Rh1BG350700

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002400
Physical Location & Seq
Reverse (-)
48385054 .. 48391961
6908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG350700.1

Sequence Viewer

Length: 987 bp
ATGGTTACATTGAAAACACCTGAAAAAGAAAATGGGTTTCGTCCCAATTTTAAGAGGCCTGGAGTTTATGCAGTTCGAGATTTTCCTGAAGGTTGCGGACGATTTATGGCTCGAGTGCAGCCAAGTGAATGTGAAGAGGGTGATGAAGATATGAACACATTACCAAGGAAAAATCGCTCCCTGCCTAGTCCTCGAGTTGATGTTGATGTTGTTCGAGATTTTCCTAAAGGTTGTGGACAGATGGTAGTTTCTTCTTTGAAAGATGCTGAAGAGAAGAGTTTGGTTCCAGACGTTAAAGAATGCGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGTGAAGAAAGCTTTGGCTGCATATCAAGAGACTTTGATTGAGATGGGTGCAGAATGCGATGCTAATTTCAACAGACTAAAGGGTTGGAAATTGTATGTTGAAGCAGCAATGTATCTTAAGAAGAAAGATAAATGGGTTAACACCAAGAAACAAACAGGGGACATTCCAGGAGTGAAGGTTGGTGACAAGTTTCGGTATAGACCTGAATTAGCTATTGTTGGTCTTCATAGTCACTTTCAATGCGGTATTGATTATATGACAGAGGACGGGGAAATGATTGCCACAAGTATTGTTGAATCTGGCCGCTATGCGAACGAGACAGAATCACCTGATGTCATGATATACACAGGTCAAGGTGGAAATCCACAGGTTTCGAAAGGGGAACCAATAGATCAAAAGCCCGAGCGTGGAAACCGTGCACTGATGAATAGCAAGGCAACAGGAACTCCTGTGAGGGTAATCCGGAGCTATAAATTTCGGGAGGAATCTGGCTATGTTTATCATGGTCTCTACATTGTGGATAATTTTTGGCGAAAAAGAGGAGATTATGGTAAGTTTGTGTTTAAATTTTTGTTGAGGAGGATTTCGGGTCAACCAAAACTCACACTTTGCAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

328

Amino Acids

37.09

Weight (kDa)

9.11

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 174 - 320 6.1e-47 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 828
AciI CCGC 3 cut(s) 96, 609, 670
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 2 cut(s) 839, 932
AcuI CTGAAG 2 cut(s) 108, 288
AfiI CCNNNNNNNGG 1 cut(s) 773
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 6 cut(s) 13, 259, 436, 467, 605, 662
AjnI CCWGG 2 cut(s) 58, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 356, 377, 578, 834
AluI AGCT 4 cut(s) 356, 377, 578, 834
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 3 cut(s) 389, 677, 878
Alw44I GTGCAC 1 cut(s) 783
Ama87I CYCGRG 3 cut(s) 111, 192, 767
Aor13HI TCCGGA 1 cut(s) 828
AoxI GGCC 2 cut(s) 56, 667
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 4 cut(s) 118, 307, 383, 470
ApoI RAATTY 2 cut(s) 839, 932
AsuHPI GGTGA 4 cut(s) 152, 379, 560, 684
AsuII TTCGAA 1 cut(s) 740
AvaI CYCGRG 3 cut(s) 111, 192, 767
BaeGI GKGCMC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 4 cut(s) 130, 319, 370, 482
BccI CCATC 2 cut(s) 235, 403
BciT130I CCWGG 2 cut(s) 60, 534
BcoDI GTCTC 3 cut(s) 389, 677, 878
BfaI CTAG 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 482
BisI GCNGC 5 cut(s) 119, 308, 384, 471, 670
BlsI GCNGC 5 cut(s) 120, 309, 385, 472, 671
Bme1390I CCNGG 2 cut(s) 60, 534
BmeT110I CYCGRG 3 cut(s) 111, 192, 767
BmiI GGNNCC 2 cut(s) 285, 750
BmrFI CCNGG 2 cut(s) 60, 534
BmsI GCATC 2 cut(s) 253, 415
BpiI GAAGAC 1 cut(s) 581
BpmI CTGGAG 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 878
BsaJI CCNNGG 1 cut(s) 164
BsaWI WCCGGW 1 cut(s) 828
BsaXI ACNNNNNCTCC 4 cut(s) 528, 558, 796, 826
Bsc4I CCNNNNNNNGG 1 cut(s) 773
Bse3DI GCAATG 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 828
BseBI CCWGG 2 cut(s) 60, 534
BseDI CCNNGG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 773
BseMI GCAATG 1 cut(s) 480
BseRI GAGGAG 2 cut(s) 921, 958
BseSI GKGCMC 1 cut(s) 787
BseXI GCAGC 4 cut(s) 130, 319, 370, 482
BsgI GTGCAG 2 cut(s) 137, 435
BshFI GGCC 2 cut(s) 58, 669
BsiHKAI GWGCWC 1 cut(s) 787
BsiHKCI CYCGRG 3 cut(s) 111, 192, 767
BsiSI CCGG 1 cut(s) 829
BslFI GGGAC 2 cut(s) 27, 539
BslI CCNNNNNNNGG 1 cut(s) 773
BsmAI GTCTC 3 cut(s) 389, 677, 878
BsmFI GGGAC 2 cut(s) 27, 539
BsmI GAATGC 2 cut(s) 305, 425
BsnI GGCC 2 cut(s) 58, 669
Bso31I GGTCTC 1 cut(s) 878
BsoBI CYCGRG 3 cut(s) 111, 192, 767
Bsp119I TTCGAA 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 787
Bsp13I TCCGGA 1 cut(s) 828
Bsp143I GATC 1 cut(s) 757
BspACI CCGC 3 cut(s) 96, 609, 670
BspANI GGCC 2 cut(s) 58, 669
BspEI TCCGGA 1 cut(s) 828
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 285, 750
BspT104I TTCGAA 1 cut(s) 740
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 480
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 1 cut(s) 757
BssT1I CCWWGG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 60, 534
Bst4CI ACNGT 2 cut(s) 782, 983
Bst6I CTCTTC 3 cut(s) 129, 264, 269
BstAFI CTTAAG 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 305
BstKTI GATC 1 cut(s) 760
BstMAI GTCTC 3 cut(s) 389, 677, 878
BstMBI GATC 1 cut(s) 757
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 2 cut(s) 60, 534
BstSCI CCNGG 2 cut(s) 58, 532
BstSLI GKGCMC 1 cut(s) 787
BstV1I GCAGC 4 cut(s) 130, 319, 370, 482
BstV2I GAAGAC 1 cut(s) 581
BsuRI GGCC 2 cut(s) 58, 669
BtgZI GCGATG 1 cut(s) 438
BtsIMutI CAGTG 1 cut(s) 785
Cac8I GCNNGC 1 cut(s) 305
CciI TCATGA 1 cut(s) 702
CspCI CAANNNNNGTGG 2 cut(s) 637, 672
CviAII CATG 2 cut(s) 703, 869
DpnI GATC 1 cut(s) 759
DpnII GATC 1 cut(s) 757
DraI TTTAAA 1 cut(s) 931
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 3 cut(s) 129, 264, 269
EarI CTCTTC 3 cut(s) 129, 264, 269
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 878
Eco57I CTGAAG 2 cut(s) 108, 288
Eco88I CYCGRG 3 cut(s) 111, 192, 767
EcoRII CCWGG 2 cut(s) 58, 532
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 2 cut(s) 706, 872
FaqI GGGAC 2 cut(s) 27, 539
FatI CATG 2 cut(s) 702, 868
Fnu4HI GCNGC 5 cut(s) 119, 308, 384, 471, 670
Fsp4HI GCNGC 5 cut(s) 119, 308, 384, 471, 670
FspBI CTAG 1 cut(s) 186
GluI GCNGC 5 cut(s) 119, 308, 384, 471, 670
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 58, 669
HapII CCGG 1 cut(s) 829
Hin1II CATG 2 cut(s) 706, 872
HincII GTYRAC 2 cut(s) 505, 959
HindII GTYRAC 2 cut(s) 505, 959
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 3 cut(s) 662, 689, 851
HpaI GTTAAC 1 cut(s) 505
HpaII CCGG 1 cut(s) 829
HphI GGTGA 4 cut(s) 152, 379, 560, 684
Hpy166II GTNNAC 4 cut(s) 236, 505, 785, 959
Hpy188III TCNNGA 8 cut(s) 77, 86, 215, 287, 392, 703, 829, 845
Hpy8I GTNNAC 4 cut(s) 236, 505, 785, 959
HpyAV CCTTC 2 cut(s) 83, 535
HpyCH4III ACNGT 2 cut(s) 782, 983
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 7 cut(s) 71, 118, 338, 386, 416, 785, 978
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 2 cut(s) 706, 872
Kpn2I TCCGGA 1 cut(s) 828
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 1 cut(s) 757
LmnI GCTCC 2 cut(s) 182, 831
Lsp1109I GCAGC 4 cut(s) 130, 319, 370, 482
LweI GCATC 2 cut(s) 253, 415
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 4 cut(s) 4, 320, 548, 596
MalI GATC 1 cut(s) 759
MboI GATC 1 cut(s) 757
MboII GAAGA 8 cut(s) 146, 158, 243, 281, 286, 382, 499, 581
MhlI GDGCHC 1 cut(s) 787
MluCI AATT 7 cut(s) 46, 430, 455, 572, 839, 889, 932
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 9 cut(s) 48, 130, 201, 622, 813, 841, 899, 936, 939
MroI TCCGGA 1 cut(s) 828
MseI TTAA 6 cut(s) 51, 294, 483, 504, 930, 985
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 1 cut(s) 829
MspR9I CCNGG 2 cut(s) 60, 534
Mva1269I GAATGC 2 cut(s) 305, 425
MvaI CCWGG 2 cut(s) 60, 534
MwoI GCNNNNNNNGC 1 cut(s) 383
NdeII GATC 1 cut(s) 757
NlaIII CATG 2 cut(s) 706, 872
NlaIV GGNNCC 2 cut(s) 285, 750
NmuCI GTSAC 3 cut(s) 320, 548, 596
NspV TTCGAA 1 cut(s) 740
PaeR7I CTCGAG 2 cut(s) 111, 192
PagI TCATGA 1 cut(s) 702
PceI AGGCCT 1 cut(s) 58
PctI GAATGC 2 cut(s) 305, 425
PfeI GAWTC 3 cut(s) 662, 689, 851
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 5 cut(s) 120, 309, 385, 472, 671
Psp6I CCWGG 2 cut(s) 58, 532
PspGI CCWGG 2 cut(s) 58, 532
PspN4I GGNNCC 2 cut(s) 285, 750
PspXI VCTCGAGB 2 cut(s) 111, 192
SaqAI TTAA 6 cut(s) 51, 294, 483, 504, 930, 985
SatI GCNGC 5 cut(s) 119, 308, 384, 471, 670
Sau3AI GATC 1 cut(s) 757
ScrFI CCNGG 2 cut(s) 60, 534
SduI GDGCHC 1 cut(s) 787
SfaNI GCATC 2 cut(s) 253, 415
Sfr274I CTCGAG 2 cut(s) 111, 192
SfuI TTCGAA 1 cut(s) 740
SlaI CTCGAG 2 cut(s) 111, 192
SmlI CTYRAG 3 cut(s) 111, 192, 482
SmoI CTYRAG 3 cut(s) 111, 192, 482
Sse9I AATT 7 cut(s) 46, 430, 455, 572, 839, 889, 932
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 3 cut(s) 96, 609, 670
SspMI CTAG 1 cut(s) 186
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 2 cut(s) 58, 532
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 782, 983
TaiI ACGT 1 cut(s) 294
TaqI TCGA 5 cut(s) 76, 112, 193, 214, 740
TasI AATT 7 cut(s) 46, 430, 455, 572, 839, 889, 932
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 3 cut(s) 662, 689, 851
Tru1I TTAA 6 cut(s) 51, 294, 483, 504, 930, 985
Tru9I TTAA 6 cut(s) 51, 294, 483, 504, 930, 985
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 3 cut(s) 320, 548, 596
TseI GCWGC 4 cut(s) 118, 307, 383, 470
Tsp45I GTSAC 3 cut(s) 320, 548, 596
TspDTI ATGAA 4 cut(s) 159, 167, 581, 806
TspRI CASTG 1 cut(s) 792
Vha464I CTTAAG 1 cut(s) 482
VneI GTGCAC 1 cut(s) 783
XapI RAATTY 2 cut(s) 839, 932
XhoI CTCGAG 2 cut(s) 111, 192
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.