MD11G1279300.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000723
Physical Location & Seq
Reverse (-)
39703329 .. 39706840
3512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1279300.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGACGATCATGAATATGGACATGGAGGTCATGCAGACATATTTGTTCAAAATCACCCTAACAAGCCAATGGAAAGAAGTTGTTAGAACCTATAGGCTTGACCCTCGAGCACACATGGCGAAGATCACGGAGTCAGGTGACACAGCATTACACGTAGCAGTGTCCGATGGCCAAGAAGAGCACATTGAAGAGCTAGTAAAACTGGTTTCCACAAACGAGCTGCTTCAAGTTCCAAATGAGCGAGGGAATACCCCTCTCCACATTGCAGCATCGATGGGGAATGTGAGAATGTCTGAGTGCATTGCCAGACACCATCCCATGTTGGTTGGTGCTCTTAACAAAGAAAATGAGACCCCTCTCTTCTCGGCTGCTCTCTATGGTAAAAAATCTGCCTTCTTATGTCTACACTACATCTGCAGCCTTCATGATAATCAGCAACGCTACAAGTACTGCAGGAGGAAGGATGGTAATACTATCTTGCATTGTGCAATTGCTAGGGACTACTTTGATTTGGCCTTTCAGATAATCGATCTGTATGAAGAACTAGTTTTTTACGTCAATGAAGAAGGACACTCCCCTCTTCATCTTCTTGCCAGTAAGTCTTATGCATTTGCAAGTGGTAGTCGGCTTGGACCATGGGACAAAATCATTTACAATTATGTATATGTTGGTAAGCTCAAAAGGGAGGAACGACCGGATCATTTTAACGATGAGCTAACGAAGACATTGAAAGATGGAAGCAATCCCAAATATCCAGAAAACTATCAAACATGCATCACCTTCTTTCGGTTGGTGTGGACTATGGTTCAAGTACTTGTTACCAATAGGAAAGACGATGATCAGGGAGAAAGCCGAAAAAATTCAGCAGATACAGAGAACCCAGTAGTTCGACCTACGGTTGCTCTGAACAGAAGAAATCCAGGATCCCAATCAAATATTGGAGCACAGCAACATCAATCAATTCCGAGCCATCACAGAACCTGCTTTGAGTTCGTAAAACTTTTCTCAAAGGCGACATTGCTGATTGCTTTTGGATGGGGGTCTAAGTATATAAAGAGGATAAGGGAAAAGAAACAAAAGCACAAATGGTCAGTTCAAATCATGAACGAGCTACTCAAACGTGCTTCCTTGTATGCGTATGAAGACAACGGTATGAATCCACAGACTGCAGCACCATCCCACAAAAACAAAAAATATGATGAAACAAGGCCTTATGAAGTTGATCATGGCGATGAAGTTGTCACCTTCGGAGTCGGAAGCAATACTAAACAGCCTGTTGTGAATCCTCCGCGACAAGATGAAAGTAAGGCAAAGAATGATAAAAAGAAAATTGCCGAAGAAATGAGAGAGAAGATAACCCTTCATATGGGAAAGAGGGAAACACCCTTCTTGATTGCTGCAAGAAATGGTGTGACTGAAATGGTGGAGAAGATACTTGAACTTTTTCCGGTGGCCATCCGTGACATTAACTCAGAGAGAAAGAATGTAGTGCAGGTGGCTGTGGAGAGCAGGCAACTCCATGTGTACCGGCTCTTGCTAAGGAAAAATATCTCGATGAGAGATAACGTTTTCAGCAAAGTGGATGATAAAGGGAATAGTGTGTTACATCTCGCAGCAATGTTGGGAGACCATCAGCCTTGGCTAAATCATGGGCCTGCGTTCCAAATGCAATGGGAAATCAAATGGTATCAGATCGTAAAGACCTCCATGCCACCACACTTCTTTGTTCGCTTCAATAACGACAACAAGACTCCGAAGGACATCTTCAAAGAAACCCACAAAGGGCTCGTGAAAGACGGAAGGGAATGGCTCACCAAGGCCTCTGAGTCCTGCTCCGTAATGGGGGCCCTCATCGCCACTGTCGCCTTTGCAACTGCAACAACTGTTCCTGGTGGCATCAAGGAGGTTACAGGAAGACCAACCCTCGAAAACTTACCTGTTTTCGACATTTTCGCCATCTCATCGCTCTTTGCCCTATGCTCCTCGGTTACGTCCATGGTCATATTCCTGTCCATCCTCATGTCCCGGTACCAAGACAAGGACTTCAGGAAAGAGCTGCCGAGTAAGCTTTTGCTTGGATTGACATTGCTCTTCGTGTCAATGGTTTCCATGTTGATTTCATTTTGTGCAGGGCATTTCTTTATGCTTAAAGATAAGCTCAAACACGCTGCATTTCCGGTTTATGCAATAACGTGCATGCCTTTAGCAATTTTTGCTGTAGGCCACTTCCCACTGTATTTTAACATGATATTGGCAAACTTTAAAAAGGTTCCATTCGACAGCGGAGTCACAAGGGTAGCTCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

770

Amino Acids

87.84

Weight (kDa)

9.15

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 43 - 97 2.3e-07 Ankyrin repeats (3 copies)
PGG PF13962 602 - 714 2.6e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1483
AasI GACNNNNNNGTC 2 cut(s) 26, 2285
Acc36I ACCTGC 2 cut(s) 989, 1483
Acc65I GGTACC 1 cut(s) 2028
AccB1I GGYRCC 1 cut(s) 2028
AccI GTMKAC 1 cut(s) 403
AccII CGCG 1 cut(s) 1291
AciI CCGC 2 cut(s) 1289, 2283
AclI AACGTT 1 cut(s) 1566
AclWI GGATC 3 cut(s) 705, 918, 931
AcoI YGGCCR 2 cut(s) 169, 1452
AcsI RAATTY 1 cut(s) 859
AcuI CTGAAG 1 cut(s) 2029
AfaI GTAC 4 cut(s) 449, 813, 1526, 2030
AfiI CCNNNNNNNGG 5 cut(s) 786, 1366, 1782, 1842, 2038
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 9 cut(s) 49, 188, 227, 730, 809, 1097, 1439, 1735, 1768
AhlI ACTAGT 1 cut(s) 544
AjnI CCWGG 2 cut(s) 919, 1888
AluBI AGCT 9 cut(s) 193, 220, 676, 715, 1111, 2056, 2068, 2158, 2300
AluI AGCT 9 cut(s) 193, 220, 676, 715, 1111, 2056, 2068, 2158, 2300
Alw21I GWGCWC 4 cut(s) 112, 183, 334, 946
Alw26I GTCTC 2 cut(s) 344, 1620
AlwI GGATC 3 cut(s) 705, 918, 931
AlwNI CAGNNNCTG 1 cut(s) 981
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 8 cut(s) 169, 513, 1208, 1452, 1652, 1818, 1845, 2221
ApaI GGGCCC 1 cut(s) 1849
ApeKI GCWGC 9 cut(s) 220, 266, 368, 417, 1169, 1397, 1613, 2056, 2168
ApoI RAATTY 1 cut(s) 859
Asp700I GAANNNNTTC 2 cut(s) 859, 1443
Asp718I GGTACC 1 cut(s) 2028
AspS9I GGNCC 4 cut(s) 632, 1652, 1845, 1846
AsuC2I CCSGG 1 cut(s) 2026
AsuHPI GGTGA 5 cut(s) 46, 149, 769, 1234, 1804
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 632
BaeGI GKGCMC 1 cut(s) 1849
BalI TGGCCA 2 cut(s) 171, 1454
BamHI GGATCC 1 cut(s) 923
BanI GGYRCC 1 cut(s) 2028
BanII GRGCYC 2 cut(s) 1788, 1849
BauI CACGAG 1 cut(s) 1787
BbsI GAAGAC 3 cut(s) 728, 1149, 1921
Bbv12I GWGCWC 4 cut(s) 112, 183, 334, 946
BbvI GCAGC 9 cut(s) 207, 278, 355, 429, 1181, 1384, 1625, 2043, 2155
BciT130I CCWGG 2 cut(s) 921, 1890
BclI TGATCA 2 cut(s) 838, 1222
BcnI CCSGG 1 cut(s) 2026
BcoDI GTCTC 2 cut(s) 344, 1620
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 3 cut(s) 194, 495, 545
BfmI CTRYAG 5 cut(s) 91, 415, 451, 1167, 2217
BfuAI ACCTGC 2 cut(s) 989, 1483
BisI GCNGC 9 cut(s) 221, 267, 369, 418, 1170, 1398, 1614, 2057, 2169
BlsI GCNGC 9 cut(s) 222, 268, 370, 419, 1171, 1399, 1615, 2058, 2170
BmcAI AGTACT 2 cut(s) 449, 813
Bme1390I CCNGG 3 cut(s) 921, 1890, 2026
Bme18I GGWCC 1 cut(s) 632
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 4 cut(s) 632, 1652, 1845, 1846
BmiI GGNNCC 5 cut(s) 925, 1846, 1847, 2030, 2271
BmrFI CCNGG 3 cut(s) 921, 1890, 2026
BmrI ACTGGG 1 cut(s) 875
BmsI GCATC 3 cut(s) 278, 783, 1905
BmuI ACTGGG 1 cut(s) 875
BpiI GAAGAC 3 cut(s) 728, 1149, 1921
BplI GAGNNNNNCTC 2 cut(s) 1817, 1849
Bpu10I CCTNAGC 1 cut(s) 1538
BpuMI CCSGG 1 cut(s) 2026
Bsa29I ATCGAT 2 cut(s) 272, 528
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 928
BsaI GGTCTC 2 cut(s) 344, 1620
BsaJI CCNNGG 5 cut(s) 635, 1637, 1815, 1983, 1995
BsaWI WCCGGW 3 cut(s) 694, 1447, 2176
Bsc4I CCNNNNNNNGG 5 cut(s) 786, 1366, 1782, 1842, 2038
Bse118I RCCGGY 1 cut(s) 1527
Bse1I ACTGG 3 cut(s) 207, 594, 881
Bse3DI GCAATG 6 cut(s) 261, 300, 1016, 1623, 1676, 2084
Bse8I GATNNNNATC 1 cut(s) 928
BseBI CCWGG 2 cut(s) 921, 1890
BseCI ATCGAT 2 cut(s) 272, 528
BseDI CCNNGG 5 cut(s) 635, 1637, 1815, 1983, 1995
BseGI GGATG 7 cut(s) 313, 469, 1040, 1175, 1455, 1588, 2013
BseJI GATNNNNATC 1 cut(s) 928
BseLI CCNNNNNNNGG 5 cut(s) 786, 1366, 1782, 1842, 2038
BseMI GCAATG 6 cut(s) 261, 300, 1016, 1623, 1676, 2084
BseMII CTCAG 3 cut(s) 285, 1485, 1815
BseNI ACTGG 3 cut(s) 207, 594, 881
BseRI GAGGAG 2 cut(s) 1972, 2292
BseSI GKGCMC 1 cut(s) 1849
BseXI GCAGC 9 cut(s) 207, 278, 355, 429, 1181, 1384, 1625, 2043, 2155
BsgI GTGCAG 2 cut(s) 1511, 2148
Bsh1236I CGCG 1 cut(s) 1291
Bsh1285I CGRYCG 1 cut(s) 695
BshFI GGCC 8 cut(s) 171, 515, 1210, 1454, 1654, 1820, 1847, 2223
BshNI GGYRCC 1 cut(s) 2028
BshVI ATCGAT 2 cut(s) 272, 528
BsiEI CGRYCG 1 cut(s) 695
BsiHKAI GWGCWC 4 cut(s) 112, 183, 334, 946
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 5 cut(s) 695, 1448, 1528, 2026, 2177
BslFI GGGAC 3 cut(s) 512, 653, 2008
BslI CCNNNNNNNGG 5 cut(s) 786, 1366, 1782, 1842, 2038
BsmAI GTCTC 2 cut(s) 344, 1620
BsmFI GGGAC 3 cut(s) 512, 653, 2008
BsnI GGCC 8 cut(s) 171, 515, 1210, 1454, 1654, 1820, 1847, 2223
Bso31I GGTCTC 2 cut(s) 344, 1620
BsoBI CYCGRG 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 1845
Bsp1286I GDGCHC 6 cut(s) 112, 183, 334, 946, 1788, 1849
Bsp143I GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
Bsp19I CCATGG 2 cut(s) 635, 1995
BspACI CCGC 2 cut(s) 1289, 2283
BspANI GGCC 8 cut(s) 171, 515, 1210, 1454, 1654, 1820, 1847, 2223
BspCNI CTCAG 3 cut(s) 286, 1484, 1816
BspDI ATCGAT 2 cut(s) 272, 528
BspFNI CGCG 1 cut(s) 1291
BspHI TCATGA 3 cut(s) 9, 424, 1101
BspLI GGNNCC 5 cut(s) 925, 1846, 1847, 2030, 2271
BspMAI CTGCAG 3 cut(s) 419, 455, 1171
BspMI ACCTGC 2 cut(s) 989, 1483
BspPI GGATC 3 cut(s) 705, 918, 931
BspQI GCTCTTC 3 cut(s) 171, 183, 2096
BspT107I GGYRCC 1 cut(s) 2028
BspTNI GGTCTC 2 cut(s) 344, 1620
BsrDI GCAATG 6 cut(s) 261, 300, 1016, 1623, 1676, 2084
BsrFI RCCGGY 1 cut(s) 1527
BsrI ACTGG 3 cut(s) 207, 594, 881
BssAI RCCGGY 1 cut(s) 1527
BssECI CCNNGG 5 cut(s) 635, 1637, 1815, 1983, 1995
BssMI GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
BssSI CACGAG 1 cut(s) 1787
BssT1I CCWWGG 4 cut(s) 635, 1637, 1815, 1995
Bst2BI CACGAG 1 cut(s) 1787
Bst2UI CCWGG 2 cut(s) 921, 1890
Bst4CI ACNGT 5 cut(s) 898, 1151, 1861, 1885, 2235
Bst6I CTCTTC 5 cut(s) 171, 183, 365, 585, 2096
BstAPI GCANNNNNTGC 1 cut(s) 2213
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 1511, 1656, 2198
BstDEI CTNAG 5 cut(s) 294, 1044, 1471, 1538, 1824
BstDSI CCRYGG 2 cut(s) 635, 1995
BstF5I GGATG 7 cut(s) 313, 469, 1040, 1175, 1455, 1588, 2013
BstFNI CGCG 1 cut(s) 1291
BstKTI GATC 8 cut(s) 9, 126, 532, 700, 841, 926, 1225, 1695
BstMAI GTCTC 2 cut(s) 344, 1620
BstMBI GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
BstMCI CGRYCG 1 cut(s) 695
BstMWI GCNNNNNNNGC 5 cut(s) 116, 1853, 1862, 2065, 2213
BstNI CCWGG 2 cut(s) 921, 1890
BstNSI RCATGY 2 cut(s) 774, 2200
BstSCI CCNGG 3 cut(s) 919, 1888, 2024
BstSFI CTRYAG 5 cut(s) 91, 415, 451, 1167, 2217
BstSLI GKGCMC 1 cut(s) 1849
BstUI CGCG 1 cut(s) 1291
BstV1I GCAGC 9 cut(s) 207, 278, 355, 429, 1181, 1384, 1625, 2043, 2155
BstV2I GAAGAC 3 cut(s) 728, 1149, 1921
BstX2I RGATCY 1 cut(s) 923
BstYI RGATCY 1 cut(s) 923
Bsu15I ATCGAT 2 cut(s) 272, 528
BsuRI GGCC 8 cut(s) 171, 515, 1210, 1454, 1654, 1820, 1847, 2223
BsuTUI ATCGAT 2 cut(s) 272, 528
BtgI CCRYGG 2 cut(s) 635, 1995
BtgZI GCGATG 3 cut(s) 1245, 1837, 1947
BtsCI GGATG 7 cut(s) 313, 469, 1040, 1175, 1455, 1588, 2013
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 3 cut(s) 165, 1857, 2231
BveI ACCTGC 2 cut(s) 989, 1483
Cac8I GCNNGC 3 cut(s) 1511, 1656, 2198
CaiI CAGNNNCTG 1 cut(s) 981
CciI TCATGA 3 cut(s) 9, 424, 1101
Cfr10I RCCGGY 1 cut(s) 1527
Cfr13I GGNCC 4 cut(s) 632, 1652, 1845, 1846
ClaI ATCGAT 2 cut(s) 272, 528
Csp6I GTAC 4 cut(s) 448, 812, 1525, 2029
CviQI GTAC 4 cut(s) 448, 812, 1525, 2029
DdeI CTNAG 5 cut(s) 294, 1044, 1471, 1538, 1824
DpnI GATC 8 cut(s) 8, 125, 531, 699, 840, 925, 1224, 1694
DpnII GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
DraI TTTAAA 1 cut(s) 2263
DrdI GACNNNNNNGTC 2 cut(s) 26, 2285
DseDI GACNNNNNNGTC 2 cut(s) 26, 2285
EaeI YGGCCR 2 cut(s) 169, 1452
Eam1104I CTCTTC 5 cut(s) 171, 183, 365, 585, 2096
EarI CTCTTC 5 cut(s) 171, 183, 365, 585, 2096
Eco130I CCWWGG 4 cut(s) 635, 1637, 1815, 1995
Eco147I AGGCCT 2 cut(s) 1210, 1820
Eco24I GRGCYC 2 cut(s) 1788, 1849
Eco31I GGTCTC 2 cut(s) 344, 1620
Eco47I GGWCC 1 cut(s) 632
Eco57I CTGAAG 1 cut(s) 2029
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 2 cut(s) 1845, 1846
EcoRII CCWGG 2 cut(s) 919, 1888
EcoT14I CCWWGG 4 cut(s) 635, 1637, 1815, 1995
EcoT22I ATGCAT 2 cut(s) 610, 776
EcoT38I GRGCYC 2 cut(s) 1788, 1849
ErhI CCWWGG 4 cut(s) 635, 1637, 1815, 1995
FalI AAGNNNNNCTT 2 cut(s) 1748, 1780
FaqI GGGAC 3 cut(s) 512, 653, 2008
FauNDI CATATG 1 cut(s) 1365
FbaI TGATCA 2 cut(s) 838, 1222
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 9 cut(s) 221, 267, 369, 418, 1170, 1398, 1614, 2057, 2169
FokI GGATG 7 cut(s) 300, 476, 1047, 1162, 1442, 1595, 2000
FriOI GRGCYC 2 cut(s) 1788, 1849
Fsp4HI GCNGC 9 cut(s) 221, 267, 369, 418, 1170, 1398, 1614, 2057, 2169
FspBI CTAG 3 cut(s) 194, 495, 545
GluI GCNGC 9 cut(s) 221, 267, 369, 418, 1170, 1398, 1614, 2057, 2169
HaeIII GGCC 8 cut(s) 171, 515, 1210, 1454, 1654, 1820, 1847, 2223
HapII CCGG 5 cut(s) 695, 1448, 1528, 2026, 2177
HindIII AAGCTT 1 cut(s) 2066
HinfI GANTC 7 cut(s) 131, 1156, 1251, 1282, 1750, 1826, 2286
HpaII CCGG 5 cut(s) 695, 1448, 1528, 2026, 2177
HphI GGTGA 5 cut(s) 46, 149, 769, 1234, 1804
Hpy166II GTNNAC 3 cut(s) 404, 798, 1525
Hpy188III TCNNGA 8 cut(s) 10, 425, 755, 1102, 1390, 1552, 1789, 2047
Hpy8I GTNNAC 3 cut(s) 404, 798, 1525
HpyCH4III ACNGT 5 cut(s) 898, 1151, 1861, 1885, 2235
HpyCH4IV ACGT 6 cut(s) 153, 555, 1120, 1566, 1991, 2192
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 1853, 1862, 2065, 2213
HpyF3I CTNAG 5 cut(s) 294, 1044, 1471, 1538, 1824
HpySE526I ACGT 6 cut(s) 153, 555, 1120, 1566, 1991, 2192
KpnI GGTACC 1 cut(s) 2032
Ksp22I TGATCA 2 cut(s) 838, 1222
Kzo9I GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
LguI GCTCTTC 3 cut(s) 171, 183, 2096
LmnI GCTCC 4 cut(s) 941, 1838, 1985, 2305
Lsp1109I GCAGC 9 cut(s) 207, 278, 355, 429, 1181, 1384, 1625, 2043, 2155
LweI GCATC 3 cut(s) 278, 783, 1905
MaeI CTAG 3 cut(s) 194, 495, 545
MaeII ACGT 6 cut(s) 153, 555, 1120, 1566, 1991, 2192
MaeIII GTNAC 9 cut(s) 137, 817, 1240, 1411, 1460, 1602, 1906, 1987, 2287
MalI GATC 8 cut(s) 8, 125, 531, 699, 840, 925, 1224, 1694
MboI GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 923
MhlI GDGCHC 6 cut(s) 112, 183, 334, 946, 1788, 1849
MlsI TGGCCA 2 cut(s) 171, 1454
MluCI AATT 6 cut(s) 489, 655, 859, 960, 1329, 2208
MluNI TGGCCA 2 cut(s) 171, 1454
MlyI GAGTC 5 cut(s) 140, 1260, 1744, 1835, 2295
MmeI TCCRAC 1 cut(s) 1234
Mox20I TGGCCA 2 cut(s) 171, 1454
Mph1103I ATGCAT 2 cut(s) 610, 776
MroXI GAANNNNTTC 2 cut(s) 859, 1443
MscI TGGCCA 2 cut(s) 171, 1454
MseI TTAA 6 cut(s) 336, 705, 1467, 2148, 2241, 2262
MslI CAYNNNNRTG 3 cut(s) 14, 1230, 2018
Msp20I TGGCCA 2 cut(s) 171, 1454
MspA1I CMGCKG 1 cut(s) 2283
MspI CCGG 5 cut(s) 695, 1448, 1528, 2026, 2177
MspR9I CCNGG 3 cut(s) 921, 1890, 2026
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 2 cut(s) 921, 1890
MvnI CGCG 1 cut(s) 1291
MwoI GCNNNNNNNGC 5 cut(s) 116, 1853, 1862, 2065, 2213
NciI CCSGG 1 cut(s) 2026
NcoI CCATGG 2 cut(s) 635, 1995
NdeI CATATG 1 cut(s) 1365
NdeII GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
NlaIV GGNNCC 5 cut(s) 925, 1846, 1847, 2030, 2271
NmeAIII GCCGAG 2 cut(s) 344, 2085
NmuCI GTSAC 5 cut(s) 137, 1240, 1411, 1460, 2287
NsiI ATGCAT 2 cut(s) 610, 776
NspI RCATGY 2 cut(s) 774, 2200
PaeI GCATGC 1 cut(s) 2200
PaeR7I CTCGAG 1 cut(s) 105
PagI TCATGA 3 cut(s) 9, 424, 1101
PaqCI CACCTGC 1 cut(s) 1483
PceI AGGCCT 2 cut(s) 1210, 1820
PciSI GCTCTTC 3 cut(s) 171, 183, 2096
PdmI GAANNNNTTC 2 cut(s) 859, 1443
PfeI GAWTC 2 cut(s) 1156, 1282
PfoI TCCNGGA 1 cut(s) 919
PkrI GCNGC 9 cut(s) 222, 268, 370, 419, 1171, 1399, 1615, 2058, 2170
PleI GAGTC 5 cut(s) 139, 1259, 1744, 1834, 2294
PpsI GAGTC 5 cut(s) 139, 1259, 1744, 1834, 2294
Ppu21I YACGTR 1 cut(s) 154
Psp1406I AACGTT 1 cut(s) 1566
Psp6I CCWGG 2 cut(s) 919, 1888
PspGI CCWGG 2 cut(s) 919, 1888
PspN4I GGNNCC 5 cut(s) 925, 1846, 1847, 2030, 2271
PspOMI GGGCCC 1 cut(s) 1845
PspPI GGNCC 4 cut(s) 632, 1652, 1845, 1846
PspXI VCTCGAGB 1 cut(s) 105
PstI CTGCAG 3 cut(s) 419, 455, 1171
PstNI CAGNNNCTG 1 cut(s) 981
PsuI RGATCY 1 cut(s) 923
RsaI GTAC 4 cut(s) 449, 813, 1526, 2030
RsaNI GTAC 4 cut(s) 448, 812, 1525, 2029
RseI CAYNNNNRTG 3 cut(s) 14, 1230, 2018
SapI GCTCTTC 3 cut(s) 171, 183, 2096
SaqAI TTAA 6 cut(s) 336, 705, 1467, 2148, 2241, 2262
SatI GCNGC 9 cut(s) 221, 267, 369, 418, 1170, 1398, 1614, 2057, 2169
Sau3AI GATC 8 cut(s) 6, 123, 529, 697, 838, 923, 1222, 1692
Sau96I GGNCC 4 cut(s) 632, 1652, 1845, 1846
ScaI AGTACT 2 cut(s) 449, 813
SchI GAGTC 5 cut(s) 140, 1260, 1744, 1835, 2295
ScrFI CCNGG 3 cut(s) 921, 1890, 2026
SduI GDGCHC 6 cut(s) 112, 183, 334, 946, 1788, 1849
SfaNI GCATC 3 cut(s) 278, 783, 1905
SfcI CTRYAG 5 cut(s) 91, 415, 451, 1167, 2217
Sfr274I CTCGAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 632
SlaI CTCGAG 1 cut(s) 105
SmiMI CAYNNNNRTG 3 cut(s) 14, 1230, 2018
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
SpeI ACTAGT 1 cut(s) 544
SphI GCATGC 1 cut(s) 2200
Sse9I AATT 6 cut(s) 489, 655, 859, 960, 1329, 2208
SseBI AGGCCT 2 cut(s) 1210, 1820
SsiI CCGC 2 cut(s) 1289, 2283
SspI AATATT 1 cut(s) 937
SspMI CTAG 3 cut(s) 194, 495, 545
StuI AGGCCT 2 cut(s) 1210, 1820
StyD4I CCNGG 3 cut(s) 919, 1888, 2024
StyI CCWWGG 4 cut(s) 635, 1637, 1815, 1995
TaaI ACNGT 5 cut(s) 898, 1151, 1861, 1885, 2235
TaiI ACGT 6 cut(s) 156, 558, 1123, 1569, 1994, 2195
TaqI TCGA 8 cut(s) 106, 272, 528, 889, 1553, 1926, 1944, 2277
TasI AATT 6 cut(s) 489, 655, 859, 960, 1329, 2208
TatI WGTACW 2 cut(s) 447, 811
TfiI GAWTC 2 cut(s) 1156, 1282
Tru1I TTAA 6 cut(s) 336, 705, 1467, 2148, 2241, 2262
Tru9I TTAA 6 cut(s) 336, 705, 1467, 2148, 2241, 2262
TscAI CASTG 3 cut(s) 165, 1864, 2238
TseFI GTSAC 5 cut(s) 137, 1240, 1411, 1460, 2287
TseI GCWGC 9 cut(s) 220, 266, 368, 417, 1169, 1397, 1613, 2056, 2168
Tsp45I GTSAC 5 cut(s) 137, 1240, 1411, 1460, 2287
TspGWI ACGGA 4 cut(s) 143, 1448, 1812, 1825
TspRI CASTG 3 cut(s) 165, 1864, 2238
VpaK11BI GGWCC 1 cut(s) 632
XapI RAATTY 1 cut(s) 859
XceI RCATGY 2 cut(s) 774, 2200
XcmI CCANNNNNNNNNTGG 1 cut(s) 935
XhoI CTCGAG 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 403
XmnI GAANNNNTTC 2 cut(s) 859, 1443
XspI CTAG 3 cut(s) 194, 495, 545
ZrmI AGTACT 2 cut(s) 449, 813
Zsp2I ATGCAT 2 cut(s) 610, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.