Rw1G005820

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002400
Physical Location & Seq
Reverse (-)
11773110 .. 11774227
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005820.1

Sequence Viewer

Length: 936 bp
ATGGCCATAGTGAAAGTACATGGAACAATACACCATGGTTGCTTTATTCCCGTGAAGAGGGCAAGAGTTGATGTAATTCGACACCTTCCTAGAGGGCGCGGATCAAATGTTAGGCGGCAAAAGGGTGAATGCCAAGATCTTGATTATATCGTTGAACCTGCTAATAAAGATGATGAACCTGCTGCGATGATTGATAAGGTGTGTGCGCCTCGTGAAAGGATTGATAAGAGACTGGAGAGCAAAAGTTTACCACCACCGGATTGCCAATGCCATAATGAGAAGGTTTCTGTTGCTAGTTACCGGTTGACTGATCCTGATGTGGAGTTCAAAGATCATTGCAAGTTGAAGAAGACCCTTGATGCATATAAGGAGACACTGACCAAGTTCAACAAACGAAATTGCTCAACTTACATTTTAGCAGCAATTCGTTTAAGGAATGAAGGAAAATGGCTCAACATGAGGAAACAATTAGGAGCCATTCGAGGAGTAAAAGTTGGTGACAAGTTTCGATATAGAGCTCAACTAAATATCGTTGGGCTTCATCGACATTTCACCAATGGAATTGATTATATAAAGAGCAGAGATGGGAAAATACTAGTCACAAGTATTGTTGATTCTGGTCGTTATGCCAACAATATGCAATGTTCCGATGTATTGATCTATTCTGGTGAAGGCGGAAATTCCCCGTTTAATAAATTGAAACCAACAGATCAGACACTTGAGCGTGGAAACCTTGCATTGAAGAATAGCACAGAAGCACAAACTCCTGTAAGGGTAATCCGCACCTTTAAACTTTCAAAGAGTTCATGTTATGTTTACTATGGTCTATACATTGTAGAGCATCTTTCAAAAGAAAGGGGGCAATTTGGTAAGATGGTTTATAAATTTAGGTTGAGAAGGAAGGCAGGACAACCAGAAATAACATTCAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

311

Amino Acids

35.62

Weight (kDa)

9.8

Isoelectric Point (pI)

28.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 157 - 304 4.1e-45 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
Acc36I ACCTGC 2 cut(s) 166, 187
AccII CGCG 1 cut(s) 99
AciI CCGC 4 cut(s) 99, 115, 675, 781
AclWI GGATC 2 cut(s) 109, 305
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 679, 884
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 57
AgeI ACCGGT 1 cut(s) 300
AgsI TTSAA 9 cut(s) 155, 328, 346, 388, 700, 742, 798, 849, 928
AhlI ACTAGT 1 cut(s) 595
AluBI AGCT 1 cut(s) 518
AluI AGCT 1 cut(s) 518
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 2 cut(s) 223, 365
AlwI GGATC 2 cut(s) 109, 305
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 182, 419
ApoI RAATTY 2 cut(s) 679, 884
AsiGI ACCGGT 1 cut(s) 300
AspLEI GCGC 2 cut(s) 99, 208
AsuHPI GGTGA 4 cut(s) 137, 509, 544, 680
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 520
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 2 cut(s) 169, 431
BccI CCATC 2 cut(s) 578, 868
BcoDI GTCTC 2 cut(s) 223, 365
BcuI ACTAGT 1 cut(s) 595
BfaI CTAG 3 cut(s) 90, 294, 596
BfuAI ACCTGC 2 cut(s) 166, 187
BglII AGATCT 1 cut(s) 136
BisI GCNGC 3 cut(s) 116, 183, 420
BlsI GCNGC 3 cut(s) 117, 184, 421
BmiI GGNNCC 1 cut(s) 475
BmsI GCATC 2 cut(s) 349, 850
BpiI GAAGAC 1 cut(s) 356
BpmI CTGGAG 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 34
BsaWI WCCGGW 2 cut(s) 256, 300
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 2 cut(s) 334, 647
BseDI CCNNGG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 2 cut(s) 334, 647
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 498
BseXI GCAGC 2 cut(s) 169, 431
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 520
BsiSI CCGG 2 cut(s) 257, 301
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 2 cut(s) 223, 365
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 6 cut(s) 101, 136, 310, 331, 657, 709
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 4 cut(s) 99, 115, 675, 781
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 475
BspMI ACCTGC 2 cut(s) 166, 187
BspPI GGATC 2 cut(s) 109, 305
BsrDI GCAATG 2 cut(s) 334, 647
BsrFI RCCGGY 1 cut(s) 300
BsrI ACTGG 1 cut(s) 237
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 6 cut(s) 101, 136, 310, 331, 657, 709
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 34
Bst2BI CACGAG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 933
Bst6I CTCTTC 1 cut(s) 50
BstDSI CCRYGG 1 cut(s) 34
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 2 cut(s) 99, 208
BstKTI GATC 6 cut(s) 104, 139, 313, 334, 660, 712
BstMAI GTCTC 2 cut(s) 223, 365
BstMBI GATC 6 cut(s) 101, 136, 310, 331, 657, 709
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 169, 431
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 34
BtgZI GCGATG 1 cut(s) 200
BtsIMutI CAGTG 1 cut(s) 374
BveI ACCTGC 2 cut(s) 166, 187
CfoI GCGC 2 cut(s) 99, 208
Cfr10I RCCGGY 1 cut(s) 300
Csp6I GTAC 1 cut(s) 17
CspAI ACCGGT 1 cut(s) 300
CviAII CATG 4 cut(s) 20, 35, 457, 807
CviJI RGCY 5 cut(s) 5, 451, 476, 518, 538
CviKI_1 RGCY 5 cut(s) 5, 451, 476, 518, 538
CviQI GTAC 1 cut(s) 17
DpnI GATC 6 cut(s) 103, 138, 312, 333, 659, 711
DpnII GATC 6 cut(s) 101, 136, 310, 331, 657, 709
DraI TTTAAA 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 518
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 520
Eco53kI GAGCTC 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 518
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 4 cut(s) 23, 38, 460, 810
FatI CATG 4 cut(s) 19, 34, 456, 806
Fnu4HI GCNGC 3 cut(s) 116, 183, 420
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 3 cut(s) 116, 183, 420
FspBI CTAG 3 cut(s) 90, 294, 596
GlaI GCGC 2 cut(s) 98, 207
GluI GCNGC 3 cut(s) 116, 183, 420
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 257, 301
HhaI GCGC 2 cut(s) 99, 208
Hin1II CATG 4 cut(s) 23, 38, 460, 810
Hin6I GCGC 2 cut(s) 97, 206
HinP1I GCGC 2 cut(s) 97, 206
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 1 cut(s) 614
HpaII CCGG 2 cut(s) 257, 301
HphI GGTGA 4 cut(s) 137, 509, 544, 680
Hpy166II GTNNAC 3 cut(s) 248, 306, 817
Hpy188I TCNGA 2 cut(s) 649, 714
Hpy188III TCNNGA 3 cut(s) 140, 212, 314
Hpy8I GTNNAC 3 cut(s) 248, 306, 817
HpyAV CCTTC 6 cut(s) 95, 274, 434, 665, 891, 895
HpyCH4III ACNGT 1 cut(s) 933
HpyCH4V TGCA 4 cut(s) 339, 362, 640, 737
Hsp92II CATG 4 cut(s) 23, 38, 460, 810
HspAI GCGC 2 cut(s) 97, 206
Kzo9I GATC 6 cut(s) 101, 136, 310, 331, 657, 709
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 2 cut(s) 169, 431
LweI GCATC 2 cut(s) 349, 850
MaeI CTAG 3 cut(s) 90, 294, 596
MaeIII GTNAC 3 cut(s) 296, 497, 598
MalI GATC 6 cut(s) 103, 138, 312, 333, 659, 711
MboI GATC 6 cut(s) 101, 136, 310, 331, 657, 709
MboII GAAGA 4 cut(s) 67, 358, 361, 754
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 520
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 75, 397, 423, 467, 561, 679, 695, 863, 884
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 51, 86, 219, 453, 476
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 364
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 431, 690, 789
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 257, 301
Mva1269I GAATGC 1 cut(s) 134
MvnI CGCG 1 cut(s) 99
NcoI CCATGG 1 cut(s) 34
NdeII GATC 6 cut(s) 101, 136, 310, 331, 657, 709
NlaIII CATG 4 cut(s) 23, 38, 460, 810
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 497, 598
NsiI ATGCAT 1 cut(s) 364
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 614
PinAI ACCGGT 1 cut(s) 300
PkrI GCNGC 3 cut(s) 117, 184, 421
PsiI TTATAA 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 520
PspN4I GGNNCC 1 cut(s) 475
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 3 cut(s) 431, 690, 789
SatI GCNGC 3 cut(s) 116, 183, 420
Sau3AI GATC 6 cut(s) 101, 136, 310, 331, 657, 709
SduI GDGCHC 1 cut(s) 520
SetI ASST 9 cut(s) 87, 160, 181, 201, 285, 520, 735, 788, 893
SfaNI GCATC 2 cut(s) 349, 850
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
SpeI ACTAGT 1 cut(s) 595
Sse9I AATT 9 cut(s) 75, 397, 423, 467, 561, 679, 695, 863, 884
SsiI CCGC 4 cut(s) 99, 115, 675, 781
SspMI CTAG 3 cut(s) 90, 294, 596
SstI GAGCTC 1 cut(s) 520
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 1 cut(s) 933
TaqI TCGA 4 cut(s) 79, 481, 508, 544
TasI AATT 9 cut(s) 75, 397, 423, 467, 561, 679, 695, 863, 884
TatI WGTACW 1 cut(s) 16
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 3 cut(s) 431, 690, 789
Tru9I TTAA 3 cut(s) 431, 690, 789
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 2 cut(s) 497, 598
TseI GCWGC 2 cut(s) 182, 419
Tsp45I GTSAC 2 cut(s) 497, 598
TspDTI ATGAA 4 cut(s) 189, 453, 530, 795
TspRI CASTG 1 cut(s) 381
XapI RAATTY 2 cut(s) 679, 884
XspI CTAG 3 cut(s) 90, 294, 596
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.