MD03G1258700.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000723
Physical Location & Seq
Reverse (-)
34571530 .. 34591129
19600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1258700.v1.1.491

Sequence Viewer

Length: 2487 bp
ATGTACCCAATAATAGATGTGGATTCTCTCACGGATGAGGAGATCAAGGGTATGGACACAGAGAAGATCAAGAGATACTTGTTCCAAATTGCCATGCGGAGCAAGTGGAAAGCAGTTGTTAGAATCTATAGGCTCAACAAGGAGGCTCACGGGGCAAAAATCACCAAGTCATGTGACACAGCACTACACGTAGCAGTATCTGATGGCCAAGAAGAACATGTTAAAGAGCTAGTAAATCAGATTACGGGAAAGGAGCTTGAAATTCAAAACGAGCGAGGGAATACCCCTCTTCACATCGCGGCATGGATGGGAAACGAGACAATGTGTTCGTTCCTTGCCAACGCTCATCCATCGTTGGTCACTACTTTTAATGTAGACAATGAGACTCCTCTATTCTTGGCTGCTGTCTATGGTAAAAAAGATGCCTTCTTATGCATGCACCGCATTTATACCCCTACCCTTGATGATCCTCGGAAACGCTACAATTATTGTAGGAGGAATAACGGTGATACCATCTTGCATTGTGCAATTTCCGGGGACTACTTTGATTTGGCATTTCAGATAATTCACTTGTATGAAGATCTTGTTAATTCTGTCAATGCGCAAGGCTTTTCCCCTCTCCATCTTCTGGCTACCAAGCCTTCTGCCTTCAAAAGCGGGAGCCACCTCAGCCGATTCCAAGAAATTATTTACCACTGTATATATGTTGATGAGCTCAAGGCGGAGCAAAGGAAACGATCTAATGAGGGGATAATTAAGACATTCAAAGAGGAAGAGAATCCCGAACATCCAGAAAACTACCAGGCATGCATCAACTTCATTAGGTTGTTCTGGAGAACAATTCGCCTTGTTACCAAAAAGGAAGACAATCAGAGAGAAGAAACACAATCAGCAGATACAGGGAACGTCCCCCAAGCACCTAATCGTACTCACACGATCAACACCATCGATCAGAGAGGAGAGAAAAAATCAGCAGATCCAGAGAACCCTATAGAACCTAGGTCGAAATCCAATGCTGAGCAAGGTCAAGGACACAAAAAGTTTCCCGGAAACTACAACACCTGCTTTGAGTTTGTCAAGCTTATATCAAAGGCACTGCTGATTGTTCTTGGCTTAGGGTCTACGGAGATAAGAAAGACAAGGGAAAAGAAAGAAAAACACAAATGGTCTGTTCAGATCATGACCGAACTCTTAGAACGAGCTGTCATGTACGCGTATGAAGATAGTGGTATGATTCCGCCGGTCACCGCACCACCTTCCCTCAAAGAAAAAGACAATGATGAGACCACACCTTATAACATTGCTGATGGCGGCGCCGTCCTTATGCCATCCGATAGCGCCCTCGTTGTCCCAATGCATCAACGGGAGGCTGAGCAGCCAAAGAATGGGGAAGAAAAAACCCCAGAAGGGCCAAAATCCATTGTTGATCAAGGTCAAGGAGACCAAAAGATTCCCGTAAACCACAACACCTGCTCTGAGTTTGTGAAGCTTTTATCAAAGGAAATGCTGATTGCTCTTGGCTTAGATAAAAAGACGGAGAATATAACCCCAAGCGTAAACACGGAAACGCCAATACTGATTGCTGCAAGAAATGGGGTGACTGAAATGGTGGAGAAGATCCTTGAACTTTTTCCAGTGGCAATACACGATATGAACAAAGAGAAAAAGAATATGGTGTTGCTGGCAGTAGAACACAGGCAACCCCATGTGTACAAGCTCTTGCTGAAAAGAAATATCCTGAAAGAAAGTGTGTTCGGTAGAGTGGATCATGAAGGGAATAGTGCATTACATCTAGCAGCAAAGTTGGGAGAACATAAGCCTTGGCTAATTCCTGGAGCTGCGCTGCAAATGCAATGGGAAATCAAATGGTATGAGTTTGTAAAAACCTCCATGCCACTACACTTCTTTGCCCGCCACAACGAGAAGGGCAGGACAGCAAAGGAAATCTTCACCGAAACCCACACCACAATCGTCAAGGCCGGAGGGGAGTGGCTGACCAACACCTCCGAGTCCTGCTCCGTCGTGGCTGCCCTCATTGCCACCGTTGCCTTCGCCACCTCAACCACAGTCCCCGGCGGCATCAAAGAAGAAAGCGGAACACCAACCCTAGAAAACCAGCCAGCTTTCGACATCTTTGCCATTGCCTCCCTGGTTGCCCTCTGCTTCTCCGTCACGGCCATGGTCATGTTCCTATCCATCTTAACGTCCCGGTACCAAGAGAAGGACTTCGGTAAGGACCTGCCAAGAAAGCTTTTGGTAGGGCTCACGTCACTGTTCGTGTCAATAGCTTCCATGTTGGTTTCGTTTTGTGCCGGACATTTTTTCGTGCTTAAAGATAAGCTCAAATACGCTGCATTTCCGGTTTACGCAATCACTTGCTTGCCAGTAACGTTTTTTGCCATGGCGCAGTTTCCATTGTACGTTGATCTGATTTGGGCTACTTTTAAGAAGGTACCACAGCGGAGTTACAAGATAACTCCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

829

Amino Acids

93.45

Weight (kDa)

7.36

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 114 4.1e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 62 - 142 7.2e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 62 - 112 1.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 526 - 604 2.3e-06 Ankyrin repeats (3 copies)
PGG PF13962 663 - 775 2.1e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1296
AarI CACCTGC 2 cut(s) 1070, 1478
Acc16I TGCGCA 1 cut(s) 603
Acc36I ACCTGC 3 cut(s) 1070, 1478, 2247
Acc65I GGTACC 2 cut(s) 2211, 2452
AccB1I GGYRCC 3 cut(s) 1313, 2211, 2452
AccB7I CCANNNNNTGG 2 cut(s) 628, 1385
AccI GTMKAC 2 cut(s) 375, 1121
AccII CGCG 2 cut(s) 299, 1214
AclI AACGTT 1 cut(s) 2390
AclWI GGATC 4 cut(s) 461, 971, 1612, 1773
AcoI YGGCCR 2 cut(s) 205, 2175
AcsI RAATTY 1 cut(s) 261
AcyI GRCGYC 1 cut(s) 1314
AfaI GTAC 7 cut(s) 5, 928, 1211, 1712, 2213, 2420, 2454
AfiI CCNNNNNNNGG 4 cut(s) 628, 1385, 1407, 2221
AflIII ACRYGT 3 cut(s) 187, 217, 1212
AgsI TTSAA 5 cut(s) 260, 266, 652, 766, 1625
AjiI CACGTC 1 cut(s) 2268
AjnI CCWGG 3 cut(s) 801, 1831, 2148
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 4 cut(s) 311, 377, 1277, 1434
AlwI GGATC 4 cut(s) 461, 971, 1612, 1773
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 4 cut(s) 205, 1409, 1977, 2175
ApeKI GCWGC 8 cut(s) 401, 1375, 1583, 1796, 1838, 1843, 2027, 2351
ApoI RAATTY 1 cut(s) 261
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
Asp700I GAANNNNTTC 2 cut(s) 1629, 2225
Asp718I GGTACC 2 cut(s) 2211, 2452
AspA2I CCTAGG 1 cut(s) 998
AspLEI GCGC 5 cut(s) 604, 1316, 1340, 1843, 2407
AspS9I GGNCC 2 cut(s) 1409, 2236
AsuC2I CCSGG 4 cut(s) 535, 1047, 2073, 2209
AsuHPI GGTGA 5 cut(s) 154, 518, 1237, 1609, 1942
AvaII GGWCC 1 cut(s) 2236
AvrII CCTAGG 1 cut(s) 998
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 3 cut(s) 1313, 2211, 2452
BanII GRGCYC 2 cut(s) 717, 2265
BbsI GAAGAC 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 717
BbvCI CCTCAGC 1 cut(s) 668
BbvI GCAGC 8 cut(s) 388, 1387, 1570, 1808, 1825, 1830, 2014, 2338
BccI CCATC 9 cut(s) 197, 301, 358, 521, 630, 953, 1301, 1336, 2204
BceAI ACGGC 2 cut(s) 1301, 2190
BcgI CGANNNNNNTGC 2 cut(s) 2117, 2151
BciT130I CCWGG 3 cut(s) 803, 1833, 2150
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 4 cut(s) 535, 1047, 2073, 2209
BcoDI GTCTC 4 cut(s) 311, 377, 1277, 1434
BfaI CTAG 4 cut(s) 230, 999, 1793, 2108
BfmI CTRYAG 2 cut(s) 127, 990
BfoI RGCGCY 2 cut(s) 1317, 1341
BfuAI ACCTGC 3 cut(s) 1070, 1478, 2247
BglII AGATCT 1 cut(s) 580
BlnI CCTAGG 1 cut(s) 998
BlpI GCTNAGC 2 cut(s) 1017, 1371
Bme1390I CCNGG 7 cut(s) 535, 803, 1047, 1833, 2073, 2150, 2209
Bme18I GGWCC 1 cut(s) 2236
BmgBI CACGTC 1 cut(s) 2268
BmgT120I GGNCC 2 cut(s) 1409, 2236
BmiI GGNNCC 4 cut(s) 662, 1315, 2213, 2454
BmrFI CCNGG 7 cut(s) 535, 803, 1047, 1833, 2073, 2150, 2209
BmsI GCATC 4 cut(s) 412, 819, 1366, 2088
BpiI GAAGAC 1 cut(s) 870
BplI GAGNNNNNCTC 2 cut(s) 2000, 2032
BpmI CTGGAG 2 cut(s) 853, 1854
Bpu10I CCTNAGC 2 cut(s) 668, 1114
Bpu1102I GCTNAGC 2 cut(s) 1017, 1371
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 4 cut(s) 535, 1047, 2073, 2209
Bsa29I ATCGAT 1 cut(s) 948
BsaAI YACGTR 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 1314
BsaI GGTCTC 2 cut(s) 1277, 1434
BsaJI CCNNGG 8 cut(s) 470, 534, 998, 1820, 2071, 2148, 2178, 2400
BsaWI WCCGGW 1 cut(s) 2359
Bsc4I CCNNNNNNNGG 4 cut(s) 628, 1385, 1407, 2221
Bse118I RCCGGY 1 cut(s) 1240
Bse1I ACTGG 2 cut(s) 1634, 2384
Bse3DI GCAATG 4 cut(s) 1299, 1859, 2034, 2139
BseBI CCWGG 3 cut(s) 803, 1833, 2150
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 8 cut(s) 470, 534, 998, 1820, 2071, 2148, 2178, 2400
BseGI GGATG 5 cut(s) 40, 312, 346, 787, 1328
BseLI CCNNNNNNNGG 4 cut(s) 628, 1385, 1407, 2221
BseMI GCAATG 4 cut(s) 1299, 1859, 2034, 2139
BseMII CTCAG 4 cut(s) 682, 1008, 1362, 1467
BseNI ACTGG 2 cut(s) 1634, 2384
BseRI GAGGAG 4 cut(s) 53, 378, 972, 2469
BseXI GCAGC 8 cut(s) 388, 1387, 1570, 1808, 1825, 1830, 2014, 2338
Bsh1236I CGCG 2 cut(s) 299, 1214
BshFI GGCC 4 cut(s) 207, 1411, 1979, 2177
BshNI GGYRCC 3 cut(s) 1313, 2211, 2452
BshVI ATCGAT 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 8 cut(s) 534, 1047, 1241, 1980, 2073, 2209, 2313, 2360
BslFI GGGAC 5 cut(s) 551, 893, 1334, 2054, 2191
BslI CCNNNNNNNGG 4 cut(s) 628, 1385, 1407, 2221
BsmAI GTCTC 4 cut(s) 311, 377, 1277, 1434
BsmFI GGGAC 5 cut(s) 551, 893, 1334, 2054, 2191
BsnI GGCC 4 cut(s) 207, 1411, 1979, 2177
Bso31I GGTCTC 2 cut(s) 1277, 1434
Bsp1286I GDGCHC 2 cut(s) 717, 2265
Bsp1407I TGTACA 1 cut(s) 1710
Bsp1720I GCTNAGC 2 cut(s) 1017, 1371
Bsp19I CCATGG 2 cut(s) 2178, 2400
BspANI GGCC 4 cut(s) 207, 1411, 1979, 2177
BspCNI CTCAG 4 cut(s) 681, 1009, 1363, 1468
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 2 cut(s) 299, 1214
BspHI TCATGA 2 cut(s) 1179, 1768
BspLI GGNNCC 4 cut(s) 662, 1315, 2213, 2454
BspMI ACCTGC 3 cut(s) 1070, 1478, 2247
BspPI GGATC 4 cut(s) 461, 971, 1612, 1773
BspT107I GGYRCC 3 cut(s) 1313, 2211, 2452
BspTNI GGTCTC 2 cut(s) 1277, 1434
BsrDI GCAATG 4 cut(s) 1299, 1859, 2034, 2139
BsrFI RCCGGY 1 cut(s) 1240
BsrGI TGTACA 1 cut(s) 1710
BsrI ACTGG 2 cut(s) 1634, 2384
BssAI RCCGGY 1 cut(s) 1240
BssECI CCNNGG 8 cut(s) 470, 534, 998, 1820, 2071, 2148, 2178, 2400
BssNI GRCGYC 1 cut(s) 1314
BssT1I CCWWGG 4 cut(s) 998, 1820, 2178, 2400
Bst2UI CCWGG 3 cut(s) 803, 1833, 2150
Bst4CI ACNGT 5 cut(s) 506, 698, 2044, 2068, 2274
Bst6I CTCTTC 2 cut(s) 294, 768
BstACI GRCGYC 1 cut(s) 1314
BstAUI TGTACA 1 cut(s) 1710
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 6 cut(s) 437, 808, 1683, 1912, 2121, 2381
BstDEI CTNAG 7 cut(s) 668, 1017, 1114, 1192, 1371, 1476, 1522
BstDSI CCRYGG 2 cut(s) 2178, 2400
BstEII GGTNACC 1 cut(s) 1243
BstF5I GGATG 5 cut(s) 40, 312, 346, 787, 1328
BstFNI CGCG 2 cut(s) 299, 1214
BstH2I RGCGCY 2 cut(s) 1317, 1341
BstHHI GCGC 5 cut(s) 604, 1316, 1340, 1843, 2407
BstMAI GTCTC 4 cut(s) 311, 377, 1277, 1434
BstMWI GCNNNNNNNGC 7 cut(s) 152, 441, 669, 1849, 2036, 2045, 2248
BstNI CCWGG 3 cut(s) 803, 1833, 2150
BstNSI RCATGY 3 cut(s) 221, 439, 810
BstPI GGTNACC 1 cut(s) 1243
BstSCI CCNGG 7 cut(s) 533, 801, 1045, 1831, 2071, 2148, 2207
BstSFI CTRYAG 2 cut(s) 127, 990
BstUI CGCG 2 cut(s) 299, 1214
BstV1I GCAGC 8 cut(s) 388, 1387, 1570, 1808, 1825, 1830, 2014, 2338
BstV2I GAAGAC 1 cut(s) 870
BstX2I RGATCY 3 cut(s) 580, 976, 1617
BstYI RGATCY 3 cut(s) 580, 976, 1617
Bsu15I ATCGAT 1 cut(s) 948
BsuRI GGCC 4 cut(s) 207, 1411, 1979, 2177
BsuTUI ATCGAT 1 cut(s) 948
BtgI CCRYGG 2 cut(s) 2178, 2400
BtgZI GCGATG 1 cut(s) 280
BtrI CACGTC 1 cut(s) 2268
BtsCI GGATG 5 cut(s) 40, 312, 346, 787, 1328
BtsI GCAGTG 1 cut(s) 1094
BtsIMutI CAGTG 4 cut(s) 694, 1094, 1641, 2270
BveI ACCTGC 3 cut(s) 1070, 1478, 2247
Cac8I GCNNGC 6 cut(s) 437, 808, 1683, 1912, 2121, 2381
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 2 cut(s) 1179, 1768
CfoI GCGC 5 cut(s) 604, 1316, 1340, 1843, 2407
Cfr10I RCCGGY 1 cut(s) 1240
Cfr13I GGNCC 2 cut(s) 1409, 2236
ClaI ATCGAT 1 cut(s) 948
Csp6I GTAC 7 cut(s) 4, 927, 1210, 1711, 2212, 2419, 2453
CviQI GTAC 7 cut(s) 4, 927, 1210, 1711, 2212, 2419, 2453
DdeI CTNAG 7 cut(s) 668, 1017, 1114, 1192, 1371, 1476, 1522
DinI GGCGCC 1 cut(s) 1315
EaeI YGGCCR 2 cut(s) 205, 2175
Eam1104I CTCTTC 2 cut(s) 294, 768
EarI CTCTTC 2 cut(s) 294, 768
EciI GGCGGA 2 cut(s) 737, 1227
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 4 cut(s) 998, 1820, 2178, 2400
Eco24I GRGCYC 2 cut(s) 717, 2265
Eco31I GGTCTC 2 cut(s) 1277, 1434
Eco47I GGWCC 1 cut(s) 2236
Eco53kI GAGCTC 1 cut(s) 715
Eco91I GGTNACC 1 cut(s) 1243
EcoICRI GAGCTC 1 cut(s) 715
EcoO109I RGGNCCY 1 cut(s) 2236
EcoO65I GGTNACC 1 cut(s) 1243
EcoRII CCWGG 3 cut(s) 801, 1831, 2148
EcoT14I CCWWGG 4 cut(s) 998, 1820, 2178, 2400
EcoT22I ATGCAT 3 cut(s) 437, 812, 1359
EcoT38I GRGCYC 2 cut(s) 717, 2265
EgeI GGCGCC 1 cut(s) 1315
EheI GGCGCC 1 cut(s) 1315
ErhI CCWWGG 4 cut(s) 998, 1820, 2178, 2400
FalI AAGNNNNNCTT 4 cut(s) 62, 94, 1931, 1963
FaqI GGGAC 5 cut(s) 551, 893, 1334, 2054, 2191
FauI CCCGC 2 cut(s) 650, 1919
FbaI TGATCA 1 cut(s) 1426
FblI GTMKAC 2 cut(s) 375, 1121
FokI GGATG 5 cut(s) 47, 319, 333, 774, 1315
FriOI GRGCYC 2 cut(s) 717, 2265
FspBI CTAG 4 cut(s) 230, 999, 1793, 2108
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 5 cut(s) 603, 1315, 1339, 1842, 2406
GsuI CTGGAG 2 cut(s) 853, 1854
HaeII RGCGCY 2 cut(s) 1317, 1341
HaeIII GGCC 4 cut(s) 207, 1411, 1979, 2177
HapII CCGG 8 cut(s) 534, 1047, 1241, 1980, 2073, 2209, 2313, 2360
HhaI GCGC 5 cut(s) 604, 1316, 1340, 1843, 2407
Hin1I GRCGYC 1 cut(s) 1314
Hin6I GCGC 5 cut(s) 602, 1314, 1338, 1841, 2405
HinP1I GCGC 5 cut(s) 602, 1314, 1338, 1841, 2405
HindIII AAGCTT 3 cut(s) 1079, 1487, 2249
HinfI GANTC 8 cut(s) 23, 123, 385, 675, 778, 1234, 1450, 2009
HpaII CCGG 8 cut(s) 534, 1047, 1241, 1980, 2073, 2209, 2313, 2360
HphI GGTGA 5 cut(s) 154, 518, 1237, 1609, 1942
Hpy166II GTNNAC 6 cut(s) 376, 1122, 1459, 1558, 1711, 2365
Hpy188III TCNNGA 8 cut(s) 70, 782, 791, 832, 980, 1180, 1738, 1769
Hpy8I GTNNAC 6 cut(s) 376, 1122, 1459, 1558, 1711, 2365
Hpy99I CGWCG 1 cut(s) 2024
HpyCH4III ACNGT 5 cut(s) 506, 698, 2044, 2068, 2274
HpyCH4IV ACGT 6 cut(s) 189, 906, 2204, 2267, 2390, 2421
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 441, 669, 1849, 2036, 2045, 2248
HpyF3I CTNAG 7 cut(s) 668, 1017, 1114, 1192, 1371, 1476, 1522
HpySE526I ACGT 6 cut(s) 189, 906, 2204, 2267, 2390, 2421
Hsp92I GRCGYC 1 cut(s) 1314
HspAI GCGC 5 cut(s) 602, 1314, 1338, 1841, 2405
KasI GGCGCC 1 cut(s) 1313
KpnI GGTACC 2 cut(s) 2215, 2456
Ksp22I TGATCA 1 cut(s) 1426
LmnI GCTCC 6 cut(s) 99, 253, 660, 724, 1835, 2021
Lsp1109I GCAGC 8 cut(s) 388, 1387, 1570, 1808, 1825, 1830, 2014, 2338
LweI GCATC 4 cut(s) 412, 819, 1366, 2088
MaeI CTAG 4 cut(s) 230, 999, 1793, 2108
MaeII ACGT 6 cut(s) 189, 906, 2204, 2267, 2390, 2421
MaeIII GTNAC 9 cut(s) 173, 358, 850, 1243, 1597, 2170, 2268, 2386, 2465
MflI RGATCY 3 cut(s) 580, 976, 1617
MhlI GDGCHC 2 cut(s) 717, 2265
MlsI TGGCCA 1 cut(s) 207
MluI ACGCGT 1 cut(s) 1212
MluNI TGGCCA 1 cut(s) 207
Mly113I GGCGCC 1 cut(s) 1314
MlyI GAGTC 2 cut(s) 379, 2018
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 3 cut(s) 437, 812, 1359
MroXI GAANNNNTTC 2 cut(s) 1629, 2225
MscI TGGCCA 1 cut(s) 207
MseI TTAA 8 cut(s) 222, 369, 588, 756, 2201, 2331, 2445, 2485
MslI CAYNNNNRTG 3 cut(s) 573, 2177, 2183
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 2461
MspI CCGG 8 cut(s) 534, 1047, 1241, 1980, 2073, 2209, 2313, 2360
MspR9I CCNGG 7 cut(s) 535, 803, 1047, 1833, 2073, 2150, 2209
MvaI CCWGG 3 cut(s) 803, 1833, 2150
MvnI CGCG 2 cut(s) 299, 1214
MwoI GCNNNNNNNGC 7 cut(s) 152, 441, 669, 1849, 2036, 2045, 2248
NarI GGCGCC 1 cut(s) 1314
NciI CCSGG 4 cut(s) 535, 1047, 2073, 2209
NcoI CCATGG 2 cut(s) 2178, 2400
NlaIV GGNNCC 4 cut(s) 662, 1315, 2213, 2454
NmuCI GTSAC 6 cut(s) 173, 358, 1243, 1597, 2170, 2268
NsbI TGCGCA 1 cut(s) 603
NsiI ATGCAT 3 cut(s) 437, 812, 1359
NspI RCATGY 3 cut(s) 221, 439, 810
PaeI GCATGC 2 cut(s) 439, 810
PagI TCATGA 2 cut(s) 1179, 1768
PaqCI CACCTGC 2 cut(s) 1070, 1478
PciI ACATGT 1 cut(s) 217
PdmI GAANNNNTTC 2 cut(s) 1629, 2225
PfeI GAWTC 6 cut(s) 23, 123, 675, 778, 1234, 1450
PflMI CCANNNNNTGG 2 cut(s) 628, 1385
PfoI TCCNGGA 2 cut(s) 1045, 1831
PleI GAGTC 2 cut(s) 379, 2017
PluTI GGCGCC 1 cut(s) 1317
PpsI GAGTC 2 cut(s) 379, 2017
Ppu21I YACGTR 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 2236
PscI ACATGT 1 cut(s) 217
PsiI TTATAA 1 cut(s) 1296
Psp124BI GAGCTC 1 cut(s) 717
Psp1406I AACGTT 1 cut(s) 2390
Psp5II RGGWCCY 1 cut(s) 2236
Psp6I CCWGG 3 cut(s) 801, 1831, 2148
PspEI GGTNACC 1 cut(s) 1243
PspGI CCWGG 3 cut(s) 801, 1831, 2148
PspN4I GGNNCC 4 cut(s) 662, 1315, 2213, 2454
PspPI GGNCC 2 cut(s) 1409, 2236
PspPPI RGGWCCY 1 cut(s) 2236
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 3 cut(s) 580, 976, 1617
RsaI GTAC 7 cut(s) 5, 928, 1211, 1712, 2213, 2420, 2454
RsaNI GTAC 7 cut(s) 4, 927, 1210, 1711, 2212, 2419, 2453
RseI CAYNNNNRTG 3 cut(s) 573, 2177, 2183
SacI GAGCTC 1 cut(s) 717
SaqAI TTAA 8 cut(s) 222, 369, 588, 756, 2201, 2331, 2445, 2485
Sau96I GGNCC 2 cut(s) 1409, 2236
SchI GAGTC 2 cut(s) 379, 2018
ScrFI CCNGG 7 cut(s) 535, 803, 1047, 1833, 2073, 2150, 2209
SduI GDGCHC 2 cut(s) 717, 2265
SfaNI GCATC 4 cut(s) 412, 819, 1366, 2088
SfcI CTRYAG 2 cut(s) 127, 990
SfoI GGCGCC 1 cut(s) 1315
SinI GGWCC 1 cut(s) 2236
SmiMI CAYNNNNRTG 3 cut(s) 573, 2177, 2183
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SphI GCATGC 2 cut(s) 439, 810
SspDI GGCGCC 1 cut(s) 1313
SspMI CTAG 4 cut(s) 230, 999, 1793, 2108
SstI GAGCTC 1 cut(s) 717
StyD4I CCNGG 7 cut(s) 533, 801, 1045, 1831, 2071, 2148, 2207
StyI CCWWGG 4 cut(s) 998, 1820, 2178, 2400
TaaI ACNGT 5 cut(s) 506, 698, 2044, 2068, 2274
TaiI ACGT 6 cut(s) 192, 909, 2207, 2270, 2393, 2424
TaqI TCGA 3 cut(s) 948, 1004, 2127
TaqII GACCGA 1 cut(s) 1199
TatI WGTACW 1 cut(s) 1710
TauI GCSGC 3 cut(s) 302, 1314, 2079
TfiI GAWTC 6 cut(s) 23, 123, 675, 778, 1234, 1450
Tru1I TTAA 8 cut(s) 222, 369, 588, 756, 2201, 2331, 2445, 2485
Tru9I TTAA 8 cut(s) 222, 369, 588, 756, 2201, 2331, 2445, 2485
TscAI CASTG 4 cut(s) 701, 1101, 1641, 2277
TseFI GTSAC 6 cut(s) 173, 358, 1243, 1597, 2170, 2268
TseI GCWGC 8 cut(s) 401, 1375, 1583, 1796, 1838, 1843, 2027, 2351
Tsp45I GTSAC 6 cut(s) 173, 358, 1243, 1597, 2170, 2268
TspDTI ATGAA 5 cut(s) 591, 808, 1233, 1667, 1785
TspGWI ACGGA 6 cut(s) 47, 1139, 1550, 1577, 2008, 2158
TspRI CASTG 4 cut(s) 701, 1101, 1641, 2277
Van91I CCANNNNNTGG 2 cut(s) 628, 1385
VpaK11BI GGWCC 1 cut(s) 2236
XapI RAATTY 1 cut(s) 261
XceI RCATGY 3 cut(s) 221, 439, 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 2146
XmaJI CCTAGG 1 cut(s) 998
XmiI GTMKAC 2 cut(s) 375, 1121
XmnI GAANNNNTTC 2 cut(s) 1629, 2225
XspI CTAG 4 cut(s) 230, 999, 1793, 2108
Zsp2I ATGCAT 3 cut(s) 437, 812, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.