Rh1BG350600

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002400
Physical Location & Seq
Reverse (-)
48352716 .. 48354313
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG350600.1

Sequence Viewer

Length: 807 bp
ATGAACACATTACCAAGGAAAAATCGCTCCCTGCCTAGTCCTCGAGTTGATGTTGATGTTGTTCGAGATTTTCCTAAAGGTTGTGGACCGATGGTAGTTTCTTCTTTGAAAGATGCTGAAGAGAAGAGTTTGGTTCCAGACGTTAAAGAATGCGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGTGAAGAAAGCTTTGGCTGCATATCAAGGGACTTTGATTGAGATGGGTGGAGAATGCGATGCTAATTTCAACAGACTAAAGGGTTGGAAATTGTATGTTGAAGCAGCAATGTATCTTAAGAAGAAAGATAAATGGGTTAACACGAAGAAACAAACAGGGGACATTCCAGGAGTGAAGGTTGGTGACAAGTTTCGGTATAGACCTGAATTAGCTATTGTTGGTCTTCATAGTCACTTTCAATGCGGTATTGATTATATGACAGAGGACGGGGAAATGATTGCCACAAGTATTGTTGAATCTGGCCGCTATGCGAACGAGACAGAATCACCTGATGTCAAGATATACACAGGTCAAGGTGGAAATCCACAGGTTTCAAAAGGGGAACCAATAGATCAAAAGCCCGAGCGTGGAAACCGTGCACTGATGAATAGCAAGGCAACAGGAACTCCTGTGAGGGTAATCCGGAGCTATAAATTTCGGGAGGAATCTGGCTATGTTTATCATGGTCTCTACATTGTGGATAATTTTTGGCGAAAAAGAGGAGATTATGGGGAGAGGTATCATGGTGATGAAATATTGTTTATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

268

Amino Acids

30.21

Weight (kDa)

9.04

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 124 - 257 9.9e-41 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 678
AciI CCGC 2 cut(s) 459, 520
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 623
AflII CTTAAG 1 cut(s) 332
AgsI TTSAA 6 cut(s) 109, 286, 317, 455, 512, 591
AjnI CCWGG 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 4 cut(s) 206, 227, 428, 684
AluI AGCT 4 cut(s) 206, 227, 428, 684
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 2 cut(s) 527, 728
Alw44I GTGCAC 1 cut(s) 633
Ama87I CYCGRG 2 cut(s) 42, 617
Aor13HI TCCGGA 1 cut(s) 678
AoxI GGCC 1 cut(s) 517
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 3 cut(s) 157, 233, 320
ApoI RAATTY 1 cut(s) 689
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 4 cut(s) 229, 410, 534, 794
AvaI CYCGRG 2 cut(s) 42, 617
AvaII GGWCC 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 169, 220, 332
BccI CCATC 2 cut(s) 85, 253
BciT130I CCWGG 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 527, 728
BfaI CTAG 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 332
BisI GCNGC 4 cut(s) 158, 234, 321, 520
BlsI GCNGC 4 cut(s) 159, 235, 322, 521
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 2 cut(s) 42, 617
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 135, 600
BmrFI CCNGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 103, 265
BpiI GAAGAC 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 1 cut(s) 14
BsaWI WCCGGW 1 cut(s) 678
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 646, 676
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 678
BseBI CCWGG 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 771
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 3 cut(s) 169, 220, 332
BshFI GGCC 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 2 cut(s) 42, 617
BsiSI CCGG 1 cut(s) 679
BslFI GGGAC 2 cut(s) 259, 389
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 2 cut(s) 527, 728
BsmFI GGGAC 2 cut(s) 259, 389
BsmI GAATGC 2 cut(s) 155, 275
BsnI GGCC 1 cut(s) 519
Bso31I GGTCTC 1 cut(s) 728
BsoBI CYCGRG 2 cut(s) 42, 617
Bsp1286I GDGCHC 1 cut(s) 637
Bsp13I TCCGGA 1 cut(s) 678
Bsp143I GATC 1 cut(s) 607
BspACI CCGC 2 cut(s) 459, 520
BspANI GGCC 1 cut(s) 519
BspEI TCCGGA 1 cut(s) 678
BspLI GGNNCC 2 cut(s) 135, 600
BspTI CTTAAG 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 1 cut(s) 607
BssT1I CCWWGG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 632
Bst6I CTCTTC 2 cut(s) 114, 119
BstAFI CTTAAG 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 155
BstKTI GATC 1 cut(s) 610
BstMAI GTCTC 2 cut(s) 527, 728
BstMBI GATC 1 cut(s) 607
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 384
BstSCI CCNGG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 637
BstV1I GCAGC 3 cut(s) 169, 220, 332
BstV2I GAAGAC 1 cut(s) 431
BsuRI GGCC 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 487, 522
CviAII CATG 2 cut(s) 719, 779
CviJI RGCY 8 cut(s) 206, 227, 233, 428, 519, 616, 684, 708
CviKI_1 RGCY 8 cut(s) 206, 227, 233, 428, 519, 616, 684, 708
DpnI GATC 1 cut(s) 609
DpnII GATC 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 2 cut(s) 114, 119
EarI CTCTTC 2 cut(s) 114, 119
Eco130I CCWWGG 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 728
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 138
Eco88I CYCGRG 2 cut(s) 42, 617
EcoRII CCWGG 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 2 cut(s) 722, 782
FaqI GGGAC 2 cut(s) 259, 389
FatI CATG 2 cut(s) 718, 778
Fnu4HI GCNGC 4 cut(s) 158, 234, 321, 520
Fsp4HI GCNGC 4 cut(s) 158, 234, 321, 520
FspBI CTAG 1 cut(s) 36
GluI GCNGC 4 cut(s) 158, 234, 321, 520
HaeIII GGCC 1 cut(s) 519
HapII CCGG 1 cut(s) 679
Hin1II CATG 2 cut(s) 722, 782
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 3 cut(s) 512, 539, 701
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 1 cut(s) 679
HphI GGTGA 4 cut(s) 229, 410, 534, 794
Hpy166II GTNNAC 3 cut(s) 86, 355, 635
Hpy188I TCNGA 1 cut(s) 806
Hpy188III TCNNGA 5 cut(s) 65, 137, 553, 679, 695
Hpy8I GTNNAC 3 cut(s) 86, 355, 635
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 1 cut(s) 632
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 188, 236, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 2 cut(s) 722, 782
Kpn2I TCCGGA 1 cut(s) 678
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 1 cut(s) 607
LmnI GCTCC 2 cut(s) 32, 681
Lsp1109I GCAGC 3 cut(s) 169, 220, 332
LweI GCATC 2 cut(s) 103, 265
MaeI CTAG 1 cut(s) 36
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 170, 398, 446
MalI GATC 1 cut(s) 609
MboI GATC 1 cut(s) 607
MboII GAAGA 7 cut(s) 93, 131, 136, 232, 349, 373, 431
MhlI GDGCHC 1 cut(s) 637
MluCI AATT 5 cut(s) 280, 305, 422, 689, 739
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 6 cut(s) 51, 472, 663, 691, 749, 765
MroI TCCGGA 1 cut(s) 678
MseI TTAA 3 cut(s) 144, 333, 354
MslI CAYNNNNRTG 1 cut(s) 783
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 1 cut(s) 679
MspR9I CCNGG 1 cut(s) 384
Mva1269I GAATGC 2 cut(s) 155, 275
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 1 cut(s) 607
NlaIII CATG 2 cut(s) 722, 782
NlaIV GGNNCC 2 cut(s) 135, 600
NmuCI GTSAC 3 cut(s) 170, 398, 446
PaeR7I CTCGAG 1 cut(s) 42
PctI GAATGC 2 cut(s) 155, 275
PfeI GAWTC 3 cut(s) 512, 539, 701
PfoI TCCNGGA 1 cut(s) 382
PkrI GCNGC 4 cut(s) 159, 235, 322, 521
Psp6I CCWGG 1 cut(s) 382
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 2 cut(s) 135, 600
PspPI GGNCC 1 cut(s) 86
PspXI VCTCGAGB 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 783
SaqAI TTAA 3 cut(s) 144, 333, 354
SatI GCNGC 4 cut(s) 158, 234, 321, 520
Sau3AI GATC 1 cut(s) 607
Sau96I GGNCC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 2 cut(s) 103, 265
Sfr274I CTCGAG 1 cut(s) 42
SinI GGWCC 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 2 cut(s) 42, 332
SmoI CTYRAG 2 cut(s) 42, 332
Sse9I AATT 5 cut(s) 280, 305, 422, 689, 739
SsiI CCGC 2 cut(s) 459, 520
SspI AATATT 1 cut(s) 792
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 632
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 43, 64
TaqII GACCGA 1 cut(s) 103
TasI AATT 5 cut(s) 280, 305, 422, 689, 739
TauI GCSGC 1 cut(s) 522
TfiI GAWTC 3 cut(s) 512, 539, 701
Tru1I TTAA 3 cut(s) 144, 333, 354
Tru9I TTAA 3 cut(s) 144, 333, 354
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 3 cut(s) 170, 398, 446
TseI GCWGC 3 cut(s) 157, 233, 320
Tsp45I GTSAC 3 cut(s) 170, 398, 446
TspDTI ATGAA 4 cut(s) 17, 431, 656, 801
TspRI CASTG 1 cut(s) 642
Vha464I CTTAAG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 689
XhoI CTCGAG 1 cut(s) 42
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.