATGCTCATGTTTCTCAAACCATTACTACTATCTTAGGGTCCAAAGTACATCTTCAAAGCAAGGCTGTAATACACTCACCTACCAGAACTGTGGCTCAACAAATATCAGGGTCTCAATCCAGATCTGCCACGTGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCCTCAGTCCAAGAATCTTGAGCGCATTGCTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAGGCTAAAAATGGGCCTCAACCGATATGGTCCAGCAGTCTCAGGGATAGGCCCGACATCTTCCGAATCTGGGTCTGCCTCCACAGACTCCAAGGTGGTGGAGAAGACCGCATTTGATATAAAGCTTGAGAAGTTTGACGCAGCGGCAAAGATCAAGATAATAAAGGAGGTTAGGAGTTTCACTGATTTGGGACTAAAGGACGCTAAGGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAGAAGGGTGTCACCAAAGAGGAGGCAGGCCCCATTGTTCACAAGCTCAAGGAGTTGGGTGCTACTGTGGTACTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

202

Amino Acids

21.74

Weight (kDa)

9.57

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 134 - 200 4e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 396, 431
AcuI CTGAAG 2 cut(s) 271, 545
AcvI CACGTG 1 cut(s) 159
AfaI GTAC 2 cut(s) 75, 597
AfiI CCNNNNNNNGG 1 cut(s) 357
AgsI TTSAA 1 cut(s) 83
AluBI AGCT 2 cut(s) 412, 571
AluI AGCT 2 cut(s) 412, 571
Alw26I GTCTC 2 cut(s) 144, 331
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 3 cut(s) 301, 337, 553
ApeKI GCWGC 2 cut(s) 194, 428
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 6 cut(s) 66, 247, 301, 317, 338, 554
AsuHPI GGTGA 2 cut(s) 96, 529
AvaII GGWCC 3 cut(s) 66, 247, 317
BbrPI CACGTG 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 206, 440
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 2 cut(s) 144, 331
BfmI CTRYAG 1 cut(s) 192
BglII AGATCT 1 cut(s) 149
BisI GCNGC 3 cut(s) 195, 429, 432
BlsI GCNGC 3 cut(s) 196, 430, 433
Bme18I GGWCC 3 cut(s) 66, 247, 317
BmgT120I GGNCC 6 cut(s) 66, 247, 301, 317, 338, 554
BmiI GGNNCC 3 cut(s) 67, 513, 556
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 4 cut(s) 7, 242, 434, 557
BsaAI YACGTR 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 378
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 2 cut(s) 515, 603
Bse3DI GCAATG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 221, 342
BseNI ACTGG 2 cut(s) 515, 603
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 2 cut(s) 206, 440
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 3 cut(s) 303, 339, 555
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 2 cut(s) 144, 331
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 3 cut(s) 303, 339, 555
Bso31I GGTCTC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 149, 438
BspACI CCGC 2 cut(s) 396, 431
BspANI GGCC 3 cut(s) 303, 339, 555
BspCNI CTCAG 2 cut(s) 220, 341
BspLI GGNNCC 3 cut(s) 67, 513, 556
BspMAI CTGCAG 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 515, 603
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 2 cut(s) 149, 438
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 118, 592
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 4 cut(s) 61, 207, 328, 492
BstF5I GGATG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 152, 441
BstMAI GTCTC 2 cut(s) 144, 331
BstMBI GATC 2 cut(s) 149, 438
BstMWI GCNNNNNNNGC 2 cut(s) 24, 194
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 206, 440
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 2 cut(s) 118, 385
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 3 cut(s) 303, 339, 555
BtsCI GGATG 1 cut(s) 279
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 189, 468
Cac8I GCNNGC 1 cut(s) 553
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 6 cut(s) 66, 247, 301, 317, 338, 554
CseI GACGC 2 cut(s) 434, 497
Csp6I GTAC 2 cut(s) 74, 596
CviAII CATG 3 cut(s) 29, 35, 269
CviQI GTAC 2 cut(s) 74, 596
DdeI CTNAG 4 cut(s) 61, 207, 328, 492
DpnI GATC 2 cut(s) 151, 440
DpnII GATC 2 cut(s) 149, 438
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 144
Eco47I GGWCC 3 cut(s) 66, 247, 317
Eco57I CTGAAG 2 cut(s) 271, 545
Eco72I CACGTG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 554
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 3 cut(s) 32, 38, 272
FaiI YATR 5 cut(s) 30, 36, 270, 315, 407
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 1 cut(s) 492
FatI CATG 3 cut(s) 28, 34, 268
Fnu4HI GCNGC 3 cut(s) 195, 429, 432
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 3 cut(s) 195, 429, 432
GlaI GCGC 1 cut(s) 227
GluI GCNGC 3 cut(s) 195, 429, 432
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 3 cut(s) 303, 339, 555
HgaI GACGC 2 cut(s) 434, 497
HhaI GCGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 32, 38, 272
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 3 cut(s) 217, 353, 374
HphI GGTGA 2 cut(s) 96, 529
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 147, 221, 442
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 2 cut(s) 118, 592
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 162, 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 194
HpyF3I CTNAG 4 cut(s) 61, 207, 328, 492
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 3 cut(s) 32, 38, 272
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 2 cut(s) 149, 438
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 2 cut(s) 206, 440
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 535
MalI GATC 2 cut(s) 151, 440
MboI GATC 2 cut(s) 149, 438
MboII GAAGA 5 cut(s) 71, 277, 339, 403, 538
MflI RGATCY 1 cut(s) 149
MlyI GAGTC 1 cut(s) 368
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 24, 194
NdeII GATC 2 cut(s) 149, 438
NlaIII CATG 3 cut(s) 32, 38, 272
NlaIV GGNNCC 3 cut(s) 67, 513, 556
NmuCI GTSAC 1 cut(s) 535
PfeI GAWTC 2 cut(s) 217, 353
PkrI GCNGC 3 cut(s) 196, 430, 433
PleI GAGTC 1 cut(s) 368
PmaCI CACGTG 1 cut(s) 159
PmlI CACGTG 1 cut(s) 159
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 159
PspCI CACGTG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 67, 513, 556
PspPI GGNCC 6 cut(s) 66, 247, 301, 317, 338, 554
PstI CTGCAG 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 2 cut(s) 75, 597
RsaNI GTAC 2 cut(s) 74, 596
RseI CAYNNNNRTG 1 cut(s) 33
SatI GCNGC 3 cut(s) 195, 429, 432
Sau3AI GATC 2 cut(s) 149, 438
Sau96I GGNCC 6 cut(s) 66, 247, 301, 317, 338, 554
SchI GAGTC 1 cut(s) 368
SetI ASST 8 cut(s) 109, 161, 243, 252, 384, 414, 459, 573
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 3 cut(s) 66, 247, 317
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 4 cut(s) 22, 221, 413, 572
SmoI CTYRAG 4 cut(s) 22, 221, 413, 572
SsiI CCGC 2 cut(s) 396, 431
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 118, 592
TaiI ACGT 1 cut(s) 161
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 2 cut(s) 217, 353
TscAI CASTG 2 cut(s) 196, 475
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 2 cut(s) 194, 428
Tsp45I GTSAC 1 cut(s) 535
TspRI CASTG 2 cut(s) 196, 475
VpaK11BI GGWCC 3 cut(s) 66, 247, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.