Rroxscaffold_3G00242570

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002385
Physical Location & Seq
Reverse (-)
34979384 .. 34983019
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242570.1

Sequence Viewer

Length: 480 bp
ATGTCCCAAAAGTCCCAATCCAGACCTGCCACATGCAGTCTTTCTCGCACCCTCTACACAGCCACTGCAGCAAGTGAGCCTCGGTCCAAGAATCTTGAGCGCATTGCTGACAACCTCTTGGACCTCACAAAGATTGAGAGGCATGACTACTCCATCCTCTTCAGGCTAAAAATGGGCCTCAACCGATATGGTCCAGCAGTCTCAGGGATAGGCCTGACATCTTCCGAATCTGGGTCTGCCCCCACAGACTCCAAGGTGGTGGAGAAGACTGCATTTGATATAAAGCTTGAGAAGTTTGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGAGTTTCACTGATTTGGGACTAAAGGACGCTAAGGACTTGGTGGAAAAGGCTCCAGTTTTGCTGAAGAAGGGTGTCACCAAAGAGGAGGCAGGCCCCATTGTTGACAAGCTCACGGAGTTGGGTGCTACTGTGGTACTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

159

Amino Acids

17.3

Weight (kDa)

9.12

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 92 - 158 1.8e-22 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AciI CCGC 1 cut(s) 305
AcuI CTGAAG 2 cut(s) 145, 419
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 1 cut(s) 231
AluBI AGCT 2 cut(s) 286, 445
AluI AGCT 2 cut(s) 286, 445
Alw26I GTCTC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 3 cut(s) 175, 211, 427
ApeKI GCWGC 2 cut(s) 68, 302
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 5 cut(s) 84, 121, 175, 191, 428
AsuHPI GGTGA 1 cut(s) 403
AvaII GGWCC 3 cut(s) 84, 121, 191
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 80, 314
BccI CCATC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 3 cut(s) 69, 303, 306
BlsI GCNGC 3 cut(s) 70, 304, 307
Bme18I GGWCC 3 cut(s) 84, 121, 191
BmgT120I GGNCC 5 cut(s) 84, 121, 175, 191, 428
BmiI GGNNCC 2 cut(s) 387, 430
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 2 cut(s) 116, 308
BsaBI GATNNNNATC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 80, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 389, 477
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 80, 252
BseGI GGATG 1 cut(s) 153
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 389, 477
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 80, 314
BshFI GGCC 3 cut(s) 177, 213, 429
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 3 cut(s) 177, 213, 429
Bsp143I GATC 2 cut(s) 312, 318
BspACI CCGC 1 cut(s) 305
BspANI GGCC 3 cut(s) 177, 213, 429
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 387, 430
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 2 cut(s) 389, 477
BssECI CCNNGG 2 cut(s) 80, 252
BssMI GATC 2 cut(s) 312, 318
BssT1I CCWWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 202, 366
BstF5I GGATG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 2 cut(s) 315, 321
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 312, 318
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNSI RCATGY 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 80, 314
BstV2I GAAGAC 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 3 cut(s) 177, 213, 429
BtsCI GGATG 1 cut(s) 153
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 342
BveI ACCTGC 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 5 cut(s) 84, 121, 175, 191, 428
CseI GACGC 2 cut(s) 308, 371
Csp6I GTAC 1 cut(s) 470
CviAII CATG 2 cut(s) 33, 143
CviJI RGCY 9 cut(s) 62, 79, 166, 177, 213, 286, 386, 429, 445
CviKI_1 RGCY 9 cut(s) 62, 79, 166, 177, 213, 286, 386, 429, 445
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 2 cut(s) 202, 366
DpnI GATC 2 cut(s) 314, 320
DpnII GATC 2 cut(s) 312, 318
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 213
Eco47I GGWCC 3 cut(s) 84, 121, 191
Eco57I CTGAAG 2 cut(s) 145, 419
EcoO109I RGGNCCY 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 36, 146
FaiI YATR 5 cut(s) 34, 144, 189, 281, 323
FaqI GGGAC 1 cut(s) 366
FatI CATG 2 cut(s) 32, 142
Fnu4HI GCNGC 3 cut(s) 69, 303, 306
FokI GGATG 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 69, 303, 306
GlaI GCGC 1 cut(s) 101
GluI GCNGC 3 cut(s) 69, 303, 306
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 3 cut(s) 177, 213, 429
HgaI GACGC 2 cut(s) 308, 371
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 36, 146
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 91, 227, 248
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 3 cut(s) 21, 95, 316
Hpy8I GTNNAC 1 cut(s) 439
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 3 cut(s) 36, 68, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 2 cut(s) 202, 366
Hsp92II CATG 2 cut(s) 36, 146
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 312, 318
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 2 cut(s) 80, 314
MaeIII GTNAC 1 cut(s) 409
MalI GATC 2 cut(s) 314, 320
MboI GATC 2 cut(s) 312, 318
MboII GAAGA 4 cut(s) 151, 213, 277, 412
MlyI GAGTC 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 2 cut(s) 312, 318
NlaIII CATG 2 cut(s) 36, 146
NlaIV GGNNCC 2 cut(s) 387, 430
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 36
PceI AGGCCT 1 cut(s) 213
PfeI GAWTC 2 cut(s) 91, 227
PkrI GCNGC 3 cut(s) 70, 304, 307
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 387, 430
PspPI GGNCC 5 cut(s) 84, 121, 175, 191, 428
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
SatI GCNGC 3 cut(s) 69, 303, 306
Sau3AI GATC 2 cut(s) 312, 318
Sau96I GGNCC 5 cut(s) 84, 121, 175, 191, 428
SchI GAGTC 1 cut(s) 242
SetI ASST 7 cut(s) 28, 117, 126, 258, 288, 333, 447
SfcI CTRYAG 1 cut(s) 66
SinI GGWCC 3 cut(s) 84, 121, 191
SmlI CTYRAG 2 cut(s) 95, 287
SmoI CTYRAG 2 cut(s) 95, 287
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 1 cut(s) 305
StuI AGGCCT 1 cut(s) 213
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 466
TaqII GACCGA 1 cut(s) 72
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 91, 227
TscAI CASTG 2 cut(s) 70, 349
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 2 cut(s) 68, 302
Tsp45I GTSAC 1 cut(s) 409
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 2 cut(s) 70, 349
VpaK11BI GGWCC 3 cut(s) 84, 121, 191
XceI RCATGY 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.