ankyrin repeat-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 761 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 601 |
| AciI | CCGC | 2 cut(s) | 618, 721 |
| AclWI | GGATC | 1 cut(s) | 146 |
| AcoI | YGGCCR | 1 cut(s) | 184 |
| AcuI | CTGAAG | 1 cut(s) | 710 |
| AcyI | GRCGYC | 1 cut(s) | 365 |
| AfaI | GTAC | 1 cut(s) | 430 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 601, 721 |
| AgsI | TTSAA | 2 cut(s) | 203, 625 |
| AhlI | ACTAGT | 1 cut(s) | 208 |
| AjnI | CCWGG | 1 cut(s) | 216 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 696, 728 |
| AluBI | AGCT | 5 cut(s) | 152, 235, 542, 578, 688 |
| AluI | AGCT | 5 cut(s) | 152, 235, 542, 578, 688 |
| Alw26I | GTCTC | 3 cut(s) | 356, 419, 476 |
| AlwI | GGATC | 1 cut(s) | 146 |
| AoxI | GGCC | 1 cut(s) | 184 |
| ApeKI | GCWGC | 3 cut(s) | 278, 281, 380 |
| AsuHPI | GGTGA | 1 cut(s) | 133 |
| BalI | TGGCCA | 1 cut(s) | 186 |
| BanII | GRGCYC | 1 cut(s) | 727 |
| BbsI | GAAGAC | 1 cut(s) | 435 |
| BbvI | GCAGC | 3 cut(s) | 265, 293, 367 |
| BccI | CCATC | 5 cut(s) | 176, 470, 500, 603, 778 |
| BceAI | ACGGC | 1 cut(s) | 174 |
| BciT130I | CCWGG | 1 cut(s) | 218 |
| BcoDI | GTCTC | 3 cut(s) | 356, 419, 476 |
| BcuI | ACTAGT | 1 cut(s) | 208 |
| BfaI | CTAG | 3 cut(s) | 209, 557, 770 |
| BfmI | CTRYAG | 1 cut(s) | 279 |
| BfuAI | ACCTGC | 1 cut(s) | 761 |
| BglII | AGATCT | 1 cut(s) | 553 |
| BisI | GCNGC | 3 cut(s) | 279, 282, 381 |
| BlsI | GCNGC | 3 cut(s) | 280, 283, 382 |
| Bme1390I | CCNGG | 1 cut(s) | 218 |
| BmiI | GGNNCC | 1 cut(s) | 726 |
| BmrFI | CCNGG | 1 cut(s) | 218 |
| BmsI | GCATC | 3 cut(s) | 54, 391, 765 |
| BpiI | GAAGAC | 1 cut(s) | 435 |
| BpuEI | CTTGAG | 1 cut(s) | 774 |
| BsaHI | GRCGYC | 1 cut(s) | 365 |
| BsaJI | CCNNGG | 2 cut(s) | 388, 647 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 601, 721 |
| Bse118I | RCCGGY | 1 cut(s) | 26 |
| Bse3DI | GCAATG | 2 cut(s) | 161, 312 |
| BseBI | CCWGG | 1 cut(s) | 218 |
| BseDI | CCNNGG | 2 cut(s) | 388, 647 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 601, 721 |
| BseMI | GCAATG | 2 cut(s) | 161, 312 |
| BseRI | GAGGAG | 1 cut(s) | 245 |
| BseXI | GCAGC | 3 cut(s) | 265, 293, 367 |
| BseYI | CCCAGC | 1 cut(s) | 578 |
| BshFI | GGCC | 1 cut(s) | 186 |
| BsiSI | CCGG | 1 cut(s) | 27 |
| BslFI | GGGAC | 1 cut(s) | 524 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 601, 721 |
| BsmAI | GTCTC | 3 cut(s) | 356, 419, 476 |
| BsmBI | CGTCTC | 1 cut(s) | 356 |
| BsmFI | GGGAC | 1 cut(s) | 524 |
| BsnI | GGCC | 1 cut(s) | 186 |
| Bsp1286I | GDGCHC | 1 cut(s) | 727 |
| Bsp143I | GATC | 3 cut(s) | 138, 553, 774 |
| Bsp19I | CCATGG | 1 cut(s) | 388 |
| BspACI | CCGC | 2 cut(s) | 618, 721 |
| BspANI | GGCC | 1 cut(s) | 186 |
| BspLI | GGNNCC | 1 cut(s) | 726 |
| BspMAI | CTGCAG | 1 cut(s) | 283 |
| BspMI | ACCTGC | 1 cut(s) | 761 |
| BspPI | GGATC | 1 cut(s) | 146 |
| BsrDI | GCAATG | 2 cut(s) | 161, 312 |
| BsrFI | RCCGGY | 1 cut(s) | 26 |
| BssAI | RCCGGY | 1 cut(s) | 26 |
| BssECI | CCNNGG | 2 cut(s) | 388, 647 |
| BssMI | GATC | 3 cut(s) | 138, 553, 774 |
| BssNI | GRCGYC | 1 cut(s) | 365 |
| BssT1I | CCWWGG | 2 cut(s) | 388, 647 |
| Bst2UI | CCWGG | 1 cut(s) | 218 |
| Bst4CI | ACNGT | 3 cut(s) | 38, 464, 671 |
| Bst6I | CTCTTC | 1 cut(s) | 377 |
| BstACI | GRCGYC | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 2 cut(s) | 123, 723 |
| BstDEI | CTNAG | 1 cut(s) | 641 |
| BstDSI | CCRYGG | 1 cut(s) | 388 |
| BstKTI | GATC | 3 cut(s) | 141, 556, 777 |
| BstMAI | GTCTC | 3 cut(s) | 356, 419, 476 |
| BstMBI | GATC | 3 cut(s) | 138, 553, 774 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 118, 131, 158 |
| BstNI | CCWGG | 1 cut(s) | 218 |
| BstSCI | CCNGG | 1 cut(s) | 216 |
| BstSFI | CTRYAG | 1 cut(s) | 279 |
| BstV1I | GCAGC | 3 cut(s) | 265, 293, 367 |
| BstV2I | GAAGAC | 1 cut(s) | 435 |
| BstX2I | RGATCY | 1 cut(s) | 553 |
| BstYI | RGATCY | 1 cut(s) | 553 |
| BsuRI | GGCC | 1 cut(s) | 186 |
| BtgI | CCRYGG | 1 cut(s) | 388 |
| BtsI | GCAGTG | 1 cut(s) | 180 |
| BtsIMutI | CAGTG | 3 cut(s) | 43, 180, 667 |
| BveI | ACCTGC | 1 cut(s) | 761 |
| Cac8I | GCNNGC | 2 cut(s) | 123, 723 |
| Cfr10I | RCCGGY | 1 cut(s) | 26 |
| CseI | GACGC | 1 cut(s) | 373 |
| CsiI | ACCWGGT | 1 cut(s) | 216 |
| Csp6I | GTAC | 1 cut(s) | 429 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 407, 442 |
| CviAII | CATG | 4 cut(s) | 22, 31, 73, 389 |
| CviQI | GTAC | 1 cut(s) | 429 |
| DdeI | CTNAG | 1 cut(s) | 641 |
| DpnI | GATC | 3 cut(s) | 140, 555, 776 |
| DpnII | GATC | 3 cut(s) | 138, 553, 774 |
| EaeI | YGGCCR | 1 cut(s) | 184 |
| Eam1104I | CTCTTC | 1 cut(s) | 377 |
| EarI | CTCTTC | 1 cut(s) | 377 |
| Eco130I | CCWWGG | 2 cut(s) | 388, 647 |
| Eco24I | GRGCYC | 1 cut(s) | 727 |
| Eco57I | CTGAAG | 1 cut(s) | 710 |
| EcoRII | CCWGG | 1 cut(s) | 216 |
| EcoT14I | CCWWGG | 2 cut(s) | 388, 647 |
| EcoT38I | GRGCYC | 1 cut(s) | 727 |
| ErhI | CCWWGG | 2 cut(s) | 388, 647 |
| Esp3I | CGTCTC | 1 cut(s) | 356 |
| FaeI | CATG | 4 cut(s) | 25, 34, 76, 392 |
| FaqI | GGGAC | 1 cut(s) | 524 |
| FatI | CATG | 4 cut(s) | 21, 30, 72, 388 |
| FauI | CCCGC | 1 cut(s) | 714 |
| Fnu4HI | GCNGC | 3 cut(s) | 279, 282, 381 |
| FriOI | GRGCYC | 1 cut(s) | 727 |
| Fsp4HI | GCNGC | 3 cut(s) | 279, 282, 381 |
| FspBI | CTAG | 3 cut(s) | 209, 557, 770 |
| GluI | GCNGC | 3 cut(s) | 279, 282, 381 |
| GsaI | CCCAGC | 1 cut(s) | 582 |
| HaeIII | GGCC | 1 cut(s) | 186 |
| HapII | CCGG | 1 cut(s) | 27 |
| HgaI | GACGC | 1 cut(s) | 373 |
| Hin1I | GRCGYC | 1 cut(s) | 365 |
| Hin1II | CATG | 4 cut(s) | 25, 34, 76, 392 |
| HpaII | CCGG | 1 cut(s) | 27 |
| HphI | GGTGA | 1 cut(s) | 133 |
| Hpy166II | GTNNAC | 1 cut(s) | 214 |
| Hpy188I | TCNGA | 3 cut(s) | 101, 468, 534 |
| Hpy188III | TCNNGA | 4 cut(s) | 49, 64, 434, 778 |
| Hpy8I | GTNNAC | 1 cut(s) | 214 |
| HpyAV | CCTTC | 4 cut(s) | 339, 415, 624, 685 |
| HpyCH4III | ACNGT | 3 cut(s) | 38, 464, 671 |
| HpyCH4IV | ACGT | 1 cut(s) | 706 |
| HpyCH4V | TGCA | 6 cut(s) | 166, 281, 306, 312, 493, 756 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 118, 131, 158 |
| HpyF3I | CTNAG | 1 cut(s) | 641 |
| HpySE526I | ACGT | 1 cut(s) | 706 |
| Hsp92I | GRCGYC | 1 cut(s) | 365 |
| Hsp92II | CATG | 4 cut(s) | 25, 34, 76, 392 |
| Kzo9I | GATC | 3 cut(s) | 138, 553, 774 |
| LmnI | GCTCC | 3 cut(s) | 232, 651, 730 |
| Lsp1109I | GCAGC | 3 cut(s) | 265, 293, 367 |
| LweI | GCATC | 3 cut(s) | 54, 391, 765 |
| MabI | ACCWGGT | 1 cut(s) | 216 |
| MaeI | CTAG | 3 cut(s) | 209, 557, 770 |
| MaeII | ACGT | 1 cut(s) | 706 |
| MaeIII | GTNAC | 1 cut(s) | 707 |
| MalI | GATC | 3 cut(s) | 140, 555, 776 |
| MboI | GATC | 3 cut(s) | 138, 553, 774 |
| MboII | GAAGA | 9 cut(s) | 101, 215, 364, 435, 485, 590, 710, 713, 793 |
| MflI | RGATCY | 1 cut(s) | 553 |
| MhlI | GDGCHC | 1 cut(s) | 727 |
| MlsI | TGGCCA | 1 cut(s) | 186 |
| MluCI | AATT | 3 cut(s) | 93, 457, 562 |
| MluNI | TGGCCA | 1 cut(s) | 186 |
| MmeI | TCCRAC | 1 cut(s) | 446 |
| MnlI | CCTC | 7 cut(s) | 184, 223, 248, 276, 378, 462, 600 |
| Mox20I | TGGCCA | 1 cut(s) | 186 |
| MscI | TGGCCA | 1 cut(s) | 186 |
| MseI | TTAA | 2 cut(s) | 561, 570 |
| MslI | CAYNNNNRTG | 1 cut(s) | 173 |
| Msp20I | TGGCCA | 1 cut(s) | 186 |
| MspA1I | CMGCKG | 2 cut(s) | 152, 721 |
| MspI | CCGG | 1 cut(s) | 27 |
| MspR9I | CCNGG | 1 cut(s) | 218 |
| MvaI | CCWGG | 1 cut(s) | 218 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 118, 131, 158 |
| NcoI | CCATGG | 1 cut(s) | 388 |
| NdeII | GATC | 3 cut(s) | 138, 553, 774 |
| NlaIII | CATG | 4 cut(s) | 25, 34, 76, 392 |
| NlaIV | GGNNCC | 1 cut(s) | 726 |
| NmuCI | GTSAC | 1 cut(s) | 707 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 712 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 601 |
| PkrI | GCNGC | 3 cut(s) | 280, 283, 382 |
| Psp6I | CCWGG | 1 cut(s) | 216 |
| PspFI | CCCAGC | 1 cut(s) | 578 |
| PspGI | CCWGG | 1 cut(s) | 216 |
| PspN4I | GGNNCC | 1 cut(s) | 726 |
| PstI | CTGCAG | 1 cut(s) | 283 |
| PsuI | RGATCY | 1 cut(s) | 553 |
| PvuII | CAGCTG | 1 cut(s) | 152 |
| RsaI | GTAC | 1 cut(s) | 430 |
| RsaNI | GTAC | 1 cut(s) | 429 |
| RseI | CAYNNNNRTG | 1 cut(s) | 173 |
| SaqAI | TTAA | 2 cut(s) | 561, 570 |
| SatI | GCNGC | 3 cut(s) | 279, 282, 381 |
| Sau3AI | GATC | 3 cut(s) | 138, 553, 774 |
| ScrFI | CCNGG | 1 cut(s) | 218 |
| SduI | GDGCHC | 1 cut(s) | 727 |
| SexAI | ACCWGGT | 1 cut(s) | 216 |
| SfaNI | GCATC | 3 cut(s) | 54, 391, 765 |
| SfcI | CTRYAG | 1 cut(s) | 279 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 173 |
| SmlI | CTYRAG | 1 cut(s) | 789 |
| SmoI | CTYRAG | 1 cut(s) | 789 |
| SpeI | ACTAGT | 1 cut(s) | 208 |
| Sse9I | AATT | 3 cut(s) | 93, 457, 562 |
| SsiI | CCGC | 2 cut(s) | 618, 721 |
| SspMI | CTAG | 3 cut(s) | 209, 557, 770 |
| StyD4I | CCNGG | 1 cut(s) | 216 |
| StyI | CCWWGG | 2 cut(s) | 388, 647 |
| TaaI | ACNGT | 3 cut(s) | 38, 464, 671 |
| TaiI | ACGT | 1 cut(s) | 709 |
| TaqI | TCGA | 1 cut(s) | 681 |
| TasI | AATT | 3 cut(s) | 93, 457, 562 |
| TatI | WGTACW | 1 cut(s) | 428 |
| Tru1I | TTAA | 2 cut(s) | 561, 570 |
| Tru9I | TTAA | 2 cut(s) | 561, 570 |
| TscAI | CASTG | 3 cut(s) | 43, 180, 674 |
| TseFI | GTSAC | 1 cut(s) | 707 |
| TseI | GCWGC | 3 cut(s) | 278, 281, 380 |
| Tsp45I | GTSAC | 1 cut(s) | 707 |
| TspDTI | ATGAA | 2 cut(s) | 89, 588 |
| TspGWI | ACGGA | 1 cut(s) | 704 |
| TspRI | CASTG | 3 cut(s) | 43, 180, 674 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 601 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 28 |
| XspI | CTAG | 3 cut(s) | 209, 557, 770 |