MD03G1258800.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000723
Physical Location & Seq
Reverse (-)
34598548 .. 34600281
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1258800.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGGGAAGAGGGAAACACCTTTGCTGATTGCTGCAACAAATGGTGTGACTGAAATGGTGGGGAAGATACTTGAAAATTTTCCCGTGGCCATCCGGGACACTAACTCAGAGAAAAAGAATGTGGTATTGTTAGCTGCACAGAACAGGCAACTCCATGTGTACCAGCTCTTGCTAAACAAAAAAACCCCGATCAGAGACAATGTGTTCAGCGAAGTGGATAATGCGGGGAATAGTGTGTTACATCTCGCAGCAACAGCAATGTTAGAAGCCAATAACCCTTGCATTAATCTTGGGCCTGCGTTACAAATGCAAAGGGAAATCAAATGGTTTGAGTTCGTAAAAAGCTCCATGCCACCACACTTCTTTGCCCCCTACAACAAGAAAAACCAGACTGCAAGGGCTATATTCAGTGAATCACACACTGAGCTAGTCAAAACCGGAGGAGAATGGCTCACAAAGACCTCTGAGAACTGCTCTGAGAACTGCTCGGTCATGGGTGGCCTTATCGCCACTGTCGCCTTTGCCACTGCTACTGCAGTTCCTGGGGGCATCAGAGAGGCTACTGGCAGTCCAATCCTTCAAAACCAGGCAGCTTTTGAGGTGTTTTCCATCTTATCTCTCTTTGCGCTGTGCTCCTCCGTCACATCCATGGCCATATTCCTGAGCATCCTAATGTCCCGGTTCCCCGAAAGGGCTTTTGGAAAGGCGCTGCCTAGCAAGTTTGTAGTGGGCTTAACAATGCTCTTCATGTCCTTGCTTTCGATGCTGGTTTCATTTTGCGCTGGACATTTCTTCATGCTAAAGGATAAGCTCAAACACTTTGCCGTGCCTGTTTATGGTGAAGGGGATGAGGATGAAGATGTTGACAAGAATGATACAGAAAAGCACAATGGAGAACGGAGCAAAGTGCTAGCCAATGCCAAGGAATTGGGAGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0000166 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006915 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0006996 GO:0007154 GO:0007165 GO:0007166 GO:0008144 GO:0008150 GO:0008152 GO:0008219 GO:0008625 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010469 GO:0010506 GO:0010508 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0010941 GO:0010942 GO:0010950 GO:0010952 GO:0012501 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017075 GO:0017076 GO:0017148 GO:0019222 GO:0019538 GO:0019905 GO:0022607 GO:0022898 GO:0023051 GO:0023052 GO:0023057 GO:0030162 GO:0030554 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032270 GO:0032409 GO:0032412 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0033194 GO:0033554 GO:0034097 GO:0034248 GO:0034249 GO:0034341 GO:0034599 GO:0034762 GO:0034765 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0042221 GO:0042802 GO:0042981 GO:0043065 GO:0043066 GO:0043067 GO:0043068 GO:0043069 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043269 GO:0043280 GO:0043281 GO:0043412 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045862 GO:0046777 GO:0046907 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051248 GO:0051336 GO:0051345 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0061024 GO:0065003 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071345 GO:0071346 GO:0071447 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090114 GO:0097159 GO:0097190 GO:0097191 GO:0097367 GO:0099601 GO:0140096 GO:1900449 GO:1901265 GO:1901363 GO:1901564 GO:1901700 GO:1901701 GO:1902041 GO:1902042 GO:1904062 GO:2000112 GO:2000113 GO:2000116 GO:2000310 GO:2001056 GO:2001233 GO:2001234 GO:2001236 GO:2001237 GO:2001257
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

314

Amino Acids

34.35

Weight (kDa)

7.05

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 155 - 265 7.3e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 224
AcoI YGGCCR 2 cut(s) 87, 651
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 691, 836
AgsI TTSAA 2 cut(s) 74, 581
AjnI CCWGG 2 cut(s) 541, 585
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 6 cut(s) 134, 166, 345, 427, 593, 811
AluI AGCT 6 cut(s) 134, 166, 345, 427, 593, 811
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 1 cut(s) 189
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 4 cut(s) 87, 293, 499, 651
ApeKI GCWGC 5 cut(s) 32, 134, 248, 590, 709
ApoI RAATTY 1 cut(s) 76
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 3 cut(s) 628, 709, 782
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 2 cut(s) 95, 679
AsuHPI GGTGA 1 cut(s) 851
AsuNHI GCTAGC 1 cut(s) 910
BalI TGGCCA 2 cut(s) 89, 653
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 5 cut(s) 19, 121, 260, 602, 696
BccI CCATC 2 cut(s) 98, 617
BceAI ACGGC 1 cut(s) 809
BciT130I CCWGG 2 cut(s) 543, 587
BcnI CCSGG 2 cut(s) 95, 679
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 3 cut(s) 428, 714, 911
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 710
BisI GCNGC 5 cut(s) 33, 135, 249, 591, 710
BlsI GCNGC 5 cut(s) 34, 136, 250, 592, 711
Bme1390I CCNGG 4 cut(s) 95, 543, 587, 679
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 683
BmrFI CCNGG 4 cut(s) 95, 543, 587, 679
BmsI GCATC 3 cut(s) 558, 675, 753
BmtI GCTAGC 1 cut(s) 914
BplI GAGNNNNNCTC 6 cut(s) 435, 467, 458, 490, 470, 502
Bpu10I CCTNAGC 1 cut(s) 662
BpuMI CCSGG 2 cut(s) 95, 679
BsaJI CCNNGG 4 cut(s) 84, 542, 648, 921
BsaWI WCCGGW 1 cut(s) 437
Bsc4I CCNNNNNNNGG 2 cut(s) 691, 836
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 543, 587
BseDI CCNNGG 4 cut(s) 84, 542, 648, 921
BseGI GGATG 5 cut(s) 90, 644, 666, 853, 859
BseLI CCNNNNNNNGG 2 cut(s) 691, 836
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 5 cut(s) 120, 414, 456, 468, 653
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 2 cut(s) 456, 625
BseXI GCAGC 5 cut(s) 19, 121, 260, 602, 696
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 4 cut(s) 89, 295, 501, 653
BsiHKAI GWGCWC 1 cut(s) 635
BsiSI CCGG 3 cut(s) 94, 438, 679
BslFI GGGAC 2 cut(s) 110, 661
BslI CCNNNNNNNGG 2 cut(s) 691, 836
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 110, 661
BsnI GGCC 4 cut(s) 89, 295, 501, 653
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 1 cut(s) 189
Bsp19I CCATGG 1 cut(s) 648
BspACI CCGC 1 cut(s) 224
BspANI GGCC 4 cut(s) 89, 295, 501, 653
BspCNI CTCAG 5 cut(s) 119, 415, 457, 469, 654
BspLI GGNNCC 1 cut(s) 683
BspMAI CTGCAG 1 cut(s) 538
BspOI GCTAGC 1 cut(s) 914
BspQI GCTCTTC 1 cut(s) 749
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 4 cut(s) 84, 542, 648, 921
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 648, 921
Bst2UI CCWGG 2 cut(s) 543, 587
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 2 cut(s) 2, 749
BstC8I GCNNGC 2 cut(s) 297, 912
BstDEI CTNAG 5 cut(s) 106, 423, 465, 477, 662
BstDSI CCRYGG 2 cut(s) 84, 648
BstF5I GGATG 5 cut(s) 90, 644, 666, 853, 859
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 3 cut(s) 628, 709, 782
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 3 cut(s) 254, 515, 763
BstNI CCWGG 2 cut(s) 543, 587
BstSCI CCNGG 4 cut(s) 93, 541, 585, 677
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 5 cut(s) 19, 121, 260, 602, 696
BstXI CCANNNNNNTGG 1 cut(s) 928
BsuRI GGCC 4 cut(s) 89, 295, 501, 653
BtgI CCRYGG 2 cut(s) 84, 648
BtsCI GGATG 5 cut(s) 90, 644, 666, 853, 859
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 4 cut(s) 415, 420, 510, 525
Cac8I GCNNGC 2 cut(s) 297, 912
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 3 cut(s) 628, 709, 782
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 1 cut(s) 160
CviAII CATG 6 cut(s) 155, 349, 493, 649, 748, 796
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 5 cut(s) 106, 423, 465, 477, 662
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EaeI YGGCCR 2 cut(s) 87, 651
Eam1104I CTCTTC 2 cut(s) 2, 749
EarI CTCTTC 2 cut(s) 2, 749
Eco130I CCWWGG 2 cut(s) 648, 921
EcoRII CCWGG 2 cut(s) 541, 585
EcoT14I CCWWGG 2 cut(s) 648, 921
ErhI CCWWGG 2 cut(s) 648, 921
FaeI CATG 6 cut(s) 158, 352, 496, 652, 751, 799
FaiI YATR 9 cut(s) 156, 350, 404, 494, 650, 656, 749, 797, 837
FaqI GGGAC 2 cut(s) 110, 661
FatI CATG 6 cut(s) 154, 348, 492, 648, 747, 795
FauI CCCGC 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 33, 135, 249, 591, 710
FokI GGATG 5 cut(s) 77, 631, 653, 860, 866
Fsp4HI GCNGC 5 cut(s) 33, 135, 249, 591, 710
FspBI CTAG 3 cut(s) 428, 714, 911
GlaI GCGC 3 cut(s) 627, 708, 781
GluI GCNGC 5 cut(s) 33, 135, 249, 591, 710
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 4 cut(s) 89, 295, 501, 653
HapII CCGG 3 cut(s) 94, 438, 679
HhaI GCGC 3 cut(s) 628, 709, 782
Hin1II CATG 6 cut(s) 158, 352, 496, 652, 751, 799
Hin6I GCGC 3 cut(s) 626, 707, 780
HinP1I GCGC 3 cut(s) 626, 707, 780
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HinfI GANTC 1 cut(s) 413
HpaII CCGG 3 cut(s) 94, 438, 679
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 2 cut(s) 160, 865
Hpy188I TCNGA 5 cut(s) 109, 194, 466, 478, 554
Hpy188III TCNNGA 1 cut(s) 661
Hpy8I GTNNAC 2 cut(s) 160, 865
HpyAV CCTTC 2 cut(s) 587, 836
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4V TGCA 6 cut(s) 35, 137, 282, 310, 395, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 515, 763
HpyF3I CTNAG 5 cut(s) 106, 423, 465, 477, 662
Hsp92II CATG 6 cut(s) 158, 352, 496, 652, 751, 799
HspAI GCGC 3 cut(s) 626, 707, 780
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 749
LmnI GCTCC 3 cut(s) 350, 638, 900
Lsp1109I GCAGC 5 cut(s) 19, 121, 260, 602, 696
LweI GCATC 3 cut(s) 558, 675, 753
MaeI CTAG 3 cut(s) 428, 714, 911
MaeIII GTNAC 4 cut(s) 46, 237, 300, 640
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 5 cut(s) 19, 76, 736, 784, 869
MhlI GDGCHC 1 cut(s) 635
MlsI TGGCCA 2 cut(s) 89, 653
MluCI AATT 2 cut(s) 76, 926
MluNI TGGCCA 2 cut(s) 89, 653
MnlI CCTC 7 cut(s) 3, 434, 472, 550, 592, 646, 844
Mox20I TGGCCA 2 cut(s) 89, 653
MroXI GAANNNNTTC 1 cut(s) 78
MscI TGGCCA 2 cut(s) 89, 653
MseI TTAA 2 cut(s) 285, 734
MslI CAYNNNNRTG 2 cut(s) 647, 671
Msp20I TGGCCA 2 cut(s) 89, 653
MspI CCGG 3 cut(s) 94, 438, 679
MspR9I CCNGG 4 cut(s) 95, 543, 587, 679
MvaI CCWGG 2 cut(s) 543, 587
MwoI GCNNNNNNNGC 3 cut(s) 254, 515, 763
NciI CCSGG 2 cut(s) 95, 679
NcoI CCATGG 1 cut(s) 648
NdeII GATC 1 cut(s) 189
NheI GCTAGC 1 cut(s) 910
NlaIII CATG 6 cut(s) 158, 352, 496, 652, 751, 799
NlaIV GGNNCC 1 cut(s) 683
NmuCI GTSAC 2 cut(s) 46, 640
PciSI GCTCTTC 1 cut(s) 749
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 5 cut(s) 34, 136, 250, 592, 711
PshBI ATTAAT 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 541, 585
PspGI CCWGG 2 cut(s) 541, 585
PspN4I GGNNCC 1 cut(s) 683
PspPI GGNCC 1 cut(s) 293
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 542
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 2 cut(s) 647, 671
SapI GCTCTTC 1 cut(s) 749
SaqAI TTAA 2 cut(s) 285, 734
SatI GCNGC 5 cut(s) 33, 135, 249, 591, 710
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 293
ScrFI CCNGG 4 cut(s) 95, 543, 587, 679
SduI GDGCHC 1 cut(s) 635
SetI ASST 9 cut(s) 22, 136, 168, 347, 429, 464, 595, 603, 813
SfaNI GCATC 3 cut(s) 558, 675, 753
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 647, 671
Sse9I AATT 2 cut(s) 76, 926
SsiI CCGC 1 cut(s) 224
SspMI CTAG 3 cut(s) 428, 714, 911
StyD4I CCNGG 4 cut(s) 93, 541, 585, 677
StyI CCWWGG 2 cut(s) 648, 921
TaaI ACNGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 761
TaqII GACCGA 1 cut(s) 478
TasI AATT 2 cut(s) 76, 926
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 285, 734
Tru9I TTAA 2 cut(s) 285, 734
TscAI CASTG 4 cut(s) 415, 427, 517, 532
TseFI GTSAC 2 cut(s) 46, 640
TseI GCWGC 5 cut(s) 32, 134, 248, 590, 709
Tsp45I GTSAC 2 cut(s) 46, 640
TspDTI ATGAA 4 cut(s) 736, 762, 784, 870
TspGWI ACGGA 2 cut(s) 628, 913
TspRI CASTG 4 cut(s) 415, 427, 517, 532
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 3 cut(s) 428, 714, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.