FvH4_2g24480

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002400
Physical Location & Seq
Reverse (-)
19960840 .. 19965017
4178 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24480.t1

Sequence Viewer

Length: 1347 bp
ATGAAAGTGAAAGTGCGTGGAACCCTGAAAGAAGCGAGACCGAGAGCCAGGGTTGACATAGCCAGGGCTGAGAGAGCTAGAGGCAAAAGAGCTAGAGCCGACATAAGAGCTAGAGCCGACATAACCAGGCCTGAGAGAGGTTTGTTTTCTCCAAAATTGAAGAGGCCGAGAGTGGATGATTTTGGAAATTTTTCCAGAAGGCATGGATCATTGCAAACTAGTGAACATGAAGATCACAATTCTCTACTTGATCGTAATTTGAGGTCGGACAAAGCTAGAGATGACAGATTCAGGGCAGAGAGATCTAGGGATGGCCGATTCAGGGCAGAGCGAGCCAGACCCGACAGAGGCTGGGCAGAGCGAGCTAGATCCGACAGAGCCAGAGCCCACGTAGCCAGGGTTGAGAGAACCCGAGCCTACAGAGCTGGGGCTGACAGAATTTTATTTACTCCAAAGTTGAAGAGGCCAAAGCCAAGAGTGGAAAGTCATGGATCAATGCAAGGTAATGAACGTGAAGATTATTATCGACTTGATTGTGATTTGGGTAAATCATCAAATCTCCAGCCGGATAAGATTTCGCAATTTTCTGTCCAACATGTTGGAATTGTCAAAACTACAAGAGGAGAAAGTTTTCCCTCACCAAGTATAGTGAAAGAAAACTCAGTAAAGAGTAAAGAGGATGAGAATACTAGCCGCCAAGGACTCATGATGCGACAACCTTTGTCTGATTATTATCACTCTGATATGACGGTTACATGTGGAACTTTTGATGTTAGTCAGCAACATGTGTCATGTTTGGAAGTTAAAGATCTTAGCGAGGTGAAAAGGGATCTTGAAGTACTCGAGGATACTTCAGGAAAATCCAGGAAACAAAATCGGCACAAAACCAACATAGTCTCTAGCAATGTTGAACATACAGATCGTACCAGGGTCAAAGGGGCCTTAGAAGCTTATAAGGAGATATTGATATTGACCAAGTCATTGACCAAGTGGAGGGCACAAAACTGTAATATTTATGTTCAAGCAGCAATGGATATGAAAAAACAAGGAAAATGGGTAAACACGATGAAACAAATGGGATCTATTGGAGGAGTAAGAGTTGGTGATAAGTTTCGTTATAGAGCTCAACTAAACATTATTGGTCTTCACAAACAATTTAACGATGGTATTGATCGTATGAGGAGAAATGACGGTAAAATTTTGGCCATAAGTATTGTTGATTCTGGTCGTTATGCCAACAATCTGCAATCTTCTGATATTTTGATCTATTCCGGTCATGGTGGAAAGGCTCTGTTTGATAAAACGAAACCGACAGATCAAATAATGCAAGGTGAAACCTTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

449

Amino Acids

51.37

Weight (kDa)

10.23

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 359 - 445 2.3e-18 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
AciI CCGC 1 cut(s) 694
AclWI GGATC 5 cut(s) 214, 363, 499, 837, 1087
AcoI YGGCCR 2 cut(s) 313, 1203
AcsI RAATTY 3 cut(s) 187, 438, 1197
AcuI CTGAAG 1 cut(s) 837
AfaI GTAC 2 cut(s) 840, 925
AfiI CCNNNNNNNGG 4 cut(s) 137, 322, 347, 993
AflIII ACRYGT 3 cut(s) 595, 755, 784
AgsI TTSAA 5 cut(s) 160, 460, 836, 911, 1022
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 6 cut(s) 47, 62, 125, 395, 863, 926
AluBI AGCT 8 cut(s) 77, 92, 110, 275, 365, 425, 950, 1124
AluI AGCT 8 cut(s) 77, 92, 110, 275, 365, 425, 950, 1124
Alw21I GWGCWC 1 cut(s) 1126
Alw26I GTCTC 2 cut(s) 31, 901
AlwI GGATC 5 cut(s) 214, 363, 499, 837, 1087
AlwNI CAGNNNCTG 1 cut(s) 351
Ama87I CYCGRG 2 cut(s) 411, 842
AoxI GGCC 6 cut(s) 128, 164, 313, 464, 939, 1203
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 3 cut(s) 187, 438, 1197
Asp700I GAANNNNTTC 2 cut(s) 190, 630
AspS9I GGNCC 1 cut(s) 939
AsuHPI GGTGA 4 cut(s) 630, 832, 1115, 1343
AvaI CYCGRG 2 cut(s) 411, 842
BaeGI GKGCMC 1 cut(s) 1000
BalI TGGCCA 1 cut(s) 1205
BanII GRGCYC 2 cut(s) 388, 1126
BbsI GAAGAC 1 cut(s) 1136
Bbv12I GWGCWC 1 cut(s) 1126
BbvI GCAGC 1 cut(s) 1037
BccI CCATC 2 cut(s) 305, 1157
BciT130I CCWGG 6 cut(s) 49, 64, 127, 397, 865, 928
BciVI GTATCC 1 cut(s) 841
BcoDI GTCTC 2 cut(s) 31, 901
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 9 cut(s) 78, 93, 111, 219, 276, 306, 366, 690, 900
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 841
BglII AGATCT 2 cut(s) 302, 808
BisI GCNGC 2 cut(s) 694, 1026
BlsI GCNGC 2 cut(s) 695, 1027
BmcAI AGTACT 1 cut(s) 840
Bme1390I CCNGG 6 cut(s) 49, 64, 127, 397, 865, 928
BmeT110I CYCGRG 2 cut(s) 411, 842
BmgT120I GGNCC 1 cut(s) 939
BmiI GGNNCC 2 cut(s) 22, 940
BmrFI CCNGG 6 cut(s) 49, 64, 127, 397, 865, 928
BmsI GCATC 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 1136
BpmI CTGGAG 1 cut(s) 545
BsaAI YACGTR 1 cut(s) 391
BsaBI GATNNNNATC 2 cut(s) 522, 732
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 5 cut(s) 48, 63, 396, 697, 927
BsaWI WCCGGW 1 cut(s) 1271
BsaXI ACNNNNNCTCC 2 cut(s) 1083, 1113
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 322, 347, 993
Bse3DI GCAATG 3 cut(s) 209, 910, 1035
Bse8I GATNNNNATC 2 cut(s) 522, 732
BseBI CCWGG 6 cut(s) 49, 64, 127, 397, 865, 928
BseDI CCNNGG 5 cut(s) 48, 63, 396, 697, 927
BseGI GGATG 3 cut(s) 181, 316, 685
BseJI GATNNNNATC 2 cut(s) 522, 732
BseLI CCNNNNNNNGG 4 cut(s) 137, 322, 347, 993
BseMI GCAATG 3 cut(s) 209, 910, 1035
BseMII CTCAG 3 cut(s) 60, 123, 675
BseRI GAGGAG 3 cut(s) 636, 1104, 1195
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 1 cut(s) 1037
BseYI CCCAGC 2 cut(s) 351, 425
BshFI GGCC 6 cut(s) 130, 166, 315, 466, 941, 1205
BsiHKAI GWGCWC 1 cut(s) 1126
BsiHKCI CYCGRG 2 cut(s) 411, 842
BsiSI CCGG 2 cut(s) 566, 1272
BslI CCNNNNNNNGG 4 cut(s) 137, 322, 347, 993
BsmAI GTCTC 2 cut(s) 31, 901
BsnI GGCC 6 cut(s) 130, 166, 315, 466, 941, 1205
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 2 cut(s) 411, 842
Bsp1286I GDGCHC 3 cut(s) 388, 1000, 1126
BspACI CCGC 1 cut(s) 694
BspANI GGCC 6 cut(s) 130, 166, 315, 466, 941, 1205
BspCNI CTCAG 3 cut(s) 61, 124, 674
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 2 cut(s) 22, 940
BspPI GGATC 5 cut(s) 214, 363, 499, 837, 1087
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 209, 910, 1035
BssECI CCNNGG 5 cut(s) 48, 63, 396, 697, 927
BssT1I CCWWGG 1 cut(s) 697
Bst2UI CCWGG 6 cut(s) 49, 64, 127, 397, 865, 928
Bst4CI ACNGT 3 cut(s) 751, 1007, 1193
Bst6I CTCTTC 2 cut(s) 155, 455
BstBAI YACGTR 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 333, 363
BstDEI CTNAG 5 cut(s) 69, 132, 661, 812, 943
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 181, 316, 685
BstMAI GTCTC 2 cut(s) 31, 901
BstMWI GCNNNNNNNGC 6 cut(s) 74, 332, 362, 392, 422, 947
BstNI CCWGG 6 cut(s) 49, 64, 127, 397, 865, 928
BstNSI RCATGY 3 cut(s) 599, 759, 788
BstSCI CCNGG 6 cut(s) 47, 62, 125, 395, 863, 926
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 1000
BstV1I GCAGC 1 cut(s) 1037
BstV2I GAAGAC 1 cut(s) 1136
BstX2I RGATCY 5 cut(s) 302, 368, 808, 829, 1079
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 5 cut(s) 302, 368, 808, 829, 1079
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 6 cut(s) 130, 166, 315, 466, 941, 1205
BtsCI GGATG 3 cut(s) 181, 316, 685
Cac8I GCNNGC 2 cut(s) 333, 363
CaiI CAGNNNCTG 1 cut(s) 351
CciI TCATGA 1 cut(s) 705
Cfr13I GGNCC 1 cut(s) 939
Csp6I GTAC 2 cut(s) 839, 924
CviAII CATG 9 cut(s) 203, 227, 488, 596, 706, 756, 785, 792, 1277
CviQI GTAC 2 cut(s) 839, 924
DdeI CTNAG 5 cut(s) 69, 132, 661, 812, 943
EaeI YGGCCR 2 cut(s) 313, 1203
Eam1104I CTCTTC 2 cut(s) 155, 455
EarI CTCTTC 2 cut(s) 155, 455
Ecl136II GAGCTC 1 cut(s) 1124
Eco130I CCWWGG 1 cut(s) 697
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 388, 1126
Eco31I GGTCTC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 1124
Eco57I CTGAAG 1 cut(s) 837
Eco88I CYCGRG 2 cut(s) 411, 842
EcoICRI GAGCTC 1 cut(s) 1124
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 939
EcoRII CCWGG 6 cut(s) 47, 62, 125, 395, 863, 926
EcoT14I CCWWGG 1 cut(s) 697
EcoT38I GRGCYC 2 cut(s) 388, 1126
ErhI CCWWGG 1 cut(s) 697
FaeI CATG 9 cut(s) 206, 230, 491, 599, 709, 759, 788, 795, 1280
FatI CATG 9 cut(s) 202, 226, 487, 595, 705, 755, 784, 791, 1276
Fnu4HI GCNGC 2 cut(s) 694, 1026
FokI GGATG 3 cut(s) 188, 323, 692
FriOI GRGCYC 2 cut(s) 388, 1126
Fsp4HI GCNGC 2 cut(s) 694, 1026
FspBI CTAG 9 cut(s) 78, 93, 111, 219, 276, 306, 366, 690, 900
GluI GCNGC 2 cut(s) 694, 1026
GsaI CCCAGC 2 cut(s) 355, 429
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 6 cut(s) 130, 166, 315, 466, 941, 1205
HapII CCGG 2 cut(s) 566, 1272
Hin1II CATG 9 cut(s) 206, 230, 491, 599, 709, 759, 788, 795, 1280
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 948
HinfI GANTC 4 cut(s) 288, 318, 702, 1220
HpaII CCGG 2 cut(s) 566, 1272
HphI GGTGA 4 cut(s) 630, 832, 1115, 1343
Hpy166II GTNNAC 3 cut(s) 55, 224, 1060
Hpy188I TCNGA 5 cut(s) 268, 373, 727, 742, 1255
Hpy188III TCNNGA 4 cut(s) 195, 706, 833, 855
Hpy8I GTNNAC 3 cut(s) 55, 224, 1060
HpyAV CCTTC 1 cut(s) 192
HpyCH4III ACNGT 3 cut(s) 751, 1007, 1193
HpyCH4IV ACGT 2 cut(s) 390, 511
HpyCH4V TGCA 5 cut(s) 214, 499, 1246, 1327, 1342
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 332, 362, 392, 422, 947
HpyF3I CTNAG 5 cut(s) 69, 132, 661, 812, 943
HpySE526I ACGT 2 cut(s) 390, 511
Hsp92II CATG 9 cut(s) 206, 230, 491, 599, 709, 759, 788, 795, 1280
Lsp1109I GCAGC 1 cut(s) 1037
LweI GCATC 1 cut(s) 699
MaeI CTAG 9 cut(s) 78, 93, 111, 219, 276, 306, 366, 690, 900
MaeII ACGT 2 cut(s) 390, 511
MaeIII GTNAC 1 cut(s) 751
MboII GAAGA 6 cut(s) 172, 242, 472, 527, 1136, 1242
MflI RGATCY 5 cut(s) 302, 368, 808, 829, 1079
MhlI GDGCHC 3 cut(s) 388, 1000, 1126
MlsI TGGCCA 1 cut(s) 1205
MluCI AATT 9 cut(s) 155, 187, 238, 256, 438, 581, 603, 1154, 1197
MluNI TGGCCA 1 cut(s) 1205
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 4 cut(s) 246, 396, 580, 616
Mox20I TGGCCA 1 cut(s) 1205
MroXI GAANNNNTTC 2 cut(s) 190, 630
MscI TGGCCA 1 cut(s) 1205
MseI TTAA 2 cut(s) 804, 1158
Msp20I TGGCCA 1 cut(s) 1205
MspI CCGG 2 cut(s) 566, 1272
MspR9I CCNGG 6 cut(s) 49, 64, 127, 397, 865, 928
MvaI CCWGG 6 cut(s) 49, 64, 127, 397, 865, 928
MwoI GCNNNNNNNGC 6 cut(s) 74, 332, 362, 392, 422, 947
NlaIII CATG 9 cut(s) 206, 230, 491, 599, 709, 759, 788, 795, 1280
NlaIV GGNNCC 2 cut(s) 22, 940
NmeAIII GCCGAG 1 cut(s) 192
NspI RCATGY 3 cut(s) 599, 759, 788
PaeR7I CTCGAG 1 cut(s) 842
PagI TCATGA 1 cut(s) 705
PceI AGGCCT 1 cut(s) 130
PciI ACATGT 3 cut(s) 595, 755, 784
PdmI GAANNNNTTC 2 cut(s) 190, 630
PfeI GAWTC 3 cut(s) 288, 318, 1220
PflFI GACNNNGTC 1 cut(s) 976
PfoI TCCNGGA 1 cut(s) 863
PkrI GCNGC 2 cut(s) 695, 1027
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
Ppu21I YACGTR 1 cut(s) 391
PscI ACATGT 3 cut(s) 595, 755, 784
PsiI TTATAA 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 1126
Psp6I CCWGG 6 cut(s) 47, 62, 125, 395, 863, 926
PspFI CCCAGC 2 cut(s) 351, 425
PspGI CCWGG 6 cut(s) 47, 62, 125, 395, 863, 926
PspN4I GGNNCC 2 cut(s) 22, 940
PspPI GGNCC 1 cut(s) 939
PspXI VCTCGAGB 1 cut(s) 842
PstNI CAGNNNCTG 1 cut(s) 351
PsuI RGATCY 5 cut(s) 302, 368, 808, 829, 1079
PsyI GACNNNGTC 1 cut(s) 976
RsaI GTAC 2 cut(s) 840, 925
RsaNI GTAC 2 cut(s) 839, 924
SacI GAGCTC 1 cut(s) 1126
SaqAI TTAA 2 cut(s) 804, 1158
SatI GCNGC 2 cut(s) 694, 1026
Sau96I GGNCC 1 cut(s) 939
ScaI AGTACT 1 cut(s) 840
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 6 cut(s) 49, 64, 127, 397, 865, 928
SduI GDGCHC 3 cut(s) 388, 1000, 1126
SfaNI GCATC 1 cut(s) 699
SfcI CTRYAG 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 842
SlaI CTCGAG 1 cut(s) 842
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 9 cut(s) 155, 187, 238, 256, 438, 581, 603, 1154, 1197
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 1 cut(s) 694
SspI AATATT 1 cut(s) 1012
SspMI CTAG 9 cut(s) 78, 93, 111, 219, 276, 306, 366, 690, 900
SstI GAGCTC 1 cut(s) 1126
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 6 cut(s) 47, 62, 125, 395, 863, 926
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 3 cut(s) 751, 1007, 1193
TaiI ACGT 2 cut(s) 393, 514
TaqI TCGA 2 cut(s) 526, 843
TaqII GACCGA 1 cut(s) 55
TasI AATT 9 cut(s) 155, 187, 238, 256, 438, 581, 603, 1154, 1197
TatI WGTACW 1 cut(s) 838
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 3 cut(s) 288, 318, 1220
Tru1I TTAA 2 cut(s) 804, 1158
Tru9I TTAA 2 cut(s) 804, 1158
TseI GCWGC 1 cut(s) 1025
TspDTI ATGAA 5 cut(s) 17, 243, 522, 1052, 1082
Tth111I GACNNNGTC 1 cut(s) 976
XagI CCTNNNNNAGG 1 cut(s) 135
XapI RAATTY 3 cut(s) 187, 438, 1197
XceI RCATGY 3 cut(s) 599, 759, 788
XhoI CTCGAG 1 cut(s) 842
XmnI GAANNNNTTC 2 cut(s) 190, 630
XspI CTAG 9 cut(s) 78, 93, 111, 219, 276, 306, 366, 690, 900
ZrmI AGTACT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.