RchiOBHm_Chr7g0217101

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002385
Physical Location & Seq
Forward (+)
35009172 .. 35012461
3290 bp
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UTR
Exon/CDS
Intron
PRQ19428

Sequence Viewer

Length: 681 bp
ATGTCATCTCTACGACGCTTCTCAAGCCATGCTTGTGTTTCTCAAACCATTACTACTATCTTAGGGTCCAAAGTACATCTTCAAAGTAAGGCTGTAATACACTCACCTGCCAGAACTGTGGCTCAACAAATATCAGGGTCCCAATCCAGACCTGCCACGTGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAACTGAGCCTCGGTCCAAGAATCTTGAGTGCATTGCTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAGGTTAAAAATGGGCCTCAACCGATATGGTCCAGCAGTCTCAGGGATAGGCCCGACATCCTCCGAATCTGGGTCTGCCTCCACAGACTCCAAGGTGGTGGAGAAGACCGCGTTTGATATAAAGCTTGAGAAGTTTGACGCAGTGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGATTTGGGACTAAAGGACGCTAAAGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAGAAGGGTGTCACCAAAGAGGAGGCAGGCCCCATTGTTGACAAGCTCAAGGAGTTGGGTGCTACTGTGATTTTAGAGAGCTTGTTGAATTGGGGCTTCTGTGTTTTCTTGTTAAATAGGGAATTGGCTGTTGGATTCTACAATACATGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

226

Amino Acids

24.59

Weight (kDa)

9.27

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 134 - 200 3.2e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
Acc36I ACCTGC 2 cut(s) 115, 160
AccII CGCG 1 cut(s) 398
AciI CCGC 1 cut(s) 396
AcuI CTGAAG 2 cut(s) 271, 545
AcvI CACGTG 1 cut(s) 159
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 357
AgsI TTSAA 2 cut(s) 83, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 639, 671
AluBI AGCT 4 cut(s) 412, 571, 606, 678
AluI AGCT 4 cut(s) 412, 571, 606, 678
Alw26I GTCTC 1 cut(s) 331
AlwNI CAGNNNCTG 2 cut(s) 191, 200
AoxI GGCC 3 cut(s) 301, 337, 553
ApeKI GCWGC 2 cut(s) 194, 675
AspS9I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
AsuHPI GGTGA 2 cut(s) 96, 529
AvaII GGWCC 5 cut(s) 66, 138, 210, 247, 317
BbrPI CACGTG 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 206
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 2 cut(s) 115, 160
BisI GCNGC 2 cut(s) 195, 676
BlsI GCNGC 2 cut(s) 196, 677
Bme18I GGWCC 5 cut(s) 66, 138, 210, 247, 317
BmgT120I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
BmiI GGNNCC 5 cut(s) 67, 139, 140, 513, 556
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 4 cut(s) 7, 242, 434, 557
BsaAI YACGTR 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 206, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 515
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 206, 378
BseGI GGATG 2 cut(s) 279, 344
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 192, 342
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 3 cut(s) 303, 339, 555
BslFI GGGAC 2 cut(s) 124, 492
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 124, 492
BsnI GGCC 3 cut(s) 303, 339, 555
Bsp143I GATC 2 cut(s) 438, 444
BspACI CCGC 1 cut(s) 396
BspANI GGCC 3 cut(s) 303, 339, 555
BspCNI CTCAG 2 cut(s) 193, 341
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 5 cut(s) 67, 139, 140, 513, 556
BspMAI CTGCAG 1 cut(s) 196
BspMI ACCTGC 2 cut(s) 115, 160
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 206, 378
BssMI GATC 2 cut(s) 438, 444
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 118, 592
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 3 cut(s) 61, 201, 328
BstF5I GGATG 2 cut(s) 279, 344
BstFNI CGCG 1 cut(s) 398
BstKTI GATC 2 cut(s) 441, 447
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 2 cut(s) 438, 444
BstMWI GCNNNNNNNGC 2 cut(s) 24, 194
BstNSI RCATGY 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 398
BstXI CCANNNNNNTGG 2 cut(s) 118, 385
BsuRI GGCC 3 cut(s) 303, 339, 555
BtsCI GGATG 2 cut(s) 279, 344
BtsI GCAGTG 2 cut(s) 189, 435
BtsIMutI CAGTG 3 cut(s) 189, 435, 468
BveI ACCTGC 2 cut(s) 115, 160
Cac8I GCNNGC 1 cut(s) 553
CaiI CAGNNNCTG 2 cut(s) 191, 200
Cfr13I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
CseI GACGC 3 cut(s) 24, 434, 497
Csp6I GTAC 1 cut(s) 74
CviAII CATG 3 cut(s) 29, 269, 672
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 3 cut(s) 61, 201, 328
DpnI GATC 2 cut(s) 440, 446
DpnII GATC 2 cut(s) 438, 444
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 378
Eco47I GGWCC 5 cut(s) 66, 138, 210, 247, 317
Eco57I CTGAAG 2 cut(s) 271, 545
Eco72I CACGTG 1 cut(s) 159
EcoO109I RGGNCCY 2 cut(s) 138, 554
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 3 cut(s) 32, 272, 675
FaiI YATR 6 cut(s) 30, 270, 315, 407, 449, 673
FalI AAGNNNNNCTT 4 cut(s) 16, 48, 63, 95
FaqI GGGAC 2 cut(s) 124, 492
FatI CATG 3 cut(s) 28, 268, 671
Fnu4HI GCNGC 2 cut(s) 195, 676
FokI GGATG 2 cut(s) 266, 331
Fsp4HI GCNGC 2 cut(s) 195, 676
GluI GCNGC 2 cut(s) 195, 676
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 3 cut(s) 303, 339, 555
HgaI GACGC 3 cut(s) 24, 434, 497
Hin1II CATG 3 cut(s) 32, 272, 675
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 4 cut(s) 217, 353, 374, 660
HphI GGTGA 2 cut(s) 96, 529
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 147, 221, 442
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 2 cut(s) 118, 592
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 4 cut(s) 162, 194, 228, 675
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 194
HpyF3I CTNAG 3 cut(s) 61, 201, 328
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 3 cut(s) 32, 272, 675
KflI GGGWCCC 1 cut(s) 138
Kzo9I GATC 2 cut(s) 438, 444
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 1 cut(s) 206
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 535
MalI GATC 2 cut(s) 440, 446
MboI GATC 2 cut(s) 438, 444
MboII GAAGA 4 cut(s) 71, 277, 403, 538
MluCI AATT 2 cut(s) 613, 647
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 637
MseI TTAA 2 cut(s) 293, 638
MslI CAYNNNNRTG 1 cut(s) 33
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 2 cut(s) 24, 194
NdeII GATC 2 cut(s) 438, 444
NlaIII CATG 3 cut(s) 32, 272, 675
NlaIV GGNNCC 5 cut(s) 67, 139, 140, 513, 556
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 1 cut(s) 675
PaqCI CACCTGC 1 cut(s) 115
PfeI GAWTC 3 cut(s) 217, 353, 660
PkrI GCNGC 2 cut(s) 196, 677
PleI GAGTC 1 cut(s) 368
PmaCI CACGTG 1 cut(s) 159
PmlI CACGTG 1 cut(s) 159
PpsI GAGTC 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
PspCI CACGTG 1 cut(s) 159
PspN4I GGNNCC 5 cut(s) 67, 139, 140, 513, 556
PspPI GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
PspPPI RGGWCCY 1 cut(s) 138
PstI CTGCAG 1 cut(s) 196
PstNI CAGNNNCTG 2 cut(s) 191, 200
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 2 cut(s) 293, 638
SatI GCNGC 2 cut(s) 195, 676
Sau3AI GATC 2 cut(s) 438, 444
Sau96I GGNCC 8 cut(s) 66, 138, 210, 247, 301, 317, 338, 554
SchI GAGTC 1 cut(s) 368
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 5 cut(s) 66, 138, 210, 247, 317
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 4 cut(s) 22, 221, 413, 572
SmoI CTYRAG 4 cut(s) 22, 221, 413, 572
Sse9I AATT 2 cut(s) 613, 647
SsiI CCGC 1 cut(s) 396
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 118, 592
TaiI ACGT 1 cut(s) 161
TaqII GACCGA 1 cut(s) 198
TasI AATT 2 cut(s) 613, 647
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 3 cut(s) 217, 353, 660
Tru1I TTAA 2 cut(s) 293, 638
Tru9I TTAA 2 cut(s) 293, 638
TscAI CASTG 3 cut(s) 196, 435, 475
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 2 cut(s) 194, 675
Tsp45I GTSAC 1 cut(s) 535
TspRI CASTG 3 cut(s) 196, 435, 475
VpaK11BI GGWCC 5 cut(s) 66, 138, 210, 247, 317
XceI RCATGY 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.