Rorug01G0055100

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002400
Physical Location & Seq
Reverse (-)
9041955 .. 9043717
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0055100.1

Sequence Viewer

Length: 1461 bp
ATGAGCTGCGGCGGCTTCACAGTTCCGAAGGGATACCGATTCCATCCCTCCGACGAGGAGCTGCTTAGTCACTACTTGGAGGAGAAGAATCAGTGCAAGGACTCCCAGATCACTACCATCATCCCTGAAATCGATGTGTGCAAGCACGAGCCTCGCGAGTTACCAGAACTGGCGTTCACAGGGGCGGAAAGTCTGGGCAGGAAATTTTTCTTTTTCTTCTTCTTTTGGTTTGCAAGGGAAGTGTCTTGGGACAATCAAGGAAAGTGGTACTTCTTCACTCCGCGTGATTACAAGTACAATAACAGCACTCGCTCCAACAGGACTACAGGGGAAGGGTTTTGGAAGATCACAGGCAAGGACCGTGCAATCAAGGCCCCCGGCTCCAAAGCTGTGATTGGGAGAAAGAGGACCTTGACCTTCTACAAACGTGGAGTGCCGAAATCCCAGAAGACCAGCTGGGTTATTCATGAGTATTATATCATTCAGGCACATTCTGATCCTCCTAAGCAGATAGGGGACTTTGTTGTCTGCTGCTTGAAGTACAAGTCAGACAACTCTGATTATGATAAGGATGCCCCATTCTGCGATCGAGGTAGCAGTAGCTGCAGTATGGCCTCCAATGTTGAAAATCAAGCTGAAGAAAACTGGGCATCGGAAGAGCTGGTAAATGTTGTTCCAATTCCCAGTGGAAATGATGATGAAACTGGTGGTGGTATGATTACAGAAGAGATTACAGAAGAAGAGGAATACCTGACTTTAGAGGAGGTGGAAGATATTCTTCACATTCCCATTGGCAATCCGCATGAATTTGAAGGCACGCAAACTGATCCTTGTCGTATACATAAGAATGATCCTTCAACCGCTGTTGAAGGCCAACCTGATAGAAGCAATTCATCTGATATGATTGAAGAGCTACTAGATGACCCAGAAAATCTGGATTCACTCTTTCATCCGCCTGCACCACCTCAACTGCATCAGTCACCGATGCATACCACCAATATCCATAATGATGAATGTCGTAAGAGGACATATCCATTTGAGGATAATGACCCTTCTCTTACGAAGAAGAAGAATAATATCTCAACCAATGATAACGACGAATTAGTTAGCAACATCACATCTAGTTCTGAAAATCAAGCCGCAAATATGATTCCAGAGGGATATTCTCAACTAGGAGCAAATCTGGGATCAGAATTTGATTCATTTCTGCCAAAGGATTACGATTATACATTACCATCAATAAACGGAGGACCGAGAGATTTTTCCCATGACAATAATTTCATTGAGTTGGATGATTTACTATCTTCATACGAAGGTATTGACTCTTCTCTCGCACAGTTCCTACATACAGTTATTGATGGAGGTAGAGGAGTAACCAGTGACAGAAACACCGAAGTAGTACATGGATCGGTAAACGATTATTCTGCATTAGCCATTGGAGCAACGCTCCAGCGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

486

Amino Acids

54.8

Weight (kDa)

4.72

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 159 1.5e-32 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
AccI GTMKAC 1 cut(s) 838
AccII CGCG 2 cut(s) 156, 283
AciI CCGC 8 cut(s) 9, 12, 185, 281, 800, 861, 953, 1140
AclWI GGATC 5 cut(s) 491, 821, 845, 1195, 1414
AcsI RAATTY 3 cut(s) 203, 806, 1193
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 4 cut(s) 269, 296, 542, 1401
AgsI TTSAA 6 cut(s) 538, 626, 812, 858, 869, 908
AluBI AGCT 9 cut(s) 6, 61, 389, 456, 603, 635, 661, 913, 1458
AluI AGCT 9 cut(s) 6, 61, 389, 456, 603, 635, 661, 913, 1458
AlwI GGATC 5 cut(s) 491, 821, 845, 1195, 1414
AlwNI CAGNNNCTG 1 cut(s) 603
AoxI GGCC 3 cut(s) 372, 612, 871
ApeKI GCWGC 4 cut(s) 6, 61, 531, 603
ApoI RAATTY 3 cut(s) 203, 806, 1193
Asp700I GAANNNNTTC 3 cut(s) 206, 774, 889
AspS9I GGNCC 4 cut(s) 358, 373, 408, 1250
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 1 cut(s) 972
AvaII GGWCC 3 cut(s) 358, 408, 1250
BarI GAAGNNNNNNTAC 2 cut(s) 1309, 1341
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 3 cut(s) 48, 518, 590
BccI CCATC 4 cut(s) 51, 125, 1243, 1352
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 1406, 1440
BciVI GTATCC 1 cut(s) 26
BcnI CCSGG 1 cut(s) 378
BfaI CTAG 3 cut(s) 917, 1122, 1172
BfmI CTRYAG 2 cut(s) 324, 604
BfuI GTATCC 1 cut(s) 26
BisI GCNGC 7 cut(s) 7, 10, 13, 62, 532, 604, 1140
BlsI GCNGC 7 cut(s) 8, 11, 14, 63, 533, 605, 1141
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 3 cut(s) 358, 408, 1250
BmgT120I GGNCC 4 cut(s) 358, 373, 408, 1250
BmiI GGNNCC 2 cut(s) 375, 382
BmrFI CCNGG 1 cut(s) 378
BmrI ACTGGG 2 cut(s) 655, 678
BmsI GCATC 4 cut(s) 562, 659, 975, 982
BmuI ACTGGG 2 cut(s) 655, 678
BpiI GAAGAC 1 cut(s) 455
BplI GAGNNNNNCTC 1 cut(s) 1431
BpmI CTGGAG 1 cut(s) 1433
Bpu10I CCTNAGC 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 376
Bse1I ACTGG 5 cut(s) 174, 650, 684, 709, 1377
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 5 cut(s) 43, 120, 577, 949, 1297
BseNI ACTGG 5 cut(s) 174, 650, 684, 709, 1377
BseRI GAGGAG 4 cut(s) 71, 95, 776, 1383
BseXI GCAGC 3 cut(s) 48, 518, 590
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 942
Bsh1236I CGCG 2 cut(s) 156, 283
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 3 cut(s) 374, 614, 873
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 589
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 2 cut(s) 263, 530
BsmFI GGGAC 2 cut(s) 263, 530
BsnI GGCC 3 cut(s) 374, 614, 873
Bsp143I GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 8 cut(s) 9, 12, 185, 281, 800, 861, 953, 1140
BspANI GGCC 3 cut(s) 374, 614, 873
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 156, 283
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 375, 382
BspMAI CTGCAG 1 cut(s) 608
BspPI GGATC 5 cut(s) 491, 821, 845, 1195, 1414
BspQI GCTCTTC 2 cut(s) 651, 903
BsrI ACTGG 5 cut(s) 174, 650, 684, 709, 1377
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
BssNAI GTATAC 1 cut(s) 839
BssSI CACGAG 1 cut(s) 146
Bst1107I GTATAC 1 cut(s) 839
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 4 cut(s) 22, 362, 1338, 1351
Bst6I CTCTTC 5 cut(s) 651, 720, 735, 903, 1330
BstAPI GCANNNNNTGC 1 cut(s) 603
BstC8I GCNNGC 3 cut(s) 143, 818, 957
BstDEI CTNAG 2 cut(s) 65, 504
BstF5I GGATG 5 cut(s) 43, 120, 577, 949, 1297
BstFNI CGCG 2 cut(s) 156, 283
BstKTI GATC 8 cut(s) 111, 348, 499, 589, 829, 853, 1190, 1409
BstMBI GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 4 cut(s) 12, 371, 603, 1439
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 2 cut(s) 324, 604
BstUI CGCG 2 cut(s) 156, 283
BstV1I GCAGC 3 cut(s) 48, 518, 590
BstV2I GAAGAC 1 cut(s) 455
BstZ17I GTATAC 1 cut(s) 839
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 3 cut(s) 374, 614, 873
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 5 cut(s) 43, 120, 577, 949, 1297
BtsIMutI CAGTG 3 cut(s) 98, 691, 1384
BtuMI TCGCGA 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 143, 818, 957
CaiI CAGNNNCTG 1 cut(s) 603
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 4 cut(s) 358, 373, 408, 1250
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 4 cut(s) 268, 295, 541, 1400
CspCI CAANNNNNGTGG 2 cut(s) 245, 280
CviAII CATG 4 cut(s) 467, 803, 1268, 1403
CviQI GTAC 4 cut(s) 268, 295, 541, 1400
DdeI CTNAG 2 cut(s) 65, 504
DpnI GATC 8 cut(s) 110, 347, 498, 588, 828, 852, 1189, 1408
DpnII GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
Eam1104I CTCTTC 5 cut(s) 651, 720, 735, 903, 1330
EarI CTCTTC 5 cut(s) 651, 720, 735, 903, 1330
EciI GGCGGA 2 cut(s) 200, 942
Eco47I GGWCC 3 cut(s) 358, 408, 1250
Eco57I CTGAAG 1 cut(s) 657
EcoO109I RGGNCCY 2 cut(s) 373, 408
EcoT22I ATGCAT 1 cut(s) 990
FaeI CATG 4 cut(s) 470, 806, 1271, 1406
FalI AAGNNNNNCTT 6 cut(s) 254, 286, 395, 427, 762, 794
FaqI GGGAC 2 cut(s) 263, 530
FatI CATG 4 cut(s) 466, 802, 1267, 1402
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 7 cut(s) 7, 10, 13, 62, 532, 604, 1140
FokI GGATG 5 cut(s) 30, 107, 584, 936, 1304
Fsp4HI GCNGC 7 cut(s) 7, 10, 13, 62, 532, 604, 1140
FspBI CTAG 3 cut(s) 917, 1122, 1172
GluI GCNGC 7 cut(s) 7, 10, 13, 62, 532, 604, 1140
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 1 cut(s) 1433
HaeIII GGCC 3 cut(s) 374, 614, 873
HapII CCGG 1 cut(s) 378
Hin1II CATG 4 cut(s) 470, 806, 1271, 1406
HinfI GANTC 7 cut(s) 39, 88, 101, 938, 1150, 1199, 1322
HpaII CCGG 1 cut(s) 378
HphI GGTGA 1 cut(s) 972
Hpy166II GTNNAC 3 cut(s) 177, 839, 1414
Hpy188I TCNGA 9 cut(s) 27, 52, 496, 550, 559, 655, 898, 1129, 1192
Hpy188III TCNNGA 4 cut(s) 155, 467, 935, 1154
Hpy8I GTNNAC 3 cut(s) 177, 839, 1414
Hpy99I CGWCG 2 cut(s) 56, 1100
HpyAV CCTTC 8 cut(s) 22, 326, 427, 806, 863, 864, 1062, 1307
HpyCH4III ACNGT 4 cut(s) 22, 362, 1338, 1351
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 9 cut(s) 96, 141, 233, 365, 606, 959, 973, 988, 1427
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 371, 603, 1439
HpyF3I CTNAG 2 cut(s) 65, 504
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 4 cut(s) 470, 806, 1271, 1406
Kzo9I GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
LguI GCTCTTC 2 cut(s) 651, 903
LmnI GCTCC 6 cut(s) 58, 317, 386, 1175, 1439, 1452
Lsp1109I GCAGC 3 cut(s) 48, 518, 590
LweI GCATC 4 cut(s) 562, 659, 975, 982
MaeI CTAG 3 cut(s) 917, 1122, 1172
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 5 cut(s) 68, 159, 978, 1372, 1379
MalI GATC 8 cut(s) 110, 347, 498, 588, 828, 852, 1189, 1408
MboI GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
MluCI AATT 7 cut(s) 203, 678, 806, 889, 1100, 1193, 1276
MlyI GAGTC 2 cut(s) 95, 1316
MmeI TCCRAC 3 cut(s) 75, 339, 1269
Mph1103I ATGCAT 1 cut(s) 990
MroXI GAANNNNTTC 3 cut(s) 206, 774, 889
MslI CAYNNNNRTG 2 cut(s) 846, 1008
MspA1I CMGCKG 2 cut(s) 456, 863
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 156, 283
MwoI GCNNNNNNNGC 4 cut(s) 12, 371, 603, 1439
NciI CCSGG 1 cut(s) 378
NdeII GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
NlaIII CATG 4 cut(s) 470, 806, 1271, 1406
NlaIV GGNNCC 2 cut(s) 375, 382
NmuCI GTSAC 3 cut(s) 68, 978, 1379
NruI TCGCGA 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 990
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 2 cut(s) 651, 903
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 3 cut(s) 206, 774, 889
PfeI GAWTC 5 cut(s) 39, 88, 938, 1150, 1199
PkrI GCNGC 7 cut(s) 8, 11, 14, 63, 533, 605, 1141
Ple19I CGATCG 1 cut(s) 589
PleI GAGTC 2 cut(s) 95, 1316
PpsI GAGTC 2 cut(s) 95, 1316
PpuMI RGGWCCY 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 375, 382
PspPI GGNCC 4 cut(s) 358, 373, 408, 1250
PspPPI RGGWCCY 1 cut(s) 408
PsrI GAACNNNNNNTAC 2 cut(s) 657, 689
PstI CTGCAG 1 cut(s) 608
PstNI CAGNNNCTG 1 cut(s) 603
PvuI CGATCG 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 456
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 4 cut(s) 269, 296, 542, 1401
RsaNI GTAC 4 cut(s) 268, 295, 541, 1400
RseI CAYNNNNRTG 2 cut(s) 846, 1008
SapI GCTCTTC 2 cut(s) 651, 903
SatI GCNGC 7 cut(s) 7, 10, 13, 62, 532, 604, 1140
Sau3AI GATC 8 cut(s) 108, 345, 496, 586, 826, 850, 1187, 1406
Sau96I GGNCC 4 cut(s) 358, 373, 408, 1250
SchI GAGTC 2 cut(s) 95, 1316
ScrFI CCNGG 1 cut(s) 378
SfaNI GCATC 4 cut(s) 562, 659, 975, 982
SfcI CTRYAG 2 cut(s) 324, 604
SinI GGWCC 3 cut(s) 358, 408, 1250
SmiMI CAYNNNNRTG 2 cut(s) 846, 1008
Sse9I AATT 7 cut(s) 203, 678, 806, 889, 1100, 1193, 1276
SsiI CCGC 8 cut(s) 9, 12, 185, 281, 800, 861, 953, 1140
SspMI CTAG 3 cut(s) 917, 1122, 1172
StyD4I CCNGG 1 cut(s) 376
TaaI ACNGT 4 cut(s) 22, 362, 1338, 1351
TaiI ACGT 1 cut(s) 430
TaqI TCGA 2 cut(s) 132, 589
TaqII GACCGA 1 cut(s) 1267
TasI AATT 7 cut(s) 203, 678, 806, 889, 1100, 1193, 1276
TatI WGTACW 3 cut(s) 294, 540, 1399
TauI GCSGC 3 cut(s) 12, 15, 1142
TfiI GAWTC 5 cut(s) 39, 88, 938, 1150, 1199
TscAI CASTG 3 cut(s) 98, 691, 1384
TseFI GTSAC 3 cut(s) 68, 978, 1379
TseI GCWGC 4 cut(s) 6, 61, 531, 603
Tsp45I GTSAC 3 cut(s) 68, 978, 1379
TspDTI ATGAA 9 cut(s) 455, 714, 819, 882, 938, 1026, 1191, 1270, 1296
TspGWI ACGGA 1 cut(s) 1260
TspRI CASTG 3 cut(s) 98, 691, 1384
VpaK11BI GGWCC 3 cut(s) 358, 408, 1250
XapI RAATTY 3 cut(s) 203, 806, 1193
XmiI GTMKAC 1 cut(s) 838
XmnI GAANNNNTTC 3 cut(s) 206, 774, 889
XspI CTAG 3 cut(s) 917, 1122, 1172
Zsp2I ATGCAT 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.