Rw6G029500

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002400
Physical Location & Seq
Reverse (-)
53606384 .. 53607688
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G029500.1

Sequence Viewer

Length: 1305 bp
ATGGCTATCGTGAAAGTACGTGGAACCCTCAGAGACGGTTTATTTACTCCAAAATCGAAGAGGCCAAGAGTGGATGATTTTGGAAATTTTCCCAATGGGCGTGGATCATATAGAGTTATGGTGCAAAGTAGTGAAAGTGAAGATCACAATTCTCTAATTGATCGTGATTTGGTTAAATCATCAAAAAATCTCCAGCATAAGAGAAATGATGGGATTTCGCATTTTTCTATTACGGCTACACCACAAGAAATTTTGCCTTCACCACAGAGTGTATTGAAAAGTAACTCTGTAAAGAGCAAGGAGGATCGGAACACTTACCTATCAATCCACCAAGGATTGAGGAAGCGACAACCTTTGTCTGATGAATACCGACCTGAAAGGACGGCTGTATGTGGAAATTTTGATTTTAGTCACCAGCATGTGCATAATTTGGAAGATAAAGATAATACCGAGGTGAAGAAGGATCTTGATGTGATCGAAGATACTTTGGTCAAATCCAAGAAACAAAATTGGAGCAACGAATATCAGCACAAAATCAACACAGTGTCCAAAAATGTTGTTACAACTTTCAATTCAAAAAATAGACATTTCGTCTTGGCGAGAAAGAGACAACCACTGTGGGACCACCAACAACCTTACCAGATGAGTATGCATGAGGAGTTTACTAGTCAAAAATCGGTGGAAGTGGAACAGAAAAATCGTAACAAGGTCAAGGGGGCTTTGGAAGCCTATAGAGAGATAATGACTAAGTCCGAGGCACAAAACTGTAATTGGAGTTACATTAAAGCAGCAATGGATCTCAAAAAACAAGGAAAATGGGTTAACAAGATGAAGCAAGTAGGATCGATTGCAGGAGTACAAGTTGGTGATAGGTTTCGACATAGAGCTCAACTAACCATTGTTGGTCTTCATCGACAATTTACATGTGGTATTGATTATATGTGGAGGAATGACGGAAAAATTTTGGCCACAAGTATTGTTGATTCTGGTCGCTATGTTAACAATTTGCAATCTTCTGATATACTGATCTACTCTGGTCAAGGTGGAAAGTCTCTGTTTCATAAAAGTAAACCGACAGATCAAATAATGCAAGGTGGAAACCTTGCATTAAGGAATAGCAAAGAAGAAGGAACTCCTATAAGGGTAATTCGAAGGTTGAAACATTCTAAGGGTTCGTGTTATGTTTACTATGGATTATACACCGTAGAAAGAGTTCTGCAAGAAAGACAACTTGGTATTATGATCTATAAATTTACGTTGAGAAGGAAGTCGGGACAACCAGAGCTTTCATTTGACAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

434

Amino Acids

50.0

Weight (kDa)

9.8

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 280 - 426 1e-40 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 112, 312, 471, 804, 850
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 5 cut(s) 85, 249, 397, 960, 1250
AdeI CACNNNGTG 1 cut(s) 269
AfaI GTAC 2 cut(s) 18, 858
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 4 cut(s) 277, 571, 576, 1159
AhdI GACNNNNNGTC 1 cut(s) 590
AhlI ACTAGT 1 cut(s) 665
AjuI GAANNNNNNNTTGG 2 cut(s) 1215, 1247
AluBI AGCT 2 cut(s) 887, 1285
AluI AGCT 2 cut(s) 887, 1285
Alw21I GWGCWC 1 cut(s) 889
Alw26I GTCTC 3 cut(s) 27, 601, 1056
AlwI GGATC 5 cut(s) 112, 312, 471, 804, 850
AoxI GGCC 2 cut(s) 62, 966
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 5 cut(s) 85, 249, 397, 960, 1250
Asp700I GAANNNNTTC 1 cut(s) 1212
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 4 cut(s) 252, 404, 466, 878
AsuII TTCGAA 1 cut(s) 1150
AvaII GGWCC 1 cut(s) 622
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 889
BbvI GCAGC 1 cut(s) 800
BccI CCATC 1 cut(s) 203
BceAI ACGGC 2 cut(s) 249, 399
BcoDI GTCTC 3 cut(s) 27, 601, 1056
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 1 cut(s) 730
BisI GCNGC 1 cut(s) 789
BlsI GCNGC 1 cut(s) 790
Bme18I GGWCC 1 cut(s) 622
BmeRI GACNNNNNGTC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 2 cut(s) 25, 623
BpiI GAAGAC 1 cut(s) 899
BpmI CTGGAG 1 cut(s) 176
Bpu14I TTCGAA 1 cut(s) 1150
Bsa29I ATCGAT 1 cut(s) 845
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 331, 450, 753
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bse3DI GCAATG 1 cut(s) 798
BseCI ATCGAT 1 cut(s) 845
BseDI CCNNGG 3 cut(s) 331, 450, 753
BseGI GGATG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 671
BseXI GCAGC 1 cut(s) 800
BshFI GGCC 2 cut(s) 64, 968
BshVI ATCGAT 1 cut(s) 845
BsiHKAI GWGCWC 1 cut(s) 889
BslFI GGGAC 2 cut(s) 635, 1287
BsmAI GTCTC 3 cut(s) 27, 601, 1056
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 635, 1287
BsnI GGCC 2 cut(s) 64, 968
Bsp119I TTCGAA 1 cut(s) 1150
Bsp1286I GDGCHC 1 cut(s) 889
BspANI GGCC 2 cut(s) 64, 968
BspCNI CTCAG 1 cut(s) 42
BspDI ATCGAT 1 cut(s) 845
BspLI GGNNCC 2 cut(s) 25, 623
BspPI GGATC 5 cut(s) 112, 312, 471, 804, 850
BspT104I TTCGAA 1 cut(s) 1150
BsrDI GCAATG 1 cut(s) 798
BssECI CCNNGG 3 cut(s) 331, 450, 753
BssT1I CCWWGG 1 cut(s) 331
Bst4CI ACNGT 6 cut(s) 38, 544, 618, 767, 1204, 1298
Bst6I CTCTTC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 1150
BstDEI CTNAG 3 cut(s) 29, 747, 1167
BstF5I GGATG 1 cut(s) 79
BstMAI GTCTC 3 cut(s) 27, 601, 1056
BstMWI GCNNNNNNNGC 1 cut(s) 725
BstNSI RCATGY 2 cut(s) 422, 927
BstSFI CTRYAG 1 cut(s) 730
BstV1I GCAGC 1 cut(s) 800
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 2 cut(s) 463, 796
BstYI RGATCY 2 cut(s) 463, 796
Bsu15I ATCGAT 1 cut(s) 845
BsuRI GGCC 2 cut(s) 64, 968
BsuTUI ATCGAT 1 cut(s) 845
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 549, 614
Cfr13I GGNCC 1 cut(s) 622
ClaI ATCGAT 1 cut(s) 845
Csp6I GTAC 2 cut(s) 17, 857
CspCI CAANNNNNGTGG 6 cut(s) 82, 117, 599, 634, 958, 993
CviAII CATG 3 cut(s) 419, 653, 924
CviJI RGCY 9 cut(s) 5, 64, 236, 386, 719, 728, 887, 968, 1285
CviKI_1 RGCY 9 cut(s) 5, 64, 236, 386, 719, 728, 887, 968, 1285
CviQI GTAC 2 cut(s) 17, 857
DdeI CTNAG 3 cut(s) 29, 747, 1167
DraIII CACNNNGTG 1 cut(s) 269
DriI GACNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 1 cut(s) 966
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 887
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 887
EcoICRI GAGCTC 1 cut(s) 887
EcoT14I CCWWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 889
ErhI CCWWGG 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 3 cut(s) 422, 656, 927
FaqI GGGAC 2 cut(s) 635, 1287
FatI CATG 3 cut(s) 418, 652, 923
Fnu4HI GCNGC 1 cut(s) 789
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 1 cut(s) 789
FspBI CTAG 1 cut(s) 666
GluI GCNGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 64, 968
Hin1II CATG 3 cut(s) 422, 656, 927
HincII GTYRAC 2 cut(s) 823, 1000
HindII GTYRAC 2 cut(s) 823, 1000
HinfI GANTC 1 cut(s) 983
HpaI GTTAAC 2 cut(s) 823, 1000
HphI GGTGA 4 cut(s) 252, 404, 466, 878
Hpy166II GTNNAC 5 cut(s) 663, 823, 1000, 1070, 1186
Hpy188I TCNGA 5 cut(s) 32, 309, 361, 754, 1018
Hpy188III TCNNGA 4 cut(s) 10, 164, 467, 1272
Hpy8I GTNNAC 5 cut(s) 663, 823, 1000, 1070, 1186
HpyAV CCTTC 5 cut(s) 267, 454, 1121, 1146, 1257
HpyCH4III ACNGT 6 cut(s) 38, 544, 618, 767, 1204, 1298
HpyCH4IV ACGT 2 cut(s) 19, 1256
HpyCH4V TGCA 8 cut(s) 124, 424, 652, 851, 1009, 1090, 1106, 1219
HpyF10VI GCNNNNNNNGC 1 cut(s) 725
HpyF3I CTNAG 3 cut(s) 29, 747, 1167
HpySE526I ACGT 2 cut(s) 19, 1256
Hsp92II CATG 3 cut(s) 422, 656, 927
KspAI GTTAAC 2 cut(s) 823, 1000
LmnI GCTCC 1 cut(s) 513
LpnPI CCDG 8 cut(s) 206, 387, 428, 653, 837, 972, 1020, 1293
Lsp1109I GCAGC 1 cut(s) 800
MaeI CTAG 1 cut(s) 666
MaeII ACGT 2 cut(s) 19, 1256
MaeIII GTNAC 5 cut(s) 281, 410, 559, 701, 776
MboII GAAGA 8 cut(s) 70, 152, 446, 469, 491, 899, 1005, 1136
MflI RGATCY 2 cut(s) 463, 796
MhlI GDGCHC 1 cut(s) 889
MlsI TGGCCA 1 cut(s) 968
MluNI TGGCCA 1 cut(s) 968
MnlI CCTC 8 cut(s) 38, 54, 295, 333, 445, 649, 748, 939
Mox20I TGGCCA 1 cut(s) 968
Mph1103I ATGCAT 1 cut(s) 654
MroXI GAANNNNTTC 1 cut(s) 1212
MscI TGGCCA 1 cut(s) 968
MseI TTAA 5 cut(s) 174, 783, 822, 999, 1109
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 968
MwoI GCNNNNNNNGC 1 cut(s) 725
NlaIII CATG 3 cut(s) 422, 656, 927
NlaIV GGNNCC 2 cut(s) 25, 623
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 2 cut(s) 422, 927
NspV TTCGAA 1 cut(s) 1150
PciI ACATGT 1 cut(s) 923
PdmI GAANNNNTTC 1 cut(s) 1212
PfeI GAWTC 1 cut(s) 983
PflFI GACNNNGTC 1 cut(s) 748
PkrI GCNGC 1 cut(s) 790
Ppu21I YACGTR 1 cut(s) 20
PscI ACATGT 1 cut(s) 923
Psp124BI GAGCTC 1 cut(s) 889
PspN4I GGNNCC 2 cut(s) 25, 623
PspPI GGNCC 1 cut(s) 622
PsrI GAACNNNNNNTAC 2 cut(s) 1197, 1229
PsuI RGATCY 2 cut(s) 463, 796
PsyI GACNNNGTC 1 cut(s) 748
RsaI GTAC 2 cut(s) 18, 858
RsaNI GTAC 2 cut(s) 17, 857
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 889
SaqAI TTAA 5 cut(s) 174, 783, 822, 999, 1109
SatI GCNGC 1 cut(s) 789
Sau96I GGNCC 1 cut(s) 622
SduI GDGCHC 1 cut(s) 889
SfcI CTRYAG 1 cut(s) 730
SfuI TTCGAA 1 cut(s) 1150
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 417
SpeI ACTAGT 1 cut(s) 665
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 1 cut(s) 889
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 6 cut(s) 38, 544, 618, 767, 1204, 1298
TaiI ACGT 2 cut(s) 22, 1259
TaqI TCGA 6 cut(s) 56, 477, 845, 877, 913, 1150
TatI WGTACW 1 cut(s) 856
TfiI GAWTC 1 cut(s) 983
Tru1I TTAA 5 cut(s) 174, 783, 822, 999, 1109
Tru9I TTAA 5 cut(s) 174, 783, 822, 999, 1109
TscAI CASTG 2 cut(s) 549, 621
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 5 cut(s) 378, 845, 899, 1049, 1278
TspGWI ACGGA 1 cut(s) 969
TspRI CASTG 2 cut(s) 549, 621
Tth111I GACNNNGTC 1 cut(s) 748
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 5 cut(s) 85, 249, 397, 960, 1250
XceI RCATGY 2 cut(s) 422, 927
XmnI GAANNNNTTC 1 cut(s) 1212
XspI CTAG 1 cut(s) 666
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.