pycom03g20620

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000723
Physical Location & Seq
Reverse (-)
21101764 .. 21103217
1454 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20620.2

Sequence Viewer

Length: 936 bp
ATGTACACAGGGTCATCGGCATCGACGAAGCCACCTTGGGATCGGAGGAGTCGGATGTCCCCATGCAGTAACTTTGATTACTGCTTTACCGAAGAGACAGAGGAGAAAACCCTTGAAATGGGGACGTTGGAAACACCCGAAATGGGGAAGTTGGAAACACCCTTTTTGATTGCTGCAAAAAATGGTGTGACTGAAATGGTGGAGGAGATCCTTAAAACTTTTCCGGTGGCCATCCGGGACACTAACGCAGAGGGAAAGAACGCGGTTTTGTTAGCTGCAGAGAACAGGCAACTATTTGTGCTCCAACTCTTACTGAAGAGAAATATACTGACCAGAGACGTGTTCAGCCAAGTGGATAATGCAGGGAATAATGCGTTTCATCTAGCAGCAGCAGCAATGTTAGAAGCCAAAAACCCTTGCCATGATCTTGGGCCTGCGTTACAAATGCAATGGGAAATCAAATGGTTTGAGTTCGTAAAATGCTCCCTGCCAACACAATTCCTTCCCCTCTACAACAACAAAAACCAGACTGCAAGGGATATTTTCAGCGAATCACACGCTAAGCTAGTCAAAGACGGAGGAGAATGGCTCACCAAGACTTCTCAGAACTGCTCGGTCTTGGGCGGCCTTGTCGCTACGGTTGCCTTTGCCACTGCTACTGCAGTTCCTGGGGGCATCAGAGAGGTTACTGGCACTCCAATCCTTGAAAACCAGGCGGCTTTTCAGGTGTTTGCCATCTCATCTCTCCTTGCGATGTGCTCCCTCCGTCATGTCCATGGCCATATTCCTGTCCATCCTGATGTCCCGGTTCCCCGAAGGGGCTTTTGGAAAGCAGCTGCCTATCAAGTTTTTAGTGGGCTTAACAACGCTCTTCTTGTCCATCCTTTCGATGCTGGTTTCATTTTGCGCTGGACATTTCTTCATGCTGAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

312

Amino Acids

34.41

Weight (kDa)

6.31

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 263
AciI CCGC 3 cut(s) 263, 624, 716
AclWI GGATC 2 cut(s) 48, 202
AcoI YGGCCR 2 cut(s) 228, 778
AcuI CTGAAG 1 cut(s) 335
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 818
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 2 cut(s) 116, 707
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 2 cut(s) 667, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 808, 840
AluBI AGCT 3 cut(s) 275, 565, 836
AluI AGCT 3 cut(s) 275, 565, 836
Alw21I GWGCWC 2 cut(s) 303, 761
Alw26I GTCTC 2 cut(s) 89, 330
AlwI GGATC 2 cut(s) 48, 202
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 4 cut(s) 228, 431, 625, 778
ApeKI GCWGC 7 cut(s) 173, 275, 386, 389, 392, 833, 836
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 1 cut(s) 431
AsuC2I CCSGG 2 cut(s) 236, 806
AsuHPI GGTGA 1 cut(s) 583
BalI TGGCCA 2 cut(s) 230, 780
Bbv12I GWGCWC 2 cut(s) 303, 761
BbvI GCAGC 7 cut(s) 160, 262, 398, 401, 404, 823, 845
BccI CCATC 4 cut(s) 239, 743, 801, 888
BciT130I CCWGG 2 cut(s) 669, 713
BcnI CCSGG 2 cut(s) 236, 806
BcoDI GTCTC 2 cut(s) 89, 330
BfaI CTAG 2 cut(s) 383, 566
BfmI CTRYAG 2 cut(s) 276, 660
BisI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 834, 837
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 9 cut(s) 175, 277, 388, 391, 394, 626, 718, 835, 838
Bme1390I CCNGG 4 cut(s) 236, 669, 713, 806
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 4 cut(s) 236, 669, 713, 806
BmsI GCATC 3 cut(s) 29, 684, 880
BplI GAGNNNNNCTC 2 cut(s) 573, 605
Bpu1102I GCTNAGC 1 cut(s) 561
BpuMI CCSGG 2 cut(s) 236, 806
BsaJI CCNNGG 3 cut(s) 35, 668, 775
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 143, 144, 818
Bse1I ACTGG 1 cut(s) 694
Bse3DI GCAATG 2 cut(s) 402, 455
BseBI CCWGG 2 cut(s) 669, 713
BseDI CCNNGG 3 cut(s) 35, 668, 775
BseGI GGATG 4 cut(s) 60, 231, 793, 880
BseLI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 818
BseMI GCAATG 2 cut(s) 402, 455
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 694
BseRI GAGGAG 4 cut(s) 61, 116, 218, 594
BseXI GCAGC 7 cut(s) 160, 262, 398, 401, 404, 823, 845
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 4 cut(s) 230, 433, 627, 780
BsiHKAI GWGCWC 2 cut(s) 303, 761
BsiSI CCGG 3 cut(s) 224, 235, 806
BslFI GGGAC 4 cut(s) 43, 136, 251, 788
BslI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 818
BsmAI GTCTC 2 cut(s) 89, 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 4 cut(s) 43, 136, 251, 788
BsnI GGCC 4 cut(s) 230, 433, 627, 780
Bsp1286I GDGCHC 2 cut(s) 303, 761
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 40, 207, 424
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 3 cut(s) 263, 624, 716
BspANI GGCC 4 cut(s) 230, 433, 627, 780
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 1 cut(s) 810
BspMAI CTGCAG 2 cut(s) 280, 664
BspPI GGATC 2 cut(s) 48, 202
BspQI GCTCTTC 1 cut(s) 876
BsrDI GCAATG 2 cut(s) 402, 455
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 694
BssECI CCNNGG 3 cut(s) 35, 668, 775
BssMI GATC 3 cut(s) 40, 207, 424
BssT1I CCWWGG 2 cut(s) 35, 775
Bst2UI CCWGG 2 cut(s) 669, 713
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 3 cut(s) 87, 311, 876
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 561, 603
BstDSI CCRYGG 1 cut(s) 775
BstF5I GGATG 4 cut(s) 60, 231, 793, 880
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 3 cut(s) 43, 210, 427
BstMAI GTCTC 2 cut(s) 89, 330
BstMBI GATC 3 cut(s) 40, 207, 424
BstMWI GCNNNNNNNGC 2 cut(s) 392, 641
BstNI CCWGG 2 cut(s) 669, 713
BstSCI CCNGG 4 cut(s) 234, 667, 711, 804
BstSFI CTRYAG 2 cut(s) 276, 660
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 7 cut(s) 160, 262, 398, 401, 404, 823, 845
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 4 cut(s) 230, 433, 627, 780
BtgI CCRYGG 1 cut(s) 775
BtgZI GCGATG 1 cut(s) 767
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 4 cut(s) 60, 231, 793, 880
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 4
CviAII CATG 5 cut(s) 63, 422, 770, 776, 923
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 561, 603
DpnI GATC 3 cut(s) 42, 209, 426
DpnII GATC 3 cut(s) 40, 207, 424
EaeI YGGCCR 2 cut(s) 228, 778
Eam1104I CTCTTC 3 cut(s) 87, 311, 876
EarI CTCTTC 3 cut(s) 87, 311, 876
Eco130I CCWWGG 2 cut(s) 35, 775
Eco57I CTGAAG 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 667, 711
EcoT14I CCWWGG 2 cut(s) 35, 775
ErhI CCWWGG 2 cut(s) 35, 775
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 5 cut(s) 66, 425, 773, 779, 926
FaiI YATR 7 cut(s) 64, 326, 423, 771, 777, 783, 924
FaqI GGGAC 4 cut(s) 43, 136, 251, 788
FatI CATG 5 cut(s) 62, 421, 769, 775, 922
Fnu4HI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 834, 837
FokI GGATG 4 cut(s) 67, 218, 780, 867
Fsp4HI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 834, 837
FspBI CTAG 2 cut(s) 383, 566
GlaI GCGC 1 cut(s) 908
GluI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 834, 837
HaeIII GGCC 4 cut(s) 230, 433, 627, 780
HapII CCGG 3 cut(s) 224, 235, 806
HhaI GCGC 1 cut(s) 909
Hin1II CATG 5 cut(s) 66, 425, 773, 779, 926
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HinfI GANTC 2 cut(s) 49, 551
HpaII CCGG 3 cut(s) 224, 235, 806
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 45, 54, 606, 680
Hpy188III TCNNGA 1 cut(s) 797
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 512, 810, 923
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 125, 339
HpyCH4V TGCA 7 cut(s) 66, 176, 278, 362, 448, 533, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 641
HpyF3I CTNAG 2 cut(s) 561, 603
HpySE526I ACGT 2 cut(s) 125, 339
Hsp92II CATG 5 cut(s) 66, 425, 773, 779, 926
HspAI GCGC 1 cut(s) 907
Kzo9I GATC 3 cut(s) 40, 207, 424
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 3 cut(s) 306, 488, 764
Lsp1109I GCAGC 7 cut(s) 160, 262, 398, 401, 404, 823, 845
LweI GCATC 3 cut(s) 29, 684, 880
MaeI CTAG 2 cut(s) 383, 566
MaeII ACGT 2 cut(s) 125, 339
MaeIII GTNAC 4 cut(s) 68, 187, 438, 685
MalI GATC 3 cut(s) 42, 209, 426
MboI GATC 3 cut(s) 40, 207, 424
MboII GAAGA 4 cut(s) 104, 328, 863, 911
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 303, 761
MlsI TGGCCA 2 cut(s) 230, 780
MluCI AATT 1 cut(s) 497
MluNI TGGCCA 2 cut(s) 230, 780
MlyI GAGTC 1 cut(s) 58
MmeI TCCRAC 4 cut(s) 32, 108, 132, 328
MnlI CCTC 8 cut(s) 39, 94, 196, 244, 518, 572, 676, 773
Mox20I TGGCCA 2 cut(s) 230, 780
MscI TGGCCA 2 cut(s) 230, 780
MseI TTAA 2 cut(s) 213, 861
MslI CAYNNNNRTG 2 cut(s) 774, 798
Msp20I TGGCCA 2 cut(s) 230, 780
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 3 cut(s) 224, 235, 806
MspR9I CCNGG 4 cut(s) 236, 669, 713, 806
MvaI CCWGG 2 cut(s) 669, 713
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 392, 641
NciI CCSGG 2 cut(s) 236, 806
NcoI CCATGG 1 cut(s) 775
NdeII GATC 3 cut(s) 40, 207, 424
NlaIII CATG 5 cut(s) 66, 425, 773, 779, 926
NlaIV GGNNCC 1 cut(s) 810
NmuCI GTSAC 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PfeI GAWTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 9 cut(s) 175, 277, 388, 391, 394, 626, 718, 835, 838
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Psp6I CCWGG 2 cut(s) 667, 711
PspGI CCWGG 2 cut(s) 667, 711
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 1 cut(s) 431
PstI CTGCAG 2 cut(s) 280, 664
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 774, 798
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 213, 861
SatI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 834, 837
Sau3AI GATC 3 cut(s) 40, 207, 424
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 4 cut(s) 236, 669, 713, 806
SduI GDGCHC 2 cut(s) 303, 761
SetI ASST 8 cut(s) 37, 128, 277, 342, 567, 687, 729, 838
SfaNI GCATC 3 cut(s) 29, 684, 880
SfcI CTRYAG 2 cut(s) 276, 660
SmiMI CAYNNNNRTG 2 cut(s) 774, 798
Sse9I AATT 1 cut(s) 497
SsiI CCGC 3 cut(s) 263, 624, 716
SspMI CTAG 2 cut(s) 383, 566
StyD4I CCNGG 4 cut(s) 234, 667, 711, 804
StyI CCWWGG 2 cut(s) 35, 775
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 128, 342
TaqI TCGA 2 cut(s) 23, 888
TaqII GACCGA 1 cut(s) 604
TasI AATT 1 cut(s) 497
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 627, 719
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 2 cut(s) 213, 861
Tru9I TTAA 2 cut(s) 213, 861
TscAI CASTG 1 cut(s) 658
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 7 cut(s) 173, 275, 386, 389, 392, 833, 836
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 368, 889, 911
TspGWI ACGGA 2 cut(s) 591, 755
TspRI CASTG 1 cut(s) 658
XspI CTAG 2 cut(s) 383, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.