RLG00000026824

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002400
Physical Location & Seq
Forward (+)
6298719 .. 6299840
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026824

Sequence Viewer

Length: 1122 bp
ATGGGTACAATGAAAACACCTGAAAAAGAAATTGGGTTTAGTCCCAATTTTAAGAGGCCTAGAGTTTATGCAGTTCGAGATTTCCCTGAAGGTTGTGGACGATTTGTGGCTCAAGTGCAGCCAAGTGAATGTGAAGAGGATGATAAAAATATGGACACATTACAAAGGAAAAATCGCTTCCTGCCTAGTCCTCGATTTGATGTTGATGGTGTTCGAGATTTTCCTAAAGGTTGTGGACCGATGGTAGTTTCTTCTTTGAAAGATGCTGAAGAGAAGAGTTTGGTTCCAGACGTCAAGGATAAAGAATGCGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGTGAAGAAAGCTTTGGCTGCATATCAAGAAAAGAGTTTGGTTCCAGACGTCAAGGATAAAGAATGTGAGCAGCGTGTTGTTGGTCACAAGTTGAGTGTTGCAAGTGTGGTTGATAAAGCTCATAACAAGGTGAAGAAAGCTTTGGCTTCATATCAAGAGACTTTGATTGAGATGGGTGGAGAATGCGGTGCTAATTTCAACAGACTAAAGGGTTGGAAATTGTATGTTGAAGCAGCAATGTATCTTAAGAAGAAAGATAAATGGGTTAACACGATGAAACAAACAGGGGACATTCCAGGAGTAAAGGTTGGTGACAAGTTTCGGTATAGACCTGAATTAGCTATTGTTGGTCTTCATAGTCACTTTCAATGCGGTATTGATTATATGACAGAGGACGGGGAAATGATTGCCACAAGTATTGTTGACTCTGGCCGCTATGCGAACGAGACAGAATCACCTGATGTCATGATATACACAGGTCAAGGTGGAAATCCAAATGTTTCAAAAGGGGAACCAATAGATCAAAAGCCCGAGCGTGGAAACCGTGCACTGATGAATAACAAGGCAGCAGGAACTCCTGTGAGGGTAATCCGGAGCTATAAATTTCGGGAGGAATCTGGTTATGTTTATGATGGTCTCTACATTGTTGATGATTTTTGGCGAGAAAGAGGGGATTATGGTAAATTTGTGTTTAGATTTTCGTTGAGGAGGATTTCGGGTCAACCAAAACTCACACTTCGCAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

374

Amino Acids

42.08

Weight (kDa)

9.05

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 219 - 365 4e-47 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 294, 423
AccIII TCCGGA 1 cut(s) 963
AciI CCGC 3 cut(s) 558, 744, 805
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 2 cut(s) 974, 1055
AcuI CTGAAG 2 cut(s) 108, 288
AcyI GRCGYC 2 cut(s) 291, 420
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 908
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 5 cut(s) 259, 571, 602, 740, 876
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 6 cut(s) 15, 47, 368, 400, 497, 529
AluBI AGCT 6 cut(s) 362, 383, 491, 512, 713, 969
AluI AGCT 6 cut(s) 362, 383, 491, 512, 713, 969
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 3 cut(s) 524, 812, 1013
Alw44I GTGCAC 1 cut(s) 918
Ama87I CYCGRG 1 cut(s) 902
Aor13HI TCCGGA 1 cut(s) 963
AoxI GGCC 2 cut(s) 56, 802
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 6 cut(s) 118, 313, 389, 442, 605, 938
ApoI RAATTY 2 cut(s) 974, 1055
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 4 cut(s) 385, 514, 695, 819
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 922
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 6 cut(s) 130, 325, 376, 454, 617, 950
BccI CCATC 4 cut(s) 200, 235, 538, 998
BciT130I CCWGG 1 cut(s) 669
BcoDI GTCTC 3 cut(s) 524, 812, 1013
BfaI CTAG 2 cut(s) 60, 186
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 7 cut(s) 119, 314, 390, 443, 606, 805, 939
BlsI GCNGC 7 cut(s) 120, 315, 391, 444, 607, 806, 940
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 902
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 285, 414, 885
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 291, 420
BsaI GGTCTC 1 cut(s) 1013
BsaWI WCCGGW 1 cut(s) 963
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 908
Bse3DI GCAATG 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 963
BseBI CCWGG 1 cut(s) 669
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 908
BseMI GCAATG 1 cut(s) 615
BseRI GAGGAG 1 cut(s) 1093
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 6 cut(s) 130, 325, 376, 454, 617, 950
BsgI GTGCAG 1 cut(s) 137
BshFI GGCC 2 cut(s) 58, 804
BsiHKAI GWGCWC 1 cut(s) 922
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 1 cut(s) 964
BslFI GGGAC 2 cut(s) 27, 674
BslI CCNNNNNNNGG 1 cut(s) 908
BsmAI GTCTC 3 cut(s) 524, 812, 1013
BsmFI GGGAC 2 cut(s) 27, 674
BsmI GAATGC 2 cut(s) 311, 560
BsnI GGCC 2 cut(s) 58, 804
Bso31I GGTCTC 1 cut(s) 1013
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 922
Bsp13I TCCGGA 1 cut(s) 963
Bsp143I GATC 1 cut(s) 892
BspACI CCGC 3 cut(s) 558, 744, 805
BspANI GGCC 2 cut(s) 58, 804
BspEI TCCGGA 1 cut(s) 963
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 285, 414, 885
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 1013
BsrDI GCAATG 1 cut(s) 615
BssMI GATC 1 cut(s) 892
BssNI GRCGYC 2 cut(s) 291, 420
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 917, 1118
Bst6I CTCTTC 3 cut(s) 129, 264, 269
BstACI GRCGYC 2 cut(s) 291, 420
BstAFI CTTAAG 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 311
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 1 cut(s) 895
BstMAI GTCTC 3 cut(s) 524, 812, 1013
BstMBI GATC 1 cut(s) 892
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 667
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 6 cut(s) 130, 325, 376, 454, 617, 950
BstV2I GAAGAC 1 cut(s) 716
BsuRI GGCC 2 cut(s) 58, 804
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 920
Cac8I GCNNGC 1 cut(s) 311
CciI TCATGA 1 cut(s) 837
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 6
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviAII CATG 1 cut(s) 838
CviQI GTAC 1 cut(s) 6
DpnI GATC 1 cut(s) 894
DpnII GATC 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 3 cut(s) 129, 264, 269
EarI CTCTTC 3 cut(s) 129, 264, 269
Eco147I AGGCCT 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 1013
Eco47I GGWCC 1 cut(s) 236
Eco57I CTGAAG 2 cut(s) 108, 288
Eco88I CYCGRG 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 667
FaeI CATG 1 cut(s) 841
FaqI GGGAC 2 cut(s) 27, 674
FatI CATG 1 cut(s) 837
Fnu4HI GCNGC 7 cut(s) 119, 314, 390, 443, 606, 805, 939
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 7 cut(s) 119, 314, 390, 443, 606, 805, 939
FspBI CTAG 2 cut(s) 60, 186
GluI GCNGC 7 cut(s) 119, 314, 390, 443, 606, 805, 939
HaeIII GGCC 2 cut(s) 58, 804
HapII CCGG 1 cut(s) 964
Hin1I GRCGYC 2 cut(s) 291, 420
Hin1II CATG 1 cut(s) 841
HincII GTYRAC 3 cut(s) 640, 796, 1094
HindII GTYRAC 3 cut(s) 640, 796, 1094
HindIII AAGCTT 2 cut(s) 381, 510
HinfI GANTC 3 cut(s) 797, 824, 986
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 1 cut(s) 964
HphI GGTGA 4 cut(s) 385, 514, 695, 819
Hpy166II GTNNAC 6 cut(s) 98, 236, 640, 796, 920, 1094
Hpy188III TCNNGA 9 cut(s) 77, 215, 287, 398, 416, 527, 838, 964, 980
Hpy8I GTNNAC 6 cut(s) 98, 236, 640, 796, 920, 1094
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 2 cut(s) 917, 1118
HpyCH4IV ACGT 2 cut(s) 291, 420
HpyCH4V TGCA 6 cut(s) 71, 118, 344, 392, 473, 920
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpySE526I ACGT 2 cut(s) 291, 420
Hsp92I GRCGYC 2 cut(s) 291, 420
Hsp92II CATG 1 cut(s) 841
Kpn2I TCCGGA 1 cut(s) 963
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 1 cut(s) 892
LmnI GCTCC 1 cut(s) 966
Lsp1109I GCAGC 6 cut(s) 130, 325, 376, 454, 617, 950
LweI GCATC 1 cut(s) 253
MaeI CTAG 2 cut(s) 60, 186
MaeII ACGT 2 cut(s) 291, 420
MaeIII GTNAC 4 cut(s) 326, 455, 683, 731
MalI GATC 1 cut(s) 894
MboI GATC 1 cut(s) 892
MboII GAAGA 8 cut(s) 146, 243, 281, 286, 388, 517, 634, 716
MhlI GDGCHC 1 cut(s) 922
MluCI AATT 7 cut(s) 30, 46, 565, 590, 707, 974, 1055
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 9 cut(s) 48, 130, 201, 757, 948, 976, 1034, 1071, 1074
MroI TCCGGA 1 cut(s) 963
MseI TTAA 4 cut(s) 51, 618, 639, 1120
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 964
MspR9I CCNGG 1 cut(s) 669
Mva1269I GAATGC 2 cut(s) 311, 560
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 1 cut(s) 892
NlaIII CATG 1 cut(s) 841
NlaIV GGNNCC 3 cut(s) 285, 414, 885
NmuCI GTSAC 4 cut(s) 326, 455, 683, 731
PagI TCATGA 1 cut(s) 837
PceI AGGCCT 1 cut(s) 58
PctI GAATGC 2 cut(s) 311, 560
PfeI GAWTC 2 cut(s) 824, 986
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 7 cut(s) 120, 315, 391, 444, 607, 806, 940
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 285, 414, 885
PspPI GGNCC 1 cut(s) 236
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SaqAI TTAA 4 cut(s) 51, 618, 639, 1120
SatI GCNGC 7 cut(s) 119, 314, 390, 443, 606, 805, 939
Sau3AI GATC 1 cut(s) 892
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 1 cut(s) 922
SfaNI GCATC 1 cut(s) 253
SinI GGWCC 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 111, 617
SmoI CTYRAG 2 cut(s) 111, 617
Sse9I AATT 7 cut(s) 30, 46, 565, 590, 707, 974, 1055
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 3 cut(s) 558, 744, 805
SspMI CTAG 2 cut(s) 60, 186
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 1 cut(s) 667
TaaI ACNGT 2 cut(s) 917, 1118
TaiI ACGT 2 cut(s) 294, 423
TaqI TCGA 3 cut(s) 76, 193, 214
TaqII GACCGA 1 cut(s) 253
TasI AATT 7 cut(s) 30, 46, 565, 590, 707, 974, 1055
TauI GCSGC 1 cut(s) 807
TfiI GAWTC 2 cut(s) 824, 986
Tru1I TTAA 4 cut(s) 51, 618, 639, 1120
Tru9I TTAA 4 cut(s) 51, 618, 639, 1120
TscAI CASTG 1 cut(s) 927
TseFI GTSAC 4 cut(s) 326, 455, 683, 731
TseI GCWGC 6 cut(s) 118, 313, 389, 442, 605, 938
Tsp45I GTSAC 4 cut(s) 326, 455, 683, 731
TspDTI ATGAA 5 cut(s) 26, 510, 662, 716, 941
TspRI CASTG 1 cut(s) 927
Vha464I CTTAAG 1 cut(s) 617
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 2 cut(s) 974, 1055
XspI CTAG 2 cut(s) 60, 186
ZraI GACGTC 2 cut(s) 292, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.