Prupe.8G235300_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000723
Physical Location & Seq
Reverse (-)
20836793 .. 20840506
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G235300.3

Sequence Viewer

Length: 2211 bp
ATGGCTTCTTCTGTCGCGGATGACATGATCAAGAATATGGACACCGAGAAGATCAAGAAATACTTGTTCCAAATAGCCATGAAAAGCCAATGGAAAGAAGTTGTGAGAACCTATAGGCTGAACCCGGAGGCTCACAAGGCGAAGATCACCAGGACAGGTGACACAGCATTGCACATAGCAGTGTCCGACCGGCAAGAAGATCATGTTGAAGAACTGGTAAAATTAATTTCTCCTGATAAGCTCGAAAGGCAAAACGAGCGAGGGAACACCCCTCTCCATTTTGCCGCATCAATGGGGAATTTGAGGATGTGTGCATGCATTGCCGAACGCCATCCGTTGCTGGTTGGTGCTCCTAACGACGACAACGAGACTCCTCTCTTCTTGGCCGCTCTCCATGGTAAAAAAAATGCCTTCTTATGCCTCCACTACATCTACATCAAGAGCCCAGATCGTGAGCAGAAACGCTTCAACTATTGTAGGAGGAAGGACGGCGACACTATCTTGCATTGTGCAATTTCTGGGGACTACTTTGATCTGGCATTTCAGATAATTAAACTTTATACAGATCTTGCTAATAGTGTGAATGAGCAAGGGATTTCCCCTCTCCATCTTCTGGCCGCTAAGCCTTCTGCCTTCAAAAGTGGCAGCCACCTTAGCCCAACCGACAGAATCATATACCACTGTATATATGTGGATGAGCTCAGGTTGGAGCGACCGGATAGCTATAATCAGGGGATAATTAAGACGTTCAAAGAGGAAAAGGATCCAAACTATCCAGAAAACTACCAGACATGCATCAACTTCATTAGGTTGTTCTGGAATTTGATTCGCCTTGTTAGCTCGAGCTGTCAGAAAGCAGATCCTGAGACGCCTGTTGAACCCAAGAGTCACCAATCATTTGATTCATTTCCTGCTAACTACCGCATCTGCTTTGAGTATGTCAAACTTTTATCAAAGGCAATGCTGATTGTTCTTGGACTAGGGTCTACGCAGATAAGAAAGATAAGGGAAAAGAAAGAAAAGCACAAATGGTCAGTTCAGATCATGAATGAACTGCTAACACGTACTCCCATGTACTTGTATGAGGACGAAGACAGCGGTATGAATCCGGCGCCCCGCAAAGACAAGGAAGAGACACTGCCTTATGAAATTGTCGATGGCGGTGACGTCATCATGGGACGTGAAAGCATTATTGAACTTCCAAAAGATGATGATAACAAGGAGATTGGAGAAGGGTCATCAGGAGAAGCGACAGAGAAGAAGACTGTTAAGTTATTGGCAAAGAAGGAGACACCGATATTGATTGCAGCGAAAAGTGGTGTGACTGAAATGGTGGAGAAGATCCTGGAACTTTTTCCGGTGGCCATCCACGACACGAACACAGAGAAAAAGAATGTGGTGCTGTTGGCGGTGGAGAACAGGCAGCCCCATGTGTACCAGCTCTTGCTAAAGAGGAATGTTTTGAAAGACAGTGTGTTCGGTAAAGTGGATGACCAAGGAAACAGTGCTTTGCATCTCGCAGCAAAGCTAGAGGAGCATAAGCCTTGGCTCATTCCTGGAGCTGCGCTGCAAATGCAATGGGAAATCAAATGGTATGAGTACGTGAAAAACTCCATGCCACTGCACTTCTTTGCCCGCCACAATAGAATAGGCCAGACGGCAAGGGACCTCTTCACCGAATCCCACAAAAATATCGTGAAAGACGGAGGGCAGTGGCTCACCAACACTTCCGAGTCCTGCTCCGTCGTCGCCGCCCTCATTGCCACCGTGGCCTTCGCTACCGCAACCACCGTCCCCGGCGGCATCAAAGAGGACAGCGGCAAACCGACCCTCGAAAACCAGCCAGCTTTCGACGTGTTCGCCATTGCATCACTGGTGGCCCTCTGCTTCTCCGTCACGGCCATGGTGATGTTCCTGTCCATTCTGACGTCACGGTATCAAGAGAAGGACTTCGGCAAAGGCCTGCCCAGGAAGCTCCTGGTAGGGCTGACGTCGCTGTTCGTGTCCATAGCTTCCATGCTGGTTTCTTTCTGCGCGGGGCATTTCTTTGTGCTTAAAGATAAGCTGAAATACGCTGTGTTTCCGGTTTATGCCCTCACCTGCTTGCCTGTGACGTTTTTTGCGATGGTGCAGTTTCCGCTCTACGTTGATCTCATTTGGGCTACCTTTAAGAAGGTACCGCGGAGGAGTTACAAGGCTGCTTCGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

737

Amino Acids

83.38

Weight (kDa)

8.43

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2108
AatII GACGTC 3 cut(s) 1170, 1931, 1994
Acc36I ACCTGC 1 cut(s) 2108
Acc65I GGTACC 1 cut(s) 2176
AccB1I GGYRCC 2 cut(s) 1111, 2176
AccB7I CCANNNNNTGG 1 cut(s) 613
AccBSI CCGCTC 2 cut(s) 389, 2140
AccI GTMKAC 1 cut(s) 986
AccII CGCG 3 cut(s) 17, 2036, 2182
AclWI GGATC 4 cut(s) 758, 771, 854, 1336
AcoI YGGCCR 4 cut(s) 384, 615, 1362, 1899
AcsI RAATTY 2 cut(s) 298, 820
AcyI GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
AfaI GTAC 5 cut(s) 1066, 1076, 1436, 1601, 2178
AfiI CCNNNNNNNGG 1 cut(s) 613
AflIII ACRYGT 2 cut(s) 1061, 1854
AgsI TTSAA 7 cut(s) 209, 469, 637, 751, 878, 1196, 1465
AjiI CACGTC 2 cut(s) 1181, 1855
AjnI CCWGG 5 cut(s) 149, 1344, 1555, 1967, 1977
Alw21I GWGCWC 2 cut(s) 352, 702
Alw26I GTCTC 4 cut(s) 362, 860, 1127, 1283
AlwI GGATC 4 cut(s) 758, 771, 854, 1336
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 841
AoxI GGCC 8 cut(s) 384, 615, 1362, 1651, 1770, 1878, 1899, 1960
ApeKI GCWGC 7 cut(s) 645, 1307, 1423, 1520, 1562, 1567, 2198
ApoI RAATTY 2 cut(s) 298, 820
ArsI GACNNNNNNTTYG 2 cut(s) 1460, 1492
AseI ATTAAT 1 cut(s) 224
Asp700I GAANNNNTTC 3 cut(s) 464, 1353, 1949
Asp718I GGTACC 1 cut(s) 2176
AspLEI GCGC 3 cut(s) 1114, 1567, 2036
AspS9I GGNCC 2 cut(s) 1666, 1879
AsuC2I CCSGG 2 cut(s) 125, 1797
AsuHPI GGTGA 8 cut(s) 139, 170, 881, 1175, 1666, 1711, 1918, 2089
AvaI CYCGRG 1 cut(s) 841
AvaII GGWCC 1 cut(s) 1666
BalI TGGCCA 1 cut(s) 1364
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 2 cut(s) 1111, 2176
BanII GRGCYC 2 cut(s) 446, 702
BbsI GAAGAC 2 cut(s) 1098, 1268
Bbv12I GWGCWC 2 cut(s) 352, 702
BbvI GCAGC 7 cut(s) 657, 1319, 1435, 1532, 1549, 1554, 2185
BccI CCATC 5 cut(s) 339, 615, 1151, 1373, 2119
BceAI ACGGC 3 cut(s) 505, 1674, 1914
BciT130I CCWGG 5 cut(s) 151, 1346, 1557, 1969, 1979
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 2 cut(s) 125, 1797
BcoDI GTCTC 4 cut(s) 362, 860, 1127, 1283
BfaI CTAG 2 cut(s) 980, 1529
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 1115
BfuAI ACCTGC 1 cut(s) 2108
BglI GCCNNNNNGGC 1 cut(s) 1769
BglII AGATCT 1 cut(s) 565
BlpI GCTNAGC 1 cut(s) 621
Bme1390I CCNGG 7 cut(s) 125, 151, 1346, 1557, 1797, 1969, 1979
Bme18I GGWCC 1 cut(s) 1666
BmeT110I CYCGRG 1 cut(s) 841
BmgBI CACGTC 2 cut(s) 1181, 1855
BmgT120I GGNCC 2 cut(s) 1666, 1879
BmiI GGNNCC 4 cut(s) 765, 1113, 1667, 2178
BmrFI CCNGG 7 cut(s) 125, 151, 1346, 1557, 1797, 1969, 1979
BmsI GCATC 6 cut(s) 296, 804, 933, 1522, 1812, 1877
BoxI GACNNNNGTC 1 cut(s) 982
BpiI GAAGAC 2 cut(s) 1098, 1268
BplI GAGNNNNNCTC 2 cut(s) 1724, 1756
BpmI CTGGAG 1 cut(s) 1578
Bpu10I CCTNAGC 2 cut(s) 653, 701
Bpu1102I GCTNAGC 1 cut(s) 621
BpuMI CCSGG 2 cut(s) 125, 1797
BsaAI YACGTR 2 cut(s) 1064, 1603
BsaHI GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
BsaJI CCNNGG 8 cut(s) 394, 1495, 1544, 1767, 1795, 1902, 1967, 2180
BsaWI WCCGGW 3 cut(s) 715, 1357, 2083
BsaXI ACNNNNNCTCC 2 cut(s) 1051, 1081
Bsc4I CCNNNNNNNGG 1 cut(s) 613
Bse118I RCCGGY 1 cut(s) 189
Bse1I ACTGG 2 cut(s) 219, 1878
Bse3DI GCAATG 6 cut(s) 167, 318, 966, 1583, 1758, 1863
BseBI CCWGG 5 cut(s) 151, 1346, 1557, 1969, 1979
BseDI CCNNGG 8 cut(s) 394, 1495, 1544, 1767, 1795, 1902, 1967, 2180
BseGI GGATG 6 cut(s) 25, 312, 331, 700, 1365, 1495
BseLI CCNNNNNNNGG 1 cut(s) 613
BseMI GCAATG 6 cut(s) 167, 318, 966, 1583, 1758, 1863
BseMII CTCAG 2 cut(s) 715, 855
BseNI ACTGG 2 cut(s) 219, 1878
BseRI GAGGAG 3 cut(s) 363, 1547, 2200
BseXI GCAGC 7 cut(s) 657, 1319, 1435, 1532, 1549, 1554, 2185
BsgI GTGCAG 2 cut(s) 1607, 2150
Bsh1236I CGCG 3 cut(s) 17, 2036, 2182
Bsh1285I CGRYCG 2 cut(s) 190, 716
BshFI GGCC 8 cut(s) 386, 617, 1364, 1653, 1772, 1880, 1901, 1962
BshNI GGYRCC 2 cut(s) 1111, 2176
BsiEI CGRYCG 2 cut(s) 190, 716
BsiHKAI GWGCWC 2 cut(s) 352, 702
BsiHKCI CYCGRG 1 cut(s) 841
BsiSI CCGG 7 cut(s) 125, 190, 716, 1109, 1358, 1797, 2084
BslFI GGGAC 4 cut(s) 536, 1191, 1679, 1778
BslI CCNNNNNNNGG 1 cut(s) 613
BsmAI GTCTC 4 cut(s) 362, 860, 1127, 1283
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 4 cut(s) 536, 1191, 1679, 1778
BsnI GGCC 8 cut(s) 386, 617, 1364, 1653, 1772, 1880, 1901, 1962
BsoBI CYCGRG 1 cut(s) 841
Bsp1286I GDGCHC 3 cut(s) 352, 446, 702
Bsp1720I GCTNAGC 1 cut(s) 621
Bsp19I CCATGG 2 cut(s) 394, 1902
BspANI GGCC 8 cut(s) 386, 617, 1364, 1653, 1772, 1880, 1901, 1962
BspCNI CTCAG 2 cut(s) 714, 856
BspFNI CGCG 3 cut(s) 17, 2036, 2182
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 4 cut(s) 765, 1113, 1667, 2178
BspMI ACCTGC 1 cut(s) 2108
BspPI GGATC 4 cut(s) 758, 771, 854, 1336
BspT107I GGYRCC 2 cut(s) 1111, 2176
BsrBI CCGCTC 2 cut(s) 389, 2140
BsrDI GCAATG 6 cut(s) 167, 318, 966, 1583, 1758, 1863
BsrFI RCCGGY 1 cut(s) 189
BsrI ACTGG 2 cut(s) 219, 1878
BssAI RCCGGY 1 cut(s) 189
BssECI CCNNGG 8 cut(s) 394, 1495, 1544, 1767, 1795, 1902, 1967, 2180
BssNI GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
BssT1I CCWWGG 4 cut(s) 394, 1495, 1544, 1902
Bst2UI CCWGG 5 cut(s) 151, 1346, 1557, 1969, 1979
Bst4CI ACNGT 7 cut(s) 683, 1267, 1472, 1505, 1768, 1792, 1935
Bst6I CTCTTC 3 cut(s) 383, 1125, 1676
BstACI GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBAI YACGTR 2 cut(s) 1064, 1603
BstC8I GCNNGC 5 cut(s) 316, 1636, 1845, 1964, 2105
BstDEI CTNAG 4 cut(s) 621, 653, 701, 864
BstDSI CCRYGG 4 cut(s) 394, 1767, 1902, 2180
BstF5I GGATG 6 cut(s) 25, 312, 331, 700, 1365, 1495
BstFNI CGCG 3 cut(s) 17, 2036, 2182
BstH2I RGCGCY 1 cut(s) 1115
BstHHI GCGC 3 cut(s) 1114, 1567, 2036
BstMAI GTCTC 4 cut(s) 362, 860, 1127, 1283
BstMCI CGRYCG 2 cut(s) 190, 716
BstNI CCWGG 5 cut(s) 151, 1346, 1557, 1969, 1979
BstNSI RCATGY 2 cut(s) 318, 795
BstPAI GACNNNNGTC 1 cut(s) 982
BstSCI CCNGG 7 cut(s) 123, 149, 1344, 1555, 1795, 1967, 1977
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 3 cut(s) 17, 2036, 2182
BstV1I GCAGC 7 cut(s) 657, 1319, 1435, 1532, 1549, 1554, 2185
BstV2I GAAGAC 2 cut(s) 1098, 1268
BstX2I RGATCY 4 cut(s) 565, 763, 859, 1341
BstYI RGATCY 4 cut(s) 565, 763, 859, 1341
BsuRI GGCC 8 cut(s) 386, 617, 1364, 1653, 1772, 1880, 1901, 1962
BtgI CCRYGG 4 cut(s) 394, 1767, 1902, 2180
BtgZI GCGATG 1 cut(s) 2138
BtrI CACGTC 2 cut(s) 1181, 1855
BtsCI GGATG 6 cut(s) 25, 312, 331, 700, 1365, 1495
BtsI GCAGTG 4 cut(s) 186, 1136, 1619, 1718
BtsIMutI CAGTG 8 cut(s) 186, 679, 1136, 1477, 1510, 1619, 1718, 1871
BveI ACCTGC 1 cut(s) 2108
Cac8I GCNNGC 5 cut(s) 316, 1636, 1845, 1964, 2105
CaiI CAGNNNCTG 1 cut(s) 863
CciI TCATGA 1 cut(s) 1044
CfoI GCGC 3 cut(s) 1114, 1567, 2036
Cfr10I RCCGGY 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 1666, 1879
Cfr42I CCGCGG 1 cut(s) 2183
CseI GACGC 1 cut(s) 877
Csp6I GTAC 5 cut(s) 1065, 1075, 1435, 1600, 2177
CviQI GTAC 5 cut(s) 1065, 1075, 1435, 1600, 2177
DdeI CTNAG 4 cut(s) 621, 653, 701, 864
DinI GGCGCC 1 cut(s) 1113
EaeI YGGCCR 4 cut(s) 384, 615, 1362, 1899
Eam1104I CTCTTC 3 cut(s) 383, 1125, 1676
EarI CTCTTC 3 cut(s) 383, 1125, 1676
Ecl136II GAGCTC 1 cut(s) 700
Eco130I CCWWGG 4 cut(s) 394, 1495, 1544, 1902
Eco147I AGGCCT 1 cut(s) 1962
Eco24I GRGCYC 2 cut(s) 446, 702
Eco47I GGWCC 1 cut(s) 1666
Eco53kI GAGCTC 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 841
EcoICRI GAGCTC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 1666
EcoRII CCWGG 5 cut(s) 149, 1344, 1555, 1967, 1977
EcoT14I CCWWGG 4 cut(s) 394, 1495, 1544, 1902
EcoT22I ATGCAT 2 cut(s) 320, 797
EcoT38I GRGCYC 2 cut(s) 446, 702
EgeI GGCGCC 1 cut(s) 1113
EheI GGCGCC 1 cut(s) 1113
ErhI CCWWGG 4 cut(s) 394, 1495, 1544, 1902
Esp3I CGTCTC 1 cut(s) 860
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 4 cut(s) 536, 1191, 1679, 1778
FauI CCCGC 3 cut(s) 1124, 1643, 2029
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 1 cut(s) 986
FokI GGATG 6 cut(s) 32, 318, 319, 707, 1352, 1502
FriOI GRGCYC 2 cut(s) 446, 702
FspBI CTAG 2 cut(s) 980, 1529
GlaI GCGC 3 cut(s) 1113, 1566, 2035
GsuI CTGGAG 1 cut(s) 1578
HaeII RGCGCY 1 cut(s) 1115
HaeIII GGCC 8 cut(s) 386, 617, 1364, 1653, 1772, 1880, 1901, 1962
HapII CCGG 7 cut(s) 125, 190, 716, 1109, 1358, 1797, 2084
HgaI GACGC 1 cut(s) 877
HhaI GCGC 3 cut(s) 1114, 1567, 2036
Hin1I GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
Hin6I GCGC 3 cut(s) 1112, 1565, 2034
HinP1I GCGC 3 cut(s) 1112, 1565, 2034
HinfI GANTC 8 cut(s) 370, 669, 826, 886, 902, 1105, 1679, 1733
HpaII CCGG 7 cut(s) 125, 190, 716, 1109, 1358, 1797, 2084
HphI GGTGA 8 cut(s) 139, 170, 881, 1175, 1666, 1711, 1918, 2089
Hpy166II GTNNAC 2 cut(s) 987, 1435
Hpy188I TCNGA 6 cut(s) 187, 546, 852, 1041, 1732, 1926
Hpy8I GTNNAC 2 cut(s) 987, 1435
Hpy99I CGWCG 5 cut(s) 362, 1748, 1751, 1856, 1996
HpyAV CCTTC 9 cut(s) 421, 478, 636, 643, 1226, 1279, 1783, 1939, 2167
HpyCH4III ACNGT 7 cut(s) 683, 1267, 1472, 1505, 1768, 1792, 1935
HpyF3I CTNAG 4 cut(s) 621, 653, 701, 864
Hsp92I GRCGYC 5 cut(s) 869, 1112, 1167, 1928, 1991
HspAI GCGC 3 cut(s) 1112, 1565, 2034
KasI GGCGCC 1 cut(s) 1111
KpnI GGTACC 1 cut(s) 2180
Ksp22I TGATCA 1 cut(s) 27
KspI CCGCGG 1 cut(s) 2183
LmnI GCTCC 6 cut(s) 355, 709, 1534, 1559, 1745, 1980
Lsp1109I GCAGC 7 cut(s) 657, 1319, 1435, 1532, 1549, 1554, 2185
LweI GCATC 6 cut(s) 296, 804, 933, 1522, 1812, 1877
MaeI CTAG 2 cut(s) 980, 1529
MaeIII GTNAC 8 cut(s) 158, 887, 1163, 1321, 1894, 1929, 2110, 2189
MbiI CCGCTC 2 cut(s) 389, 2140
MflI RGATCY 4 cut(s) 565, 763, 859, 1341
MhlI GDGCHC 3 cut(s) 352, 446, 702
MlsI TGGCCA 1 cut(s) 1364
MluCI AATT 8 cut(s) 221, 225, 298, 513, 549, 738, 820, 1149
MluNI TGGCCA 1 cut(s) 1364
Mly113I GGCGCC 1 cut(s) 1112
MlyI GAGTC 3 cut(s) 364, 895, 1742
MmeI TCCRAC 2 cut(s) 210, 687
Mox20I TGGCCA 1 cut(s) 1364
Mph1103I ATGCAT 2 cut(s) 320, 797
MroXI GAANNNNTTC 3 cut(s) 464, 1353, 1949
MscI TGGCCA 1 cut(s) 1364
MseI TTAA 6 cut(s) 224, 552, 741, 1269, 2055, 2169
MslI CAYNNNNRTG 3 cut(s) 179, 1901, 1907
Msp20I TGGCCA 1 cut(s) 1364
MspA1I CMGCKG 3 cut(s) 1098, 1818, 2182
MspI CCGG 7 cut(s) 125, 190, 716, 1109, 1358, 1797, 2084
MspR9I CCNGG 7 cut(s) 125, 151, 1346, 1557, 1797, 1969, 1979
MvaI CCWGG 5 cut(s) 151, 1346, 1557, 1969, 1979
MvnI CGCG 3 cut(s) 17, 2036, 2182
NarI GGCGCC 1 cut(s) 1112
NciI CCSGG 2 cut(s) 125, 1797
NcoI CCATGG 2 cut(s) 394, 1902
NlaIV GGNNCC 4 cut(s) 765, 1113, 1667, 2178
NmuCI GTSAC 7 cut(s) 158, 887, 1163, 1321, 1894, 1929, 2110
NsiI ATGCAT 2 cut(s) 320, 797
NspI RCATGY 2 cut(s) 318, 795
PaeI GCATGC 1 cut(s) 318
PaeR7I CTCGAG 1 cut(s) 841
PagI TCATGA 1 cut(s) 1044
PaqCI CACCTGC 1 cut(s) 2108
PceI AGGCCT 1 cut(s) 1962
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 2120
PdmI GAANNNNTTC 3 cut(s) 464, 1353, 1949
PfeI GAWTC 5 cut(s) 669, 826, 902, 1105, 1679
PflMI CCANNNNNTGG 1 cut(s) 613
PfoI TCCNGGA 2 cut(s) 1344, 1555
PleI GAGTC 3 cut(s) 364, 894, 1741
PluTI GGCGCC 1 cut(s) 1115
PpsI GAGTC 3 cut(s) 364, 894, 1741
Ppu21I YACGTR 2 cut(s) 1064, 1603
PpuMI RGGWCCY 1 cut(s) 1666
PshAI GACNNNNGTC 1 cut(s) 982
PshBI ATTAAT 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 702
Psp5II RGGWCCY 1 cut(s) 1666
Psp6I CCWGG 5 cut(s) 149, 1344, 1555, 1967, 1977
PspGI CCWGG 5 cut(s) 149, 1344, 1555, 1967, 1977
PspN4I GGNNCC 4 cut(s) 765, 1113, 1667, 2178
PspPI GGNCC 2 cut(s) 1666, 1879
PspPPI RGGWCCY 1 cut(s) 1666
PspXI VCTCGAGB 1 cut(s) 841
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 4 cut(s) 565, 763, 859, 1341
RsaI GTAC 5 cut(s) 1066, 1076, 1436, 1601, 2178
RsaNI GTAC 5 cut(s) 1065, 1075, 1435, 1600, 2177
RseI CAYNNNNRTG 3 cut(s) 179, 1901, 1907
SacI GAGCTC 1 cut(s) 702
SacII CCGCGG 1 cut(s) 2183
SaqAI TTAA 6 cut(s) 224, 552, 741, 1269, 2055, 2169
Sau96I GGNCC 2 cut(s) 1666, 1879
SchI GAGTC 3 cut(s) 364, 895, 1742
ScrFI CCNGG 7 cut(s) 125, 151, 1346, 1557, 1797, 1969, 1979
SduI GDGCHC 3 cut(s) 352, 446, 702
SfaNI GCATC 6 cut(s) 296, 804, 933, 1522, 1812, 1877
SfcI CTRYAG 1 cut(s) 112
SfoI GGCGCC 1 cut(s) 1113
Sfr274I CTCGAG 1 cut(s) 841
Sfr303I CCGCGG 1 cut(s) 2183
SgrBI CCGCGG 1 cut(s) 2183
SinI GGWCC 1 cut(s) 1666
SlaI CTCGAG 1 cut(s) 841
SmiMI CAYNNNNRTG 3 cut(s) 179, 1901, 1907
SmlI CTYRAG 1 cut(s) 841
SmoI CTYRAG 1 cut(s) 841
SphI GCATGC 1 cut(s) 318
Sse9I AATT 8 cut(s) 221, 225, 298, 513, 549, 738, 820, 1149
SseBI AGGCCT 1 cut(s) 1962
SspDI GGCGCC 1 cut(s) 1111
SspMI CTAG 2 cut(s) 980, 1529
SstI GAGCTC 1 cut(s) 702
StuI AGGCCT 1 cut(s) 1962
StyD4I CCNGG 7 cut(s) 123, 149, 1344, 1555, 1795, 1967, 1977
StyI CCWWGG 4 cut(s) 394, 1495, 1544, 1902
TaaI ACNGT 7 cut(s) 683, 1267, 1472, 1505, 1768, 1792, 1935
TaqI TCGA 5 cut(s) 243, 842, 1155, 1833, 1851
TasI AATT 8 cut(s) 221, 225, 298, 513, 549, 738, 820, 1149
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 6 cut(s) 287, 389, 620, 1754, 1803, 1821
TfiI GAWTC 5 cut(s) 669, 826, 902, 1105, 1679
Tru1I TTAA 6 cut(s) 224, 552, 741, 1269, 2055, 2169
Tru9I TTAA 6 cut(s) 224, 552, 741, 1269, 2055, 2169
TscAI CASTG 8 cut(s) 186, 686, 1143, 1477, 1510, 1626, 1718, 1878
TseFI GTSAC 7 cut(s) 158, 887, 1163, 1321, 1894, 1929, 2110
TseI GCWGC 7 cut(s) 645, 1307, 1423, 1520, 1562, 1567, 2198
Tsp45I GTSAC 7 cut(s) 158, 887, 1163, 1321, 1894, 1929, 2110
TspDTI ATGAA 7 cut(s) 95, 793, 894, 1061, 1065, 1118, 1161
TspGWI ACGGA 4 cut(s) 324, 1719, 1732, 1882
TspRI CASTG 8 cut(s) 186, 686, 1143, 1477, 1510, 1626, 1718, 1878
Van91I CCANNNNNTGG 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 1666
VspI ATTAAT 1 cut(s) 224
XapI RAATTY 2 cut(s) 298, 820
XceI RCATGY 2 cut(s) 318, 795
XcmI CCANNNNNNNNNTGG 2 cut(s) 1870, 1975
XhoI CTCGAG 1 cut(s) 841
XmiI GTMKAC 1 cut(s) 986
XmnI GAANNNNTTC 3 cut(s) 464, 1353, 1949
XspI CTAG 2 cut(s) 980, 1529
ZraI GACGTC 3 cut(s) 1168, 1929, 1992
Zsp2I ATGCAT 2 cut(s) 320, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.