pycom03g20520

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000723
Physical Location & Seq
Reverse (-)
21039781 .. 21041388
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20520.2

Sequence Viewer

Length: 1086 bp
ATGTACACAGGGTCATCGGCATCGACGAAGCCACCTTGGGCTCGGAGGAGTCGGATGTCCCCATGCAGTAACTTTGATTACTGCTTTACCGAAGAGACAGAGGAGAAAACCCTTGAAATGGGGACGTTGGAAACACCCGAAATGGGGAAGTTGGAAACACCCTTTTTGATTGCTGCAAAAAATGGTGTGATTGAAATGGTGGAGAAGATCCTTGAAATTTTTCCGGTGGCCATCCGGGACACTAACGCAGAGGGAAAGAACGCGGTTTTGTTAGCTGCAGAGAACAGGCAACTATTTGTGCTCCAACTCTTACTGAAGAGAAATATACTGACCAGAGACGTGTTCAGCCAAGTGGATAATGCAGGGAATAATGCGTTTCATCTCGCAGCAGCAGCAATGTTAGAAGCCAAAAACCCTTGCCATGATCTTGGGCCTGCGTTACAAATGCAATGGGAAATCAAATGGTTTGAGTTCGTAAAATGCTCCCTGCCAACACAATTCCTTCCCCTCTACAACAACAAAAACCAGACTGCAGGGGATATTTTCAGCGAATCACACGCTAAGCTAGTCAAAGACGGAGGAGAATGGCTCACCAAGACTTCTCAGAACTGCTCGGTCATGGGCGGCCTTGTCGCTACGGTTGCCTTTGCCACTGCTACTGCAGTTCCTGGGGGCATCAGAGAGGTTACTGGCACTCCAATCTTTGAAAACCAGGCGGCTTTTGAGGTGTTTGCCATCTCATCTCTCCTTGCGATGTGCTCCTCCGTCATGTCCATGGCCATATTCCTGTCCATCCTGATGTCCCGGTTCCCCGAAGGGGCTTTTGGAAAGCAGCTGCCTATCAAGTTTTTAGTGGGCTTAACAACGCTCTTCTTGTCCATCCTTTCGATGCTGGTTTCATTTTGCGCTGGACATTTCTTCATGCTAAAGGATAAGCTCAAACACTTTGCCGTGCCAGTTTATCTAATGGCCTGCATGCCTCTAGCTTTATTTTCTATCATCCAGCTTCCACTCTATTTTAATCTGATATATATGAACTTTGCAAAGGTTCCATACTTCTATCGGAACGCCGAGGCTACAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

362

Amino Acids

40.03

Weight (kDa)

6.54

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 263
AciI CCGC 3 cut(s) 263, 624, 716
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 2 cut(s) 228, 777
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 335
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 817
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 4 cut(s) 116, 194, 215, 707
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 2 cut(s) 667, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AluBI AGCT 6 cut(s) 275, 565, 835, 937, 986, 1006
AluI AGCT 6 cut(s) 275, 565, 835, 937, 986, 1006
Alw21I GWGCWC 2 cut(s) 303, 761
Alw26I GTCTC 2 cut(s) 89, 330
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 5 cut(s) 228, 431, 625, 777, 969
ApeKI GCWGC 7 cut(s) 173, 275, 386, 389, 392, 832, 835
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 1 cut(s) 431
AsuC2I CCSGG 2 cut(s) 236, 805
AsuHPI GGTGA 1 cut(s) 583
BalI TGGCCA 2 cut(s) 230, 779
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 303, 761
BbvI GCAGC 7 cut(s) 160, 262, 398, 401, 404, 822, 844
BccI CCATC 4 cut(s) 239, 743, 800, 887
BceAI ACGGC 1 cut(s) 935
BciT130I CCWGG 2 cut(s) 669, 713
BcnI CCSGG 2 cut(s) 236, 805
BcoDI GTCTC 2 cut(s) 89, 330
BfaI CTAG 2 cut(s) 566, 983
BfmI CTRYAG 4 cut(s) 276, 531, 660, 1077
BisI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 833, 836
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 9 cut(s) 175, 277, 388, 391, 394, 626, 718, 834, 837
Bme1390I CCNGG 4 cut(s) 236, 669, 713, 805
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 2 cut(s) 809, 1050
BmrFI CCNGG 4 cut(s) 236, 669, 713, 805
BmsI GCATC 3 cut(s) 29, 684, 879
BplI GAGNNNNNCTC 2 cut(s) 573, 605
Bpu1102I GCTNAGC 1 cut(s) 561
BpuMI CCSGG 2 cut(s) 236, 805
BsaJI CCNNGG 4 cut(s) 35, 668, 774, 1071
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 143, 144, 817
Bse1I ACTGG 2 cut(s) 694, 956
Bse3DI GCAATG 2 cut(s) 402, 455
BseBI CCWGG 2 cut(s) 669, 713
BseDI CCNNGG 4 cut(s) 35, 668, 774, 1071
BseGI GGATG 5 cut(s) 60, 231, 792, 879, 999
BseLI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 817
BseMI GCAATG 2 cut(s) 402, 455
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 2 cut(s) 694, 956
BseRI GAGGAG 4 cut(s) 61, 116, 594, 751
BseXI GCAGC 7 cut(s) 160, 262, 398, 401, 404, 822, 844
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 5 cut(s) 230, 433, 627, 779, 971
BsiHKAI GWGCWC 2 cut(s) 303, 761
BsiSI CCGG 3 cut(s) 224, 235, 805
BslFI GGGAC 4 cut(s) 43, 136, 251, 787
BslI CCNNNNNNNGG 4 cut(s) 118, 143, 144, 817
BsmAI GTCTC 2 cut(s) 89, 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 4 cut(s) 43, 136, 251, 787
BsnI GGCC 5 cut(s) 230, 433, 627, 779, 971
Bsp1286I GDGCHC 3 cut(s) 43, 303, 761
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 207, 424
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 3 cut(s) 263, 624, 716
BspANI GGCC 5 cut(s) 230, 433, 627, 779, 971
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 809, 1050
BspMAI CTGCAG 3 cut(s) 280, 535, 664
BspPI GGATC 1 cut(s) 202
BspQI GCTCTTC 1 cut(s) 875
BsrDI GCAATG 2 cut(s) 402, 455
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 694, 956
BssECI CCNNGG 4 cut(s) 35, 668, 774, 1071
BssMI GATC 2 cut(s) 207, 424
BssT1I CCWWGG 2 cut(s) 35, 774
Bst2UI CCWGG 2 cut(s) 669, 713
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 3 cut(s) 87, 311, 875
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 435, 973, 977
BstDEI CTNAG 2 cut(s) 561, 603
BstDSI CCRYGG 1 cut(s) 774
BstF5I GGATG 5 cut(s) 60, 231, 792, 879, 999
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 2 cut(s) 210, 427
BstMAI GTCTC 2 cut(s) 89, 330
BstMBI GATC 2 cut(s) 207, 424
BstMWI GCNNNNNNNGC 2 cut(s) 392, 641
BstNI CCWGG 2 cut(s) 669, 713
BstNSI RCATGY 1 cut(s) 979
BstSCI CCNGG 4 cut(s) 234, 667, 711, 803
BstSFI CTRYAG 4 cut(s) 276, 531, 660, 1077
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 7 cut(s) 160, 262, 398, 401, 404, 822, 844
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 5 cut(s) 230, 433, 627, 779, 971
BtgI CCRYGG 1 cut(s) 774
BtgZI GCGATG 1 cut(s) 767
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 5 cut(s) 60, 231, 792, 879, 999
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 3 cut(s) 435, 973, 977
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 1 cut(s) 908
Cfr13I GGNCC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 4
CviAII CATG 7 cut(s) 63, 422, 619, 769, 775, 922, 976
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 561, 603
DpnI GATC 2 cut(s) 209, 426
DpnII GATC 2 cut(s) 207, 424
EaeI YGGCCR 2 cut(s) 228, 777
Eam1104I CTCTTC 3 cut(s) 87, 311, 875
EarI CTCTTC 3 cut(s) 87, 311, 875
Eco130I CCWWGG 2 cut(s) 35, 774
Eco24I GRGCYC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 667, 711
EcoT14I CCWWGG 2 cut(s) 35, 774
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 35, 774
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 7 cut(s) 66, 425, 622, 772, 778, 925, 979
FaqI GGGAC 4 cut(s) 43, 136, 251, 787
FatI CATG 7 cut(s) 62, 421, 618, 768, 774, 921, 975
Fnu4HI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 833, 836
FokI GGATG 5 cut(s) 67, 218, 779, 866, 986
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 833, 836
FspBI CTAG 2 cut(s) 566, 983
GlaI GCGC 1 cut(s) 907
GluI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 833, 836
HaeIII GGCC 5 cut(s) 230, 433, 627, 779, 971
HapII CCGG 3 cut(s) 224, 235, 805
HhaI GCGC 1 cut(s) 908
Hin1II CATG 7 cut(s) 66, 425, 622, 772, 778, 925, 979
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HinfI GANTC 2 cut(s) 49, 551
HpaII CCGG 3 cut(s) 224, 235, 805
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 45, 54, 606, 680, 1026, 1065
Hpy188III TCNNGA 1 cut(s) 796
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 512, 809
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 125, 339
HpyCH4V TGCA 9 cut(s) 66, 176, 278, 362, 448, 533, 662, 975, 1043
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 641
HpyF3I CTNAG 2 cut(s) 561, 603
HpySE526I ACGT 2 cut(s) 125, 339
Hsp92II CATG 7 cut(s) 66, 425, 622, 772, 778, 925, 979
HspAI GCGC 1 cut(s) 906
Kzo9I GATC 2 cut(s) 207, 424
LguI GCTCTTC 1 cut(s) 875
LmnI GCTCC 3 cut(s) 306, 488, 764
Lsp1109I GCAGC 7 cut(s) 160, 262, 398, 401, 404, 822, 844
LweI GCATC 3 cut(s) 29, 684, 879
MaeI CTAG 2 cut(s) 566, 983
MaeII ACGT 2 cut(s) 125, 339
MaeIII GTNAC 3 cut(s) 68, 438, 685
MalI GATC 2 cut(s) 209, 426
MboI GATC 2 cut(s) 207, 424
MboII GAAGA 5 cut(s) 104, 217, 328, 862, 910
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 3 cut(s) 43, 303, 761
MlsI TGGCCA 2 cut(s) 230, 779
MluCI AATT 2 cut(s) 216, 497
MluNI TGGCCA 2 cut(s) 230, 779
MlyI GAGTC 1 cut(s) 58
MmeI TCCRAC 4 cut(s) 32, 108, 132, 328
Mox20I TGGCCA 2 cut(s) 230, 779
MroXI GAANNNNTTC 1 cut(s) 219
MscI TGGCCA 2 cut(s) 230, 779
MseI TTAA 2 cut(s) 860, 1020
MslI CAYNNNNRTG 2 cut(s) 773, 797
Msp20I TGGCCA 2 cut(s) 230, 779
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 3 cut(s) 224, 235, 805
MspR9I CCNGG 4 cut(s) 236, 669, 713, 805
MvaI CCWGG 2 cut(s) 669, 713
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 392, 641
NciI CCSGG 2 cut(s) 236, 805
NcoI CCATGG 1 cut(s) 774
NdeII GATC 2 cut(s) 207, 424
NlaIII CATG 7 cut(s) 66, 425, 622, 772, 778, 925, 979
NlaIV GGNNCC 2 cut(s) 809, 1050
NspI RCATGY 1 cut(s) 979
PaeI GCATGC 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 875
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 9 cut(s) 175, 277, 388, 391, 394, 626, 718, 834, 837
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Psp6I CCWGG 2 cut(s) 667, 711
PspGI CCWGG 2 cut(s) 667, 711
PspN4I GGNNCC 2 cut(s) 809, 1050
PspPI GGNCC 1 cut(s) 431
PstI CTGCAG 3 cut(s) 280, 535, 664
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 773, 797
SapI GCTCTTC 1 cut(s) 875
SaqAI TTAA 2 cut(s) 860, 1020
SatI GCNGC 9 cut(s) 174, 276, 387, 390, 393, 625, 717, 833, 836
Sau3AI GATC 2 cut(s) 207, 424
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 4 cut(s) 236, 669, 713, 805
SduI GDGCHC 3 cut(s) 43, 303, 761
SfaNI GCATC 3 cut(s) 29, 684, 879
SfcI CTRYAG 4 cut(s) 276, 531, 660, 1077
SmiMI CAYNNNNRTG 2 cut(s) 773, 797
SphI GCATGC 1 cut(s) 979
Sse9I AATT 2 cut(s) 216, 497
SsiI CCGC 3 cut(s) 263, 624, 716
SspMI CTAG 2 cut(s) 566, 983
StyD4I CCNGG 4 cut(s) 234, 667, 711, 803
StyI CCWWGG 2 cut(s) 35, 774
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 128, 342
TaqI TCGA 2 cut(s) 23, 887
TaqII GACCGA 1 cut(s) 604
TasI AATT 2 cut(s) 216, 497
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 627, 719
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 2 cut(s) 860, 1020
Tru9I TTAA 2 cut(s) 860, 1020
TscAI CASTG 1 cut(s) 658
TseI GCWGC 7 cut(s) 173, 275, 386, 389, 392, 832, 835
TspDTI ATGAA 4 cut(s) 368, 888, 910, 1049
TspGWI ACGGA 2 cut(s) 591, 754
TspRI CASTG 1 cut(s) 658
XapI RAATTY 1 cut(s) 216
XceI RCATGY 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 566, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.