MD10G1046200.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002385
Physical Location & Seq
Forward (+)
6175448 .. 6179585
4138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1046200.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCGAGCTCTCTGCAACGCTTCTCCAGCCATGTTCGTCTTTCTCGAACCACTTTTGTCTCAGGATCCAAACCTTTCTTGGTGGACGCATTTGTCGAGATCAAACCTGCTGAGCATGTAATGGGCTTACCTGCCACAACTGCAACCCAACATGGACCAGGGTCCTTATCAAGATTTGCCTCCACTCTTTCCCGTGCCCTCAGCACAGCCACTGCAGCAAATGAGACGCAGACCCAGAAGCTTGAGCGCATTGCTGACGAGCTCCTTGACCTCACAAAGCTCGAGAGGCATGACTACGCCATCCTTTTCAGGCTGAAAATGGGCCTCAATCGGTACGGCCCAGCAATCTCGGGTATTGGCTCGGCATCTTCTGAATCTGGGCCTGCTTCGACAGAATCCAAGATTGCTGAGAAGACTGCATTTGATATAAAGATTGAGAAATTTGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACCGACTTGGGACTGAAGGAGGCTAAAGAGTTGGTGGAGAAGGTCCCTGTTGTGGTGAAGAAAGGGTTAACCAAGGAGGAGGCGGGGCCCATTGTCGACAAGCTTAAGGAGTTGGGTGCCACTGTGGTATTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

209

Amino Acids

22.48

Weight (kDa)

9.2

Isoelectric Point (pI)

26.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 141 - 207 1.1e-20 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
Acc36I ACCTGC 2 cut(s) 115, 139
AccB1I GGYRCC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 585
AciI CCGC 2 cut(s) 452, 572
AclWI GGATC 2 cut(s) 60, 73
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 541
AflII CTTAAG 1 cut(s) 593
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 5 cut(s) 9, 241, 262, 280, 592
AluI AGCT 5 cut(s) 9, 241, 262, 280, 592
Alw21I GWGCWC 2 cut(s) 11, 264
Alw26I GTCTC 2 cut(s) 64, 218
AlwI GGATC 2 cut(s) 60, 73
AlwNI CAGNNNCTG 1 cut(s) 212
Ama87I CYCGRG 2 cut(s) 281, 349
AoxI GGCC 4 cut(s) 322, 337, 380, 575
ApaI GGGCCC 1 cut(s) 579
ApeKI GCWGC 2 cut(s) 215, 449
ApoI RAATTY 1 cut(s) 440
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 8 cut(s) 155, 162, 322, 338, 380, 532, 575, 576
AsuHPI GGTGA 2 cut(s) 481, 556
AvaI CYCGRG 2 cut(s) 281, 349
AvaII GGWCC 3 cut(s) 155, 162, 532
BaeGI GKGCMC 2 cut(s) 199, 579
BamHI GGATCC 1 cut(s) 65
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 3 cut(s) 11, 264, 579
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 11, 264
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 227, 461
BccI CCATC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 352
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 64, 218
BfmI CTRYAG 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 593
BfuAI ACCTGC 2 cut(s) 115, 139
BisI GCNGC 3 cut(s) 216, 450, 453
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 3 cut(s) 217, 451, 454
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 3 cut(s) 155, 162, 532
BmeT110I CYCGRG 2 cut(s) 281, 349
BmgT120I GGNCC 8 cut(s) 155, 162, 322, 338, 380, 532, 575, 576
BmiI GGNNCC 6 cut(s) 67, 163, 534, 576, 577, 607
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 374
BoxI GACNNNNGTC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 464
BsaJI CCNNGG 2 cut(s) 158, 561
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 158, 561
BseGI GGATG 1 cut(s) 300
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 4 cut(s) 75, 102, 214, 399
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 2 cut(s) 199, 579
BseXI GCAGC 2 cut(s) 227, 461
BseYI CCCAGC 1 cut(s) 340
BshFI GGCC 4 cut(s) 324, 339, 382, 577
BshNI GGYRCC 1 cut(s) 605
BsiHKAI GWGCWC 2 cut(s) 11, 264
BsiHKCI CYCGRG 2 cut(s) 281, 349
BslFI GGGAC 2 cut(s) 513, 518
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 2 cut(s) 64, 218
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 513, 518
BsnI GGCC 4 cut(s) 324, 339, 382, 577
BsoBI CYCGRG 2 cut(s) 281, 349
Bsp120I GGGCCC 1 cut(s) 575
Bsp1286I GDGCHC 4 cut(s) 11, 199, 264, 579
Bsp143I GATC 4 cut(s) 65, 99, 459, 465
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 2 cut(s) 452, 572
BspANI GGCC 4 cut(s) 324, 339, 382, 577
BspCNI CTCAG 4 cut(s) 74, 103, 213, 400
BspLI GGNNCC 6 cut(s) 67, 163, 534, 576, 577, 607
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 2 cut(s) 115, 139
BspPI GGATC 2 cut(s) 60, 73
BspT107I GGYRCC 1 cut(s) 605
BspTI CTTAAG 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 158, 561
BssMI GATC 4 cut(s) 65, 99, 459, 465
BssT1I CCWWGG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 613
BstAFI CTTAAG 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 7, 384
BstDEI CTNAG 4 cut(s) 61, 111, 200, 408
BstF5I GGATG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 4 cut(s) 68, 102, 462, 468
BstMAI GTCTC 2 cut(s) 64, 218
BstMBI GATC 4 cut(s) 65, 99, 459, 465
BstMWI GCNNNNNNNGC 4 cut(s) 27, 140, 215, 286
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 119
BstPAI GACNNNNGTC 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 2 cut(s) 199, 579
BstV1I GCAGC 2 cut(s) 227, 461
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 4 cut(s) 324, 339, 382, 577
BtsCI GGATG 1 cut(s) 300
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 210, 609
BveI ACCTGC 2 cut(s) 115, 139
Cac8I GCNNGC 2 cut(s) 7, 384
CaiI CAGNNNCTG 1 cut(s) 212
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 8 cut(s) 155, 162, 322, 338, 380, 532, 575, 576
CseI GACGC 3 cut(s) 95, 235, 455
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 32, 116, 152, 290
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 4 cut(s) 61, 111, 200, 408
DpnI GATC 4 cut(s) 67, 101, 461, 467
DpnII GATC 4 cut(s) 65, 99, 459, 465
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Ecl136II GAGCTC 2 cut(s) 9, 262
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 3 cut(s) 11, 264, 579
Eco47I GGWCC 3 cut(s) 155, 162, 532
Eco53kI GAGCTC 2 cut(s) 9, 262
Eco57I CTGAAG 1 cut(s) 524
Eco88I CYCGRG 2 cut(s) 281, 349
EcoICRI GAGCTC 2 cut(s) 9, 262
EcoO109I RGGNCCY 3 cut(s) 162, 532, 575
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 3 cut(s) 11, 264, 579
ErhI CCWWGG 1 cut(s) 561
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 4 cut(s) 35, 119, 155, 293
FaiI YATR 6 cut(s) 33, 117, 153, 291, 428, 470
FaqI GGGAC 2 cut(s) 513, 518
FatI CATG 4 cut(s) 31, 115, 151, 289
FauI CCCGC 1 cut(s) 565
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 3 cut(s) 216, 450, 453
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 3 cut(s) 11, 264, 579
Fsp4HI GCNGC 3 cut(s) 216, 450, 453
GlaI GCGC 1 cut(s) 248
GluI GCNGC 3 cut(s) 216, 450, 453
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 4 cut(s) 324, 339, 382, 577
HgaI GACGC 3 cut(s) 95, 235, 455
HhaI GCGC 1 cut(s) 249
Hin1II CATG 4 cut(s) 35, 119, 155, 293
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 2 cut(s) 558, 586
HindII GTYRAC 2 cut(s) 558, 586
HindIII AAGCTT 2 cut(s) 239, 590
HinfI GANTC 2 cut(s) 374, 395
HpaI GTTAAC 1 cut(s) 558
HphI GGTGA 2 cut(s) 481, 556
Hpy166II GTNNAC 3 cut(s) 85, 558, 586
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 6 cut(s) 45, 63, 97, 171, 283, 463
Hpy8I GTNNAC 3 cut(s) 85, 558, 586
HpyAV CCTTC 2 cut(s) 499, 523
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4V TGCA 4 cut(s) 16, 143, 215, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 140, 215, 286
HpyF3I CTNAG 4 cut(s) 61, 111, 200, 408
Hsp92II CATG 4 cut(s) 35, 119, 155, 293
HspAI GCGC 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 558
Kzo9I GATC 4 cut(s) 65, 99, 459, 465
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 2 cut(s) 227, 461
LweI GCATC 1 cut(s) 374
MalI GATC 4 cut(s) 67, 101, 461, 467
MboI GATC 4 cut(s) 65, 99, 459, 465
MboII GAAGA 3 cut(s) 360, 424, 559
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 4 cut(s) 11, 199, 264, 579
MluCI AATT 1 cut(s) 440
MnlI CCTC 9 cut(s) 190, 209, 279, 281, 335, 469, 502, 559, 562
MseI TTAA 2 cut(s) 557, 594
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 593
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 4 cut(s) 27, 140, 215, 286
NdeII GATC 4 cut(s) 65, 99, 459, 465
NlaIII CATG 4 cut(s) 35, 119, 155, 293
NlaIV GGNNCC 6 cut(s) 67, 163, 534, 576, 577, 607
NmeAIII GCCGAG 1 cut(s) 341
NspI RCATGY 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 43, 93
PfeI GAWTC 2 cut(s) 374, 395
PkrI GCNGC 3 cut(s) 217, 451, 454
PpuMI RGGWCCY 2 cut(s) 162, 532
PshAI GACNNNNGTC 1 cut(s) 160
Psp124BI GAGCTC 2 cut(s) 11, 264
Psp5II RGGWCCY 2 cut(s) 162, 532
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 6 cut(s) 67, 163, 534, 576, 577, 607
PspOMI GGGCCC 1 cut(s) 575
PspPI GGNCC 8 cut(s) 155, 162, 322, 338, 380, 532, 575, 576
PspPPI RGGWCCY 2 cut(s) 162, 532
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 2 cut(s) 11, 264
SalI GTCGAC 1 cut(s) 584
SaqAI TTAA 2 cut(s) 557, 594
SatI GCNGC 3 cut(s) 216, 450, 453
Sau3AI GATC 4 cut(s) 65, 99, 459, 465
Sau96I GGNCC 8 cut(s) 155, 162, 322, 338, 380, 532, 575, 576
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 4 cut(s) 11, 199, 264, 579
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 3 cut(s) 155, 162, 532
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 3 cut(s) 242, 281, 593
SmoI CTYRAG 3 cut(s) 242, 281, 593
Sse9I AATT 1 cut(s) 440
SsiI CCGC 2 cut(s) 452, 572
SstI GAGCTC 2 cut(s) 11, 264
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 5 cut(s) 46, 96, 282, 389, 585
TasI AATT 1 cut(s) 440
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 374, 395
Tru1I TTAA 2 cut(s) 557, 594
Tru9I TTAA 2 cut(s) 557, 594
TscAI CASTG 2 cut(s) 217, 616
TseI GCWGC 2 cut(s) 215, 449
TspRI CASTG 2 cut(s) 217, 616
Vha464I CTTAAG 1 cut(s) 593
VpaK11BI GGWCC 3 cut(s) 155, 162, 532
XapI RAATTY 1 cut(s) 440
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 2 cut(s) 76, 616
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.