Rroxscaffold_4G00324280

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002400
Physical Location & Seq
Forward (+)
55622392 .. 55623357
966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324280.1

Sequence Viewer

Length: 864 bp
ATGGCCATAGTGAAAGTACATGGAACAATACACCATGATTGCTTTATTCCCCTGAAGTGGGCAAGAGTTGATGTTATTCGACACCTTCCTAGAGGGCGCGGATCAACTGTTAGGCGGCAAAAGGGTGAATGCGAAGATATTGATTATATCGTTGAACCTACTAATAAAGATAATGAACCTTCTGCGAAGATTGATAAGGTGTGTGCGTCTCGTGAAAGGATTGATAAGGAAAATCAGTATACAAGCTACACAATATTTAGCAAGGTCGCACCAAGTTCTATTGCCTCAAATAATTGTCAGAGACTGGAGAGCAAAAGTTTACCACCACCGGATCGCCAATGCCATAATGAGAAGGTTTCTGTTGCTAGTTACCGGTTGACTGATCCTGATGTGGAGTCCAAAGATCGTTGCAAGGTGAAGAAGACGCTTAATGCATTTAAGGAGATACTGACCAAGTTCAACAAACGAAATTGCTCAACTTACATTTTAGCAGCAATTCGTTTAAGGAATGAAGGAAAATGGCTCAACATGAGGAAACAATTAGGCGCCATTCGAGGAGTAAAAGTTGGTGACAAGTTTCGATATAGAGCTCAACTAAATATCGTTGGGCTTCATCAACATTTCACCAATGGAATTGATTATATAAAGAGCAAAGATGGGAAAATGCTAGTCACAAGTATTGTTGATTCTGGTCGTTATGCAAACAATATGCAATGTTCCGATGTATTGATCTATTCTGGTGAAGGTGGAAATTCTCCGTTTAATAAATTGAAACCAACAGATCAGACACTTGAGCGTGGAAACCTTGCATTGAAGAATAGCAAAGAAGCACAAACTTTCAAAAGAAAGGGGGCAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SAD_SRA SET

Protein Analysis

287

Amino Acids

32.64

Weight (kDa)

9.71

Isoelectric Point (pI)

22.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 181 - 278 1e-25 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 239
AccII CGCG 1 cut(s) 99
AciI CCGC 2 cut(s) 99, 115
AclWI GGATC 3 cut(s) 109, 339, 377
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 751
AcuI CTGAAG 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 546
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 57, 58
AgeI ACCGGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 155, 460, 772, 814, 841
AluBI AGCT 2 cut(s) 246, 590
AluI AGCT 2 cut(s) 246, 590
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 2 cut(s) 213, 295
AlwI GGATC 3 cut(s) 109, 339, 377
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 751
AsiGI ACCGGT 1 cut(s) 372
AspLEI GCGC 2 cut(s) 99, 548
AsuHPI GGTGA 5 cut(s) 137, 427, 581, 616, 752
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 592
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 503
BccI CCATC 1 cut(s) 650
BcoDI GTCTC 2 cut(s) 213, 295
BfaI CTAG 3 cut(s) 90, 366, 668
BfoI RGCGCY 1 cut(s) 549
BisI GCNGC 2 cut(s) 116, 492
BlsI GCNGC 2 cut(s) 117, 493
BmiI GGNNCC 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 812
BsaHI GRCGYC 1 cut(s) 546
BsaWI WCCGGW 2 cut(s) 328, 372
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 58
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 719
BseLI CCNNNNNNNGG 2 cut(s) 57, 58
BseMI GCAATG 1 cut(s) 719
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 545
BshTI ACCGGT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 2 cut(s) 329, 373
BslI CCNNNNNNNGG 2 cut(s) 57, 58
BsmAI GTCTC 2 cut(s) 213, 295
BsmBI CGTCTC 1 cut(s) 213
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 6 cut(s) 101, 331, 382, 403, 729, 781
BspACI CCGC 2 cut(s) 99, 115
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 547
BspPI GGATC 3 cut(s) 109, 339, 377
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 719
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 1 cut(s) 309
BssAI RCCGGY 1 cut(s) 372
BssMI GATC 6 cut(s) 101, 331, 382, 403, 729, 781
BssNAI GTATAC 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 546
BssSI CACGAG 1 cut(s) 210
Bst1107I GTATAC 1 cut(s) 240
Bst2BI CACGAG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 546
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 2 cut(s) 99, 548
BstKTI GATC 6 cut(s) 104, 334, 385, 406, 732, 784
BstMAI GTCTC 2 cut(s) 213, 295
BstMBI GATC 6 cut(s) 101, 331, 382, 403, 729, 781
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 503
BstV2I GAAGAC 1 cut(s) 428
BstZ17I GTATAC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 5
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 2 cut(s) 99, 548
Cfr10I RCCGGY 1 cut(s) 372
CseI GACGC 2 cut(s) 195, 433
Csp6I GTAC 1 cut(s) 17
CspAI ACCGGT 1 cut(s) 372
CviAII CATG 3 cut(s) 20, 35, 529
CviJI RGCY 5 cut(s) 5, 246, 523, 590, 610
CviKI_1 RGCY 5 cut(s) 5, 246, 523, 590, 610
CviQI GTAC 1 cut(s) 17
DinI GGCGCC 1 cut(s) 547
DpnI GATC 6 cut(s) 103, 333, 384, 405, 731, 783
DpnII GATC 6 cut(s) 101, 331, 382, 403, 729, 781
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 592
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 590
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 592
EgeI GGCGCC 1 cut(s) 547
EheI GGCGCC 1 cut(s) 547
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 3 cut(s) 23, 38, 532
FatI CATG 3 cut(s) 19, 34, 528
FblI GTMKAC 1 cut(s) 239
Fnu4HI GCNGC 2 cut(s) 116, 492
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 2 cut(s) 116, 492
FspBI CTAG 3 cut(s) 90, 366, 668
GlaI GCGC 2 cut(s) 98, 547
GluI GCNGC 2 cut(s) 116, 492
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 329, 373
HgaI GACGC 2 cut(s) 195, 433
HhaI GCGC 2 cut(s) 99, 548
Hin1I GRCGYC 1 cut(s) 546
Hin1II CATG 3 cut(s) 23, 38, 532
Hin6I GCGC 2 cut(s) 97, 546
HinP1I GCGC 2 cut(s) 97, 546
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 2 cut(s) 395, 686
HpaII CCGG 2 cut(s) 329, 373
HphI GGTGA 5 cut(s) 137, 427, 581, 616, 752
Hpy166II GTNNAC 3 cut(s) 240, 320, 378
Hpy188I TCNGA 3 cut(s) 300, 721, 786
Hpy188III TCNNGA 2 cut(s) 212, 386
Hpy8I GTNNAC 3 cut(s) 240, 320, 378
HpyAV CCTTC 5 cut(s) 95, 189, 346, 506, 737
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 5 cut(s) 411, 434, 701, 712, 809
Hsp92I GRCGYC 1 cut(s) 546
Hsp92II CATG 3 cut(s) 23, 38, 532
HspAI GCGC 2 cut(s) 97, 546
KasI GGCGCC 1 cut(s) 545
Kzo9I GATC 6 cut(s) 101, 331, 382, 403, 729, 781
LpnPI CCDG 7 cut(s) 65, 290, 342, 386, 399, 675, 723
Lsp1109I GCAGC 1 cut(s) 503
MaeI CTAG 3 cut(s) 90, 366, 668
MaeIII GTNAC 3 cut(s) 368, 569, 670
MalI GATC 6 cut(s) 103, 333, 384, 405, 731, 783
MboI GATC 6 cut(s) 101, 331, 382, 403, 729, 781
MboII GAAGA 5 cut(s) 146, 199, 430, 433, 826
MhlI GDGCHC 1 cut(s) 592
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 8 cut(s) 292, 469, 495, 539, 633, 751, 767, 855
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 546
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 4 cut(s) 86, 295, 525, 548
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 436
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 429, 438, 503, 762
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 329, 373
Mva1269I GAATGC 1 cut(s) 134
MvnI CGCG 1 cut(s) 99
NarI GGCGCC 1 cut(s) 546
NdeII GATC 6 cut(s) 101, 331, 382, 403, 729, 781
NlaIII CATG 3 cut(s) 23, 38, 532
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 569, 670
NsiI ATGCAT 1 cut(s) 436
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 2 cut(s) 117, 493
PleI GAGTC 1 cut(s) 403
PluTI GGCGCC 1 cut(s) 549
PpsI GAGTC 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 304
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SacI GAGCTC 1 cut(s) 592
SaqAI TTAA 4 cut(s) 429, 438, 503, 762
SatI GCNGC 2 cut(s) 116, 492
Sau3AI GATC 6 cut(s) 101, 331, 382, 403, 729, 781
SchI GAGTC 1 cut(s) 404
SduI GDGCHC 1 cut(s) 592
SfoI GGCGCC 1 cut(s) 547
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
Sse9I AATT 8 cut(s) 292, 469, 495, 539, 633, 751, 767, 855
SsiI CCGC 2 cut(s) 99, 115
SspDI GGCGCC 1 cut(s) 545
SspI AATATT 1 cut(s) 255
SspMI CTAG 3 cut(s) 90, 366, 668
SstI GAGCTC 1 cut(s) 592
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 3 cut(s) 79, 553, 580
TasI AATT 8 cut(s) 292, 469, 495, 539, 633, 751, 767, 855
TatI WGTACW 1 cut(s) 16
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 4 cut(s) 429, 438, 503, 762
Tru9I TTAA 4 cut(s) 429, 438, 503, 762
TseFI GTSAC 2 cut(s) 569, 670
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 2 cut(s) 569, 670
TspDTI ATGAA 3 cut(s) 189, 525, 602
TspGWI ACGGA 1 cut(s) 747
XapI RAATTY 1 cut(s) 751
XmiI GTMKAC 1 cut(s) 239
XspI CTAG 3 cut(s) 90, 366, 668
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.