pycom03g20650

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000723
Physical Location & Seq
Reverse (-)
21132123 .. 21133516
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20650.1

Sequence Viewer

Length: 1014 bp
ATGGCTTCTACCGTGAACTCAAACAAAGAGGCAGGTTCTATCGCAGATGATGGCGAGATCAAGAGTATGGACCCAGAGAAGATTAAGAAATACTTGTTCCGAATTGCCATGCAAAGCAAGTGGGAAGAAGTTATTAGAACCTATAAGCTCAACGAGAAGGCTCGTGGGGCAAGAATCACCAAATCAGGTGACACAGCACTACACATAGCAGTATCCGATGGCCAAGAAGAACATGTTGAAAAGCTAGTAAAACTGATTACTGAAGAGGAGCTTAAAATTAAAAACGAGCGAGGGAATACCCCGCTCCACGTCGCAGCATGGATGGGAAACGAGACAATGTGTTTGTGCATTGCCAATGATCATCCATCATTGGTCGGTGCTCTGAATGTAGACAATGAGACTCCTCTATTCTTGGCTGCTGCCTATGGTAAAAAAAATGCCTTCTTGCGCATGCACTACATCTGTAGCCGCAATGGTCTCCCGAACTACAATTATTGTAGGAGAAATGATGGTGATACCATCTTGCATTGTGCAATTGCCGGGGACTACTTTGATTTGGCATTTCAGATAATTCACTTGTATGAAGATCTTGTTAATTATGTCAATGCGGAAGGCTTTTCCCCTCTCCATCTTCTGGCTACCAAGCCTTCTGCCTTCAAAAGCGGTAGCCACCTCAGCCGATTCCAAAATATTATTTACTACTGTATTTATGTTGATGAGCTCAAGGCGGAGCGATGGGATCCATATAATGAGGGAATAATTAAGACATTCAAAGAGGAAAAGAATCCCAAATATCCAGAAAACTACCATACATGCATCAACTTCATTAGGTTGTTCGGGACACCAATTCTCAGCGTTATTCAAAACCATGCCAATCAGAGAAAAGAAACACAATCAGCAGGTACAGGGAGTGTCCCTCAAGTACAGAAAGGAGAAACAAAATCAGCAGATCCAGAGAACCCTGCAGAACCTAAAAAGCAAACTCTCAACACTTCAAATCCAGGTAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

338

Amino Acids

38.01

Weight (kDa)

6.61

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 59 - 115 5.6e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 449
Acc36I ACCTGC 2 cut(s) 23, 890
AccB7I CCANNNNNTGG 1 cut(s) 634
AccBSI CCGCTC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 390
AciI CCGC 5 cut(s) 302, 469, 608, 663, 728
AclWI GGATC 3 cut(s) 734, 747, 944
AcoI YGGCCR 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 2 cut(s) 904, 924
AfiI CCNNNNNNNGG 1 cut(s) 634
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 239, 658, 772, 863, 996
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 1000
AluBI AGCT 4 cut(s) 148, 244, 271, 721
AluI AGCT 4 cut(s) 148, 244, 271, 721
Alw21I GWGCWC 2 cut(s) 382, 723
Alw26I GTCTC 3 cut(s) 326, 392, 482
AlwI GGATC 3 cut(s) 734, 747, 944
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 314, 416, 419
ArsI GACNNNNNNTTYG 2 cut(s) 325, 357
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 3 cut(s) 169, 200, 524
AvaII GGWCC 1 cut(s) 70
BalI TGGCCA 1 cut(s) 222
BamHI GGATCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 723
BauI CACGAG 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 382, 723
BbvCI CCTCAGC 1 cut(s) 674
BbvI GCAGC 3 cut(s) 326, 403, 406
BccI CCATC 8 cut(s) 44, 212, 316, 373, 503, 527, 636, 729
BciT130I CCWGG 1 cut(s) 1002
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 3 cut(s) 326, 392, 482
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 463, 963
BfuAI ACCTGC 2 cut(s) 23, 890
BfuI GTATCC 1 cut(s) 223
BglII AGATCT 1 cut(s) 586
BisI GCNGC 4 cut(s) 315, 417, 420, 469
BlsI GCNGC 4 cut(s) 316, 418, 421, 470
Bme1390I CCNGG 2 cut(s) 541, 1002
Bme18I GGWCC 1 cut(s) 70
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 72, 741
BmrFI CCNGG 2 cut(s) 541, 1002
BmsI GCATC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 901, 933
Bpu10I CCTNAGC 1 cut(s) 674
BpuEI CTTGAG 2 cut(s) 707, 903
BpuMI CCSGG 1 cut(s) 541
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 540
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse3DI GCAATG 2 cut(s) 348, 478
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 2 cut(s) 327, 361
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 2 cut(s) 348, 478
BseMII CTCAG 2 cut(s) 688, 865
BseRI GAGGAG 2 cut(s) 281, 393
BseXI GCAGC 3 cut(s) 326, 403, 406
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 382, 723
BsiSI CCGG 1 cut(s) 540
BslFI GGGAC 3 cut(s) 557, 853, 899
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 3 cut(s) 326, 392, 482
BsmFI GGGAC 3 cut(s) 557, 853, 899
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 482
Bsp1286I GDGCHC 2 cut(s) 382, 723
Bsp143I GATC 5 cut(s) 57, 358, 586, 739, 949
BspACI CCGC 5 cut(s) 302, 469, 608, 663, 728
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 2 cut(s) 687, 864
BspLI GGNNCC 2 cut(s) 72, 741
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 2 cut(s) 23, 890
BspPI GGATC 3 cut(s) 734, 747, 944
BspTNI GGTCTC 1 cut(s) 482
BsrBI CCGCTC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 348, 478
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 5 cut(s) 57, 358, 586, 739, 949
BssSI CACGAG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 2 cut(s) 13, 704
Bst6I CTCTTC 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 2 cut(s) 674, 851
BstF5I GGATG 2 cut(s) 327, 361
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 5 cut(s) 60, 361, 589, 742, 952
BstMAI GTCTC 3 cut(s) 326, 392, 482
BstMBI GATC 5 cut(s) 57, 358, 586, 739, 949
BstMWI GCNNNNNNNGC 2 cut(s) 167, 675
BstNI CCWGG 1 cut(s) 1002
BstNSI RCATGY 3 cut(s) 236, 454, 816
BstSCI CCNGG 2 cut(s) 539, 1000
BstSFI CTRYAG 2 cut(s) 463, 963
BstV1I GCAGC 3 cut(s) 326, 403, 406
BstX2I RGATCY 3 cut(s) 586, 739, 949
BstYI RGATCY 3 cut(s) 586, 739, 949
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 1 cut(s) 222
BtgZI GCGATG 1 cut(s) 748
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 2 cut(s) 327, 361
BveI ACCTGC 2 cut(s) 23, 890
Cac8I GCNNGC 1 cut(s) 452
CfoI GCGC 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 903, 923
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 6 cut(s) 109, 233, 318, 451, 813, 869
CviQI GTAC 2 cut(s) 903, 923
DdeI CTNAG 2 cut(s) 674, 851
DpnI GATC 5 cut(s) 59, 360, 588, 741, 951
DpnII GATC 5 cut(s) 57, 358, 586, 739, 949
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 743
Ecl136II GAGCTC 1 cut(s) 721
Eco24I GRGCYC 1 cut(s) 723
Eco31I GGTCTC 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 721
Eco57I CTGAAG 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 1000
EcoT22I ATGCAT 1 cut(s) 818
EcoT38I GRGCYC 1 cut(s) 723
FaeI CATG 6 cut(s) 112, 236, 321, 454, 816, 872
FalI AAGNNNNNCTT 4 cut(s) 77, 109, 255, 287
FaqI GGGAC 3 cut(s) 557, 853, 899
FatI CATG 6 cut(s) 108, 232, 317, 450, 812, 868
FauI CCCGC 1 cut(s) 309
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 4 cut(s) 315, 417, 420, 469
FokI GGATG 2 cut(s) 334, 348
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 4 cut(s) 315, 417, 420, 469
FspBI CTAG 1 cut(s) 245
FspI TGCGCA 1 cut(s) 449
GlaI GCGC 1 cut(s) 449
GluI GCNGC 4 cut(s) 315, 417, 420, 469
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 540
HhaI GCGC 1 cut(s) 450
Hin1II CATG 6 cut(s) 112, 236, 321, 454, 816, 872
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HinfI GANTC 4 cut(s) 174, 400, 681, 784
HpaII CCGG 1 cut(s) 540
HphI GGTGA 3 cut(s) 169, 200, 524
Hpy166II GTNNAC 2 cut(s) 16, 391
Hpy188I TCNGA 5 cut(s) 101, 217, 384, 567, 879
Hpy188III TCNNGA 5 cut(s) 61, 481, 797, 838, 953
Hpy8I GTNNAC 2 cut(s) 16, 391
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 5 cut(s) 151, 451, 605, 657, 664
HpyCH4III ACNGT 2 cut(s) 13, 704
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 7 cut(s) 112, 348, 454, 526, 533, 816, 965
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 675
HpyF3I CTNAG 2 cut(s) 674, 851
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 6 cut(s) 112, 236, 321, 454, 816, 872
HspAI GCGC 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 5 cut(s) 57, 358, 586, 739, 949
LmnI GCTCC 3 cut(s) 268, 309, 730
Lsp1109I GCAGC 3 cut(s) 326, 403, 406
LweI GCATC 1 cut(s) 825
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 2 cut(s) 188, 1004
MalI GATC 5 cut(s) 59, 360, 588, 741, 951
MbiI CCGCTC 1 cut(s) 304
MboI GATC 5 cut(s) 57, 358, 586, 739, 949
MboII GAAGA 6 cut(s) 91, 137, 239, 275, 596, 623
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 3 cut(s) 586, 739, 949
MhlI GDGCHC 2 cut(s) 382, 723
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 8 cut(s) 102, 276, 490, 534, 570, 595, 759, 846
MluNI TGGCCA 1 cut(s) 222
MlyI GAGTC 1 cut(s) 394
MnlI CCTC 9 cut(s) 22, 259, 284, 414, 633, 683, 745, 769, 927
Mox20I TGGCCA 1 cut(s) 222
Mph1103I ATGCAT 1 cut(s) 818
MscI TGGCCA 1 cut(s) 222
MseI TTAA 5 cut(s) 84, 273, 279, 594, 762
MslI CAYNNNNRTG 1 cut(s) 579
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 2 cut(s) 541, 1002
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 1002
MwoI GCNNNNNNNGC 2 cut(s) 167, 675
NciI CCSGG 1 cut(s) 541
NdeII GATC 5 cut(s) 57, 358, 586, 739, 949
NlaIII CATG 6 cut(s) 112, 236, 321, 454, 816, 872
NlaIV GGNNCC 2 cut(s) 72, 741
NmuCI GTSAC 1 cut(s) 188
NsbI TGCGCA 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 3 cut(s) 236, 454, 816
PaeI GCATGC 1 cut(s) 454
PciI ACATGT 1 cut(s) 232
PfeI GAWTC 3 cut(s) 174, 681, 784
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 4 cut(s) 316, 418, 421, 470
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 1000
PspGI CCWGG 1 cut(s) 1000
PspN4I GGNNCC 2 cut(s) 72, 741
PspPI GGNCC 1 cut(s) 70
PstI CTGCAG 1 cut(s) 967
PsuI RGATCY 3 cut(s) 586, 739, 949
RsaI GTAC 2 cut(s) 904, 924
RsaNI GTAC 2 cut(s) 903, 923
RseI CAYNNNNRTG 1 cut(s) 579
SacI GAGCTC 1 cut(s) 723
SaqAI TTAA 5 cut(s) 84, 273, 279, 594, 762
SatI GCNGC 4 cut(s) 315, 417, 420, 469
Sau3AI GATC 5 cut(s) 57, 358, 586, 739, 949
Sau96I GGNCC 1 cut(s) 70
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 2 cut(s) 541, 1002
SduI GDGCHC 2 cut(s) 382, 723
SfaNI GCATC 1 cut(s) 825
SfcI CTRYAG 2 cut(s) 463, 963
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 2 cut(s) 722, 918
SmoI CTYRAG 2 cut(s) 722, 918
SphI GCATGC 1 cut(s) 454
Sse9I AATT 8 cut(s) 102, 276, 490, 534, 570, 595, 759, 846
SsiI CCGC 5 cut(s) 302, 469, 608, 663, 728
SspI AATATT 1 cut(s) 691
SspMI CTAG 1 cut(s) 245
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 2 cut(s) 539, 1000
TaaI ACNGT 2 cut(s) 13, 704
TaiI ACGT 1 cut(s) 312
TasI AATT 8 cut(s) 102, 276, 490, 534, 570, 595, 759, 846
TatI WGTACW 1 cut(s) 922
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 3 cut(s) 174, 681, 784
Tru1I TTAA 5 cut(s) 84, 273, 279, 594, 762
Tru9I TTAA 5 cut(s) 84, 273, 279, 594, 762
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 3 cut(s) 314, 416, 419
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 2 cut(s) 597, 814
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 70
XceI RCATGY 3 cut(s) 236, 454, 816
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.