FvH4_2g23030

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000723
Physical Location & Seq
Reverse (-)
18899467 .. 18906255
6789 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23030.t4

Sequence Viewer

Length: 2313 bp
ATGGCTAACAAGATCGAAGGGCCGGAAGAAATGTCTCGTGCCACAGATACCATGATCCGCGGCATGGACAGTGAGAGCATCAAGAAATACTTGTTCAAGATAGCCATGAAAAGCGATTGGGAAGGAGTTGTTCAGGCATATAGGCTAAACAAGCAGCTGGCACACAAGGCAAGAATCACCAAGTCAGGTGAGACGGCATTGCACATAGCAGTGTTAGATGGCCAAGAGGATCGTGTTGAAGAACTAGTAAAACTGGTTTCCTTGGAGGAGCTGAAACTCCAAAACCAGCGAGGGAATACTCCTCTCCATTTTGCCGCAGCACAAGGGAATGTGAGAATGTGCAAGTGCATTGCCAAAGTTCATCCTTCTTTGGTTGGTATTCCCAACAAAGATAAAGAGACCCCTCTCTTCTTGGCTGCACTCCATGGTAAAAAAGACGCCTTCTTGTGTCTCCACTACATTCGTACTCCTAACGAGTCTGATCCAAAATACTACAGTTACTGTAGGCGGGAAGATGGGGATACTATCTTGCACTGTGCAATTTTGGGGGACTACTTTGATTTGGCATTTCAGATTTTAGCCTTGTATGAAGATCTAGTCAATTCTGTTAATGAAGATGGGTTTTCCCCTCTCCATGTTCTGGCGGGTATGCCTTCTGCTTTCATGAGTGGTAGCCACCTTAGACCTTGGACCAAAATCATTTACCACTGTATATTGGTCGATGAGCTGAATGTGGAAGAAGCAGATCACTTCCATAAGCGGGTAACTAATACAATATTGAACAAAGAAAAGGCTCCGAAATACCCAGAAAACTACAAAACCTGCATCAGTTTCTTGCTAGTGTTCTGGAAAATGGCTCGAGTCTTTGTAGAAAGAAGGCAAAAAGAATCCACAGATCTAGAGAGCCCTGAAGAAACTACTGAAGGAAATTCAGGTCCTTACAAGTACACCTGCTCTGAAGAAACTGCTGAAGGAAATTCAGGTCCTTACAAGTACACCTTCTCTGAAGAAACTACTGAAGGAAATAATTCAGGTCCTTACAACACATGCTTTAATTTTGTCAAGGTCTTGTCGAAGGCAATGCTGATTGTTCTTGGATTAGGGTCTACGGAAATAAAGAAGATAAGGAACAAGAAAGAAAAGCACAAATGGGCTGTTCAGATCATGGACAAGCTATTAAAAAGTACTATGATGTATGAAAATGAAGACAGTGGTATGAACCCTTACATAATTTCCGATGATGGCGACCGTATCATCCTTGGAAGCATTGAATTAGAACAACCTGCAATGACACTTCCACCTACTGACCAAGACGAAAACAAAGGAAAAGGAGACATTGGAAAAGGGTCATTGTCGCCAGAATCGACAGAGAAGAAAACCCCTGAGATGGAAAAGAAGGAAACACCCATTTTGATTGCTGCGAAGAATGGTGTGACTGAAATGGTGGAGAAGATCCTAGAACTTTTTCCAGTGGCCATCCATGACCTTAACGCACAGAAAAAGAATATAGTTCTGTTGGCTGTGGAGAACAGGCAACCTCATGTGTACCAACTCTTGCTAAAGAAAAAGATCATGAAAGAAAGCGTCTTCTGCCAAGTGGATAAAGATGGCAATAGTGCATTACACCTTGCAGCAAATCTGGGAGAATATAAGCCTTGGCTAATTCCTGGTGCTGCCTTGCAAATGCAATGGGAAATCAAATGGTATGAGTTTGTGAAAAACTCAATGTCGTTCCACTTCTTCATCCGACACAACAAGAATAGCAAAACCGCAAAGGACATCTTCACCGAATCCCACACCCAAATTGTCATAGACGGCGGGAAGTGGCTGACCAAAACCTCACAGTCTTGCTCCGTCGTGGCTGCTCTCGTCGCCACCGTCGCCTTCGCCACCGCCACCACCGTCCCCGGCGGCATCGACGAGAGAAACGGCAGACCCACCCTTGAAAACCAACCGGCTTTCGAGATCTTCGCCATCGCCTCCCTCGTGGCCCTTTGCTTCTCCATCACATCAATGGTGATGTTCCTGGCCATTTTGACGTCACGGTATGAGGAGAGGGACTTCGAGAAGAACCTGCCGAGAAAGCTCTTGTTCGGCCTGACGTCGCTGTTTTTGTCCATCACTTTTATGCTGATATCGTTTTGTGCCGGACACTTCTTCGTGCTCAAAGACAAACTCAAGTACGCGGCGTTTCCGGTGTATGCAATATCGTGTTTGCCGGTGTCGTTTTTCGCTATAGCGCAGTTTCCGTTGTACGTTGATCTGGTGTGGGCTACCTTTAAGAAGGTGCCGCAGCGGAGTTACAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

771

Amino Acids

87.1

Weight (kDa)

7.04

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 82 - 149 7.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 479 - 557 6.8e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 959
AatII GACGTC 2 cut(s) 2042, 2105
Acc36I ACCTGC 4 cut(s) 830, 959, 1291, 2082
AccB1I GGYRCC 1 cut(s) 2287
AccB7I CCANNNNNTGG 1 cut(s) 640
AccI GTMKAC 1 cut(s) 1106
AccII CGCG 2 cut(s) 60, 2186
AclWI GGATC 4 cut(s) 49, 237, 476, 1447
AcoI YGGCCR 3 cut(s) 220, 1473, 2028
AcsI RAATTY 2 cut(s) 928, 976
AcuI CTGAAG 6 cut(s) 930, 942, 978, 990, 1026, 1038
AcyI GRCGYC 3 cut(s) 438, 2039, 2102
AfaI GTAC 7 cut(s) 466, 947, 995, 1186, 1547, 2183, 2255
AfiI CCNNNNNNNGG 4 cut(s) 64, 640, 760, 1387
AgsI TTSAA 5 cut(s) 97, 239, 781, 1271, 1946
AhlI ACTAGT 1 cut(s) 244
AjnI CCWGG 2 cut(s) 1666, 2025
AluBI AGCT 5 cut(s) 157, 271, 727, 1174, 2086
AluI AGCT 5 cut(s) 157, 271, 727, 1174, 2086
Alw21I GWGCWC 1 cut(s) 2166
Alw26I GTCTC 5 cut(s) 39, 185, 392, 455, 1326
AlwI GGATC 4 cut(s) 49, 237, 476, 1447
AlwNI CAGNNNCTG 1 cut(s) 501
Ama87I CYCGRG 1 cut(s) 858
AoxI GGCC 6 cut(s) 20, 220, 1473, 1989, 2028, 2095
ApeKI GCWGC 8 cut(s) 154, 317, 416, 1418, 1631, 1673, 1862, 2293
ApoI RAATTY 2 cut(s) 928, 976
Asp700I GAANNNNTTC 2 cut(s) 1027, 1464
AspLEI GCGC 1 cut(s) 2242
AspS9I GGNCC 6 cut(s) 20, 690, 935, 983, 1034, 1990
AsuC2I CCSGG 1 cut(s) 1908
AsuHPI GGTGA 4 cut(s) 169, 200, 1777, 2029
AvaI CYCGRG 1 cut(s) 858
AvaII GGWCC 4 cut(s) 690, 935, 983, 1034
BalI TGGCCA 3 cut(s) 222, 1475, 2030
BanI GGYRCC 1 cut(s) 2287
BanII GRGCYC 1 cut(s) 908
BauI CACGAG 2 cut(s) 36, 1985
BbsI GAAGAC 2 cut(s) 1212, 1579
Bbv12I GWGCWC 1 cut(s) 2166
BbvI GCAGC 8 cut(s) 166, 329, 403, 1405, 1643, 1660, 1849, 2305
BceAI ACGGC 3 cut(s) 210, 1831, 1945
BcgI CGANNNNNNTGC 2 cut(s) 1063, 1097
BciT130I CCWGG 2 cut(s) 1668, 2027
BciVI GTATCC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 1908
BcoDI GTCTC 5 cut(s) 39, 185, 392, 455, 1326
BcuI ACTAGT 1 cut(s) 244
BfaI CTAG 5 cut(s) 245, 596, 839, 899, 1457
BfmI CTRYAG 3 cut(s) 493, 502, 2235
BfuAI ACCTGC 4 cut(s) 830, 959, 1291, 2082
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 3 cut(s) 592, 895, 1965
BmcAI AGTACT 1 cut(s) 1186
Bme1390I CCNGG 3 cut(s) 1668, 1908, 2027
Bme18I GGWCC 4 cut(s) 690, 935, 983, 1034
BmeT110I CYCGRG 1 cut(s) 858
BmgT120I GGNCC 6 cut(s) 20, 690, 935, 983, 1034, 1990
BmiI GGNNCC 2 cut(s) 795, 2289
BmrFI CCNGG 3 cut(s) 1668, 1908, 2027
BmsI GCATC 3 cut(s) 87, 834, 1923
BpiI GAAGAC 2 cut(s) 1212, 1579
BpuEI CTTGAG 1 cut(s) 2162
BpuMI CCSGG 1 cut(s) 1908
BsaHI GRCGYC 3 cut(s) 438, 2039, 2102
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 7 cut(s) 58, 261, 424, 686, 1258, 1655, 1906
BsaWI WCCGGW 1 cut(s) 2194
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 640, 760, 1387
Bse118I RCCGGY 2 cut(s) 1954, 2218
Bse1I ACTGG 2 cut(s) 258, 1469
Bse3DI GCAATG 5 cut(s) 197, 348, 1086, 1293, 1694
BseBI CCWGG 2 cut(s) 1668, 2027
BseDI CCNNGG 7 cut(s) 58, 261, 424, 686, 1258, 1655, 1906
BseGI GGATG 4 cut(s) 361, 1254, 1476, 1743
BseLI CCNNNNNNNGG 4 cut(s) 64, 640, 760, 1387
BseMI GCAATG 5 cut(s) 197, 348, 1086, 1293, 1694
BseMII CTCAG 1 cut(s) 1374
BseNI ACTGG 2 cut(s) 258, 1469
BseRI GAGGAG 3 cut(s) 281, 291, 2066
BseXI GCAGC 8 cut(s) 166, 329, 403, 1405, 1643, 1660, 1849, 2305
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 2 cut(s) 60, 2186
Bsh1285I CGRYCG 1 cut(s) 1249
BshFI GGCC 6 cut(s) 22, 222, 1475, 1991, 2030, 2097
BshNI GGYRCC 1 cut(s) 2287
BsiEI CGRYCG 1 cut(s) 1249
BsiHKAI GWGCWC 1 cut(s) 2166
BsiHKCI CYCGRG 1 cut(s) 858
BsiSI CCGG 6 cut(s) 23, 1908, 1955, 2148, 2195, 2219
BslFI GGGAC 3 cut(s) 563, 1889, 2072
BslI CCNNNNNNNGG 4 cut(s) 64, 640, 760, 1387
BsmAI GTCTC 5 cut(s) 39, 185, 392, 455, 1326
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 3 cut(s) 563, 1889, 2072
BsnI GGCC 6 cut(s) 22, 222, 1475, 1991, 2030, 2097
Bso31I GGTCTC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 858
Bsp1286I GDGCHC 2 cut(s) 908, 2166
Bsp19I CCATGG 1 cut(s) 424
BspANI GGCC 6 cut(s) 22, 222, 1475, 1991, 2030, 2097
BspCNI CTCAG 1 cut(s) 1375
BspFNI CGCG 2 cut(s) 60, 2186
BspHI TCATGA 2 cut(s) 663, 1572
BspLI GGNNCC 2 cut(s) 795, 2289
BspMI ACCTGC 4 cut(s) 830, 959, 1291, 2082
BspPI GGATC 4 cut(s) 49, 237, 476, 1447
BspT107I GGYRCC 1 cut(s) 2287
BspTNI GGTCTC 1 cut(s) 392
BsrDI GCAATG 5 cut(s) 197, 348, 1086, 1293, 1694
BsrFI RCCGGY 2 cut(s) 1954, 2218
BsrI ACTGG 2 cut(s) 258, 1469
BssAI RCCGGY 2 cut(s) 1954, 2218
BssECI CCNNGG 7 cut(s) 58, 261, 424, 686, 1258, 1655, 1906
BssNI GRCGYC 3 cut(s) 438, 2039, 2102
BssSI CACGAG 2 cut(s) 36, 1985
BssT1I CCWWGG 5 cut(s) 261, 424, 686, 1258, 1655
Bst2BI CACGAG 2 cut(s) 36, 1985
Bst2UI CCWGG 2 cut(s) 1668, 2027
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 3 cut(s) 438, 2039, 2102
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 2 cut(s) 680, 1383
BstDSI CCRYGG 2 cut(s) 58, 424
BstF5I GGATG 4 cut(s) 361, 1254, 1476, 1743
BstFNI CGCG 2 cut(s) 60, 2186
BstHHI GCGC 1 cut(s) 2242
BstMAI GTCTC 5 cut(s) 39, 185, 392, 455, 1326
BstMCI CGRYCG 1 cut(s) 1249
BstMWI GCNNNNNNNGC 6 cut(s) 151, 167, 1590, 1871, 1880, 2083
BstNI CCWGG 2 cut(s) 1668, 2027
BstNSI RCATGY 1 cut(s) 1050
BstSCI CCNGG 3 cut(s) 1666, 1906, 2025
BstSFI CTRYAG 3 cut(s) 493, 502, 2235
BstUI CGCG 2 cut(s) 60, 2186
BstV1I GCAGC 8 cut(s) 166, 329, 403, 1405, 1643, 1660, 1849, 2305
BstV2I GAAGAC 2 cut(s) 1212, 1579
BstX2I RGATCY 4 cut(s) 592, 895, 1452, 1965
BstYI RGATCY 4 cut(s) 592, 895, 1452, 1965
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 6 cut(s) 22, 222, 1475, 1991, 2030, 2097
BtgI CCRYGG 2 cut(s) 58, 424
BtgZI GCGATG 1 cut(s) 1960
BtsCI GGATG 4 cut(s) 361, 1254, 1476, 1743
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 6 cut(s) 76, 216, 532, 706, 1216, 1476
BveI ACCTGC 4 cut(s) 830, 959, 1291, 2082
Cac8I GCNNGC 1 cut(s) 159
CaiI CAGNNNCTG 1 cut(s) 501
CciI TCATGA 2 cut(s) 663, 1572
CfoI GCGC 1 cut(s) 2242
Cfr10I RCCGGY 2 cut(s) 1954, 2218
Cfr13I GGNCC 6 cut(s) 20, 690, 935, 983, 1034, 1990
Cfr42I CCGCGG 1 cut(s) 61
CseI GACGC 2 cut(s) 446, 1573
Csp6I GTAC 7 cut(s) 465, 946, 994, 1185, 1546, 2182, 2254
CviQI GTAC 7 cut(s) 465, 946, 994, 1185, 1546, 2182, 2254
DdeI CTNAG 2 cut(s) 680, 1383
EaeI YGGCCR 3 cut(s) 220, 1473, 2028
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 5 cut(s) 261, 424, 686, 1258, 1655
Eco24I GRGCYC 1 cut(s) 908
Eco31I GGTCTC 1 cut(s) 392
Eco32I GATATC 1 cut(s) 2136
Eco47I GGWCC 4 cut(s) 690, 935, 983, 1034
Eco57I CTGAAG 6 cut(s) 930, 942, 978, 990, 1026, 1038
Eco88I CYCGRG 1 cut(s) 858
EcoO109I RGGNCCY 3 cut(s) 935, 983, 1034
EcoRII CCWGG 2 cut(s) 1666, 2025
EcoRV GATATC 1 cut(s) 2136
EcoT14I CCWWGG 5 cut(s) 261, 424, 686, 1258, 1655
EcoT38I GRGCYC 1 cut(s) 908
ErhI CCWWGG 5 cut(s) 261, 424, 686, 1258, 1655
Esp3I CGTCTC 1 cut(s) 185
FalI AAGNNNNNCTT 6 cut(s) 74, 106, 983, 1015, 1766, 1798
FaqI GGGAC 3 cut(s) 563, 1889, 2072
FauI CCCGC 4 cut(s) 501, 637, 753, 1811
FblI GTMKAC 1 cut(s) 1106
FokI GGATG 4 cut(s) 348, 1241, 1463, 1730
FriOI GRGCYC 1 cut(s) 908
FspBI CTAG 5 cut(s) 245, 596, 839, 899, 1457
GlaI GCGC 1 cut(s) 2241
HaeIII GGCC 6 cut(s) 22, 222, 1475, 1991, 2030, 2097
HapII CCGG 6 cut(s) 23, 1908, 1955, 2148, 2195, 2219
HgaI GACGC 2 cut(s) 446, 1573
HhaI GCGC 1 cut(s) 2242
Hin1I GRCGYC 3 cut(s) 438, 2039, 2102
Hin6I GCGC 1 cut(s) 2240
HinP1I GCGC 1 cut(s) 2240
HinfI GANTC 6 cut(s) 174, 476, 861, 887, 1361, 1790
HpaII CCGG 6 cut(s) 23, 1908, 1955, 2148, 2195, 2219
HphI GGTGA 4 cut(s) 169, 200, 1777, 2029
Hpy166II GTNNAC 4 cut(s) 948, 996, 1107, 1546
Hpy188I TCNGA 8 cut(s) 481, 573, 798, 958, 1006, 1161, 1237, 1748
Hpy188III TCNNGA 8 cut(s) 82, 97, 664, 847, 899, 1573, 1963, 2065
Hpy8I GTNNAC 4 cut(s) 948, 996, 1107, 1546
Hpy99I CGWCG 5 cut(s) 1859, 1874, 1883, 1922, 2107
HpyCH4IV ACGT 3 cut(s) 2039, 2102, 2256
HpyF10VI GCNNNNNNNGC 6 cut(s) 151, 167, 1590, 1871, 1880, 2083
HpyF3I CTNAG 2 cut(s) 680, 1383
HpySE526I ACGT 3 cut(s) 2039, 2102, 2256
Hsp92I GRCGYC 3 cut(s) 438, 2039, 2102
HspAI GCGC 1 cut(s) 2240
KspI CCGCGG 1 cut(s) 61
LmnI GCTCC 3 cut(s) 268, 799, 1856
Lsp1109I GCAGC 8 cut(s) 166, 329, 403, 1405, 1643, 1660, 1849, 2305
LweI GCATC 3 cut(s) 87, 834, 1923
MaeI CTAG 5 cut(s) 245, 596, 839, 899, 1457
MaeII ACGT 3 cut(s) 2039, 2102, 2256
MaeIII GTNAC 5 cut(s) 497, 763, 1432, 2040, 2300
MflI RGATCY 4 cut(s) 592, 895, 1452, 1965
MhlI GDGCHC 2 cut(s) 908, 2166
MlsI TGGCCA 3 cut(s) 222, 1475, 2030
MluNI TGGCCA 3 cut(s) 222, 1475, 2030
MlyI GAGTC 2 cut(s) 485, 870
MmeI TCCRAC 1 cut(s) 1771
Mox20I TGGCCA 3 cut(s) 222, 1475, 2030
MroXI GAANNNNTTC 2 cut(s) 1027, 1464
MscI TGGCCA 3 cut(s) 222, 1475, 2030
MseI TTAA 5 cut(s) 609, 1053, 1178, 1488, 2280
MslI CAYNNNNRTG 3 cut(s) 209, 2012, 2126
Msp20I TGGCCA 3 cut(s) 222, 1475, 2030
MspA1I CMGCKG 3 cut(s) 60, 157, 2296
MspI CCGG 6 cut(s) 23, 1908, 1955, 2148, 2195, 2219
MspR9I CCNGG 3 cut(s) 1668, 1908, 2027
MvaI CCWGG 2 cut(s) 1668, 2027
MvnI CGCG 2 cut(s) 60, 2186
MwoI GCNNNNNNNGC 6 cut(s) 151, 167, 1590, 1871, 1880, 2083
NciI CCSGG 1 cut(s) 1908
NcoI CCATGG 1 cut(s) 424
NlaIV GGNNCC 2 cut(s) 795, 2289
NmeAIII GCCGAG 1 cut(s) 2103
NmuCI GTSAC 2 cut(s) 1432, 2040
NspI RCATGY 1 cut(s) 1050
PaeR7I CTCGAG 1 cut(s) 858
PagI TCATGA 2 cut(s) 663, 1572
PaqCI CACCTGC 1 cut(s) 959
PcsI WCGNNNNNNNCGW 3 cut(s) 1361, 1875, 1983
PdmI GAANNNNTTC 2 cut(s) 1027, 1464
PfeI GAWTC 4 cut(s) 174, 887, 1361, 1790
PflMI CCANNNNNTGG 1 cut(s) 640
PleI GAGTC 2 cut(s) 484, 869
PpsI GAGTC 2 cut(s) 484, 869
PpuMI RGGWCCY 3 cut(s) 935, 983, 1034
Psp5II RGGWCCY 3 cut(s) 935, 983, 1034
Psp6I CCWGG 2 cut(s) 1666, 2025
PspGI CCWGG 2 cut(s) 1666, 2025
PspN4I GGNNCC 2 cut(s) 795, 2289
PspPI GGNCC 6 cut(s) 20, 690, 935, 983, 1034, 1990
PspPPI RGGWCCY 3 cut(s) 935, 983, 1034
PspXI VCTCGAGB 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 501
PsuI RGATCY 4 cut(s) 592, 895, 1452, 1965
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 7 cut(s) 466, 947, 995, 1186, 1547, 2183, 2255
RsaNI GTAC 7 cut(s) 465, 946, 994, 1185, 1546, 2182, 2254
RseI CAYNNNNRTG 3 cut(s) 209, 2012, 2126
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 5 cut(s) 609, 1053, 1178, 1488, 2280
Sau96I GGNCC 6 cut(s) 20, 690, 935, 983, 1034, 1990
ScaI AGTACT 1 cut(s) 1186
SchI GAGTC 2 cut(s) 485, 870
ScrFI CCNGG 3 cut(s) 1668, 1908, 2027
SduI GDGCHC 2 cut(s) 908, 2166
SfaNI GCATC 3 cut(s) 87, 834, 1923
SfcI CTRYAG 3 cut(s) 493, 502, 2235
Sfr274I CTCGAG 1 cut(s) 858
Sfr303I CCGCGG 1 cut(s) 61
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 4 cut(s) 690, 935, 983, 1034
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 3 cut(s) 209, 2012, 2126
SmlI CTYRAG 2 cut(s) 858, 2177
SmoI CTYRAG 2 cut(s) 858, 2177
SpeI ACTAGT 1 cut(s) 244
SspI AATATT 1 cut(s) 777
SspMI CTAG 5 cut(s) 245, 596, 839, 899, 1457
StyD4I CCNGG 3 cut(s) 1666, 1906, 2025
StyI CCWWGG 5 cut(s) 261, 424, 686, 1258, 1655
TaiI ACGT 3 cut(s) 2042, 2105, 2259
TaqI TCGA 8 cut(s) 15, 720, 859, 1073, 1364, 1917, 1962, 2064
TatI WGTACW 3 cut(s) 945, 993, 1184
TauI GCSGC 5 cut(s) 63, 317, 1914, 2189, 2293
TfiI GAWTC 4 cut(s) 174, 887, 1361, 1790
Tru1I TTAA 5 cut(s) 609, 1053, 1178, 1488, 2280
Tru9I TTAA 5 cut(s) 609, 1053, 1178, 1488, 2280
TscAI CASTG 6 cut(s) 76, 216, 539, 713, 1216, 1476
TseFI GTSAC 2 cut(s) 1432, 2040
TseI GCWGC 8 cut(s) 154, 317, 416, 1418, 1631, 1673, 1862, 2293
Tsp45I GTSAC 2 cut(s) 1432, 2040
TspGWI ACGGA 3 cut(s) 1124, 1843, 2238
TspRI CASTG 6 cut(s) 76, 216, 539, 713, 1216, 1476
Van91I CCANNNNNTGG 1 cut(s) 640
VpaK11BI GGWCC 4 cut(s) 690, 935, 983, 1034
XapI RAATTY 2 cut(s) 928, 976
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 1 cut(s) 1050
XcmI CCANNNNNNNNNTGG 1 cut(s) 2011
XhoI CTCGAG 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 1106
XmnI GAANNNNTTC 2 cut(s) 1027, 1464
XspI CTAG 5 cut(s) 245, 596, 839, 899, 1457
ZraI GACGTC 2 cut(s) 2040, 2103
ZrmI AGTACT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.