pycom03g20510

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000723
Physical Location & Seq
Reverse (-)
21023368 .. 21024311
944 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20510.4

Sequence Viewer

Length: 564 bp
ATGCACTACATCTGTAGCCCCAATGGTCTCCCGAACTACAATTATTGTAGGAGAAATGATGGTGATACCATCTTGCATTGTGCAATTGCCGGGGACTACTTTGATTTGGCATTTCAGATAATTCACTTGTATGAAGATCTTGTTAATTATGTCAATGCGGAAGGCTTTTCCCCTCTCCATCTTCTGGCTACCAAGCCTTCTGCCTTCAAAAGCGGTAGCCACCTCGGCCGATTCCAAAATATTATTTACTACTGTATTTATGTTGATGAGCTCAAGGCGGAGCGCTGGGAGCCATATAATGAGGGAATAATTAAGACATTCAAAGAGGAAAAGAATCCCAAATATCCAGAAAACTACCATACATGCATCAACTTCATTAGGTTGTTCGGGACATCAATTCTCAGCGTTATTCAAAACCATGCCAATCAGAGAAAAGAAACACAATCAGCAGGTACAGGGAGCGTCCCTCAAGTACAGAAAGGAGAAAAAAAATCAGCAGATCCAGAGAACCCTGCAGAACCTAAAAAGCAAACTCTCAACACTTCAAATCCAGGTAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

188

Amino Acids

21.3

Weight (kDa)

6.65

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 440
AccB7I CCANNNNNTGG 1 cut(s) 184
AciI CCGC 3 cut(s) 158, 213, 278
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 226
AfaI GTAC 2 cut(s) 454, 474
AfeI AGCGCT 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 184
AgsI TTSAA 4 cut(s) 208, 322, 413, 546
AjnI CCWGG 1 cut(s) 550
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 284
AoxI GGCC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 285
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 74
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 273
BccI CCATC 3 cut(s) 53, 77, 186
BciT130I CCWGG 1 cut(s) 552
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 2 cut(s) 13, 513
BfoI RGCGCY 1 cut(s) 286
BfuAI ACCTGC 1 cut(s) 440
BglI GCCNNNNNGGC 1 cut(s) 225
BglII AGATCT 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 91, 552
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 2 cut(s) 91, 552
BmsI GCATC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 451, 483
BpuEI CTTGAG 2 cut(s) 257, 453
BpuMI CCSGG 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 90, 223
Bsc4I CCNNNNNNNGG 1 cut(s) 184
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 90, 223
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 415
BseX3I CGGCCG 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 3 cut(s) 107, 403, 449
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 3 cut(s) 107, 403, 449
BsnI GGCC 1 cut(s) 228
Bso31I GGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 136, 499
BspACI CCGC 3 cut(s) 158, 213, 278
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 291
BspMAI CTGCAG 1 cut(s) 517
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 90, 223
BssMI GATC 2 cut(s) 136, 499
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 286
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 2 cut(s) 139, 502
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 136, 499
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 225, 289
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 2 cut(s) 89, 550
BstSFI CTRYAG 2 cut(s) 13, 513
BstX2I RGATCY 2 cut(s) 136, 499
BstYI RGATCY 2 cut(s) 136, 499
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 1 cut(s) 228
BveI ACCTGC 1 cut(s) 440
CfoI GCGC 1 cut(s) 285
CseI GACGC 1 cut(s) 451
Csp6I GTAC 2 cut(s) 453, 473
CviAII CATG 2 cut(s) 363, 419
CviJI RGCY 8 cut(s) 18, 165, 188, 196, 219, 228, 271, 292
CviKI_1 RGCY 8 cut(s) 18, 165, 188, 196, 219, 228, 271, 292
CviQI GTAC 2 cut(s) 453, 473
DdeI CTNAG 1 cut(s) 401
DpnI GATC 2 cut(s) 138, 501
DpnII GATC 2 cut(s) 136, 499
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
EciI GGCGGA 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 271
EclXI CGGCCG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 32
Eco47III AGCGCT 1 cut(s) 284
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 368
EcoT38I GRGCYC 1 cut(s) 273
FaeI CATG 2 cut(s) 366, 422
FaiI YATR 8 cut(s) 132, 150, 261, 295, 297, 360, 364, 420
FaqI GGGAC 3 cut(s) 107, 403, 449
FatI CATG 2 cut(s) 362, 418
FriOI GRGCYC 1 cut(s) 273
GlaI GCGC 1 cut(s) 284
GsaI CCCAGC 1 cut(s) 289
HaeII RGCGCY 1 cut(s) 286
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 366, 422
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HinfI GANTC 2 cut(s) 231, 334
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 74
Hpy188I TCNGA 2 cut(s) 117, 429
Hpy188III TCNNGA 4 cut(s) 31, 347, 388, 503
HpyAV CCTTC 3 cut(s) 155, 207, 214
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 5 cut(s) 4, 76, 83, 366, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 289
HpyF3I CTNAG 1 cut(s) 401
Hsp92II CATG 2 cut(s) 366, 422
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 136, 499
LmnI GCTCC 3 cut(s) 280, 289, 459
LpnPI CCDG 9 cut(s) 103, 170, 271, 360, 435, 441, 516, 525, 537
LweI GCATC 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 138, 501
MboI GATC 2 cut(s) 136, 499
MboII GAAGA 2 cut(s) 146, 173
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 2 cut(s) 136, 499
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 6 cut(s) 40, 84, 120, 145, 309, 396
MnlI CCTC 5 cut(s) 183, 233, 295, 319, 477
Mph1103I ATGCAT 1 cut(s) 368
MseI TTAA 2 cut(s) 144, 312
MslI CAYNNNNRTG 1 cut(s) 129
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 91, 552
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 225, 289
NciI CCSGG 1 cut(s) 91
NdeII GATC 2 cut(s) 136, 499
NlaIII CATG 2 cut(s) 366, 422
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 368
NspI RCATGY 1 cut(s) 366
PfeI GAWTC 2 cut(s) 231, 334
PflMI CCANNNNNTGG 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 1 cut(s) 291
PstI CTGCAG 1 cut(s) 517
PsuI RGATCY 2 cut(s) 136, 499
RsaI GTAC 2 cut(s) 454, 474
RsaNI GTAC 2 cut(s) 453, 473
RseI CAYNNNNRTG 1 cut(s) 129
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 2 cut(s) 144, 312
Sau3AI GATC 2 cut(s) 136, 499
ScrFI CCNGG 2 cut(s) 91, 552
SduI GDGCHC 1 cut(s) 273
SetI ASST 6 cut(s) 225, 273, 383, 454, 523, 556
SfaNI GCATC 1 cut(s) 375
SfcI CTRYAG 2 cut(s) 13, 513
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 2 cut(s) 272, 468
SmoI CTYRAG 2 cut(s) 272, 468
Sse9I AATT 6 cut(s) 40, 84, 120, 145, 309, 396
SsiI CCGC 3 cut(s) 158, 213, 278
SspI AATATT 1 cut(s) 241
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 89, 550
TaaI ACNGT 1 cut(s) 254
TasI AATT 6 cut(s) 40, 84, 120, 145, 309, 396
TatI WGTACW 1 cut(s) 472
TfiI GAWTC 2 cut(s) 231, 334
Tru1I TTAA 2 cut(s) 144, 312
Tru9I TTAA 2 cut(s) 144, 312
TspDTI ATGAA 2 cut(s) 147, 364
Van91I CCANNNNNTGG 1 cut(s) 184
XceI RCATGY 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.